Improvement and breeding
B. Yoosefi; S.R. Tabaie-Aghdaie; A. Amiri
Abstract
Forty eight damask rose (Rosa damascena Mill.) accessions from different parts of Iran (11 native accessions of Kermanshah and 37 accessions from other provinces of the country) were cultivated in a randomized complete block design with three replications in Mehregan research station, Kermanshah province ...
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Forty eight damask rose (Rosa damascena Mill.) accessions from different parts of Iran (11 native accessions of Kermanshah and 37 accessions from other provinces of the country) were cultivated in a randomized complete block design with three replications in Mehregan research station, Kermanshah province in 2006-2011 and their yield, morphological, and phenological traits were studied. Each experimental plot consisted of three individual plants with a distance of 3 m × 3 m. The drip irrigation was carried out once every 10 days. Also, the chemical fertilizers and pesticides were not used in the field. The desired traits were recorded and analyzed after the seedlings establishment in the third to fifth years. The results of combined analysis of variance showed that all the studied traits were significantly different (P<0.01) between the experimental years and between the different accessions. The variance of accession × year interaction in all the traits was significant (P<0.01). According to the results, the accessions Kermanshah codes 10, 11, 2, and 3, Kurdistan 1, and Isfahan codes 7 and 4 could be introduced for the cultivation in the climatic conditions of Kermanshah province as the compatible accessions with suitable flower and essential oil yield.
Biotechnology
B. Yousefi; S.R. Tabaei-Aghdaei
Abstract
To identify the appropriate detection methods of stable and adaptable genotypes for essential oil yield, an experiment was conducted on 35 Iranian Rosa damascena Mill. genotypes in a randomized complete block design with three replications in seven locations (Kurdistan, Markazi, Isfahan, Khuzestan, Fars, ...
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To identify the appropriate detection methods of stable and adaptable genotypes for essential oil yield, an experiment was conducted on 35 Iranian Rosa damascena Mill. genotypes in a randomized complete block design with three replications in seven locations (Kurdistan, Markazi, Isfahan, Khuzestan, Fars, Kerman, and Khorasan Razavi provinces) during two years (2007-2008). The total mean of essential oil yield was obtained 0.029%. There were significant differences (P≤0.01) between genotypes, locations, environments or location-years, and also the interactions of genotype × location and genotype × environment in terms of essential oil yield. The positive correlation between environmental variance, coefficient of variation, and regression coefficient of yield over environments with essential oil yield showed that only the low essential oil yield genotypes had the similar phenotype in different environments (static stability). There was no stable genotype with the static regression concept and the essential oil production of stable genotypes by environmental variance was very small, but some adaptable genotypes by the environmental coefficient of variation (e.g. KM1) had moderate essential oil yield and yield stability at the same time. The stable genotypes according to the dynamic stability concept had moderate essential oil yield. The superiority index introduced the genotypes with the highest essential oil as the adaptable ones. The stable genotypes with the lowest variance of the years within locations produced the least essential oil. The results showed that a R. damascena genotype can demonstrate the static and dynamic stabilities with the high essential oil yield at the same time. The coefficient of variation, dynamic aspect of regression statistics, and superiority index could be suggested as the desirable statistics to evaluate different aspects (static and dynamic) of essential oil stability in R. damascena. In general, the genotypes IS3, KZ1, and CM1 with the moderate essential oil yield and its general stability and adaptability could be recommended for Iran.
S.R. Tabaei-Aghdaei; M.B. Rezaee; K. Jaimand
Volume 20, Issue 4 , January 2018, , Pages 533-545
Abstract
Rosa damascene Mill. wascollected from west parts of Iran, and cultivated at the experimental field of Research Institute of Forests and Rangelands, using a three replicated randomized complete blocks design. Seven genotypes, were compared for essential oils yield in 2003 and for petal, stamen, ...
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Rosa damascene Mill. wascollected from west parts of Iran, and cultivated at the experimental field of Research Institute of Forests and Rangelands, using a three replicated randomized complete blocks design. Seven genotypes, were compared for essential oils yield in 2003 and for petal, stamen, and pistil number, in 2003-2004. Genotypes showed significant differences in terms of the measured traits. Comparison of means classified the genotypes in 7, 7 and 7 (in 2003) and 6, 7 and 6 (in 2004) groups, for petal, stamen and pistil number, respectively. Also, essential oils concentration and yield showed genotypic variation. A considerable variation was, therefore, revealed from different analyses in floral parts and essential oils concentration of Rosa damascena Mill. from west parts of Iran. It could then be concluded from the results of different analyses that although the genotypes could be grouped based on geographical origins, concentration and yield of essential oils as the most important product of Rosa damascena Mill., and also some of the related traits, such as floral parts could be used as appropriate selection factors for classification of damask rose genotypes, in order to use in breeding program.
B. Yoosefi; H.R. Ghasempoor; B. Yousefi; S.R. Tabaeii Aghdaie; K. Jaimand
Abstract
Damask Rose (Rosa damascena Mill.) is an important medicinal and industrial species. To achieve more and better-quality essential oil identifying the superior accessions of Damask Rose and their genetic relationships is very important. For a detailed review of the relationship among the genotypes of ...
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Damask Rose (Rosa damascena Mill.) is an important medicinal and industrial species. To achieve more and better-quality essential oil identifying the superior accessions of Damask Rose and their genetic relationships is very important. For a detailed review of the relationship among the genotypes of this species and variations in the essential oil composition, the use of multivariate statistical analysis could be useful. In this study, 25 different accessions of Damask Rose were collected from Kermanshah province and other regions of Iran and were planted in a randomized complete blocks design. The essential oil was extracted from the petals with hydrodistillation method. Essential oil composition was identified by using the gas chromatography and gas chromatography coupled with mass spectrometry and the amount (percentage) of each composition was measured. Essential oil compositions were evaluated using cluster analysis. According to the results of cluster analysis, the accessions were grouped in four groups. Discriminant analysis confirmed the results of cluster analysis. Component analysis showed that the accessions were in four groups and there were four different trends among the accessions studied for the chemical compositions of essential oils. According to the correlation analysis, the two important factors, geraniol and citronellol, which increased the quality of essential oil compounds in damask rose, showed a significant positive relationship with each other and with citronellyl acetate and geranial.
M. Mirzaei; N. Ahmadi; F. Sefidkon; A. Shojaeiyan; A. Mazaheri
Abstract
Damask rose (Rosa damascena Mill.) is one of economically important species of the Rosaceaefamily for production of rose oil and rose water. High respiration rate of harvested flowers resulting from increasing temperature inside the stacks of flowers causes a reduction in the quantity and quality of ...
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Damask rose (Rosa damascena Mill.) is one of economically important species of the Rosaceaefamily for production of rose oil and rose water. High respiration rate of harvested flowers resulting from increasing temperature inside the stacks of flowers causes a reduction in the quantity and quality of essential oil. In order to study the effects of different storage conditions on essential oil yield and composition, we conducted a factorial analysis based on completely randomized design with three replications and factors of storage durations, temperatures, and incubation conditions. In addition, the effects of different duration of distillation process were investigated using a completely randomized design experiment in three replications by considering quality and quantity of extracted essential oils. Identification of chemical compositions of essential oils was performed by GC and GC/MS. The results indicated that the storage of Damask rose petals in water at 4°C, especially for 48 hours, resulted in a good amount of essential oil content as well as compositions compared to the other storage methods and even fresh petals, distillated just after harvesting. Moreover, the essential oil content increased by increasing the duration of distillation, so that a distillation time of 3 hours and 3.5 hours was identified as the best time for the oil extraction process in terms of yield and essential oil compounds.
S.R. Karimi Akandi; A. Banakar
Abstract
Damask rose with scientific name of Rosa damascena Mill. contains essential oils with large medicinal properties. Qualitative and quantitative extraction of essential oils as well as its economic justification depends on appropriate methods of drying. Appropriate method of drying reduces loss and damage ...
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Damask rose with scientific name of Rosa damascena Mill. contains essential oils with large medicinal properties. Qualitative and quantitative extraction of essential oils as well as its economic justification depends on appropriate methods of drying. Appropriate method of drying reduces loss and damage during storage and helps maintain product quality. The purpose of this study was to predict Rose moisture content during the drying process with hot air flowing as a function of temperature at four levels (40, 50, 60 and 70°C) and air velocity at three levels (0.5, 1 and 1.5 m/s), using artificial neural networks. The average initial and final moisture contents were calculated to be 78% and 9%, respectively. The drying process was modeled by mathematical models using matlab and then the moisture content graphs were achieved by excel. Then, the drying process was modeled using neural networks with three inputs including temperature, air velocity and time. Results showed that neural network was more accurate than mathematic models in modeling and predicting the drying process of damask rose and could be used in on-line controlling.
K. Mahdavi Mashaki; A. Moieni; M. Jalali Javaran
Abstract
Rose is known as the queen of flowers. Rosa damask (Rosa damascena Mill.) is utilized as a main source of rose oil and rose water in Iran. Biotechnology methods are considered as a fast and efficient way to produce haploid plants and pure lines. Among the different methods, androgenesis is considered ...
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Rose is known as the queen of flowers. Rosa damask (Rosa damascena Mill.) is utilized as a main source of rose oil and rose water in Iran. Biotechnology methods are considered as a fast and efficient way to produce haploid plants and pure lines. Among the different methods, androgenesis is considered as the most effective procedure, due to its high quantity of microspores in the anthers. In the present study, the effect of medium composition was evaluated on anther culture in two ecotypes of Damask rose, and miniature rose. The results showed that the interaction between H1 medium and mid-uninucleate stage produced the highest callogenesis in Damask rose, Kashan ecotype. Furthermore, the results showed that removing ammonium nitrate and doubling potassium nitrate in the medium produced higher callogenesis. In miniature rose, the medium containing calcium chloride and calcium nitrate produced higher callogenesis. Also, the amino acids improved callogenesis in anther culture of Damask rose, and miniature rose. Moreover, glycine, glutamine and casein hydrolysate were more effective than other studied amino acids. Sucrose was a better sugar compared to sorbitol for callogenesis in the studied genotypes. The chromosome counting and flowcytometery results illustrated that the produced calli were tetraploid (28 chromosomes).
A. Rahamani; M. Mirza; S.R. Tabaei-Aghdai
Abstract
In this project, the effect of chemical fertilizers, organic manure and their combination on the quality of essential oils of Damask Rosa was investigated in Karadj Alborz Research Station. The study was conducted using randomized complete blocks design with 20 independent treatments and three replications ...
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In this project, the effect of chemical fertilizers, organic manure and their combination on the quality of essential oils of Damask Rosa was investigated in Karadj Alborz Research Station. The study was conducted using randomized complete blocks design with 20 independent treatments and three replications in a loam-clay soil with pH =7.9. In this experiment, nitrogen, phosphorus and potash and a combination of trace elements as fertilizer and cow manure were used. Amount of essential oil and quality of essential oil at different treatments were measured in 3 years. Essential oils of petals were extracted by hydrodistillation method in three consecutive years and oil composition was identified in the first and second years of flowering stage using GC and GC/MS. The results of analysis of variance and mean performance showed that the difference among treatments was significant at 1% level of probability. Treatment N80P80K40 and 30 tons of manure per hectare had the highest amount of essential oil. The average amount of essential oil in three consecutive years in this with 0.35 percent was the highest and in control with 0.23 percent was the lowest value. The highest average oil yield in the three years was recorded for treatment N40P40K40, (1395.58 g/ha), and minimum was obtained for the treatment consisting only 40 tons of manure, (636.23 g/ha). After that, the minimum oil yield was recorded for control treatment (868.98 g/ha) in these three years. The composition of essential oils in the first year of flowering showed that Linalool, Geraniol and Citronelllol increased 88, 39 and 36 percent, respectively in treatment N40P40K40, compared with the control. In the second year of flowering, the amount of Geraniol in this treatment increased by 45 percent and that of hexadekanol and heptadekan decreased 45 percent and 30 percent compared with the control.
Z. Nemati Lafmajani; S.R. Tabaei-Aghdaei; M.H. Lebaschi; A.A. Jafari; A. Najafi Ashtiani; M. Daneshkhah
Abstract
In this research, effects of different traits on flower yield of 12 Rosa damascena Mill. accessions from different regions of Iran were analyzed under irrigation (control) and non-irrigation with and without mulch conditions. Randomized complete block designs were applied with three replications at Research ...
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In this research, effects of different traits on flower yield of 12 Rosa damascena Mill. accessions from different regions of Iran were analyzed under irrigation (control) and non-irrigation with and without mulch conditions. Randomized complete block designs were applied with three replications at Research Station of Homand Absard, Damavand, Iran. Flower weight, flower number, flower dry matter percentage, oil percentage, oil yield (per ha), petal weight, plant height and canopy cover were recorded. According to the correlation analysis, flower yield positively correlated with petal weight under non-irrigation with mulch, irrigated (control) and non-irrigation without mulch conditions. The statistical multivariate methods were also applied included multivariate linear regression and path analyses. Using three separate stepwise-regression analysis made it possible to determine the traits with most important impacts on the flower yield, under irrigation (control) and non-irrigation with and without mulch conditions. Flower number, petal weight, oil yield, and oil percentage under non-irrigation without mulch totally explained 91.7% of the variation among genotypes in the regression model. Petal weight, plant cover, and diameter of the species under irrigation (control) totally explained 98.6% of the variation among genotypes in the regression model. Oil percentage, oil yield, petal weight, plant height and flower number per ha under non-irrigation with mulch totally explained 90.6% of the variation among genotypes in the regression model. The results indicated that petal weight has the most direct impact on flower yield based on the path analysis and oil yield showed a negative direct effect on the flower yield under non-irrigation (without mulch) condition. Oil percentage, oil yield, petal weight, plant height and flower number per plant under non-irrigation with mulch, irrigated (control) and non-irrigation without mulch totally explained 95/5% of the variation among genotypes in the regression model. Petal weight had the highest direct impact on flower yield based on the path analysis under irrigation and non-irrigation conditions. Therefore, it is possible to consider petal weight as a magnificent character to improve flower yield of Rosa damascene.
K. Jaimand; M.B. Rezaee; S.R. Tabaei Aghdaei; M. Nadery Hajibagher Kandy; S. Meshkizadeh
Abstract
In this study, tannin was extracted from rose water, wastewater and petal residue of Rosa damascena Mill. Twenty one accessions collected from different provinces of Iran and cultivated in the Research Institute of Forests and Rangelands were sampled for extraction. Then, each sample of essential oil, ...
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In this study, tannin was extracted from rose water, wastewater and petal residue of Rosa damascena Mill. Twenty one accessions collected from different provinces of Iran and cultivated in the Research Institute of Forests and Rangelands were sampled for extraction. Then, each sample of essential oil, rose water, wastewater and petal residue were prepared. The amount of tannin in the samples was measured by colorimetric method and spectrophotometer apparatus (Model 340 Hitachi) at 760 nm. The highest amount of tannin in rose water, wastewater and petal residue was respectively obtained from Khuzistan and Ilam (2163 ppm), Qom (1846 ppm), and Gillan (1432 ppm) accessions for rose water, Zanjan (1919 ppm), Mazandaran (1895 ppm) Qom, Gilan, Lorestan and Isfahan (1871 ppm) accessions for wastewater, and Yazd (3432 ppm), Sistan and Baluchestan (3139 ppm) and Kohgiluyeh-Boyer Ahmad (2993 ppm) accessions for petal residual. According to the results, it is recommended to extract tannin from samples used in rose water production. In other words, this will be economically useful for rose water traditional producers.
M.H. Assareh; Z. Abravesh; S.R. Tabaei Aghdaei
Abstract
Because of the economic and medicinal importance of Rosa damascena Mill. the propagation of this plant was considered. In order to protect the selected genotypes, this experiment was performed by simple layering method during autumn and winter seasons 2007 on new growth twigsof Rosa damascena mature ...
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Because of the economic and medicinal importance of Rosa damascena Mill. the propagation of this plant was considered. In order to protect the selected genotypes, this experiment was performed by simple layering method during autumn and winter seasons 2007 on new growth twigsof Rosa damascena mature shrubs. The branches were laid in sand bed, soaked for 24 h by different concentrations of IBA and NAA hormones. The results showed that the rate of rooting was high in sand bedding material in autumn and winter. The highest rooting percentage was %100 with 50 mgl-1 concentration of IBA in autumn and winter seasons and the lowest of rooting percentage was %53.33 with 50 mgl-1 concentration of NAA in autumn season. The rooting branches were cut from the parent plants after 3 months and planted in pots containing soil: sand: peatmoss complex (1:1:1) in greenhouse conditions, survival percentage was 90%.
K. Jaimand; M.B. Rezaee; M.H. Asareh; S.R. Tabaei Aghdaei; S. Meshkizadeh
Abstract
Flavonoid components are the largest phenolic groups in nature. Pigments are primarily responsible for the color of rose petals (the colorless flavonoids aid the intensification and stabilization of petals color). The aim of this research was extraction and determination of flavonoid compounds kaempferol ...
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Flavonoid components are the largest phenolic groups in nature. Pigments are primarily responsible for the color of rose petals (the colorless flavonoids aid the intensification and stabilization of petals color). The aim of this research was extraction and determination of flavonoid compounds kaempferol and quercetin in petals of Rosa damascena from western of Iran and cultivated in Research Institute of Forests and Rangelands. Samples were collected on late April 2006. A 5 g fresh flower petals was blended with 30 ml of 9:1 methanol:acetic acid, the extract was analyzed by high performance liquid chromatography (HPLC). Two flavonoids kaempferol and quercetin glycoside compounds were extracted from ten samples. The highest kaempferol content were obtained from Kordestan (595 ppm), Qazvine (505 ppm), West Azarbaijan (484 ppm), and Ilam (483 ppm), and the lowest from Kermanshah (74 ppm), Hamedan (88 ppm) and Lorestan (Poldokhtar) (252 ppm). The highest quercetin glucoside contents were obtained from Ardabil (322 ppm), West Azarbaijan (312 ppm), Ilam (302 ppm), and Chahar Mehal (239 ppm), and lowest were from Lorestan (Poldokhtar) (77 ppm), Hamedan (80 ppm), Kermanshah (117 ppm) and Kohkiluyeh (Gachsaran) (158 ppm). According to these results, the best samples were obtained from west Azarbaijan (484 ppm kaempferol, 312 ppm quercetin), Ilam (483 ppm kaempferol, 302 ppm quercetin) and Ardabil (447 ppm kaempferol, 322 ppm quercetin).
K. Ahmadi; F. Sefidkon; M.H. Assareh
Volume 24, Issue 2 , August 2008, , Pages 162-176
Abstract
Rosa damascena Mill. commonly known as rose plant belonging to the family Rosaceae, is an important cultivated aromatic plant. This plant has been cultivated in Iran from many years ago. Due to literature, distillation of rose and obtaining rose water was done in Iran for the first time. In this investigation, ...
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Rosa damascena Mill. commonly known as rose plant belonging to the family Rosaceae, is an important cultivated aromatic plant. This plant has been cultivated in Iran from many years ago. Due to literature, distillation of rose and obtaining rose water was done in Iran for the first time. In this investigation, the effect of different drying methods (shade, oven 30, 40 and 50°C) on quantity and quality of essential oils of three genotypes of Rosa damascena were studies for the first time. The oils of all samples were obtained by hydro-distillation and analyzed by GC and GC/MS. The results showed no significant difference between the oil yields of fresh and dried petals, but there was significant difference between percentages of main aromatic compounds. The oil of fresh petals had the highest percentage of geraniol and citronellol and the lowest percentage of stearoptens (heavy hydrocarbons). Therefore the best time for extraction of essential oil from Rosa damascena, is right after collection. Comparison of oil content and composition of dried samples showed drying in shade was better and produce higher percentage of oil and aromatic compounds.
M.R. Kodori; S.R. Tabaei-Aghdaei
Volume 23, Issue 1 , May 2007, , Pages 100-110
Abstract
This study was conducted at Research Center of Agriculture and Natural Resources of Kerman province in 2004-2006. Nine Damask rose accessions collected from different parts of Kerman province were planted using a three replicated randomized complete block design (RCB). Flower yield, flower number, single ...
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This study was conducted at Research Center of Agriculture and Natural Resources of Kerman province in 2004-2006. Nine Damask rose accessions collected from different parts of Kerman province were planted using a three replicated randomized complete block design (RCB). Flower yield, flower number, single flower fresh and dry weight, flower dry matter percentage, petal/flower weight, petal, stamen and pistil numbers were evaluated. Results of ANOVA showed significant differences among accessions for all the traits. Also, significant correlation was observed between different traits, and flower yield showed maximum correlation with flower number and single flower weight. Comparison of means (Duncan method) classified the accessions into different groups based on the above characteristics. The accession collected from Rafsanjan showed the highest flower yield.
F. Sefidkon; Z. Akbari; M.H. Assareh; Gh. Bakhshi Khaniki
Volume 22, Issue 4 , February 2007, , Pages 351-365
Abstract
Rosa damascena Mill is one of the important Rose species for production of aromatic compounds. The oil and distilled water of Rose are used vastly in medicinal, hygienic-cosmetic and food industries. In Iran, there are vast gardens of Rosa damascena in Kashan, Kerman, Tabriz, Sahand and Fars province. ...
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Rosa damascena Mill is one of the important Rose species for production of aromatic compounds. The oil and distilled water of Rose are used vastly in medicinal, hygienic-cosmetic and food industries. In Iran, there are vast gardens of Rosa damascena in Kashan, Kerman, Tabriz, Sahand and Fars province. In this research, the effect of different extraction methods on yield and chemical composition of four Rosa damascena samples (two samples from national botanical garden of Iran with source of Kashan and Oskou, one sample from Kashan and one sample from Chaloos road) were examined. The aromatic compounds were obtained by four extraction methods consisted of two distillation methods (hydro-distillation and water & steam distillation), extraction with organic solvents (by use of hexane and petroleum ether, individually). The yields of essential oils (from distillation methods) and concrete and absolute (from solvent extraction) were calculated. The oils and absolutes were analyzed by GC and GC/MS. The result showed (except Oskou sample) there is no significant difference between oil and absolute yield, but for all samples, the yield of concrete was higher than the yield of oil and absolute, significantly. Distillation methods produced higher percentage of citronellol and sometimes geraniol, but the valuable compound, phenyl ethyl alcohol, was not found in the oils or exist in very little amount. In solvent extraction methods, the percentage of phenyl ethyl alcohol was considerably high and citronellol and also geraniol were found in the absolutes, of course in lower amounts. There were also some differences between the minor components.
K. Jaimand; M.B. Rezaee; M.H. Assareh; M.M. Brazandeh
Abstract
In this research, essential oils were extracted by hydro-distillation method but with different apparatus of distillation from Rosa damascena Mill. The oil obtained at different yields for example for Clevenger (0.008%), Miquel (0.014%), A.O.A.C. (0.012%) and two plans designed in Research Institute ...
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In this research, essential oils were extracted by hydro-distillation method but with different apparatus of distillation from Rosa damascena Mill. The oil obtained at different yields for example for Clevenger (0.008%), Miquel (0.014%), A.O.A.C. (0.012%) and two plans designed in Research Institute of Forests and Rangelands by authors which were named plan-1 (0.015%) and plan-2 (0.023%). Samples were analyzed by GC and GC/MS. The main constituents of the oil by Clevenger were geraniol (26.7%), n-nonadecane (21.3%), citronellol (12.5%) and n-henicosane (12.0%); by Miquel were geraniol (32.9%), citronellol (19.0%), n-nonadecane (10.6%); by A.O.A.C. were n-nonadecane (28.5%), geraniol (22.6%), n-henicosane (10.6%) and citronellol (10.2%) in plan -1 were n-nonadecane (21.3%), geraniol( 21.8%), citronellol (12%) and in plan-2 were n-nonadecane (21.8%), geraniol (19.1%) and citronellol (15%), respectively. By comparison of the results from different apparatus of hydro-distillation, it can be concluded that the best plan for obtaining essential oil from Rosa damascena Mill., were plan-2 and Miquel.
S.R Tabaei-Aghdaei; S. Farhangian; A.A. Jafari; M.B. Rezaee
Abstract
To evaluate 17 Damask rose genotypes regarding their variation for morphological traits, an experiment was conducted at the experimental field of Research Institute of Forests and Rangelands. Analysis of variance, mean comparison and phenotypic correlation analysis were performed. The variance analysis ...
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To evaluate 17 Damask rose genotypes regarding their variation for morphological traits, an experiment was conducted at the experimental field of Research Institute of Forests and Rangelands. Analysis of variance, mean comparison and phenotypic correlation analysis were performed. The variance analysis showed significant differences among genotypes for plant height and flower number per branch (P<0.01), number of leaves per branch, leaflet length and thorn density (P<0.05). Also, comparison of means classified the genotypes for the above characteristics. Significant correlations were observed between different traits. A significant (P<0.01) phenotypic correlation (r=0.96) indicated a strong positive relationship between flower yield and number of flower per plant. From the results, a wide range of variation was observed among the genotypes. Morphological characteristics could, therefore, be useful criteria for selection for yield. However, more genotypes, as well as further analyses, including flower quality especially essential oil components are necessary to be considered, in an efficient breeding of Rosa damascena.
S.R. Tabaei-Aghdaei; M.B. Rezaee; K. Jaimand
Abstract
Rosa damascenaMill. genotypes werecollected from central parts of Iran, and cultivated at the experimental field of Research Institute of Forests and Rangelands, using a randomized complete blocks design with three replications. Genotypes from six provinces were evaluated for essential oils yield and ...
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Rosa damascenaMill. genotypes werecollected from central parts of Iran, and cultivated at the experimental field of Research Institute of Forests and Rangelands, using a randomized complete blocks design with three replications. Genotypes from six provinces were evaluated for essential oils yield and number of floral parts. Genotypes showed differences in terms of the measured traits. Essential oils concentration and yield showed variation. The most essential oils percentage (0.05%) and yield were observed in Isfahan7 and Yazd1 accessions, respectively, in 2002. Yazd2 and Isfahan6 accessions showed the highest essential oils percentage (0.03%) in 2001, and the most percentage of oils was observed in accession collected from Tehran, in 2003. Also, accession of Yazd1 demonstrated the highest 3-year mean of essential oils percentage and yield. Furthermore, the accessions showed variation for floral parts number. The highest numbers of petals and pistils were observed in accession collected from Tehran Province. Also, Isfahan10 accession showed the highest number of stamens. A considerable variation was, therefore, revealed for floral parts and essential oil content of Rosa damascena genotypes from central parts of Iran. It could then be concluded that the Rosa damascena genotypes differ for essential oils content and this may be applied for classification of Damask rose genotypes. Finally yield of essential oils as the major product of Rosa damascena could be considered as an appropriate selection factor, in order to improve rose varieties.
M.B. Rezaee; M. Naderi Hagy Bagher Candy; S.R. Tabaei Aghdaei
Volume 20, Issue 3 , October 2004, , Pages 291-299
Abstract
Contents of inorganic elements are very important traits in plants. Some of elements have causal effects on plant growth. Also, their effects on plant metabolism and therefore quality and quantity of essential oils. Thus, to obtain relatively good results, different parts of Rosa damascena Mill. ...
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Contents of inorganic elements are very important traits in plants. Some of elements have causal effects on plant growth. Also, their effects on plant metabolism and therefore quality and quantity of essential oils. Thus, to obtain relatively good results, different parts of Rosa damascena Mill. genotypes were analyzed. In this research, plants collected from different provinces, including Tehran, Qazvin, East Azarbaijan and Golestan previnces and cultivated in Institute of Forests and Rangelands, and samples collected in May 2003. For determination and comparative study on inorganic elements like Na, K, Mn, Ca, Mg, Zn, Cu, P and N used different apparatus like Induced Coupled Plasma (ICP), Kjeltce and spectrophotometer. Results from different genotypes on petals and sepals of Rosa damascena Mill. from different states like Tehran, East Azarbaijan and Golestan were Na (127.4 – 160.4ppm), K (24.48 – 35.88 ppm), Mg (9.11 – 10.61 ppm), Ca (60.54 – 65.41 ppm), Mn (0.073 – 0.094 ppm), Zn (0.162 – 0.35 ppm), Cu (0.207 0.30 ppm), P (0.19 – 0.28 mg/kg ) and N (0.95 – 1.77 %) in petals, and Na (110.2– 277.7ppm), K (25.72 – 38.53 ppm), Mg (12.36 – 24.27 ppm), Ca (57.63 – 196.3 ppm), Mn (0.105 – 0.185 ppm), Zn (0.156 – 0.62 ppm), Cu (0.166 0.32 ppm), P (0.23 – 0.39 mg/kg ) and N (2.26 – 2.90 %) in sepals.
S.R. Tabaei-Aghdaei; M. Sahebi; A.A. Jafari; M.B. Rezaee
Volume 20, Issue 2 , July 2004, , Pages 199-211
Abstract
In order to study the genetic variation in Rosa damascena Mill., an experiment was conducted at the experimental field of Research Institute of Forests and Rangelands. Eleven genotypes were evaluated, using a randomized complete blocks design with three replications. Flower yield, flower number, ...
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In order to study the genetic variation in Rosa damascena Mill., an experiment was conducted at the experimental field of Research Institute of Forests and Rangelands. Eleven genotypes were evaluated, using a randomized complete blocks design with three replications. Flower yield, flower number, single flower weight, plant height, canopy, branch angel, leaf number, spine density and spine length, were analyzed, using analysis of variance, principal components analysis and cluster analysis. The results showed significant differences among genotypes for all of the traits, indicating a considerable genetic variation in germplasm available in Iran. Using principal components analysis, the first three components determined 68.43% of the total variation. According to the cluster analysis, genotypes could be grouped into 3 clusters. Genotypes in cluster 1 averaged well above the overall mean for productivity and the other characteristics.
S.R. Tabaei-Aghdaei; M.B. Rezaee; M. Jebelly
Volume 20, Issue 1 , April 2004, , Pages 111-122
Abstract
Rosa damascena Mill. genotypes from different areas of Iran, were cultivated at the experimental field of Research Institute of Forests and Rangelands, using a three replicated randomized complete blocks design. 11 genotypes were compared based on flower yield and morphological characteristics, ...
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Rosa damascena Mill. genotypes from different areas of Iran, were cultivated at the experimental field of Research Institute of Forests and Rangelands, using a three replicated randomized complete blocks design. 11 genotypes were compared based on flower yield and morphological characteristics, including plant height, canopy, spine density , spine length, branch angel and leaf number. Evaluation of the obtained data was performed using ANOVA, mean classification and correlation analysis. Genotypes showed significant differences in terms of the measured traits. Comparison of means, classified the genotypes in 3, 4 and 3 groups, based on flower yield, flower number, and average flower weight, respectively. Also, the genotypes were classified in different groups, based on morphological characteristics. The results also indicated that there is a good relationship between some of the measured traits, particularly between yield and number of flowers. It could be concluded from the results of different analyses that although the genotypes could be grouped based on geographical origins, flower yield and number and some of the morphological characteristics could be used as appropriate selection factors for classification of damask rose genotypes, in order to use in breeding programs.
M.B. Rezaee; K. Jaimand; S.R. Tabaei-Aghdaei; M.M. Brazandeh; S. Meshkizadeh
Volume 19, Issue 4 , January 2004, , Pages 339-348
Abstract
Rosa damascena Mill is cultivated in different parts of Iran, and essential oil and rose water in large scales are produced. Essential oil is used in aromatic, food and cosmetics industrials. In this study, esential oils were extracted by hydrodistillation method, from Rosa damascena Mill. Genotypes ...
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Rosa damascena Mill is cultivated in different parts of Iran, and essential oil and rose water in large scales are produced. Essential oil is used in aromatic, food and cosmetics industrials. In this study, esential oils were extracted by hydrodistillation method, from Rosa damascena Mill. Genotypes (6A and 188B) collected from Kashan (Ghamsar) and west Azarbaiejan (Oskoo), respectively and cultivated in Research Institute of Forests and Rangelands. Samples were analyzed by GC and GC/MS. The main constituents in Ghamsar sample were n-nonadecane (25.5%), citronellol (17.7%)and geraniol (13.3 %) and for Oskoo sample were citronellol (27%), geraniol (18%) and n- nonadecane (14.2%). An appropriate was therefore genotype identified with high percent of major essential components, from Oskoo, despite cultivation in a different envirment from its geographical origin.
M.B. Rezaee; K. Jaimand; S.R. Tabaei-Aghdaei; M.M. Brazandeh
Volume 19, Issue 1 , May 2003, , Pages 63-72
Abstract
Rosa damascena Mill is cultivated in different parts of Iran, and essential oil and rose water in large scales are produced. Essential oil is used in aromatic, food and cosmetics industrials. In this study, esential oils were extracted by hydrodistillation method, from Rosa damascena collected from and ...
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Rosa damascena Mill is cultivated in different parts of Iran, and essential oil and rose water in large scales are produced. Essential oil is used in aromatic, food and cosmetics industrials. In this study, esential oils were extracted by hydrodistillation method, from Rosa damascena collected from and cultivated in Research Institute of Forests and Rangelands. Components of this essence were compared to that of industrial samples extracted by the same method, from Rosa damacena cultivated in Kamoo. Samples were analyzed by GC and GC/MS. The main constituents in industrial sample were phytol (39.3%), n-henicosane (16.5%), hexadecane (12.5%), n-heptadecane (5.9%) and citronellol (4.1%)and for laboratory sample were n-nonadecane (21%), citronellol (20.3%), geraniol (19%) and phenylethyl alcohol (18.9%).
