نوع مقاله : مقاله پژوهشی
نویسندگان
1 فارغ‌التحصیل کارشناسی ارشد گیاهان دارویی، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه ایلام، ایلام، ایران
2 دانشیار، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه ایلام، ایلام، ایران
3 استادیار، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه ایلام، ایلام، ایران
چکیده
شناسه دیجیتال (DOR):
98.1000/1735-0905.1399.36.1038.104.6.1575.41
به‌منظور بررسی تأثیر محلول‌پاشی اسید جیبرلیک و اسید سالیسیلیک بر شاخص‌های مورفوفیزیولوژیکی، بیوشیمیایی و عملکرد گیاه دارویی سرخارگل Echinacea purpurea (L.) Moench این پژوهش طی سال‌های 1397 و 1398 به‌صورت آزمایش فاکتوریل بر پایه طرح بلوک‌های کامل تصادفی با سه تکرار در مزرعه تحقیقاتی دانشکده کشاورزی دانشگاه ایلام اجرا شد. تیمارهای آزمایش شامل غلظت‌های صفر، 100 و ppm200 اسید جیبرلیک و اسید سالیسیلیک و همچنین ترکیب آنها بود که به‌صورت محلول‌پاشی طی دوره رشد رویشی روی گیاه سرخارگل طی دو سال زراعی مورد مطالعه قرار گرفت. نتایج نشان داد که تیمارها در هر دو سال بررسی باعث بهبود شاخص‌های مورد مطالعه در گیاه سرخارگل شدند، به‌طوری که بیشترین ارتفاع بوته، تعداد گل، وزن تر و خشک بوته در سال اول و دوم مربوط به تیمار اسید جیبرلیک ppm200 و بیشترین میزان کلروفیل کل و کاروتنوئید در هر دو سال بررسی مربوط به اسید جیبرلیک ppm100 بود. همچنین بیشترین قطر کاپیتول و وزن تر و خشک گل در سال اول و بیشترین محتوای آنتوسیانین در سال دوم مربوط به تیمار اسید جیبرلیک ppm200 بود. از سویی بیشترین قطر کاپیتول در سال دوم و بیشترین میزان آنتوسیانین سال اول نیز مربوط به اسید سالیسیلیک ppm100 بود. علاوه‌براین بیشترین وزن تر و خشک گل در سال دوم نیز مربوط به تیمار اسید سالیسیلیک ppm200 بود. بیشترین میزان اسانس گل، اسانس شاخساره و اسانس کل در سال اول و دوم در ترکیب اسید جیبرلیک ppm100 و اسید سالیسیلیک ppm100 بدست آمد. با توجه به اینکه افزایش عملکرد وزن خشک بوته و همچنین میزان اسانس از مهمترین اهداف این پژوهش هستند، برترین تیمار پیشنهادی در این پژوهش برای افزایش وزن خشک بوته تیمار اسید جیبرلیک ppm200 و برای افزایش میزان اسانس ترکیب اسید جیبرلیک ppm100 و اسید سالیسیلیک ppm100 است.
کلیدواژه‌ها
موضوعات
- Abbaspour, H. and Rezaei, H., 2014. Effects of gibberellic effects of gibberellic acid on hill reaction, photosynthetic pigment and phenolic compounds in Moldavian dragonhead (Dracocephalum moldavica L.) in different drought stress levels. Plant Researches (Iranian Journal of Biology), 27(5): 893-903.
- Akter, A., Ali, E., Islam, M.M.Z., Karim, R. and Razzaque, A.H.M., 2007. Effect of GA3 on growth and yield of mustard. International Journal of Sustainable Crop Production, 2(2): 16-20.
- Ashraf, M. and Foolad, M.R., 2005. Pre-sowing seed treatment-A shotgun approach to improve germination, plant growth, and crop yield under saline and non-saline conditions. Advances in Agronomy, 88: 223-271.
- Bais, H.P., Sudha, G., George, J. and Ravishankar, G.A., 2001. Influence of exogenous hormones on growth and secondary metabolite production in hairy root cultures of Cichorium intybus L. cv. Lucknow local. In Vitro Cellular and Developmental Biology-Plant, 37(2): 293-299.
- Barnes, J., Anderson, L.A., Gibbons, S. and Phillipson, J.D., 2005. Echinacea species (Echinacea angustifolia (DC.) Hell., Echinacea pallida (Nutt.) Nutt., Echinacea purpurea (L.) Moench): a review of their chemistry, pharmacology and clinical properties. Journal of Pharmacy and Pharmacology, 57(8): 929-954.
- Barrett, B., 2003. Medicinal properties of Echinacea: a critical review. Phytomedicine, 10(1): 66-86.
- Burt, S., 2004. Essential oils: their antibacterial properties and potential applications in foods-a review. International Journal of Food Microbiology, 94(3): 223-253.
- Chehrazi, M., Hosseini, H.R., Hashemi, D.E. and Asadi, V.K., 2017. The effects of gibberellic acid on some morpho-physiological characteristics of two varieties of white and yellow flowers (Alba and Apollo) Snapdragon (Antirrhinum majus). Iranian Journal of Horticultural Science, 48(2): 265-273.
- Cong, H.A.N., Zuo, J.H., Qing, W.A.N.G., Dong, H.Z. and Gao, L.P., 2017. Salicylic acid alleviates postharvest chilling injury of sponge gourd (Luffa cylindrica). Journal of Integrative Agriculture, 16(3): 735-741.
- El-Aal, F.S.A., Shaheen, A.M. and Rizk, F.A., 2008. The effect of foliar application of GA3 and soil dressing of NPK at different levels on the plant productivity of Potatoes (Solanum tuberosum L.). Research Journal of Agriculture and Biological Sciences, 4(5): 384-391.
- Farsaraei, S., Moghaddam, M. and Mehdizadeh, L., 2019. Effect of salicylic acid on growth and biochemical characteristics and essential oil percentage of Lemon verbena at different irrigation water salinity. Journal of Water Research in Agriculture (Soil and Water Science), 33(1): 95-107.
- Gerami, M., Akbarpour, V. and Mohammadian, A., 2019. The effect of putrescine and salicylic acid on physiological characteristics and antioxidant in Stevia rebaudiana B. under salinity stress. Journal of Crop Breeding, 11(29): 40-54.
- Gharib, F.A., 2006. Effect of salicylic acid on the growth, metabolic activities and oil content of basil and marjoram. International Journal of Agriculture and Biology, 4: 485-492.
- Ghatas, Y.A.A. and Abdallah, W. H., 2016. Effect of Some Fertilization and Micro-Nutrients Treatments on Growth and Chemical Constituents of Echinacea purpurea plant. Journal of Plant Production, 7(7): 709-719.
- Góraj, J., Węgrzynowicz-Lesiak, E. and Saniewski, M., 2014. The effect of some plant growth regulators and their combination with methyl jasmonate on anthocyanin formation in roots of Kalanchoe blossfeldiana. Journal of Horticultural Research, 22(2): 31-40.
- Hajisamadi, A.B., Hassanpouraghdam, M.B. and Khalighi, A., 2011. Effects of gibberellic acid (GA3) foliar application on growth characteristics and essential oil of Lavender (Lavandula officinalis Chaix.). Journal of Agricultural Science and Sustainable Production, 21: 23-32.
- Hassanpouraghdam, M.B., Asl, B.H. and Khalighi, A., 2011. Gibberellic acid foliar application influences growth, volatile oil and some physiological characteristics of lavender (Lavandula officinalis Chaix.). Romanian Biotechnological Letters, 16(4): 6322-6327.
- Hou, K., Li, J.Y., Chen, J.W., Shen, H., Wu, W. and Chen, L., 2018. Effect of gibberellic acid and chlormequat chloride on growth, coumarin content and root yield of Angelica dahurica var. formosana. Journal of Agricultural Science and Technology, 15(7): 1415-1423.
- Jini, D. and Joseph, B., 2017. Physiological mechanism of salicylic acid for alleviation of salt stress in rice. Rice Science, 24(2): 97-108.
- Jones, A.M.P., Saxena, P.K. and Murch, S.J., 2009. Elicitation of secondary metabolism in Echinacea purpurea L. by gibberellic acid and triazoles. Engineering in Life Sciences, 9(3): 205-210.
- Khavarinezhad, R.A., Mehrabian, S. and Asadi, A., 2004. The effect of salicylic acid on the concentration of anthocyanins in infected Daisy (Bellis perennis L.) plants by some of the fungi lines. Journal of Science (Kharazmi University), 4(3): 427-438.
- Kim, J.S., Lee, B.H., Kim, S.H., Oh, K.H. and Cho, K.Y., 2006. Responses to environmental and chemical signals for anthocyanin biosynthesis in non-chlorophyllous Corn (Zea mays L.) leaf. Journal of Plant Biology, 49(1): 16-25.
- Latunde-Dada, A.O. and Lucas, J.A., 2001. The plant defence activator acibenzolar-S-methyl primes cowpea [Vigna unguiculata (L.) Walp.] seedlings for rapid induction of resistance. Physiological and Molecular Plant Pathology, 58(5): 199-208.
- Liang, Z., Ma, Y., Xu, T., Cui, B., Liu, Y., Guo, Z. and Yang, D., 2013. Effects of abscisic acid, gibberellin, ethylene and their interactions on production of phenolic acids in Salvia miltiorrhiza Bunge hairy roots. Plos One, 8(9): e72806.
- Lichtenthaler, H.K. and Wellburn, A.R., 1983. Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochemical Society. Transaction, 11: 591-592.
- Malekian, M., Hemmati, K., Ghasemnezhad, A. and Barzali, M., 2014. Effect of salicylic acid on quantitative and qualitative traits of Matricaria chamomilla ecotypes. Journal of Crops Improvement, 16(1): 185-196.
- Masukasu, H., Karin, O. and Kyoto, H., 2003. Enhancement of anthocyanin biosynthesis by sugar in radish (Raphanus sativus) hypocotyls. Plant Science, 164(2): 259-265.
- Mehalaine, S., Menasria, T., Bouguessa, S. and Yahia, A., 2017. In vitro seed germination of some Algerian medicinal plants and the effect of gibberellic acid (GA3) on breaking dormancy. Journal of Materials and Environmental Sciences, 8(6): 2034-2039.
- Moein, P. and Mortezaeinejad, F., 2014. Investigation of the hormonal effects of gibberellic acid, salicylic acid and kinetin on morphological and reproductive traits of agricultural Saffron flowers (Crocus sativus L.). The 1st International Conference on New Ideas in Agriculture Islamic Azad University Khorasgan Branch, 25-26 January, Isfahan, Iran.
- Mostafa, H.A.M., El-Bassiouny, M.S., Khattab, K.I. and Sadak, M.S., 2005. Improving the characteristics of roselle seeds as a new source of protein and lipid by gibberellin and benzyladenine application. Journal of Applied Science Research, 2: 161-167.
- Mozafari, V. and Khaleghi, F., 2016. Effects of gibberellic acid and nitrogen on some physiology parameters and micronutrients concentration in Pistachio under salt stress. Journal of Water and Soil, 30(3): 955-967.
- Nazarbeigi, E., Fallahi, H.A., Naseri, R., Mirzaei, A. amd Rashidpour, M., 2014. Effect of different concentrations of salt (NaCl), salicylic acid (SA) and gibberellic acid (GA3) on proline content and a, b chlorophylls of two cultivars of Canola (Hayola 401 & RGS). Journal of Applied Research of Plant Ecophysiology, 1(2): 25-37.
- Nyalambisa, M., Oyemitan, I.A., Matewu, R., Oyedeji, O.O., Oluwafemi, O.S., Songca, S.P., Nkeh-Chungag, B.N. and Oyedeji, A.O., 2017. Volatile constituents and biological activities of the leaf and root of Echinacea species from South Africa. Saudi Pharmaceutical Journal, 25(3): 381-386.
- Pazoki, A. R., Rezaei, H., Habibi, D. and Paknejad, F., 2012. Effect of drought stress, asscorbate and gibberellin foliar application on some morphological traits, RWC and cell membrane stability of Thyme (Thymus vulgaris L.). Journal of Agronomy and Plant Breeding, 8(1): 1-13.
- Porra, R.J., 2002. The chequered history of the development and use of simultaneous equations for the accurate determination of chlorophylls a and b. Photosynthesis Research, 73(1-3): 149-156.
- Pramanik, K., Adhikari, A., Bera, A.K. and Mandal, B., 2015. Effect of seed priming and mulching on growth and productivity of rain-fed Sesame (Sesamum Indicum L.) during summer season. International Journal of Bioresource Science, 2(1): 25-31.
- Ramawat, K.G., Dass, S. and Mathur, M., 2009. The chemical diversity of bioactive molecules and therapeutic potential of medicinal plants: 7-32. In: Ramawat, K.G., (Ed.). Herbal Drugs: Ethnomedicine to Modern Medicine. Springer, Berlin, Heidelberg, 402p
- Ramroodi, M. and Khamar, A., 2013. Interaction effects of salicylic acid spraying and irrigation treatments on some quantitative, qualitative and osmotic regulators of Basil. Applied Research of Plant Ecophysiology Journal, 1(1): 19-32.
- Sajid, M., Amin, N., Ahmad, H. and Khan, K., 2016. Effect of gibberellic acid on enhancing flowering time in Chrysanthemum morifolium. Pakistan Journal of Botany, 48(2): 477-483.
- Sardoei, A.S. and Shahdadneghad, M., 2014. Effects of foliar application of gibberellic acid on chlorophyll and carotenoids of Marigold (Calendula officinalis L.). International Journal of Advanced Biological and Biomedical Research, 2(6): 1887-1893.
- Sedghi, M., Sheikhnavaz Jahed, P. and Seyed Sharifi, R., 2018. Effect of gibberellic acid and priming on germination and some constituents of Stevia (Stevia rebaudiana Bertoni). Journal of Plant Process and Function, 7(26): 199-208.
- Seo, S., Ishizuka, K. and Ohashi, Y., 1995. Induction of salicylic acid β-glucosidase in tobacco leaves by exogenous salicylic acid. Plant and Cell Physiology, 36(3): 447-453.
- Shakeri, M., Aminifard, M.H., Behdani, M.A. and Tabatabaei, S.J., 2018. Study of the effect hormone of gibberellic acid and corm weight on vegetative and yield traits of Saffron (Crocus sativus L.). Journal of Plant Production Research, 25 (2): 153-165.
- Sharifuzzaman, S.M., Ara, K.A., Rahman, M.H., Kabir, K. and Talukdar, M.B., 2011. Effect of GA3, CCC and MH on vegetative growth, flower yield and quality of chrysanthemum. International Journal of Experimental Agriculture 2(1): 17-20.
- Shirzad, S.U.R.E., Arooie, H. and Azizi, M., 2012. Influence of plant growth regulators (PGRs) and planting method on growth and yield in oil Pumpkin (Cucurbita pepo var. styriaca). Notulae Scientia Biologicae, 4(2): 101-107.
- Shohani, F., Mehrabi, A.A., Khavarinegad, R.A., Safari, Z. and Kian, S., 2014. The effect of gibberellic acid (GA3) on seed germination and early growth of Lentil seedlings under salinity stress. Middle East Journal of Scientific Research, 19(7): 995-1000.
- Sifola, M.I. and Barbieri, G., 2006. Growth, yield and essential oil content of three cultivars of basil grown under different levels of nitrogen in the field. Scientia Horticulturae, 108(4): 408-413.
- Stewart, A.J., Chapman, W., Jenkins, G.I., Graham, I., Martin, T. and Crozier, A., 2001. The effect of nitrogen and phosphorus deficiency on flavonol accumulation in plant tissues. Plant, Cell and Environment, 24(11): 1189-1197.
- Wist, T.J. and Davis, A.R., 2006. Floral nectar production and nectary anatomy and ultrastructure of Echinacea purpurea (Asteraceae). Annals of Botany, 97(2): 177-193.
- Yazdani, B.R., Bannayan, A.M., Khazaei, H.R. and Sodaeeizadeh, H., 2014. Investigating the effects of urea, azocompost and cutting on quantitative and qualitative characteristics of Oregano (Origanum vulgare Virid). Journal of Agricultural Ecology, 6(4): 798-811.
- Yousef, R.M., Khalil, S.E. and El-Said, N.A., 2013. Response of Echinacea purpurea L. to irrigation water regime and bio-fertilization in sandy soils. World Applied Sciences Journal, 26(6): 771-782.
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