همکاری با انجمن علمی گیاهان دارویی ایران

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش‌آموخته دکترای فیزیولوژی گیاهان زراعی، دانشکده کشاورزی، دانشگاه ایلام، ایلام، ایران

2 استادیار، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه ایلام، ایلام، ایران

3 دانشیار، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه ایلام، ایلام، ایران

چکیده

شناسه دیجیتال (DOR):
98.1000/1735-0905.1400.37.329.106.2.1575.1610

آزمایشی با هدف بررسی نقش پوتریسین و 24-اپی‌براسینولید، بر تغییرات مورفوفیزیولوژیکی در تحمل به کمبود آب در گیاه ریحان (Ocimum basilicum L.) به‌صورت اسپلیت پلات فاکتوریل در قالب طرح پایه بلوک‌های کامل تصادفی با سه تکرار در مزرعه تحقیقاتی دانشکده کشاورزی دانشگاه ایلام در سال‌های زراعی 98-1396 اجرا شد. تیمارهای آزمایش شامل تنش خشکی در سه سطح (40، 80 و 120 میلی‌متر تبخیر از تشتک تبخیر کلاس A) در کرت‌های اصلی، محلول‌پاشی پوتریسین در سه سطح (Put0: صفر، Put1: 0.5 و Put2: 2 میلی‌مولار) و محلول‌پاشی 24-اپی‌براسینولید در سه سطح (Br0: صفر، Br1: 0.5 و Br2: 2 میکرو‌مولار) به‌صورت فاکتوریل در کرت‌های فرعی اجرا گردید. نتایج نشان داد که برهم‌کنش اثرهای چندگانه سال، تنش خشکی، پوتریسین و 24-اپی‌براسینولید تأثیر معنی‌داری بر صفات کلروفیلa، کلروفیلb و درصد اسانس ریحان گذاشت؛ به‌طوری‌که در هر دو سال آزمایش، محلول‌پاشی با 2 میلی‌مولار پوترسین و 2 میکرومولار 24-اپی‌براسینولید در همه سطوح تنش خشکی به‌ویژه 80 میلی‌متر تبخیر از تشتک تبخیر، سبب افزایش بیشتر این صفات شد و این افزایش در سال اول بیشتر از سال دوم بود. تیمار آبیاری مطلوب (40 میلی‌متر تبخیر از تشتک تبخیر) همراه با کاربرد غلظت 2 میلی‌مولار پوترسین و 0.5 میکرومولار 24-اپی‌براسینولید و تیمارهای تنش خشکی (80 و 120 میلی‌متر تبخیر از تشتک تبخیر) همراه با کاربرد غلظت2 میلی‌مولار پوترسین و با 2 میکرومولار 24-اپی‌براسینولید تعداد برگ و شاخه‌ فرعی بیشتری را داشتند. نتایج این پژوهش نشان داد که تنش خشکی سهم مهمی بر کاهش فاکتورهای اساسی در رشد ریحان دارد. به طور کلی، طبق نتایج به‌دست آمده، مصرف غلظت 2 میلی‌مولار پوتریسین همراه با 2 میکرومولار 24-اپی‌براسینولید برای بهبود فعالیت‌های فیزیولوژیکی و افزایش میزان اسانس و کاربرد توأم 2 میلی‌مولار پوتریسین و 0.5 میکرومولار 24-اپی‌براسینولید جهت افزایش عملکرد دانه ریحان در شرایط تنش خشکی پیشنهاد می‌شود.

کلیدواژه‌ها

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