K. Jaimand; M.B. Rezaee; F. Sefidkon; M. Naderi; H. Keneshloo; M.Y. Achak; M. Farahpour; Sh. Karimi
Abstract
Moringa peregrine (Forssk.) Fiori is one of the species growing in tropical and desert areas and is distributed in the southeastern of the country in Hormozghan and Sistan and Balochestan provinces. Moringa peregrine is a desert shrub that can grow in areas with little rainfall, andit has a great nutritional, ...
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Moringa peregrine (Forssk.) Fiori is one of the species growing in tropical and desert areas and is distributed in the southeastern of the country in Hormozghan and Sistan and Balochestan provinces. Moringa peregrine is a desert shrub that can grow in areas with little rainfall, andit has a great nutritional, pharmaceutical, environmental, industrial and economic values. This research was aimed to determination of fatty acids in Moringa peregrina seed oil from different locations in Sistan and Balochestan province in 2009. Five samples were collected from five natural research stations. The oil content was determined by the Soxhlet method. The oil content obtained from Tong Fonoj, Keneshky, Bent, Begaband, and Dorahy Chanf sations were measured to be 54.6%, 50.4%, 52.4%, 53.2%, and 53.6% respectively. According to the obtained results, fatty acids content varied as follows: oleic acid (71.5-74%), palmitic acid (12.6-14.7%), Iso-oleic acid (3.6-4.5%), palmitoleic acid (3.3-4.7%), stearic acid (1.9-2.4%), Behenic acid (0.9-1.5%), gadoleic acid (0.4-10.9%) and Arachidonic acid (0.4-1.3%). Over 90 percent of the oil consumed in the country is imported from abroad. Our results clearly indicate that due to the amount of fatty acids in Moringa peregrina seeds, this plant can be considered as a new source for the production of vegetable oil and widespread food consumption.
K. Jaimand; M.B. Rezaee; Z. Behrad; M. Mirza; V. Mozaffarian; R. Azady; M. Naderi; M. Golipur; A. Bahmanzadegan; S. Meshkizadeh; Sh. Karimi
Abstract
Hypericum genus is one of the most important medicinal plants in Iran. Currently in Iran, there are 17 herbaceous, perennial and shrub species of Hypericum of which three species are endemic to Iran. This research was aimed to investigate Hypericine content in nine species of Hypericum. For Hypericine ...
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Hypericum genus is one of the most important medicinal plants in Iran. Currently in Iran, there are 17 herbaceous, perennial and shrub species of Hypericum of which three species are endemic to Iran. This research was aimed to investigate Hypericine content in nine species of Hypericum. For Hypericine content 1 gram of plant was extracted in two steps, chloroform extraction then methanol extraction using a Soxhlet device. Hypericin content was measured by HPLC, using the following condition, mobile phase: (methanol 68%, ethyl acetate 20% and sodium hydrosulphate (0.1 M) 12%) and stationary phase C18, and UV detector: set on 590 nm. Generally, no hypericin was detected in different organs of H. androsaemum L. Hypericine content detected in flowers, leaves and stems were: H. apricum Kar. & Kir. (in flowers 0.061% and leaves 0.005%), H.armenum Jaub. & Spach (flower 0.003%), H.asperulum Jaub. & Spach (in flower 0.025%, leaves 0.004% and stems 0.003%), in H.hirsutum L. (flower 0.007%), in H.linarioides Boss. (flower 0.007%), in H.tetrapterum Fries (flowers 0.008%, leaves 0.014%, and stem 0.001%), and H.vermiculare Boiss. & Hausskn. (flowers 0.005%), in H.perforatum L. (flowers 0.124%, leaf 0.028% , stem 0.003).
N. Ansari; N. Hasanzadeh; M.B. Rezaee
Abstract
One of the modern methods for biological control of button mushroom (Agaricus bisporus) brown blotch disease is using plant's essential oil. Therefore, antimicrobial properties of essential oil and extracts obtained from Eucalyptus camaldulensis Dehnh. leaves were evaluated against the plant pathogenic ...
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One of the modern methods for biological control of button mushroom (Agaricus bisporus) brown blotch disease is using plant's essential oil. Therefore, antimicrobial properties of essential oil and extracts obtained from Eucalyptus camaldulensis Dehnh. leaves were evaluated against the plant pathogenic bacterium Pseudomonas tolaasii both In vitro and In vivo conditions. 0.3 g/ml of each leaf samples was macerated in water and organic solvents (acetone, methanol and ethanol) to obtain the relevant extracts. The extraction of essential oil from leaves was performed Hydro-distillation method using Clevenger apparatus. Bioassays for inhibition activities of EO were carried out in five concentrations (0/1, 0/01, 0/001, 0/0001 and pure mg/ml) on two agar media of NA and KB. According to the isolation and identification of the main components in essential oils by gas chromatography (GC-MS), Cineol (58.1%) and α-phellandrene (6%) were identified as the main components. The most efficient In vitro results obtained by pure essential oil of Eucalyptus with 17 mm inhibition zone on KB and methanol extract with 8mm on NA. These were more pronounced when compared to inhibition effects of antibiotics erythromycin, penicillin and gentamycin and not with tetracycline in both concentrations of 0.1 and 0.01 mg/ml. This was reversed by subsequent increase of the antibiotics tetracycline and gentamicin to the level of 1, 5 and 10 mg/ml. In vivo assays were conducted in order to evaluate the efficacy of essential oil and methanol extract in two dilutions of 0/1 and 0/01 mg/ml. 20µl of each plant extracts was pre-treated on mushroom caps and after 24 h, the bacterial suspension at ca 105 cfu/ml was inoculated the same pre-treated sites. After a two day incubation period at 25°C, the 0/01 concentration of both extracts showed a satisfactory result.
K. Jaimand; M.B. Rezaee; S.R. Tabaei Aghdaei; M. Nadery Hajibagher Kandy; S. Meshkizadeh
Abstract
In this study, tannin was extracted from rose water, wastewater and petal residue of Rosa damascena Mill. Twenty one accessions collected from different provinces of Iran and cultivated in the Research Institute of Forests and Rangelands were sampled for extraction. Then, each sample of essential oil, ...
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In this study, tannin was extracted from rose water, wastewater and petal residue of Rosa damascena Mill. Twenty one accessions collected from different provinces of Iran and cultivated in the Research Institute of Forests and Rangelands were sampled for extraction. Then, each sample of essential oil, rose water, wastewater and petal residue were prepared. The amount of tannin in the samples was measured by colorimetric method and spectrophotometer apparatus (Model 340 Hitachi) at 760 nm. The highest amount of tannin in rose water, wastewater and petal residue was respectively obtained from Khuzistan and Ilam (2163 ppm), Qom (1846 ppm), and Gillan (1432 ppm) accessions for rose water, Zanjan (1919 ppm), Mazandaran (1895 ppm) Qom, Gilan, Lorestan and Isfahan (1871 ppm) accessions for wastewater, and Yazd (3432 ppm), Sistan and Baluchestan (3139 ppm) and Kohgiluyeh-Boyer Ahmad (2993 ppm) accessions for petal residual. According to the results, it is recommended to extract tannin from samples used in rose water production. In other words, this will be economically useful for rose water traditional producers.
B. Abaszadeh; M.B. Rezaiee; F. Paknejad
Abstract
This research was performed in order to investigate flowering shoot yield, morphological characters, percentage and yield of essential oil and relationship between characters of two ecotypes of Mentha longifolia var. amphilema L. at Research Institute of Forest and Rangelands for six years in 1999 to ...
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This research was performed in order to investigate flowering shoot yield, morphological characters, percentage and yield of essential oil and relationship between characters of two ecotypes of Mentha longifolia var. amphilema L. at Research Institute of Forest and Rangelands for six years in 1999 to 2004. This experiment was conducted as a split plot on time by using of randomized complete block design (RCBD) with three replications. Main and sub factors were ecotypes and years, respectively. The results showed significant differences between leaf oil yields, flower oil yields and flower yields in different ecotypes. There were also significant differences between all evaluated characters (plant height, leaf long, leaf width, stem diameter, lateral shoot, yield of flower, percentage and yield of flower essential oils, leaf yield, percentage and yield of leaf essential oils, yield of flowering shoots and their oils) at 0.01% level of probability in different years. There was significant relationship between total essential oil with yield of flowers and leaves and their essential oils at 0.01%level of probability. There was significant relationship between yield of flowering shoot with leaf long and lateral stem at 0.05% level of probability, and between yield of flowering shoot with leaf yield, essential oil percentage and yield of flower, leaf yield, essential oil percentage and yield of leaf at 0.01% level of probability. The results of stepwise showed that the model compounded of 4 characters: leaf oil yield, flower oil percentage, flower yield and stem diameter. The results of path analysis revealed that leaf oil yield (0.907) had a high direct effect on the dependent (total essential oil yield) variable. So results of path analysis revealed that flower oil percentage (0.019) had a high direct effect on the dependent variable after, yield of leaf essential oil.
S.M. Sharafi; I. Rasooli; T. Allahghadri; M.R. Jalali Nadoushan; M.B. Rezaei
Abstract
In the present study the antimicrobial, antioxidative, hematologic and cytotoxic properties of Citrus limon L. essential oil were studied. The bacterial strains sensitive to Citrus limon L. oil were in the following order: E. coli> K. pneumonia> S. aureus> Streptococcus faecalis> Candida ...
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In the present study the antimicrobial, antioxidative, hematologic and cytotoxic properties of Citrus limon L. essential oil were studied. The bacterial strains sensitive to Citrus limon L. oil were in the following order: E. coli> K. pneumonia> S. aureus> Streptococcus faecalis> Candida albicans> P. aeruginosa .The minimum inhibitory and bactericidal concentrations of the fresh oil were determined. The essential oils had good bactericidal and bacteriostatic properties except for P. aeruginosa. Antioxidative property of the oil was carried out by using beta-carotene bleaching test and the results were compared to the standard synthetic antioxidants. Lipid peroxidation inhibitions were comparable or higher than the synthetic antioxidant BHT and BHA. The oil concentration required for 50% free radical scavenging (IC50) was 22.81 μg/ml with total phenol contents of 57.43 μg GAE/mg for C. limon L. oil. Ferric-reducing antioxidant power (FRAP) in the blood sera of the rats gavaged with a daily dose of 100 µl oil increased. Therapeutic effects were noted as a result of feeding the rats with lemon essential oil. The volatile oil of lemon displayed cytotoxic effects on the human tumor cell line (Hela cells) and peripheral blood cells with the IC50 of 0.97 and 0.57μg/ml respectively. The results showed that the lemon oil might be consumed with precautions after dose determination.
F. Fatemi; A. Allameh; H. Khalafi; M.B. Rezaei; M. Seyhoon
Abstract
Acute lung inflammation is one of the chronic consequences of sepsis which leads to septic patients death. In consider to the side effects of using anti-inflammatory drugs, herbal drugs such as caraway seed has a great potential application with the aim of treating or/and decreasing the consequences ...
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Acute lung inflammation is one of the chronic consequences of sepsis which leads to septic patients death. In consider to the side effects of using anti-inflammatory drugs, herbal drugs such as caraway seed has a great potential application with the aim of treating or/and decreasing the consequences of sepsis. In this study, CLP rat model was used to consider the protective role of hydroalcoholic extract and essential oils derived from caraway seeds in preventing tissue lung injury. In this regards, the effects of caraway extracts on lung oxidative stress parameters i.e. myeloperoxidase, lipid peroxidation and glutathione in different groups of rats were considered. Furthermore, in order to consider the effects of γ-irradiation on pharmacological properties of caraway extracts, four groups of rats treated with irradiated caraway seed extracts and the lung tissues were analyzed using oxidative stress parameters. The results of this study showed that caraway essential oils could modulate the oxidative stress parameters in this experimental acute inflammation; whereas, hydroalcoholic extract did not have any effects on these factors. γ-irradiation of caraway seeds at 10 and 25 kGy also had no effect on these properties of caraway extracts, so that essential oils but not hydroalcoholic extract derived from irradiated caraway seeds could amend oxidative parameters. The results of this study indicated that caraway essential oils could affect the consequences of acute lung injury and also preservation of caraway seeds by irradiation doesn't change its effects.
K. Jaimand; M.B. Rezaee; M.H. Asareh; S.R. Tabaei Aghdaei; S. Meshkizadeh
Abstract
Flavonoid components are the largest phenolic groups in nature. Pigments are primarily responsible for the color of rose petals (the colorless flavonoids aid the intensification and stabilization of petals color). The aim of this research was extraction and determination of flavonoid compounds kaempferol ...
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Flavonoid components are the largest phenolic groups in nature. Pigments are primarily responsible for the color of rose petals (the colorless flavonoids aid the intensification and stabilization of petals color). The aim of this research was extraction and determination of flavonoid compounds kaempferol and quercetin in petals of Rosa damascena from western of Iran and cultivated in Research Institute of Forests and Rangelands. Samples were collected on late April 2006. A 5 g fresh flower petals was blended with 30 ml of 9:1 methanol:acetic acid, the extract was analyzed by high performance liquid chromatography (HPLC). Two flavonoids kaempferol and quercetin glycoside compounds were extracted from ten samples. The highest kaempferol content were obtained from Kordestan (595 ppm), Qazvine (505 ppm), West Azarbaijan (484 ppm), and Ilam (483 ppm), and the lowest from Kermanshah (74 ppm), Hamedan (88 ppm) and Lorestan (Poldokhtar) (252 ppm). The highest quercetin glucoside contents were obtained from Ardabil (322 ppm), West Azarbaijan (312 ppm), Ilam (302 ppm), and Chahar Mehal (239 ppm), and lowest were from Lorestan (Poldokhtar) (77 ppm), Hamedan (80 ppm), Kermanshah (117 ppm) and Kohkiluyeh (Gachsaran) (158 ppm). According to these results, the best samples were obtained from west Azarbaijan (484 ppm kaempferol, 312 ppm quercetin), Ilam (483 ppm kaempferol, 302 ppm quercetin) and Ardabil (447 ppm kaempferol, 322 ppm quercetin).
M.B. Rezaee; K. Jaimand; V. Mozaffrian
Volume 24, Issue 3 , November 2008, , Pages 271-277
Abstract
The volatile constituents of Anthemis coelopoda Boiss. were isolated by hydrodistillation and analyzed by GC and GC/MS. In this study samples were collected from Gilan province in Rodbar on late May 2003. According to literature, this species was not the subject of research up to now and therefore its ...
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The volatile constituents of Anthemis coelopoda Boiss. were isolated by hydrodistillation and analyzed by GC and GC/MS. In this study samples were collected from Gilan province in Rodbar on late May 2003. According to literature, this species was not the subject of research up to now and therefore its chemical composition is not well known. The major constituents of A. coelopoda Boiss. flower oil were Cis-chrysanthenyl acetate (27.3%), hexyl butanoate (16%), and myrcene (7%), while the leaf oil contained isobornyl formate (30.6%), Trans-ethyl chrysanthemumate (15%) and p-mentha-1,5-diene-8-ol (13.7.4%).
G.H. Nasery; M. Mazandarani; M.B. Rezaee
Volume 24, Issue 2 , August 2008, , Pages 126-134
Abstract
Tanacetum parthenium L. is one of the most important medicinal herbs that grows wild and wide distribution near the river, road and sunny positions in forest and mountainous rangelands in South East of Golestan Province, especially in 800-1250m above the sea level in medium soil texture, neutral PH and ...
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Tanacetum parthenium L. is one of the most important medicinal herbs that grows wild and wide distribution near the river, road and sunny positions in forest and mountainous rangelands in South East of Golestan Province, especially in 800-1250m above the sea level in medium soil texture, neutral PH and organic carbon percent with medium to high. In addition to its ecological and ethnopharmacological needs essential oil composition of this plant were also studied. Artemisia annua, Artemisia absintium, Mentha longifolia, Mentha aquatica, Thymus carmanicus, Cuminum cyminum,Echium amoenum, andSalix alba for sedative of headache and menstrual pain, cold, fever, inflammation and healing wound. Flowering aerial parts of this plant were collected in July 2006 in 1100m of Ziarat mountainous region, 6km far from Gorgan city in Golestan province. The essential oil of different parts of plant obtained by water distillation and analyzed by GC/MS. Camphor (53.5%), a-pinene (17.7%) and a-bisabolol oxide B (10.5%) were the major components of flower oil. But in leaf oil, camphor (38.9%), a-pinene (8.4%), germacrene D (6.3%) and g-terpinene (8.2%) were the main components. The results confirm use of this plant by the rural healers for curing many types of the sedative pain and other ailments.
M. Dashti; M.A. Azarbijani; M.B. Rezaee
Volume 24, Issue 2 , August 2008, , Pages 177-188
Abstract
The purpose of this study was to determine the effect of inhaling alcoholic essential oil of Mentha piperita L. on aerobic power and the time to reach anaerobic threshold in athletes males. The subjects of this study were 20 athletes male (age: 20±4 years, height 175±5Cm, weight 68±4.2 ...
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The purpose of this study was to determine the effect of inhaling alcoholic essential oil of Mentha piperita L. on aerobic power and the time to reach anaerobic threshold in athletes males. The subjects of this study were 20 athletes male (age: 20±4 years, height 175±5Cm, weight 68±4.2 Kg, VO2 max 43.21±3.34 ml/kg.min). They were divided into two groups randomly, inhaling alcoholic essential n=10 and control group n=10. First, subjects of the experimental group inhaled essential oil Mentha piperita and Ethanol for 30 minutes. Then they were tested by Conconi test and immediately after 15 minutes rest they were tested by Bruce test. The subjects of control group were tested in similar way but they inhaled water and alcohol. For comparing aerobic and anaerobic performance data were analyzed by T test for independent groups, but for examining the effect of inhaling odors we used ANOVA test (p<0.05). The results showed inhaling essential oil of Mentha piperita with different concentrations can significantly increase aerobic power and anaerobic threshold (p< 0.012). These was no similar significant differences in physical performance in control group. The benefit of inhaling odors may due to increasing in lung volume and capacity and stimulation of simpatico system. We suggest athletes can use inhaling peppermint odor for developing physical performance.
I. Rasooli; L. Gachkar; D. Yadegarinia; M.B. Rezaei; M. Taghizadeh; M.H. Fakoor; A.M. Allameh
Abstract
Lipid peroxidation is the most important deteriorative effect of free radicals that leads to destruction of cell membrane. The routine use of antioxidants is becoming more limited due to their instability and their probable carcinogenic effects. The use of natural additives and antioxidants in treatment ...
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Lipid peroxidation is the most important deteriorative effect of free radicals that leads to destruction of cell membrane. The routine use of antioxidants is becoming more limited due to their instability and their probable carcinogenic effects. The use of natural additives and antioxidants in treatment of microbial and non microbial diseases is gaining momentum among people. In the present work, we extract and identify the chemical compounds of the essential oils of Mentha spicata L. and Chenopodium ambrosioides.L. Antioxidative property, free radical scavenging capacity and antimicrobial characteristics of oils were then studied. The microorganisms employed in this study were: E.coli, S.aureus, S.enteritidis, L. monocytogenes. The plants were hydrodistilled and the essential oils were extracted. The chemical constituents of the oils thus obtained were identified by GC/MS. Employing disc diffusion and tube dilution methods antimicrobial effects of the oils on were studied. Zones of microbial growth inhibition and Minimum Inhibitory and Bactericidal concentrations (MIC & MBC) of the microorganisms exposed to various dilutions of the oils were determined. Kinetics of microbial death were determined. Antioxidant properties of the oils were tested and their relation to antimicrobial properties of the oils were studied. Chemical analysis lead to identification of 14 and 13 compounds in the essential oils of Mentha spicata and Chenopodium ambrosioides, respectively. The sensitivity of bacteria to the oils were the order of L. monocytogenes> E. coli> S. aureus> S. enteritidis. The antibacterial properties of the essential oils from Mentha spicata leaves were higher than the oils from C. ambrosioides leaves. The D values for E. coli, S. aureus, S. enteritidis and L. monocytogenes exposed to the MBC levels of the essential oils were: Mentha spicata (6.42, 10, 6.42, 6.42) and Chenopodium ambrosioides (2.85, 4.28, 5, 4.28) minutes respectively. The zones of microbial growth inhibitions were not correlated to microbicidal kinetics of the oils. The oils had antioxidant properties equivalent to or higher than synthetic BHA antioxidant. The correlation between antioxidative properties and antimicrobial activities of the oils were studied.
A. Zarezadeh; M.B. Rezaee; A. Mirhosseini; M. Shamszadeh
Volume 23, Issue 3 , November 2007, , Pages 432-442
Abstract
According to national project of "identification and ecological investigation of Aromatic plants" collection and identification of Lamiaceae family Aromatic plants in different regions of Yazd province were done. Then the ecological parameters of different plantations such as pedology, latitude, altitude, ...
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According to national project of "identification and ecological investigation of Aromatic plants" collection and identification of Lamiaceae family Aromatic plants in different regions of Yazd province were done. Then the ecological parameters of different plantations such as pedology, latitude, altitude, slope direct, slope percentage, annual precipitation and mean temperature, climate, dominant species, companion species and the best habitat with respect to abundance and density were recorded. The results showed, out of 71 aromatic plant species, 34 belonged to 15 genera related to Lamiaceae family. The important genera are Nepeta with 9 species and Salvia with 7 species. Biological forms of species include:Hemichryptophytes 52.9 %, Cryptophytes 11.8 %, Therophytes 20.6 %, Chamaephytes 11.8 % and phanerophytes 2.9 %. Hemichryptophytes have the most evential biological form. Most of these species are in high and mountainous areas.
K. Jaimand; M.B. Rezaee; A. Najafi Ashtiany
Volume 23, Issue 2 , August 2007, , Pages 262-268
Abstract
Crocus sativus L. is one of the most important plants in Khorasan provience and exported to the whole world. It is used in food for color and flavour and also in medicine. In this study effects of storage condition of Crocus sativus L. for determining crocin were under investigation in Khorasan ...
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Crocus sativus L. is one of the most important plants in Khorasan provience and exported to the whole world. It is used in food for color and flavour and also in medicine. In this study effects of storage condition of Crocus sativus L. for determining crocin were under investigation in Khorasan Provience. Samples were collected on November 2003. We kept samples for 20 months in three different conditions as light, darkness and refrigerator on zero degree. On November 2005 samples were analyzed by High Performance Liquid Chromatography (HPLC) which crocin percentage content in light were 0.032%, dark 0.038% and refrigerator 0.028%. According to the results the best condition to kept stigmata is darkness.
P. Owlia; H. Saderi; F. Matloob; M.B. Rezaee
Abstract
Staphylococcus aureus is an important pathogen that produces widespread infections. Increasing antibiotic usage for S. aureus infections, created antibiotic resistance and hence need for production of new antibiotics. Medicinal herb with antimicrobial effect have important role in traditional medicine. ...
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Staphylococcus aureus is an important pathogen that produces widespread infections. Increasing antibiotic usage for S. aureus infections, created antibiotic resistance and hence need for production of new antibiotics. Medicinal herb with antimicrobial effect have important role in traditional medicine. The aim of this study was to survey antimicrobial effect of hydroalchoholic extract of Zataria multiflora on S. aureus. The minimal inhibitory concentration (MIC) and minimal bactericidal concentratioin (MBC) of extract of dried leaf of Z. multiflora were determined by macro-dilution method. The changes of the number of bacteria in the presence of different concentrations (25% and 30%) of extract and time were assessed. The results were compared with changes of the number of bacteria in presence of 4 µg/mloxacillin. The results showed that MIC and MBC was equal 25% for extract of Z. multiflora and reduction of the number of bacteria on the present of 30% of extract after 8h is equal with reduction of the number of bacteria exposed to 4 µg/ml oxacillin after 4h. Z .multiflora is native for Iran. Further studies and research on the antimicrobial effects of this medicinal herb, probably, may lead to production of herbal drugs.
M.H. Assareh; Z. Abravesh; M.B. Rezaee
Abstract
The leaves of Eucalyptus caesia were collected from north khuzistan in March 2003. The essential oil of Eucalyptus caesia (Myrthaceae) was prepared by hydro-distillation and analyzed by GC and GC/MS. The essential oil was produced at yield of 0.97% (based on dry weights). Twenty-one components were identified, ...
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The leaves of Eucalyptus caesia were collected from north khuzistan in March 2003. The essential oil of Eucalyptus caesia (Myrthaceae) was prepared by hydro-distillation and analyzed by GC and GC/MS. The essential oil was produced at yield of 0.97% (based on dry weights). Twenty-one components were identified, among them α-pinene (9.3%), 1,8-cineole (69.4%), trans-pinocarveole (2.4%), caryophyllene (6.1%) and globulol (2.8%) were the major constituents.
K. Jaimand; M.B. Rezaee; Z. Abravesh; M. Golypour; M. Sharifee
Abstract
Oleuropein, a compound that cause the bitter taste of olive, has many pharmacological properties. It is a natural antioxidant. In this study, the leaves of nine cultivars of Olea europaea L. named: Olive Begonia, Olive Gorgan, Olive Dezful, Olive Khshavy, Olive Khoramabady, Olive Dagal, Olive Barbar, ...
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Oleuropein, a compound that cause the bitter taste of olive, has many pharmacological properties. It is a natural antioxidant. In this study, the leaves of nine cultivars of Olea europaea L. named: Olive Begonia, Olive Gorgan, Olive Dezful, Olive Khshavy, Olive Khoramabady, Olive Dagal, Olive Barbar, Olive Zahedy and Olive Red were collected from Fadak station in Dezful city (Khozestan province, western south of Iran) at September 2004. After extraction of leaves by methanol, the oleuropein contents of extracts were determined by HPLC. Maximum amount of oleuropein was found in Olive Khoramabady (0.08 mg/ml, 0.24% w/w) and minimum in Olive Dagal (0.04 mg/ml, 0.13% w/w).
K. Jaimand; M.H. Assareh; M.B. Rezaee; M.M. Brazandeh
Abstract
Two Eucalyptus species , E. stricklandii Maiden. and E. erythrocorys F. Muell were cultivated on 1994 in south-western of Iram (Khozestan), were collected on April 2004. The essential oils isolated by hydrodistillation from the leaves of were analyzed by GC and GC/MS.The main components identified ...
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Two Eucalyptus species , E. stricklandii Maiden. and E. erythrocorys F. Muell were cultivated on 1994 in south-western of Iram (Khozestan), were collected on April 2004. The essential oils isolated by hydrodistillation from the leaves of were analyzed by GC and GC/MS.The main components identified in E. stricklandii oil were 1,8-cineole (72.2%), a-pinene (12.2%) and terpinen-1–ol (2.8%), and in E. erythrocorys were 1,8-cineole (80%), a-pinene (5.7%) and terpinen-1-ol (2.2%). High content of 1,8-cineole in our cultivated plants and uses of this compound in pharmacy industry is a reason of our investigation. This paper now reportes for the first time the results of the analyses of leaf oils of E. stricklandii Maiden.,and E. erythrocorys F. Muell. , growing in Iran.
K. Jaimand; M.B. Rezaee; M.H. Assareh; M.M. Brazandeh
Abstract
In this research, essential oils were extracted by hydro-distillation method but with different apparatus of distillation from Rosa damascena Mill. The oil obtained at different yields for example for Clevenger (0.008%), Miquel (0.014%), A.O.A.C. (0.012%) and two plans designed in Research Institute ...
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In this research, essential oils were extracted by hydro-distillation method but with different apparatus of distillation from Rosa damascena Mill. The oil obtained at different yields for example for Clevenger (0.008%), Miquel (0.014%), A.O.A.C. (0.012%) and two plans designed in Research Institute of Forests and Rangelands by authors which were named plan-1 (0.015%) and plan-2 (0.023%). Samples were analyzed by GC and GC/MS. The main constituents of the oil by Clevenger were geraniol (26.7%), n-nonadecane (21.3%), citronellol (12.5%) and n-henicosane (12.0%); by Miquel were geraniol (32.9%), citronellol (19.0%), n-nonadecane (10.6%); by A.O.A.C. were n-nonadecane (28.5%), geraniol (22.6%), n-henicosane (10.6%) and citronellol (10.2%) in plan -1 were n-nonadecane (21.3%), geraniol( 21.8%), citronellol (12%) and in plan-2 were n-nonadecane (21.8%), geraniol (19.1%) and citronellol (15%), respectively. By comparison of the results from different apparatus of hydro-distillation, it can be concluded that the best plan for obtaining essential oil from Rosa damascena Mill., were plan-2 and Miquel.
A. Akbarinia; M. Khosravifard; M.B. Rezaee; E. Sharifi Ashoorabadi
Abstract
In order to study the autumn and spring cultivation of Fennel (Foeniculum vulgare), Ajowan (Carum copticum), Black cumin (Nigella sativa) and Anis (Pimpenella anisum) under irrigation and non-irrigation conditions an experimental bulk design with 4 replications was conducted in Alamut medicinal ...
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In order to study the autumn and spring cultivation of Fennel (Foeniculum vulgare), Ajowan (Carum copticum), Black cumin (Nigella sativa) and Anis (Pimpenella anisum) under irrigation and non-irrigation conditions an experimental bulk design with 4 replications was conducted in Alamut medicinal plant research station in 2000 and 2001. Each medicinal plant was studied in a separate experiment. Treatments were included: autumn + irrigation, autumn + non–irrigation, spring + irrigation and spring + non-irrigation. The results showed significant different responses mentioned plants planting season Fennel and black cumin had earlier spring growth, more shoot, seed weight, and more seed yield because of better establishment in autumn. While ajowan and anis autumn planting could not be successful because of damping off, so their planting can only be practical in spring. All plants were better in respect of height; seed weight and seed yield in irrigation compared with non-irrigation condition. There was less difference between seed yield of fennel in irrigation and non-irrigation condition in autumn planting. Fennel and black cumin had 1703 and 1298 in irrigation, 948 and 447 kg/ha in non-irrigation condition in autumn planting. Also ajowan and anis had 1147, 1120 in irrigation and 312 and 361 kg/ha in non-irrigation condition in spring planting. Result of Pearson Correlation Coefficient about black cumin showed that there were significant correlations among all of the traits.
Z. Abravesh; A. Majd; M.B. Rezaee; S. Mehrabian
Abstract
Man used to take a lot of medicinal plants, but a little to take care of their duration. To find methods for increasing their products always paid attention to plant. Essential oil of the flowering shoot of Ammi visnaga was extracted by hydro-distillation method and the essential oil inhibitory effects ...
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Man used to take a lot of medicinal plants, but a little to take care of their duration. To find methods for increasing their products always paid attention to plant. Essential oil of the flowering shoot of Ammi visnaga was extracted by hydro-distillation method and the essential oil inhibitory effects have been studied by using well diffusion method on four bacteria consists of: Streptococcus viridans, Lactobacillus casei, Lactobacillus plantarum, Lactobacillus acidophilus (control: cetylpyridinium chloride 0.05%). Effective essential oil against above mentioned bacteria suspension were studied by micro organism 108 colony forming unit/ml (cfu/ml). Bactericide activity of essential oil of Ammi visnaga flowering shoot observed later of 24-48 hours. The zones of growth inhibition were as follow: Streptococcus viridans 35mm, Lactobacillus casei 50mm, Lactobacillus plantarum 35mm, Lactobacillus acidophilus 35mm and control 12mm.
K. Jaimand; M.B. Rezaee; A.N. Ashtiany
Abstract
Phenolic compounds in olive fruits are important factors to consider. These compounds are partly responsible for auto-oxidation stability and organoleptic characteristics. Moreover, they have pharmacological properties and are natural antioxidants and inhibit gram- positive microorganisms involved in ...
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Phenolic compounds in olive fruits are important factors to consider. These compounds are partly responsible for auto-oxidation stability and organoleptic characteristics. Moreover, they have pharmacological properties and are natural antioxidants and inhibit gram- positive microorganisms involved in the fermentation of olive fruits. Oleuropein, the main component that produced bitterns in olive, is a heterosidic ester of elenolic acid and 3,4-dihydroxyphenylethanol. In this study we have collected two samples in summer 2004 from washing exude of fruits Olea europaea L., after procedure for the separation of phenolic compounds extracted. Identification and determination of oleuropein compound was done by HPLC. The results showed the quantity of oleuropin in the salt water sample was 0.0010% mg/ml and in NaOH sample was 0.0016% mg/ml.
S.R Tabaei-Aghdaei; S. Farhangian; A.A. Jafari; M.B. Rezaee
Abstract
To evaluate 17 Damask rose genotypes regarding their variation for morphological traits, an experiment was conducted at the experimental field of Research Institute of Forests and Rangelands. Analysis of variance, mean comparison and phenotypic correlation analysis were performed. The variance analysis ...
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To evaluate 17 Damask rose genotypes regarding their variation for morphological traits, an experiment was conducted at the experimental field of Research Institute of Forests and Rangelands. Analysis of variance, mean comparison and phenotypic correlation analysis were performed. The variance analysis showed significant differences among genotypes for plant height and flower number per branch (P<0.01), number of leaves per branch, leaflet length and thorn density (P<0.05). Also, comparison of means classified the genotypes for the above characteristics. Significant correlations were observed between different traits. A significant (P<0.01) phenotypic correlation (r=0.96) indicated a strong positive relationship between flower yield and number of flower per plant. From the results, a wide range of variation was observed among the genotypes. Morphological characteristics could, therefore, be useful criteria for selection for yield. However, more genotypes, as well as further analyses, including flower quality especially essential oil components are necessary to be considered, in an efficient breeding of Rosa damascena.
S.R. Tabaei-Aghdaei; M.B. Rezaee; K. Jaimand
Abstract
Rosa damascenaMill. genotypes werecollected from central parts of Iran, and cultivated at the experimental field of Research Institute of Forests and Rangelands, using a randomized complete blocks design with three replications. Genotypes from six provinces were evaluated for essential oils yield and ...
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Rosa damascenaMill. genotypes werecollected from central parts of Iran, and cultivated at the experimental field of Research Institute of Forests and Rangelands, using a randomized complete blocks design with three replications. Genotypes from six provinces were evaluated for essential oils yield and number of floral parts. Genotypes showed differences in terms of the measured traits. Essential oils concentration and yield showed variation. The most essential oils percentage (0.05%) and yield were observed in Isfahan7 and Yazd1 accessions, respectively, in 2002. Yazd2 and Isfahan6 accessions showed the highest essential oils percentage (0.03%) in 2001, and the most percentage of oils was observed in accession collected from Tehran, in 2003. Also, accession of Yazd1 demonstrated the highest 3-year mean of essential oils percentage and yield. Furthermore, the accessions showed variation for floral parts number. The highest numbers of petals and pistils were observed in accession collected from Tehran Province. Also, Isfahan10 accession showed the highest number of stamens. A considerable variation was, therefore, revealed for floral parts and essential oil content of Rosa damascena genotypes from central parts of Iran. It could then be concluded that the Rosa damascena genotypes differ for essential oils content and this may be applied for classification of Damask rose genotypes. Finally yield of essential oils as the major product of Rosa damascena could be considered as an appropriate selection factor, in order to improve rose varieties.
M.B. Rezaee; M. Naderi Hagy Bagher Candy; S.R. Tabaei Aghdaei
Volume 20, Issue 3 , October 2004, , Pages 291-299
Abstract
Contents of inorganic elements are very important traits in plants. Some of elements have causal effects on plant growth. Also, their effects on plant metabolism and therefore quality and quantity of essential oils. Thus, to obtain relatively good results, different parts of Rosa damascena Mill. ...
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Contents of inorganic elements are very important traits in plants. Some of elements have causal effects on plant growth. Also, their effects on plant metabolism and therefore quality and quantity of essential oils. Thus, to obtain relatively good results, different parts of Rosa damascena Mill. genotypes were analyzed. In this research, plants collected from different provinces, including Tehran, Qazvin, East Azarbaijan and Golestan previnces and cultivated in Institute of Forests and Rangelands, and samples collected in May 2003. For determination and comparative study on inorganic elements like Na, K, Mn, Ca, Mg, Zn, Cu, P and N used different apparatus like Induced Coupled Plasma (ICP), Kjeltce and spectrophotometer. Results from different genotypes on petals and sepals of Rosa damascena Mill. from different states like Tehran, East Azarbaijan and Golestan were Na (127.4 – 160.4ppm), K (24.48 – 35.88 ppm), Mg (9.11 – 10.61 ppm), Ca (60.54 – 65.41 ppm), Mn (0.073 – 0.094 ppm), Zn (0.162 – 0.35 ppm), Cu (0.207 0.30 ppm), P (0.19 – 0.28 mg/kg ) and N (0.95 – 1.77 %) in petals, and Na (110.2– 277.7ppm), K (25.72 – 38.53 ppm), Mg (12.36 – 24.27 ppm), Ca (57.63 – 196.3 ppm), Mn (0.105 – 0.185 ppm), Zn (0.156 – 0.62 ppm), Cu (0.166 0.32 ppm), P (0.23 – 0.39 mg/kg ) and N (2.26 – 2.90 %) in sepals.
