اثر محلول‌پاشی اسید جاسمونیک بر برخی صفات مورفوفیزیولوژیکی، فعالیت آنتی‌اکسیدانی و عملکرد اسانس مریم‌گلی (Salvia officinalis L.) تحت تنش خشکی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکترای بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل، زابل، ایران

2 دانشیار، گروه زراعت، دانشکده کشاورزی، دانشگاه زابل، زابل، ایران

3 استاد، گروه زراعت، دانشکده کشاورزی، دانشگاه زابل، زابل، ایران

4 دانشیار، گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل، زابل، ایران

چکیده

شناسه دیجیتال (DOR):
98.1000/1735-0905.1398.35.80.93.1.1575.1610

کمبود آب یکی از مهمترین عوامل محیطی محدودکننده رشد گیاهان و محصولات آنهاست. در این مطالعه، به‌منظور بررسی اثر اسید جاسمونیک بر کاهش صدمات ناشی از تنش خشکی روی شاخص‌های رشدی و برخی از پاسخ‌های فیزیولوژیکی و آنتی‌اکسیدانی گیاه دارویی مریم‌گلی (Salvia officinalis L.)، آزمایشی به‌صورت فاکتوریل در قالب طرح کاملاً تصادفی با ۳ تکرار در دانشگاه زابل اجرا شد. تیمارهای آزمایشی شامل تنش خشکی (آبیاری در حد ظرفیت زراعی، تخلیه 5۰% و ۷5% رطوبت ظرفیت زراعی) و محلول‌پاشی اسید جاسمونیک (صفر (شاهد)، 75 و 150 میلی‌گرم در لیتر) بودند. نتایج نشان داد که محلول‌پاشی با غلظت ۱5۰ مولار اسید جاسمونیک در شرایط تنش خشکی ارتفاع (10.02%)، وزن تر (27.88%) و خشک گیاه (12.81%)، محتوای نسبی آب برگ (21.04%)، کلروفیل a و b (12.15% و 10.34%) را افزایش داد. بیشترین مقدار فنول کل (34.13 میلی‌گرم بر وزن تر)، عملکرد اسانس (2.12 میلی‌گرم بر وزن تر)، غلظت پرولین (2.52 میلی‌گرم بر وزن تر) و فعالیت آنزیم‌های پراکسیداز (3.15 میلی‌گرم بر وزن تر) و گایاکول پراکسیداز (0.76 میلی‌گرم بر وزن تر) در گیاهان محلول‌پاشی شده با غلظت 150 میلی‌گرم در لیتر اسید جاسمونیک و تخلیه 75% رطوبت ظرفیت زراعی حاصل شد و از سویی بیشترین مقدار کاتالاز (5.12 میلی‌گرم بر وزن تر)، آسکوربات پراکسیداز (0.76 میلی‌گرم بر وزن تر) و سوپراکسید دیسموتاز (5.64 میلی‌گرم بر وزن تر) در گیاهان محلول‌پاشی شده با غلظت ۱۵۰ میلی‌گرم در لیتر اسید جاسمونیک در تنش خشکی (تخلیه 50% و 75% رطوبت ظرفیت زراعی) بدست آمد. بنابراین براساس نتایج بدست آمده به نظر می‌رسد که اسید جاسمونیک با افزایش توان دفاع آنتی‌اکسیدانی و تجمع تنظیم‌کننده‌های اسمزی (نظیر پرولین) سبب کاهش تنش اکسیداتیو در گیاهچه‌های تحت تنش خشکی شده، همچنین با حفظ محتوای نسبی آب برگ و رنگیزه‌های فتوسنتزی موجب بهبود شرایط رشدی بوته‌های مریم‌گلی در شرایط تنش شده است.

کلیدواژه‌ها


- Ahmad, P. and Parasad, M.N.V., 2012. Abiotic Stress Responses in Plants. Springer, New York, 463p.

- Ahmed, S., Nawata, E., Hosokawa, M., Domae, Y. and Sakuratani, T., 2002. Alterations inphotosynthesis and some antioxidant enzymatic activities of mungbean subjected to water logging. Journal Plant Science, 163: 117-123.

- Ardakani, M.R., Abbaszadeh, B., Sharifi Ashourabadi, A., Lebaschi, M.H. and Paknezhad, F., 2007. The effect of water shortages on the quantity and quality of Melissa officinalis. Iranian Journal of Medicinal and Aromatic Plants, 23(2): 251-261.

- Arazmjo, A., Hedari, M., Ghanbari, A., Siasar, B. and Ahmadian, A., 2010. The effect of three types of fertilizers on essential oil, chamomile under drought stress on photosynthetic pigments and osmotic adjustment. Environmental Stresses in Crop Sciences, 3(1): 23-33.

- Asada, K., 2000. The water-water cycle as alternative photon and electron sinks. Philosophical Transactions of the Royal Society Biological Sciences, 355: 1419-1431.

- Babaee, K., Amini Dehaghi, M., Modares Sanavi, S.A.M. and Jabbari, R., 2010. Effect of drought stress on morphological characteristics, amount proline and percent thymol Thymus vulgaris L. Journal of Medicinal and Aromatic Plants of Iran research, 26(2): 239-251.

- Babst, B.A., Ferrieri, R.A., Gray, D.W., Lerdau, M., Schlyer, D.J., Schueller, M., Thrope, M.R. and Orians, C.M. 2005. Jasmonic acid induces rapid changes in carbon transport and partitioning in Populus. Journal New Phytologist, 167: 63-72.

- Bagheri, V., Shamsheri, M.H., Sherani, H. and Rosta, H.R., 2011. Effect of arbuscular mycorrhizal fungi and drought stress on growth, water relations, proline and soluble sugars in plants in two basic varieties of pistachio. Journal of Horticultural Science, 42(4): 365-377.

- Bandurska, H. and Jozwiak, W., 2010. A comparison of the effects of drought on proline accumulation and peroxidases activity in leaves of Festuca rubral L. and Lolium perenne L. Department of Plant Physiology, 79(2): 111-116.

- Bari, R. and Jones, J.D.G., 2009. Role of plant hormones in plant defense responses. Plant Molecular Biology, 69: 473-488.

- Bates, L.S., Waldern, R. P. and Teave, I. D., 1973. Rapid determination of free proline for water stress studies. Plant and Soil, 39: 205-207.

- Beltrano, J. and Ronco, M.G., 2008. Improved tolerance of wheat plants (Triticum aestivum L.) to drought stress and rewatering by thearbuscular mycorrhizal fungus Glomus claroideum: Effect on growth and cell membrane estability. Brazilian Journal of Plant Physiology, 20(1): 29-37.

- Brouki Milan, A., Hassani, L., Abdollahi Mandoulakani, B., Darvishzadeh, R., Kheradmand, F. and Hassani, A., 2016. The effect of different concentrations of methyl jasmonate on the activity of antioxidant enzymes and total protein in basil. Agricultural Crop Management (Journal of Agriculture), 18(1): 103-115.

- Comparot, S.M., Graham, C.M. and Reid, D.M., 2002. Methyl jasmonate elicits a differential antioxidant response in light and dark grown canola (Brassica napus) roots and shoots. Journal Plant Growth Regulation, 38: 21-30.

- Czerpak, R., Piotrowska, A. and Szulecka, K., 2006. Jasmonic acid effects changes in the growth and some components content in alga Chlorella vulgaris. Acta Physiologia Plantarum, 28: 195-203.

- El-Sayed, O.M., El-Gammal, O.H.M. and Salama, A.S.M., 2014. Effect of ascorbic, proline and jasmonic acid foliar spraying on fruit set and yield of Manzanillo olive trees under salt stress. Scientia Horticulturae, 176: 32-37

- Emongor, V., 2007. Gibberellin influence on vegetative growth nodulation and yield of Cowpea (Vigna sp.). Journal Agrobiology, 60(4): 509-517.

- Farmer, E.E., Johnson, R.R. and Ryan, C.A., 1992. Regulation of expression of proteinase inhibitor genes by methyl jasmonate and jasmonic acid. Plant Physiology, 98(3): 995-1002.

- Fedina, I.S. and Benderliev, K.M., 2000. Response of Scenedesmus incrassatulus to salt stress as affected by methyl jasmonate. Biologia Plantarum, 43: 625-627.

- Ghasemzadeh, A., Jaafar, H.Z. and Rahmat, A., 2010. Antioxidant activities, total henolics andflavonoids content in two varieties of Malaysia young ginger (Zingiber officinale Roscoe). Molecules Journal, 15(6): 4324-4333.

- Hemeda, H.M. and Kelin, B.P., 1990. Effects of naturally occurring antioxidants on peroxidase activity of vegetable extracts. Journal of Food Science, 55: 184-185.

- Heredia, J.B. and Zevallos, L.C., 2009. The effects of exogenous ethylene and methyl jasmonate on the accumulation of phenolic antioxidants in selected whole and wounded fresh produce. Food Chemistry, 115: 1500-1508.

- Jiang, Y. and Huang, B., 2000. Effects of drought or heat stress alone and in combination on Kentucky bluegrass. Crop Science, 40(5): 1358-1362.

- Jung, S., 2004. Effect of chlorophyll reduction in Arabidopsis thaliana by methyl jasmonate or norflurazon on antioxidant systems. Journal of Plant physiology and Biochemistry, 42: 231-255.

- Khan, H.U.‚ Link, W., Hocking, T. and Stoddard, F., 2007. Evaluation of physiological biomembranes. Methods in Enzymology, 148: 350-382.

- Kumari, G.J., Reddy, A.M., Naik, S.T., Kumar, S.G., Prasanthi, J., Sriranganayakulu, G., Reddy, P.C. and Sudhakar, C., 2006. Jasmonic acid induced changes in protein pattern, anti oxidative enzyme activities and peroxidase isozymes in peanut seedlings. Biologia Plantarum, 50: 219-226.

- Lebaschy, M.H. and Sharifi ashoor abadi, A., 2004. Indicators Growth some medicinal plants in various conditions of water stress. Iranian Journal of Medicinal and Aromatic Plants, 20(3): 249-261.

- Letchamo, W. and Gosselin, A., 2002. Transpiration, essential oil glands, epicuticular wax and morphology of Thymus vulgaris are influenced by light intensity and water supply. Journal of Horticultural Science, 71(1): 123-134.

- Levitt, J., 1980. Response of Plants to Environmental Stresses (Vol. 2): Water, Radiation, Salt and Other Stresses. Academic Press, New York, 650p.

- Li, L., Staden, V. and Jager, A.K.1998. Effect of plant growth regulators on the antioxidant system in seedling of two maize cultivars subjected to water stress. Journal of Plant Growth Regulation, 25: 81-87.

- Lorio, P.L., 1986. Growth-differentiation balance: A basis for understanding southern pine beetle-tree interaction. Forest Ecology Management, 14: 259-273.

- Ma, Q., Niknam, S.R. and Turner, D.W., 2006. Response of osmotic adjustment and seed yield of Brassica napus and Brassica jounce to soilwater deficit at different growth stages. Australian Journal of Agricultural Research, 57(2): 221-226.

- Mandhanis, S., Madan, S. and Sawhney, V., 2006. Antioxidant defence mechanism under salt stress in wheat seedling. Biologia Plantarum, 50(2): 227-231.

- Martín-Closas, L., Toro, F.J., Calvó, G. and Pelacho, A.M., 2002. Effect of Methyl Jasmonate on the first developmental stages of globe artichoke. International Society for Horticultural Science, Acta Horticulturae 660 5th International Congress on Artichoke, Bari, Italy.

- McDonald, S., Prenzler, P.D., Autolovich, M. and Robards, K., 2001. Phenolic content and antioxidant activity of olive extracts. Food Chemistry, 73: 73-84.

- Mishra, S. and Dubey, R.S., 2006. Heavy metal up take and detoxification mechanisms in plants. International Journal of Agricultural Research, 1(2): 122-141.

- Mittler, R., Vanderauwera, S., Gollery, M. and Vanbreusegem, F., 2004. Reactive oxygen gene network of plants. Trends in Plant Science, 9: 490-498.

- Morello, J.R., Romero, M.P., Ramo, T. and Motilva, M.J., 2005. Evaluation of L-phenylalanine ammonialyase activity and phenolic profile in olive drupe (Olea europaea) from fruit setting period to harvesting time. Plant Science, 168: 65-72.

- Munne, S. and Alegre, L., 2003. Role of dew onthe recovery of water stressed Melissa officinallis. Journal of Plant Physiology, 154(5-6):759-766.

- Nadiu, T. and Naraly, A., 2001. Screening of drought tolerance in green gram (Vigna radiate L. Wilczeek) genotypes under reducing soil moisture. Indian Journal of Plant Physiology, 6(2): 197-201.

- Oliviera-Neto, C.F., Silva-Lobato, A.K., Goncalves-Vidigal, M.C., Costa, R.C.L., Santosfilho, B.G., Alves, G.A.R., Silva-Maia, W.J.M., Cruz, F.J.R., Neres, H.K.B. and Santos Lopes, M.J., 2009. Carbon compounds and chlorophyll contents in sorghum submitted to water deficit during three growth stages. Journal of Food, Agriculture & Environment, 7(3-4): 588-593.

- Omidbaigi, R., 2005. Production and Processing of Medicinal Plants (Vol. 3). Astan Quds Razavi Press, Mashhad, 400p.

- Parra-Lobato, M.C., Fernandez-Garcia, N., Olmos, E., Alvares-Tinaut, M. and Gomez Jimenez, C., 2009. Methyl jasmonate-induced antioxidant defence in root apoplast from sunflower seedlings. Environmental and Experimental Botany, 66: 9-17.

- Petropoulos, S., Daferera, A., Polissiou, D. and Passam, H.C., 2008. The effect of water deficit stress on the growth, yield and composition of essential oils of parsley. Sciential Horticulture, 115(4): 393-397.

- Piotrowska, A., Bajguz, A., Zylkiewicz, B. and Czerpak, R., 2009. Jasmonic acid modulator of lead toxicity in aquatic plant Wolffia arrhiza (Lemnaceae). Environmental and Experimental Botany, 66(3): 507-513.

- Popova, L.P., Ananieva, E., Hristova, V., Christov, K., Georgieva, K., Alexieva, V. and Stoinova. Z.H., 2003. Salicylic acid and methyl jasmonate induced protection on photosynthesis to paraquat oxidative stress. Journal of Plant Physiology, 133-152.

- Prochazka, S., Machaackova, I., Kreekule, J. and Sebanek, J., 1998. Plant Physiology. Academia Praha, 484p.

- Reda, F., Baroty, G.S.A., Talaat, I.M., Abdel Rahim, I.A. and Ayad, H.S., 2007. Effect of some growth regulators and vitamins on essential oil, phenolic content and activity of oxidoreductase enzyme of Thymus vulgaris. Journal Agriculture Science, 30: 630-638.

- Reddy, A.R., Chaitanya, K.V., Jutur, P.P. and Sumithra, K., 2004a. Differential antioxidative response to weather stress among five mulberry cultivars. Environement and Experimental Botany, 52: 33-42.

- Reddy, A.R., Chaitanya, K.V. and Vivekanandan, M., 2004b. Drought-induced responses of photosynthesis and antioxidant metabolism in higher plants. Journal of Plant Physiology, 161(11): 1189-1202.

- Reyes, L. and Cisneros-Zevallos, F., 2003. Wounding stress increases the phenolic content and antioxidant capacity of purpleflesh potatoes. Journal of Agriculture and Food Chemistry, 51: 5296-5300.

- Rubio, V., Bustos, R., Luisa, M.L., Irigoyen, X., Rojas-Triana, M. and Paz-Ares, J., 2009. Plant hormones and nutrient signaling. Plant Molecular Biology, 69: 361-373.

- Sairam, R.K. and Saxena, D.C., 2000. Oxidative stress and antioxidants in wheat genotypes: possible mechanism of water stress tolerance. Journal of Agronomy and Crop Science, 184: 55-61.

- Sairam, R.K., Rao, K.V. and Srivastava, G.C., 2002. Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant Science, 163: 1037-1046.

- Sefidkon, F. and Nazar alipoor, A., 2002. Quantitative and qualitative study of aromatic and medicinal essential oils of fetal chamomile (Tripleurospermum disciforme). Iranian Journal of Medicinal and Aromatic Plants, 6: 33-40.

- Salimi, F., Shekari, F. and Hamzeie, G., 2015. Effect of methyl jasmonat salinity stress on some characteristics of Matricaria chamomilla. College of Horticulture, 29(1): 87-94.

- Shan, C. and Liang, Z., 2010. Jasmonic acid regulates ascorbate and glutathione metabolism in Agropyron cristatum leaves under water stress. Plant Science, 178(2): 130-139.

- Sheraphati Chaleshtari, F., Sheraphati chaleshtari, R. and Momeni, M., 2008. The antimicrobial effects of aqueous extract and ethanol plant Scrophularia striata on E. coli in laboratory. University of Medical Sciences Shaher Kord, 10(4): 32-37.

- Sharma, P. and Dubey, R.S., 2005. Drought induces oxidative stress and enhances the activities of antioxidant enzymes in growing rice seedlings. Journal of Plant Growth Regulation, 46: 209-221.

- Silva, M.A., Jifon, J.L., Silva, J.A.G. and Sharma, V., 2007. Use of physiological parameters as fast tools to screen fordrought tolerance in sugarcane. Brazilian Journal of Plant Physiology, 19: 193-201.

- Simon, J.E., Bubenheim, R.D., Joly, R.J. and Chares, D.J., 2001. Water stress induced alteration in essential oil content and composition of sweet basil. Journal of Essential Oil Research, 4: 71-75.

- Sunkar, R., 2010. Plant Stress Tolerance Methods and Protocols, Humana Press, 382p.

- Szepesi, A., Csiszar, J., Bajkan, S., Gemes, K., Horvath, F., Erdei, L., Deer, A.K., Simon, M.L. and Tari, I., 2005. Role of salicylic acid and jasmonic acid pre-treatments on the acclimation of tomato plants to salt and osmotic stress. Acta Biologica Szegediensis, 49: 123-125.

- Topp, G.G. and Davies, J.L., 1985. Time domain reflectometry (TDR) and its application to irrigation scheduling. Advances in Irrigation, 3: 107-127.

- Tuomi, J., Niemela, P., Haukioja, E. and Neuvonen, S., 1984. Nutrient stress an explan- at ion for plant anti herbivore responses to defoliation. Oecologia, 61: 208-210.

- Wasternack, C., 2007. Jasmonates: An update on biosynthesis, signal transduction and action in plant stress response, growth and development. Annals of Botany, 74: 1090-1093.

- Weydert, C.J. and Cullen, J., 2010. Measurement of superoxide dismutase, catalase, and glutathione peroxidasein cultured cells and tissue. Natural Protocol, 5(1): 51-66.

- Xiao, X., Xu, X. and Yang, F., 2008. Adaptive responses to progressive drought stress in two Populus cothayanapopulations. Silva Fennica, 42: 705-719.

- Xu, Y.C., Zhang, J. B., Jiang, Q.A., Zhou, L.Y. and Miao, H.B., 2006. Effects of water stress on the growth of Lonicera japonica and quality of honeysuckle. Zhongyaocai of Chinese, 29(5): 420-423.

- Yadegari, L., Hedari, R. and Karaptian, N., 2009. The permeability of biological membranes and antioxidant activity of Glycine max in response to low temperature. Iranian Journal of Biology, 2(2): 229-236.