همکاری با انجمن علمی گیاهان دارویی ایران

نوع مقاله : مقاله پژوهشی

نویسندگان

1 فارغ‌التحصیل کارشناسی ارشد بیوتکنولوژی کشاورزی، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران، ایران

2 دانشیار، گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران، ایران

3 استادیار، گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران، ایران

چکیده

در این پژوهش، ریشه‌های مویین گیاه سرخارگل (Echinacea purpurea L.) در بیوراکتور ستون حباب‌دار 1000 میلی‌لیتری حاوی 500 میلی‌لیتر محیط کشت WPM مایع کشت و تأثیر مقادیر اینوکلوم (3، 6 و gl-19) و سرعت‌های هوادهی (1/0، 2/0 و vvm4/0) روی میزان تولید بیوماس و اسید شیکوریک بررسی شدند. صفات وزن تر، وزن خشک و مقدار اسید شیکوریک ریشه‌های مویین بعد از 30 روز اندازه‌گیری شدند. نتایج نشان داد که بیشترین مقدار بیوماس (g l-14/16 وزن تر و g l-1084/2 وزن خشک) و اسید شیکوریک (mg g-1 DW74/16) در مقدار اینوکلوم g l-1 6 تولید شده است. نتایج حاصل از بررسی اثر سرعت هوادهی بر تولید بیوماس و اسید شیکوریک نیز نشان داد که بیشترین میزان تولید وزن تر ( g l-14/15) و وزن خشک ( g l-1467/2) در سرعت هوادهی vvm 4/0 و بیشترین میزان تولید اسید شیکوریک (mg g-1 DW 74/12) در سرعت هوادهی vvm 2/0 بدست آمده است. به‌طور کلی مقدار اینوکلوم و سرعت هوادهی نقش مهمی در میزان رشد و نمو ریشه‌های مویین در بیوراکتور ستون حباب‌دار داشتند و برای بهینه کردن تولید بیوماس و متابولیت ثانویه باید به این عوامل توجه کرد.

کلیدواژه‌ها

- Abbasi, B.H., Liu, R., Saxena, P. and Liu, C.Z., 2009. Cichoric acid production from hairy root cultures of Echinacea purpurea grown in a modified airlift bioreactor. Journal of Chemical Technology and Biotechnology, 84(11): 1697-1701.
- Abbasi, B.H., Stiles, A.R., Saxena, P.K. and Liu, C.Z., 2012. Gibberellic acid increases secondary metabolite production in Echinacea purpurea hairy roots. Applied Biochemistry and Biotechnology, 168(7): 2057-2066.
- Abbasi, B.H., Tian, C.L., Murch, S.J., Saxena, P.K. and Liu, C.Z., 2007. Light-enhanced caffeic acid derivatives biosynthesis in hairy root cultures of Echinacea purpurea. Plant Cell Reports, 26(8): 1367-1372.
- Abdoli, M., 2013. Study on Cichoric acid production via optimization of hairy roots culture and polyploidy induction in purple coneflower (Echinacea purpurea L.).Ph.D thesis, Department of Plant Breeding and Biotechnology, Facultuy of Agriculture, Tarbiat Modares University, Iran.
- Abdoli, M., Moieni A. and Naghdi Badi, H., 2013. Influence of KNO3, CaCl2 and MgSO4 concentrations on growth and cichoric acid accumulation in hairy root culture of purple coneflower (Echinacea purpurea L.). Journal of Medicinal Plants, 45: 75-84.
- Bakkali, A.T., Jaziri, M., Foriers, A., Vander Heyden, Y., Vanhaelen, M. and Homes, J. 1997. Lawsone accumulation in normal and transformed cultures of henna, Lawsonia inermis. Plant Cell, Tissue and Organ Culture, 51(2): 83-87.
- Banerjee, S., Rahman, L., Uniyal, G.C. and Ahuja, P.S., 1998. Enhanced production of valepotriates by Agrobacterium rhizogenes induced hairy root cultures of Valeriana wallichii DC. Plant Science, 131(2): 203-208.
- Baque, M.A., Moh, S.H., Lee, E.J., Zhong, J.J. and Paek, K.Y. 2012. Production of biomass and useful compounds from adventitious roots of high-value added medicinal plants using bioreactor. Biotechnology Advances, 30(6): 1255-1267.
- Bourgaud, F., Gravot, A., Milesi, S. and Gontier, E., 2001. Production of plant secondary metabolites: a historical perspective. Plant Science, 161(5): 839-851.
- Chattopadhyay, S., Farkya, S., Srivastava, A.K. and Bisaria, V.S., 2002. Bioprocess considerations for production of secondary metabolites by plant cell suspension cultures. Biotechnology and Bioprocess Engineering, 7: 138-149.
- Choi, Y.E., Kim, Y.S. and Paek, K.Y., 2006. Types and designs of bioreactors for hairy root culture. Plant Tissue Culture Engineering, 6: 161-172.
- Cui, X.H., Chakrabarty, D., Lee, E.J. and Paek, K.Y., 2010. Production of adventitious roots and secondary metabolites by Hypericum perforatum L. in a bioreactor. Bioresource Technology, 101(12): 4708-4716.
- Curtis, W.R., 2000. Hairy roots, bioreactor growth: 827-841. Spier, R.E., (Ed.). Encyclopedia of Cell Technology. Wiley-Interscience, 1249p.
- Eibl, R. and Eibl, D., 2008. Design and use of the wave bioreactor for plant cell culture. Plant Tissue Culture Engineering, 6: 203-227.
- Huang, S.Y., Hung, C.H. and Chou, S.N., 2004. Innovative strategies for operation of mist trickling reactors for enhanced hairy root proliferation and secondary metabolite productivity. Enzyme and Microbial Technology, 35: 22-32.
- Huang, T.K. and McDonald, K.A., 2012. Bioreactor systems for in vitro production of foreign proteins using plant cell cultures. Biotechnology Advances, 30(2): 398-409.
- Jeong, J.A., Wu, C.H., Murthy, H.N., Hahn, E.J. and Paek, K.Y., 2009. Application of an airlift bioreactor system for the production of adventitious root biomass and caffeic acid derivatives of Echinacea purpurea. Biotechnology and Bioprocess Engineering, 14: 91-98.
- Kayser, O. and Quax, W.J., 2007. Medicinal Plant Biotechnology: From Basic Research to Industrial Applications. Weinheim, Wiley-VCH Press, 576p.
- Kino-Oka, M., Hitaka, Y., Taya, M. and Tone, S., 1999. High-density culture of red beet hairy roots by considering medium flow condition in a bioreactor. Chemical Engineering Science, 54(15-16): 3179-3186.
- Kittipongpatana, N., Hock, R.S. and Porter, J.R., 1998. Production of solasodine by hairy root, callus, and cell suspension cultures of Solanum aviculare Forst. Plant Cell, Tissue and Organ Culture, 52: 133-143.
- Kwok, K.H. and Doran, P.M., 1995. Kinetic and stoichiometric analysis of hairy roots in a segmented bubble column reactor. Biotechnology Progress, 11(4): 429-435.
- Lee, C.W. and Shuler, M.L., 2000. The effect of inoculum density and conditioned medium on the production of ajmalicine and catharanthine from immobilized Catharanthus roseus cells. Biotechnology and Bioengineering, 67: 61-71.
- Lee, J. and Scagel, C.F., 2013. Chicoric acid: chemistry, distribution, and production. Frontiers in Chemistry, 1: 1-17.
- Liu, C.Z., Wang, Y., Zhao, B., Guo, C., Ouyang, F., Ye, H. and Li, G., 1999. Development of a nutrient mist bioreactor for growth of hairy roots. In Vitro Cellular & Developmental Biology Plant, 35: 271-274.
- Liu, R., Li, W., Sun, L. and Liu, C., 2012. Improving root growth and cichoric acid derivatives production in hairy root culture of Echinacea purpurea by ultrasound treatment. Biochemical Engineering Journal, 60: 62-66.
- Liu. C.Z., Abbasi, B.H., Gao, M., Murch, S.J. and Saxena, P.K., 2006. Caffeic acid derivatives production by hairy root cultures of Echinacea purpurea. Journal of Agricultural and Food Chemistry, 54(22): 8456-8460.
- Min, J.Y., Jung, H.Y., Kang, S.M., Kim, Y.D., Kang, Y.M., Park, D.J., Prasad, D.T. and Choi, M.S., 2007. Production of tropane alkaloids by small-scale bubble column bioreactor cultures of Scopolia parviflora adventitious roots. Bioresource Technology, 98: 1748-1753.
- Murthy, H.N., Lee, E.J. and Paek, K.Y., 2014. Production of secondary metabolites from cell and organ cultures: strategies and approaches for biomass improvement and metabolite accumulation. Plant Cell, Tissue and Organ Culture (PCTOC): 1-16.
- Paek, K., Chakrabarty, D. and Hahn, E., 2005. Application of bioreactor systems for large scale production of horticultural and medicinal plants. Plant Cell, Tissue and Organ Culture, 81(3): 287-300.
- Ramakrishnan, D., Salim, J. and Curtis, W.R., 1994. Inoculation and tissue distribution in pilot-scale plant root culture bioreactors. Biotechnology Techniques, 8(9): 639-644.
- Romero, F.R., Delate, K., Kraus, G.A., Solco, A.K., Murphy, P.A. and Hannapel, D.J., 2009. Alkamide production from hairy root cultures of Echinacea. In Vitro Cellular & Developmental Biology-Plant, 45: 599-609.
- Schlernitzauer, A., Oiry, C., Hamad, R., Galas, S., Cortade, F., Chabi, B., Casas, F., Pessemesse, L., Fouret, G. and Feillet-Coudraym, C., 2013. Chicoric acid is an antioxidant molecule that stimulates AMP Kinase pathway in L6 Myotubes and extends lifespan in Caenorhabditis elegans. Plos One, 8: 78-88.
- Sivakumar, G., 2006. Bioreactor technology: A novel industrial tool for high-tech production of bioactive molecules and biopharmaceuticals from plant roots. Biotechnology Journal, 1: 1419-1427.
- Wang, B., Zhang, G., Zhu, L., Chen, L. and Zhang, Y., 2006. Genetic transformation of Echinacea purpurea with Agrobacterium rhizogenes and bioactive ingredient analysis in transformed cultures. Colloids and Surfaces Biointerfaces, 53: 101-104.
- Weathers, P., Wyslouzil, B., Wobbe, K., Kim, Y. and Yigit, E., 1999. The biological response of hairy roots to O2 levels in bioreactors. In Vitro Cellular & Developmental Biology-Plant, 35: 286-289.
- Yesil Celiktas, O., Gurel, A. and Vardar-Sukan, F., 2010. Large scale cultivation of plant cell and tissue culture in bioreactors. Transworld Research Network, 1-54.