Determination of Hypericine content in nine species of Hypericum

Jaimand, K.; Rezaee, M.B.; Behrad, Z.; Mirza, M.; Mozaffarian, V.; Azady, R.; Naderi, M.; Golipur, M.; Bahmanzadegan, A.; Meshkizadeh, S.; Karimi, Sh.

doi:10.22092/ijmapr.2014.5261

Document Type : Research Paper

Authors

¹ Research Institute of Forests and Rangelands, Tehran

² Research Institute of Forests and Rangelands, Tehran, Iran

³ - Research Institute of Forests and Rangelands, Tehran, Iran

⁴ Fars Research Center for Agriculture and Naturual Resources, Shiraz, Iran

https://doi.org/10.22092/ijmapr.2014.5261

Abstract

Hypericum genus is one of the most important medicinal plants in Iran. Currently in Iran, there are 17 herbaceous, perennial and shrub species of Hypericum of which three species are endemic to Iran. This research was aimed to investigate Hypericine content in nine species of Hypericum. For Hypericine content 1 gram of plant was extracted in two steps, chloroform extraction then methanol extraction using a Soxhlet device. Hypericin content was measured by HPLC, using the following condition, mobile phase: (methanol 68%, ethyl acetate 20% and sodium hydrosulphate (0.1 M) 12%) and stationary phase C₁₈, and UV detector: set on 590 nm. Generally, no hypericin was detected in different organs of H. androsaemum L. Hypericine content detected in flowers, leaves and stems were: H. apricum Kar. & Kir. (in flowers 0.061% and leaves 0.005%), H.armenum Jaub. & Spach (flower 0.003%), H.asperulum Jaub. & Spach (in flower 0.025%, leaves 0.004% and stems 0.003%), in H.hirsutum L. (flower 0.007%), in H.linarioides Boss. (flower 0.007%), in H.tetrapterum Fries (flowers 0.008%, leaves 0.014%, and stem 0.001%), and H.vermiculare Boiss. & Hausskn. (flowers 0.005%), in H.perforatum L. (flowers 0.124%, leaf 0.028% , stem 0.003).

Keywords

References

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Iranian Journal of Medicinal and Aromatic Plants Research

Determination of Hypericine content in nine species of Hypericum

References

References

Volume 30, Issue 1 - Serial Number 63
April 2014
Pages 10-18

Determination of Hypericine content in nine species of Hypericum

References

References

Volume 30, Issue 1 - Serial Number 63April 2014Pages 10-18

Volume 30, Issue 1 - Serial Number 63
April 2014
Pages 10-18