نوع مقاله : مقاله پژوهشی
نویسنده
استادیار، گروه علوم دامی، مجتمع آموزش عالی تربت‌جام، تربت‌جام، ایران
چکیده
شناسه دیجیتال (DOR):
98.1000/1735-0905.1398.35.902.98.6.1576.1610
درمنه کوهی (.Artemisia aucheri Boiss) گیاهی دارویی متعلق به خانواده Asteraceae بوده که اثرهای ضد میکروبی آن به فراوانی بررسی شده است. اطلاعات محدودی در مورد اثر عصاره هگزانی این گیاه بر شرایط تخمیر ایجاد شده توسط میکروارگانیسم‌های شکمبه‌ای (باکتری‌ها، قارچ‌ها و پروتوزوآ) وجود دارد. ازاین‌رو، این پژوهش با هدف بررسی عصاره هگزانی استخراج شده از درمنه کوهی با سوکسله (در چهار سطح صفر (شاهد)، 150، 300 و 450 میلی‌گرم/لیتر) بر ویژگی‌های تخمیری، فراسنجه‌های تولید گاز و قابلیت هضم آزمایشگاهی یک جیره تهیه شده برای بره‌های پرواری در شرایط آزمایشگاهی انجام شد. با افزایش سطح عصاره به محیط کشت، pH و اسیدهای چرب فرّار کل (TVFA) نسبت به تیمار شاهد (به‌صورت خطی) به‌ترتیب کاهش و افزایش یافتند. بیشترین مقدار فراسنجه‌های تولید گاز (قابلیت تولید گاز، تولید تجمعی گاز در زمان‌های 48 و 72 ساعت) و کمترین مقدار ثابت میزان تولید گاز (cgas)، ضریب تفکیک‌پذیری و بازده سنتز توده میکروبی نیز در سطح 450 میلی‌گرم/لیتر عصاره مشاهده شد. با توجه به نتایج موجود، به‌نظر می‌رسد که عصاره هگزانی درمنه کوهی تا حدودی توانسته است شرایط تخمیر را در محیط کشت به‌ویژه با افزایش TVFA به‌عنوان مهمترین منبع تولید انرژی در نشخوارکنندگان بهبود بخشد.
کلیدواژه‌ها
- AOAC., 1999. Official Methods of Analysis. Association of Official Analytical Chemists, Washington, DC, USA.
- Baratta, M.T., Damien Dorman, H.J., Deans, S.G., Figueiredo, A.C., Barroso, J.G. and Ruberto, G., 1998. Antimicrobial and antioxidant properties of some commercial essential oils. Flavour and Fragrance Journal, 13: 235-244.
- Barnett, A.J.G. and Reid, R., 1957. Studies on the production of volatile fatty acids from grass in artificial rumen. 1. Volatile fatty acids production from fresh grasses. The Journal of Agricultural Science, 48: 315-321.
- Benchaar, C., Chaves, A.V., Fraser, G.R., Wang, Y., Beauchemin K.A. and McAllister, T.A., 2007. Effects of essential oils and their components on in vitro rumen microbial fermentation. Canadian Journal of Animal Science, 87(3): 413-419.
- Blümmel, M., Steingass, H. and Becker, K., 1997. The relationship between in vitro gas production, in vitro microbial biomass yield and 15N incorporation and its implications for prediction of voluntary feed intake of roughages. British Journal of Nutrition, 77(6): 911-921.
- Burt, S., 2004. Essential oils: their antibacterial properties and potential applications in foods: a review. International Journal of Food Microbiology, 94(3): 223-253.
- Calsamiglia, S., Busquet, M., Cardozo, P.W., Castillejos, L. and Ferret, A., 2007. Invited review: essential oils as modifiers of rumen microbial fermentation. Journal of Dairy Science, 90(6): 2580-2595.
- Calsamiglia, S., Castillejos, L. and Busquet, M., 2006. Alternatives to antimicrobial growth promoters in cattle: 129-167. In: Garnsworthy, P.C. and Wiseman, J., (Eds.). Recent Advances in Animal Nutrition. Notthingham University Press, Nottingham, UK, 388p.
- Cardozo, P.W., Calsamiglia, S., Ferret, A. and Kamel, C., 2005. Screening for the effects of natural plant extracts at two pH level on in vitro rumen microbial fermentation of a high-concentrate diet for beef cattle. Journal of Animal Science, 83: 2572-2579.
- Castillejos, L., Calsamiglia, S., Ferret, A. and Losa, R., 2005. Effects of a specific blend of essential oil compounds and the type of diet of rumen microbial fermentation and nutrient flow from continuous culture systems. Animal Feed Science and Technology, 119: 29-41.
- Castillejos, L., Calsamiglia, S. and Ferret, A., 2006. Effect of essential oils active compounds on rumen microbial fermentation and nutrient flow in in vitro systems. Journal of Dairy Science, 89: 2649-2658.
- Castillejos, L., Calsamiglia, S., Ferret, A. and Losa, R., 2007. Effects of dose and adaptation time of a specific blendof essential oil compound on rumen fermentation. Animal Feed Science and Technology, 132: 186-201.
- Castillejos, L., Calsamiglia, S., Martın-Tereso, J. and Ter Wijlen, H., 2008. In vitro evaluation of effects of ten essential oils at three doses on ruminal fermentation of high concentrate feedlot-type diets. Animal Feed Science and Technolology, 145: 259-270.
- Cobellis, G., Trabalza-Marinucci, M. and Zhongtang, Y., 2016. Critical evaluation of essential oils as rumen modifiers in ruminant nutrition: A review. Science of the Total Environment, 545-546: 556-568.
- Dinani, N.J., Asgary, A., Madani, H., Naderi, G. and Mahzoni, P., 2010. Hypocholesterolemic and antiatherosclerotic effect of Artemisia aucheri in essential oils of three species of Artemisia on some soil-borne phytopathogens. Communications in Agricultural and Applied Biological Sciences, 71: 1327-1333.
- Doroodgar, A., Arbabi, M., Razavi, M.R., Mohebali, M., Sadr, F. and Tashakkor, Z., 2007. Effect of Artemisia sieberi extract on Leishmania major ulcers in BALB/c mice. Feyz (Journal of Kashan University of Medicinal Science), 11(3): 52-56.
- Feldberg, R.S., Chang, S.C., Kotik, A.N., Nadler, M., Neuwirth, Z., Sundstrom, D.C. and Thompson, N.H., 1988. In vitro mechanism of inhibition of bacterial cell growth by allicin. Antimicrobial Agents Chemistry, 32: 1763-1768.
- Fraser, G.R., Chaves, A.V., Wang, Y., McAllister, T.A., Beauchemin, K.A. and Benchaar, C., 2007. Assessment of the effects of Cinnamon leaf oil on rumen microbial fermentation using two continuous culture systems. Journal of Dairy Science, 90(5): 2315-2328.
- Getachew, G., Robinson, P.H., DePeters, E.J. and Taylor, S.J., 2004. Relationships between chemical composition, dry matter degradation and in vitro gas production of several ruminant feeds. Animal Feed Science and Technology, 111(1-4): 57-71.
- Giordani, R., Regli, P., Kaloustian, J., Mikaıl, C., Abou, L. and Portugal, H., 2004. Antifungal effect of various essential oils against Candida albicans. Potentiation of antifungal action of Amphotericin B by essential oil from Thymus vulgaris. Phytotherapy Research, 18: 990-995.
- Hart, K.J., Yanez-Ruiz, D.R., Duval, S.M., McEwan, N.R. and Newbold, C.J., 2008. Plant extracts to manipulate rumen fermentation. Animal Feed Science and Technology, 147: 8-35.
- JimEnez-Peralta, F.S., Salem, A.Z.M., Mejia-Hernndez, P., Gonzlez-Ronquillo, M., lbarrn-Portillo, B., Rojo-Rubio, R. and Tinoco-Jaramillo, J.L., 2011. Influence of individual and mixed extracts of two tree species on in vitro gas production kinetics of a high concentrate diet fed to growing lambs. Livestock Science, 136: 192-200.
- Kazemi, M., Tahmasbi, A.M., Valizadeh, R., Naserian, A.A. and Moheghi, M.M., 2009. Assessment of nutritive value of four dominant weed species in range of Khorasan distinct of Iran by in vitro and in situ techniques. Journal of Animal and Veterinary Advances, 8(11): 2286-2290.
- Komolong, M.K., Barber, D.G. and McNeill, D.M., 2001. Post-ruminal protein supply and N retention of weaner sheep fed on a basal diet of lucerne hay (Medicago sativa) with increasing levels of quebracho tannins. Animal Feed Science and Technology, 92(1-2): 59-72.
- Makkar, H.P.S., 2010. In vitro screening of feed resources for efficiency of microbial protein synthesis: 107-144. In: Vercoe, P.E., Makkar, H.P.S. and Schlink, A.C., (Ed.). In Vitro Screening of Plant Resources for Extra-Nutritional Attributes in Ruminants: Nuclear and Related Methodologies. New York, Springer, 242p.
- Malecky, M., Broudiscou, L.P. and Schmidely, P., 2009. Effects of two levels of monoterpene blend on rumen fermentation, terpene and nutrient flows in the duodenum and milk production in dairy goats. Animal Feed Science and Technology, 154: 24-35.
- Massry, K., Ghorab, A. and Farouk, A., 2002. Antioxidant activity and volatile components of Egyptian, Artemisia judaica. Food Chemistry, 79: 331-336.
- McIntosh, F.M., Williams, P., Losa, R., Wallace, R.J., Beever, D.A. and Newbold, C.J., 2003. Effects of essential oils on ruminal microorganisms and their protein metabolism. Applied and Environmental Microbiology, 69: 5011-5014.
- Menke, K.H. and Steingass, H., 1988. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal Research Development, 28: 7-55.
- Nagy, J.G. and Tengerdy, R.P., 1968. Antibacterial action of essential oils of Artemisia as an ecological factor. II. Antibacterial action of the volatile oils of Artemisia tridentata (big sagebrush) on bacteria from the rumen of mule deer. Applied and Environmental Microbiology, 16: 441-444.
- Nasirpour, M., Yavarmanesh, M., Mohhamadi Sani, A., Nasirpour, M. and Mohamdzade Moghadam., M., 2014. Antibacterial effect of aqueous extract of Artemisia aucheri, Artemisia sieberi and Hyssopus officinalis L. on the food borne pathogenic bacteria. Food Science and Technology, 12(46): 73-84.
- Newbold, C.J., McIntosch, F.M.,Williams, P., Losa, R. and Wallace, R.J., 2004. Effects of a specific blend of essential oil compounds on rumen fermentation. Animal Feed Science and Technology, 114: 105-112.
- Nooriyan Soroor, E. and Rouzbehan, Y., 2014. The influence of Golpar (Heracleum persicum) on in vitro rumen fermentation parameter, sand on methane production. Iranian Journal of animal Science, 44(4): 385-395.
- NRC, 2007. Nutrient Requirements of Small Ruminants: Sheep, Goats, Cervids, and New World Camelids. Washington: National Academy Press, Washington, D.C., USA, 384p.
- Ørskov, E.R. and McDonald, I., 1979. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. Journal of Agricultural Science, 92: 499-503.
- Sallam, S.M.A., Bueno, I.C.S., Brigide, P., Godoy, P.B., Vittii, D.M.S.S. and Abdalla, A.L., 2009. Efficiency of Eucalyptus oil on in vitro ruminal fermentation and methane production. Nutritional and Foraging Ecology of Sheep and Goats, 85: 267-272.
- Santos, M.B., Robinson, P.H., Williams, P. and Losa, R., 2010. Effects of addition of an essential oil complex to the diet of lactating dairy cows on whole tract digestion of nutrients and productive performance. Animal Feed Science and Technology, 157(1-2): 64-71.
- SAS Institute INC., 2002. Sas user’s Guide: statistics. Statistical Analysis Systems Institute Inc. Cary NC.
- Sliwinski, B.J., SolivaCarla, R., Machmuller, A. and Kreuzer, M., 2002. Efficacy of plant extracts rich in secondary constituents to modify rumen fermentation. Animal Feed Science and Technology, 101: 101-114.
- Theodorou, M.K., Williams, B.A., Dhanoa, M.S., McAllan, A.B. and France, J., 1994. A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Animal Feed Science and Technology, 48: 185-197.
- Van Nevel, C.J. and Demeyer, D.I., 1988. Manipulation of rumen fermentation: 387-447. In: Hobson, P.N., (Ed.). The Rumen Microbial Ecosystem. Elsevier Applied Science, New York, USA, 708p.
- Vercoe, P.E., Makkar, H.P.S. and Schlink, A.C., 2010. In Vitro Screening of Plant Resources for Extra-Nutritional Attributes in Ruminants: Nuclear and Related Methodologies. New York, Springer, 242p.
- Wallace, R.J., Arthaud, L.C. and Newbold, J., 1994. Influence of Yucca schidigera extract on ruminal ammonia concentrations and ruminal microorganisms. Applied Environmental Microbiology, 60: 762-767.
- Wang, Y., McAllister, T.A., Newbold, C.J., Cheeke, P.R. and Cheng, K.J., 1997. Effects of Yucca extract on fermentation and degradation of saponins in the Rusitec. Proceedings of the Western Section, American Society of Animal Science. 48: 149-152.
- Yang, W.Z. Benchaar, C., Ametaj, B.N., Chaves, A.V., He, M.L. and McAllister, T.A., 2007. Effects of garlic and Juniper Berry essential oils on ruminal fermentation and on the site and extent of digestion in lactating cows. Journal of Dairy Science, 90(12): 5671-5681.
- Yang, W.Z., Ametaj, B.N. Benchaar, C. and Beauchemin, K.A., 2010. Dose response to cinnamaldehyde supplementation in growing beef heifers: Ruminal and intestinal digestion. Journal of Animal Science, 88(2): 680-688.
- Ye, D., Karnati, S.K.R., Wagner, B., Firkins, J.L., Eastridge, M.L. and Aldrich, J.M., 2018. Essential oil and monensin affect ruminal fermentation and the protozoal population in continuous culture. Journal of Dairy Science, 101: 1-13.
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