Improvement and breeding
B. Yoosefi; S.R. Tabaie-Aghdaie; A. Amiri
Abstract
Forty eight damask rose (Rosa damascena Mill.) accessions from different parts of Iran (11 native accessions of Kermanshah and 37 accessions from other provinces of the country) were cultivated in a randomized complete block design with three replications in Mehregan research station, Kermanshah province ...
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Forty eight damask rose (Rosa damascena Mill.) accessions from different parts of Iran (11 native accessions of Kermanshah and 37 accessions from other provinces of the country) were cultivated in a randomized complete block design with three replications in Mehregan research station, Kermanshah province in 2006-2011 and their yield, morphological, and phenological traits were studied. Each experimental plot consisted of three individual plants with a distance of 3 m × 3 m. The drip irrigation was carried out once every 10 days. Also, the chemical fertilizers and pesticides were not used in the field. The desired traits were recorded and analyzed after the seedlings establishment in the third to fifth years. The results of combined analysis of variance showed that all the studied traits were significantly different (P<0.01) between the experimental years and between the different accessions. The variance of accession × year interaction in all the traits was significant (P<0.01). According to the results, the accessions Kermanshah codes 10, 11, 2, and 3, Kurdistan 1, and Isfahan codes 7 and 4 could be introduced for the cultivation in the climatic conditions of Kermanshah province as the compatible accessions with suitable flower and essential oil yield.
A. Saremi-Rad; A. Mohammadi
Abstract
Damask rose (Rosa damascena Mill.) is one of the most important plants in the pharmaceutical and perfumery industry. The present study was carried out to achieve an efficient and effective regeneration instruction for use in breeding programs under laboratory conditions of this economically valuable ...
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Damask rose (Rosa damascena Mill.) is one of the most important plants in the pharmaceutical and perfumery industry. The present study was carried out to achieve an efficient and effective regeneration instruction for use in breeding programs under laboratory conditions of this economically valuable plant. For this purpose, two damask rose genotypes were used in MS and WPM media. In the proliferation stage, the effect of growth regulator 6-benzylaminopurine (BAP) at six levels of 0 (control), 1, 2, 3, 4 and 5 mg.l-1, and in the rooting stage, the effect of growth regulator indole-3-butyric acid (IBA) at five levels of 0 (control), 0.01, 0.05, 0.1 and 0.2 mg.l-1 were investigated. Based on the ANOVA results of proliferation data, genotypes Kashan in MS medium (2.72) and Isfahan in the WPM medium (2.50) had the highest number of shoots. The highest amount of shooting was obtained in MS medium containing 5 mg.l-1 BAP. Genotype Kashan in MS medium containing 5 mg.l-1 BAP had the highest mean shoot length (2.93 cm). In terms of shoots fresh weight, genotype Isfahan had the highest weight in the WPM medium containing 5 mg.l-1 BAP. The interaction effect of genotype × medium × BAP on dry weight was not significant. The highest number and mean root length in both genotypes Isfahan and Kashan were observed in the WPM medium containing 0.2 mg.l-1 IBA. Rooted shoots were transferred to the pots containing a mixture of horticultural soil, peat, and perlite with the same volume ratio (1: 1: 1), and based on observation, the survival coefficient was more than 80%.
M. Shahsavari; D. Naderi; M.M. Gheisari
Abstract
Balanced nutrition is one of the most important factors in improving the quality and performance of medicinal plants such as Domask-Rose (Rosa damascena Mill.). In recent years, the approach to removing or reducing the use of chemical fertilizers with the aim of replacing organic fertilizers and ...
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Balanced nutrition is one of the most important factors in improving the quality and performance of medicinal plants such as Domask-Rose (Rosa damascena Mill.). In recent years, the approach to removing or reducing the use of chemical fertilizers with the aim of replacing organic fertilizers and the application of new products has been widely considered. For this purpose, a factorial experiment was conducted in a randomized complete block design with two factors of organic nano-fertilizer (levels of zero, 2, 3 and 4 cc in one liter of water) and humic acid (levels of zero, 1, 2 and 3 cc in one liter of water) in three replications. The results showed that the highest amount of nitrogen was obtained in organic nano-fertilizer treatments of 4 and 3 per thousand, and the highest amount of phosphorus and potassium was obtained in organic nano-fertilizer of 4 per thousand. The highest amount of anthocyanin (62.63 μg / g) was obtained in organic nano-fertilizer of 3 per 1000, and the highest amount of carotenoids (range from 0.44 to 0.35 mg / g) was obtained in humic acid treatments without nano-fertilizer and with nano-fertilizer of 2, 4 and 3 per thousand. The 2-phenyl ethanol, citronellol, geraniol and alpha-pinene compounds were the highest essential oil components. The highest amount of 2-phenyl ethanol was obtained in the treatment of organic nano-fertilizer and humic acid at concentrations of 3 per thousand. The highest amount of citronellol, alpha-pinene and the least amount of 2-pheny lethanol were observed in the treatment of organic nano-fertilizer with 4 per thousand and humic acid 3 per thousand. The lowest amount of citronellol and alpha-pinene, and the highest amount of geraniol were obtained in organic nano-fertilizer 2 per thousand with humic acid 2 and 1 per thousand. According to the findings of this research, organic nano-fertilizer and humic acid treatments showed a positive effect on the concentration of mineral elements and essential oil components.
B. Yoosefi; H.R. Ghasempoor; B. Yousefi; S.R. Tabaeii Aghdaie; K. Jaimand
Abstract
Damask Rose (Rosa damascena Mill.) is an important medicinal and industrial species. To achieve more and better-quality essential oil identifying the superior accessions of Damask Rose and their genetic relationships is very important. For a detailed review of the relationship among the genotypes of ...
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Damask Rose (Rosa damascena Mill.) is an important medicinal and industrial species. To achieve more and better-quality essential oil identifying the superior accessions of Damask Rose and their genetic relationships is very important. For a detailed review of the relationship among the genotypes of this species and variations in the essential oil composition, the use of multivariate statistical analysis could be useful. In this study, 25 different accessions of Damask Rose were collected from Kermanshah province and other regions of Iran and were planted in a randomized complete blocks design. The essential oil was extracted from the petals with hydrodistillation method. Essential oil composition was identified by using the gas chromatography and gas chromatography coupled with mass spectrometry and the amount (percentage) of each composition was measured. Essential oil compositions were evaluated using cluster analysis. According to the results of cluster analysis, the accessions were grouped in four groups. Discriminant analysis confirmed the results of cluster analysis. Component analysis showed that the accessions were in four groups and there were four different trends among the accessions studied for the chemical compositions of essential oils. According to the correlation analysis, the two important factors, geraniol and citronellol, which increased the quality of essential oil compounds in damask rose, showed a significant positive relationship with each other and with citronellyl acetate and geranial.
K. Mahdavi Mashaki; A. Moieni; M. Jalali Javaran
Abstract
Rose is known as the queen of flowers. Rosa damask (Rosa damascena Mill.) is utilized as a main source of rose oil and rose water in Iran. Biotechnology methods are considered as a fast and efficient way to produce haploid plants and pure lines. Among the different methods, androgenesis is considered ...
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Rose is known as the queen of flowers. Rosa damask (Rosa damascena Mill.) is utilized as a main source of rose oil and rose water in Iran. Biotechnology methods are considered as a fast and efficient way to produce haploid plants and pure lines. Among the different methods, androgenesis is considered as the most effective procedure, due to its high quantity of microspores in the anthers. In the present study, the effect of medium composition was evaluated on anther culture in two ecotypes of Damask rose, and miniature rose. The results showed that the interaction between H1 medium and mid-uninucleate stage produced the highest callogenesis in Damask rose, Kashan ecotype. Furthermore, the results showed that removing ammonium nitrate and doubling potassium nitrate in the medium produced higher callogenesis. In miniature rose, the medium containing calcium chloride and calcium nitrate produced higher callogenesis. Also, the amino acids improved callogenesis in anther culture of Damask rose, and miniature rose. Moreover, glycine, glutamine and casein hydrolysate were more effective than other studied amino acids. Sucrose was a better sugar compared to sorbitol for callogenesis in the studied genotypes. The chromosome counting and flowcytometery results illustrated that the produced calli were tetraploid (28 chromosomes).
Z. Masumi; P. Zandi; S.R. Tabaei Aghdaei
Abstract
In order to identify the best genotypes of rose (Rosa damascene Mill.), a field experiment was conducted in a randomized completely blocks design with seven treatments and three replications. Flower number, flower fresh weight, yield, petal fresh weight, petal to flower fresh weight ratio, petal number, ...
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In order to identify the best genotypes of rose (Rosa damascene Mill.), a field experiment was conducted in a randomized completely blocks design with seven treatments and three replications. Flower number, flower fresh weight, yield, petal fresh weight, petal to flower fresh weight ratio, petal number, flower diameter, flowering period, essential oil weight were investigated for seven genotypes of rose (Fars1, Fars2, Darab1, Darab2, Darab3, Estahban1, Meymand). Essential oil was obtained by distillation method and essential oil composition was determined by GC and GC/MS. Data analysis was performed by SAS and Jamp4. According to the results of mean comparisons, the highest yield and flower number were recorded for Darab1, Meymand and Fars2, while the highest amount of essential oil was obtained for Maymand and Fars2. A positive and significant correlation was also found between yield and flower number, leading to the increased essential oil. Citronellol, neral, and n-nonadecane were identified as the major components of the essential oil, respectively found in Darab1, Darab3, and Estahban1.
A. Rahamani; M. Mirza; S.R. Tabaei-Aghdai
Abstract
In this project, the effect of chemical fertilizers, organic manure and their combination on the quality of essential oils of Damask Rosa was investigated in Karadj Alborz Research Station. The study was conducted using randomized complete blocks design with 20 independent treatments and three replications ...
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In this project, the effect of chemical fertilizers, organic manure and their combination on the quality of essential oils of Damask Rosa was investigated in Karadj Alborz Research Station. The study was conducted using randomized complete blocks design with 20 independent treatments and three replications in a loam-clay soil with pH =7.9. In this experiment, nitrogen, phosphorus and potash and a combination of trace elements as fertilizer and cow manure were used. Amount of essential oil and quality of essential oil at different treatments were measured in 3 years. Essential oils of petals were extracted by hydrodistillation method in three consecutive years and oil composition was identified in the first and second years of flowering stage using GC and GC/MS. The results of analysis of variance and mean performance showed that the difference among treatments was significant at 1% level of probability. Treatment N80P80K40 and 30 tons of manure per hectare had the highest amount of essential oil. The average amount of essential oil in three consecutive years in this with 0.35 percent was the highest and in control with 0.23 percent was the lowest value. The highest average oil yield in the three years was recorded for treatment N40P40K40, (1395.58 g/ha), and minimum was obtained for the treatment consisting only 40 tons of manure, (636.23 g/ha). After that, the minimum oil yield was recorded for control treatment (868.98 g/ha) in these three years. The composition of essential oils in the first year of flowering showed that Linalool, Geraniol and Citronelllol increased 88, 39 and 36 percent, respectively in treatment N40P40K40, compared with the control. In the second year of flowering, the amount of Geraniol in this treatment increased by 45 percent and that of hexadekanol and heptadekan decreased 45 percent and 30 percent compared with the control.
Z. Nemati Lafmajani; S.R. Tabaei-Aghdaei; M.H. Lebaschi; A.A. Jafari; A. Najafi Ashtiani; M. Daneshkhah
Abstract
In this research, effects of different traits on flower yield of 12 Rosa damascena Mill. accessions from different regions of Iran were analyzed under irrigation (control) and non-irrigation with and without mulch conditions. Randomized complete block designs were applied with three replications at Research ...
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In this research, effects of different traits on flower yield of 12 Rosa damascena Mill. accessions from different regions of Iran were analyzed under irrigation (control) and non-irrigation with and without mulch conditions. Randomized complete block designs were applied with three replications at Research Station of Homand Absard, Damavand, Iran. Flower weight, flower number, flower dry matter percentage, oil percentage, oil yield (per ha), petal weight, plant height and canopy cover were recorded. According to the correlation analysis, flower yield positively correlated with petal weight under non-irrigation with mulch, irrigated (control) and non-irrigation without mulch conditions. The statistical multivariate methods were also applied included multivariate linear regression and path analyses. Using three separate stepwise-regression analysis made it possible to determine the traits with most important impacts on the flower yield, under irrigation (control) and non-irrigation with and without mulch conditions. Flower number, petal weight, oil yield, and oil percentage under non-irrigation without mulch totally explained 91.7% of the variation among genotypes in the regression model. Petal weight, plant cover, and diameter of the species under irrigation (control) totally explained 98.6% of the variation among genotypes in the regression model. Oil percentage, oil yield, petal weight, plant height and flower number per ha under non-irrigation with mulch totally explained 90.6% of the variation among genotypes in the regression model. The results indicated that petal weight has the most direct impact on flower yield based on the path analysis and oil yield showed a negative direct effect on the flower yield under non-irrigation (without mulch) condition. Oil percentage, oil yield, petal weight, plant height and flower number per plant under non-irrigation with mulch, irrigated (control) and non-irrigation without mulch totally explained 95/5% of the variation among genotypes in the regression model. Petal weight had the highest direct impact on flower yield based on the path analysis under irrigation and non-irrigation conditions. Therefore, it is possible to consider petal weight as a magnificent character to improve flower yield of Rosa damascene.
K. Jaimand; M.B. Rezaee; S.R. Tabaei Aghdaei; M. Nadery Hajibagher Kandy; S. Meshkizadeh
Abstract
In this study, tannin was extracted from rose water, wastewater and petal residue of Rosa damascena Mill. Twenty one accessions collected from different provinces of Iran and cultivated in the Research Institute of Forests and Rangelands were sampled for extraction. Then, each sample of essential oil, ...
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In this study, tannin was extracted from rose water, wastewater and petal residue of Rosa damascena Mill. Twenty one accessions collected from different provinces of Iran and cultivated in the Research Institute of Forests and Rangelands were sampled for extraction. Then, each sample of essential oil, rose water, wastewater and petal residue were prepared. The amount of tannin in the samples was measured by colorimetric method and spectrophotometer apparatus (Model 340 Hitachi) at 760 nm. The highest amount of tannin in rose water, wastewater and petal residue was respectively obtained from Khuzistan and Ilam (2163 ppm), Qom (1846 ppm), and Gillan (1432 ppm) accessions for rose water, Zanjan (1919 ppm), Mazandaran (1895 ppm) Qom, Gilan, Lorestan and Isfahan (1871 ppm) accessions for wastewater, and Yazd (3432 ppm), Sistan and Baluchestan (3139 ppm) and Kohgiluyeh-Boyer Ahmad (2993 ppm) accessions for petal residual. According to the results, it is recommended to extract tannin from samples used in rose water production. In other words, this will be economically useful for rose water traditional producers.
M.H. Assareh; Z. Abravesh; S.R. Tabaei Aghdaei
Abstract
Because of the economic and medicinal importance of Rosa damascena Mill. the propagation of this plant was considered. In order to protect the selected genotypes, this experiment was performed by simple layering method during autumn and winter seasons 2007 on new growth twigsof Rosa damascena mature ...
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Because of the economic and medicinal importance of Rosa damascena Mill. the propagation of this plant was considered. In order to protect the selected genotypes, this experiment was performed by simple layering method during autumn and winter seasons 2007 on new growth twigsof Rosa damascena mature shrubs. The branches were laid in sand bed, soaked for 24 h by different concentrations of IBA and NAA hormones. The results showed that the rate of rooting was high in sand bedding material in autumn and winter. The highest rooting percentage was %100 with 50 mgl-1 concentration of IBA in autumn and winter seasons and the lowest of rooting percentage was %53.33 with 50 mgl-1 concentration of NAA in autumn season. The rooting branches were cut from the parent plants after 3 months and planted in pots containing soil: sand: peatmoss complex (1:1:1) in greenhouse conditions, survival percentage was 90%.
K. Jaimand; M.B. Rezaee; M.H. Asareh; S.R. Tabaei Aghdaei; S. Meshkizadeh
Abstract
Flavonoid components are the largest phenolic groups in nature. Pigments are primarily responsible for the color of rose petals (the colorless flavonoids aid the intensification and stabilization of petals color). The aim of this research was extraction and determination of flavonoid compounds kaempferol ...
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Flavonoid components are the largest phenolic groups in nature. Pigments are primarily responsible for the color of rose petals (the colorless flavonoids aid the intensification and stabilization of petals color). The aim of this research was extraction and determination of flavonoid compounds kaempferol and quercetin in petals of Rosa damascena from western of Iran and cultivated in Research Institute of Forests and Rangelands. Samples were collected on late April 2006. A 5 g fresh flower petals was blended with 30 ml of 9:1 methanol:acetic acid, the extract was analyzed by high performance liquid chromatography (HPLC). Two flavonoids kaempferol and quercetin glycoside compounds were extracted from ten samples. The highest kaempferol content were obtained from Kordestan (595 ppm), Qazvine (505 ppm), West Azarbaijan (484 ppm), and Ilam (483 ppm), and the lowest from Kermanshah (74 ppm), Hamedan (88 ppm) and Lorestan (Poldokhtar) (252 ppm). The highest quercetin glucoside contents were obtained from Ardabil (322 ppm), West Azarbaijan (312 ppm), Ilam (302 ppm), and Chahar Mehal (239 ppm), and lowest were from Lorestan (Poldokhtar) (77 ppm), Hamedan (80 ppm), Kermanshah (117 ppm) and Kohkiluyeh (Gachsaran) (158 ppm). According to these results, the best samples were obtained from west Azarbaijan (484 ppm kaempferol, 312 ppm quercetin), Ilam (483 ppm kaempferol, 302 ppm quercetin) and Ardabil (447 ppm kaempferol, 322 ppm quercetin).
