Volume 38, Issue 4 , December 2022
Phytochemistry (extraction, identification and measurement of active components)
P. Arvin; R. Firouzeh
Abstract
To evaluate the biological potential and medicinal properties, the biochemical compounds and quantity and essential oil quality of Chenopodium botrys L. grown in the natural habitat located in Raz and Jargalan city, North Khorasan province was investigated. Leaves or flowering branches sampling was done ...
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To evaluate the biological potential and medicinal properties, the biochemical compounds and quantity and essential oil quality of Chenopodium botrys L. grown in the natural habitat located in Raz and Jargalan city, North Khorasan province was investigated. Leaves or flowering branches sampling was done at full flowering stage. The content of biochemical compounds, including phenolic compounds, flavonoids, and antioxidant capacity, was measured. The essential oil of flowering branches and leaves together was extracted by water distillation method and Clevenger apparatus and analyzed by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). The results showed that the content of phenols, flavonoids, and antioxidant capacity of leaves or flowering branches methanol extracts was obtained 83.2 and 91.4 (mg GA.g-1 DW), 14 and 17 (mg QUE.g-1 DW), and 91 and 77 (μg.ml-1), respectively. Twenty-six compounds were identified in the essential oil. Elemol (17.2%), juniper camphor (7.9%), and bulnesol (6.9%) were the main compounds of essential oil. Also, the essential oil content was obtained 0.36% (w/w). Also, the leaves extract had significantly higher content of anthocyanin (3.1 mg cyanidin-3-glucoside.g-1 DW) compared to the flowering branches one. Overall, based on the results it can be concluded that Ch. botrys is a promising source of antioxidant compounds and is expected to be used in the food, medicine, and health products.
Agriculture and horticulture
S. Heidari; J. Sarhadi; M. Sharif
Abstract
To investigate the effects of organic and chemical fertilizers and their combination on the characteristics of henna (Lawsonia inermis L.) in Southern Kerman province, a factorial farm experiment was conducted in a randomized complete block design with three replications for two years. The factors included ...
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To investigate the effects of organic and chemical fertilizers and their combination on the characteristics of henna (Lawsonia inermis L.) in Southern Kerman province, a factorial farm experiment was conducted in a randomized complete block design with three replications for two years. The factors included rotten cattle manure at two levels (0 (a1) and 15 (a2) ton.ha-1) and NPK fertilizer (urea, triple superphosphate and potassium sulfate) at five levels (0 (b1), 25% (b2), 50% (b3), 75% (b4), and 100% (b5) of the soil test basis). At the end of each year, leaf yield and nitrogen, phosphorus, potassium, and lawson concentrations of the leaves were measured in the different treatments. The results of two-year combined analysis showed that the interaction between organic and chemical fertilizer treatments was significant on yield and leaf lawson concentration. The highest leaf dry weight (483.2 g.m-2) was related to the a2b5 treatment which was not significantly different from a2b4 (473.7 g.m-2). The a2b5 and a2b4 treatments had 41.2% and 38.5% better yield than the control, respectively. Also, the a2b5 treatment showed the highest leaf lawson content (30.1 g.plant-1) without significant difference with a2b4 (25.2 g.plant-1). Considering the situation of the study region in terms of non-use of organic fertilizers and also the economic justification of the produced henna, it could be recommended to use 15 ton.ha-1 cattle manure with a 25% reduction of chemical fertilizer NPK for the henna production.
Phytochemistry (extraction, identification and measurement of active components)
M. Tavakoli; S. Soltani; M. Tarkesh Esfahani; R. Karamian
Abstract
The Salvia species are of great importance in food, pharmaceutical, and cosmetic industries for their secondary metabolites. Although the production of secondary metabolites is encoded by the plant genetic, however, environmental factors have a great influence on their production and composition in plants. ...
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The Salvia species are of great importance in food, pharmaceutical, and cosmetic industries for their secondary metabolites. Although the production of secondary metabolites is encoded by the plant genetic, however, environmental factors have a great influence on their production and composition in plants. In this study, to investigate the effects of some climatic factors, topography, and soil parameters on Salvia multicaulis essential oil composition, the plant samples were collected from nine different habitats in Hamadan province during the flowering season. The soil samples were also taken for each plant in the sampling sites. The essential oil was extracted by hydrodistillation (Clevenger apparatus) and its composition was identified by GC and GC/MS. The soil factors were measured according to the relevant protocols and the climatic records were collected from the meteorological stations of Hamadan province. The RDA analysis was used to investigate the effects of different environmental factors on the essential oil composition. Clustering of the studied habitats was performed based on the major compounds of essential oils using the Wards method. The essential oil composition was different in different habitats. The compounds α-pinene (1.3-32.1%), α-phellandrene (1.1-7%), 1,8-cineole (1-17.2%), bornyl acetate (7-16.2%), and β-caryophyllene (9.1-40.6%) were the major ones of this plant essential oil. The RDA results showed that the essential oil content and oxygenated terpenoids percentage increased with increasing altitude, but the hydrocarbon terpenes percentage had an inverse correlation with altitude. The monoterpenes had a positive correlation with the relative humidity, rainfall, and soil organic carbon, but the sesquiterpenes had an inverse relationship with these factors and showed a positive correlation with phosphorus and to some extent with calcium of the soil. The studied habitats clustered into two distinct groups with predominance of monoterpenes and sesquiterpenes in the essential oil. These findings could be important for commercial exploitation of sage with the aim of extracting specific secondary metabolites in its essential oil.
Biotechnology
A. Shayan; M. Shokrpour; V. Nazeri; M. Babalar; A. Mehrabi
Abstract
Cultivation development of adaptable Thymus daenensis Celak to specific environmental conditions through population improvement requires the creation of a genetic pool rich of superior genotypes and exploitation of this diversity to identify genotypes with high combinability. In this research, to study ...
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Cultivation development of adaptable Thymus daenensis Celak to specific environmental conditions through population improvement requires the creation of a genetic pool rich of superior genotypes and exploitation of this diversity to identify genotypes with high combinability. In this research, to study the genetic diversity and combinability of different T. daenensis ecotypes under salinity, a factorial greenhouse experiment was conducted in a randomized complete block design with three replications. The factors included salinity stress (0 and 90 mM NaCl) and ecotype (12 half-sib families derived from a polycross between 12 T. daenensis ecotypes). Salinity decreased all the measured traits (except for proline and carotenoids content) significantly compared to the control. The ecotypes differed significantly from each other in terms of length and leaf width, length of lateral branches, aerial parts dry weight, essential oil content, and proline content. Essential oil content and leaf width obtained the highest phenotypic and genotypic coefficient of variation. General heritability of the studied traits varied from 4.61% (leaf length) to 81.07% (leaf width). The highest general heritability was assigned to leaf width, essential oil content, and aerial parts dry weight. Regarding the higher genotypic and phenotypic coefficient of variation, heritability, and genetic advance of the traits including essential oil content and aerial parts dry weight, these traits could be considered as the most important criteria for the parents selection in the breeding programs of T. daenensis. The results showed that the ecotypes Malayer2, Zagheh, Arak, and Ilam had high combinability in terms of essential oil content. Considering the sufficient genetic diversity observed between the studied ecotypes along with their adequate combinability, it could be recommended to breed T. daenensis through the strategy of improving the population by producing synthetic cultivars for normal (non-stress) and salinity conditions.
Biotechnology
M. Ahmadi-Roshan; G. Karimzadeh; S. Rashidi monfared
Abstract
Satureja bachtiarica Bunge is one of the endemic Satureja species in Iran, which has strong antioxidant and antimicrobial properties as well as economic and medicinal vlaue due to the presence of compounds like carvacrol and thymol in the essential oil and rosmarinic acid and other phenolic acids in ...
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Satureja bachtiarica Bunge is one of the endemic Satureja species in Iran, which has strong antioxidant and antimicrobial properties as well as economic and medicinal vlaue due to the presence of compounds like carvacrol and thymol in the essential oil and rosmarinic acid and other phenolic acids in the extract. In this study, 10 shooting and 8 rooting treatments were used in MS medium to produce clones of this species using micropropagation method. The shooting treatments were a combination of cytokinins (Kin, 2ip, and TDZ) and auxins (IBA and BA) with different concentrations. The rooting treatments were also a combination of different concentrations of auxins (IBA and NAA). The best shooting treatment [IBA (0.01 mg l-1), TDZ (0.05 mg l-1), and 2ip (0.3 mg l-1)] was recognized by examining traits such as number and length of main and secondary shoots, number of leaves, number of lateral active buds, and leaf colour. Number of main and lateral roots were also criteria for the best rooting treatment [IBA (0.5 mg l-1) and NAA (0.5 mg l-1)] selection. Since S. bachtiarica is an allogamous plant and has a high diversity for different traits like metabolites, practical purpose of the present study was mass production of identical individuals for use in breeding programs. This purpose was done by presenting the micropropagation protocol of this valuable plant.
Agriculture and horticulture
S.S. Zare Pak Ziaberi; M. Majidian
Abstract
To investigate the effects of zinc, boron, and sulfur on the quantitative characteristics, elements uptake, and allicin content in Ziabar local garlic (Allium sativum L.), an experiment was conducted as a completely randomized block design with three replications in 2019-20. The treatments included 1- ...
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To investigate the effects of zinc, boron, and sulfur on the quantitative characteristics, elements uptake, and allicin content in Ziabar local garlic (Allium sativum L.), an experiment was conducted as a completely randomized block design with three replications in 2019-20. The treatments included 1- control (without fertilizer use), 2- Zn, 3- B, 4- S, 5- Zn+S, 6- Zn+B, 7- B+S, and 8- S+B+Zn. Sulfur, zinc, and boron fertilizers were added to the soil as sulfur flower (100 kg ha-1), ZnSO4·7H2O (20 kg ha−1), and H3BO3 (5 kg ha−1) at two vegetative growth and bulb filling stages. The results showed that the S+B+Zn treatment was significantly superior to other treatments in all studied traits. Among the single fertilizer treatments, the highest economic yield and boron uptake rate was observed with the B application. The S application resulted in the highest S uptake. The highest Zn uptake and allicin content was observed in the Zn application treatment. Among the dual treatments, the B+S treatment caused the highest economic yield and S and B uptake rate. The Zn+B treatment showed the highest Zn uptake. The highest allicin content (29%) was observed in the S+B+Zn treatment. The highest garlic economic yield (3000 kg ha-1) and the best quality in terms of allicin content (29%), S uptake (0.73 kg ha-1), Zn uptake (39.6 kg ha-1), and B uptake (12.96 kg ha-1) were obtained in the S+B+Zn treatment. According to the present results, the use of zinc, boron, and sulfur fertilizers along with basal fertilizers could be recommended to increase the quantitative and qualitative yield of garlic.
Agriculture and horticulture
S. Aghighi; Gh. Mohammadi-Nejad; Sh. Khandani
Abstract
Fusarium wilt disease is the most important disease of cumin (Cuminum cyminum L.) and causes great damage to it every year. The present study was conducted to evaluate the response of 13 cumin genotypes to Fusarium wilt disease as a factorial experiment in a completely randomized design (genotype as ...
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Fusarium wilt disease is the most important disease of cumin (Cuminum cyminum L.) and causes great damage to it every year. The present study was conducted to evaluate the response of 13 cumin genotypes to Fusarium wilt disease as a factorial experiment in a completely randomized design (genotype as the first factor and absence and presence of the pathogen as the second one) with three replications in the greenhouse of Research and Technology Institute of Plant Production, Shahid Bahonar University of Kerman during growing season of 2020-2021. The results showed that the interaction of genotype and treatment was statistically significant on most of the traits. The highest traits correlation was observed between plant dry weight and number of umbrellas per plant (0.92) under the absence of pathogen conditions and between plant height and number of seeds per plant (0.76) under the presence of pathogen conditions. Cluster analysis (Ward method) regarding the studied traits divided the genotypes into three groups under the absence of pathogen conditions and two groups under the presence of pathogen. Principal component analysis showed that the first two components could explain 80.13% (absence of pathogen) and 84.55% (presence of pathogen) of the variations. Also, biplot analysis showed high diversity of the genotypes as well as confirming the clustering results. The genotype E7r1b1 had the highest plant mortality ratio (100%) under the presence of pathogen. Under the absence of pathogen, the genotype g8r2b2 had the highest yield (0.20 g) and number (75.66) of seeds per plant. Yield and number of seeds per plant decreased significantly under the presence of pathogen. The highest seed yield (0.14 g) under the presence of pathogen was observed in the genotype 2-14-3. Based on the studied traits, the genotypes g5r2B1 and F12r1b1 had the lowest potential compared to the others under the both conditions. In general, the genotypes reacted differently under the absence and presence of pathogen conditions. The genotypes that revealed tolerance to the disease conditions could be introduced as sources of tolerance in further breeding programs.
Biological effects of essential oils and extracts
B.H. Bahador; J. Bahrampour; A. Moosaei; H. Doomari
Abstract
Henna (Lawsonia inermis L.) leaves have unique medicinal properties like antibiotic ones due to the large amounts of phenolics such as tannins. To investigate the effects of feeding with henna leaves on performance, some blood parameters, and liver enzymes of 20 Kermani male lambs, an experiment was ...
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Henna (Lawsonia inermis L.) leaves have unique medicinal properties like antibiotic ones due to the large amounts of phenolics such as tannins. To investigate the effects of feeding with henna leaves on performance, some blood parameters, and liver enzymes of 20 Kermani male lambs, an experiment was conducted in a completely randomized design with five replications. Experimental treatments included (T1) basic diet (BD) (control), (T2) BD+30 g henna leaves (HL), (T3) BD+60 g HL, and (T4) BD+90 g HL. The results showed that although the treatments did not have significant effects on performance, but the highest and lowest amount of feed consumption and food conversion ratio were respectively observed in control and T4, as well as the highest and lowest amount of daily weight gain belonged to T4 and control, respectively. Glucose and blood urea nitrogen concentrations were not affected by the experimental treatments (P≥0.05). Cholesterol, triglyceride, and total protein concentrations were significantly increased with increasing henna leaf amount in the diet (P≤0.05). The lowest concentrations of liver enzymes aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were obtained in T3 (P≤ 0.05). Overall, it was revealed that using different levels of henna in the diet of Kermani lambs did not affect their growth performance and glucose, but significantly increased cholesterol, triglycerides, and total protein and decreased AST and ALT.
Identification, introduction, ecology and ethnobotany
M. Ghorbani; S. Khorasaninejad; Kh. Hemmati; Kh. Ghorbani
Abstract
This study was aimed at comparing Viola spp. populations collected from north and northwest of Iran to investigate the adaptability and feasibility of their domestication under climatic conditions of Gorgan. The whole plant samples together with their rhizomes were collected from nine habitats in Golestan, ...
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This study was aimed at comparing Viola spp. populations collected from north and northwest of Iran to investigate the adaptability and feasibility of their domestication under climatic conditions of Gorgan. The whole plant samples together with their rhizomes were collected from nine habitats in Golestan, Mazandaran, Gilan, East Azerbaijan, and Kermanshah provinces during the growing season. After botanical approval and receiving herbarium code, the rhizomes were cultivated under the same soil and climate with three replications. Their yield components and physiological, morphological, and phytochemical traits were measured after one crop year at flowering stage. The results showed that these populations were from V. alba Besser, V. odorata L., V. sieheana W.Becker, and V. ignobilis Rups. species. All populations produced flowers and seeds after one crop year. The populations differed significantly (P<0.01). V. ignobilis from Kermanshah had the highest amount of root and aerial parts fresh weight, aerial parts dry weight, and leaf area. The highest amount of root and aerial parts length belonged to V. sieheana from Alangdare. V. alba from Ziarat had the highest amount of total phenols (45.74 mg gallic acid per gram of dry extract) and total flavonoids (20.24 mg quercetin per gram of dry extract). The highest amount of chlorophylls a and b, total chlorophyll, carotenoids, and anthocyanins was observed in V. odorata from Bandargaz. The highest antioxidant activity by DPPH and ABTS methods was found in V. alba from Afratakhte and the highest one by FRAP was observed in V. odorata from Qarnabad. The highest amount of total antioxidant was measured in V. alba and V. oderata populations from Ziarat region. Overall, the results proved the considerable traits diversity in Viola spp. populations. This diversity could be due to the high genetic potential among different populations, differences in environmental conditions, or the interaction of population and environment.
Agriculture and horticulture
A. Javanmard; M. Rahimi; M. Amani Machiani; M. Janmohammadi; R. Habibi Machiani
Abstract
To evaluate the effects of different irrigation levels and foliar application of nutrients, on dry matter yield and essential oil content and composition of peppermint (Mentha piperita L.), a field experiment was conducted as a split plot based on a randomized complete block design, with 12 treatments ...
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To evaluate the effects of different irrigation levels and foliar application of nutrients, on dry matter yield and essential oil content and composition of peppermint (Mentha piperita L.), a field experiment was conducted as a split plot based on a randomized complete block design, with 12 treatments and three replications, at the faculty of Agriculture, University of Maragheh, Iran in 2021. The factors included irrigation as the main factor at three levels (irrigation after 20 (control), 40 (moderate stress), and 60% (severe stress) of the maximum allowable moisture depletion) and fertilizer source as the sub-factor at four levels (non-application of fertilizer (control), and foliar application of Macromix gold, Ecoquel micromix, and Vitalem forte). The results demonstrated that the highest (280.5 g.m-2) and lowest (113.7 g.m-2) total dry weight were obtained under normal irrigation conditions (control) with the application of Macromix gold and severe stress without fertilizer application, respectively. Total dry weight decreased by 22.7 and 40.3% under moderate and severe drought stress conditions, respectively compared to normal irrigation. Macromix gold, Vitalem forte, and Ecoquel micromix application enhanced the amount of total dry weight by 37.7, 23.8, and 17.1% compared to no fertilizer application, respectively. The highest content (2.1%) and essential oil yield (4.5 g.m-2) and also the highest amount of menthol (40.9%), menthone (28.1%), and cineole (6.2%) in the essential oil were observerd under moderate water stress conditions fertilized with Macromix gold. Based on the obtained results, the foliar application of Macromix gold could be recommended as an effective solution to enhance the quantity and quality of the peppermint essential oil under drought stress conditions.
Agriculture and horticulture
S. Azizi Balabiglou; A. Rahimi; S. Heydarzadeh; R. Holgoumi; I. Ataei
Abstract
To investigate the response of some quantitative and qualitative characteristics of peppermint (Mentha piperita L.) to organic fertilizers under low water stress, a factorial experiment was conducted in a randomized complete block design with three replications in the research farm of the Faculty of ...
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To investigate the response of some quantitative and qualitative characteristics of peppermint (Mentha piperita L.) to organic fertilizers under low water stress, a factorial experiment was conducted in a randomized complete block design with three replications in the research farm of the Faculty of Agriculture of Urmia University in 2017-2018 crop year. Experimental treatments included organic fertilizer at five levels (control, cattle manure (20 ton ha-1), poultry manure (7 ton ha-1), vermicompost (10 ton ha-1), and compost (15 ton ha-1)) and soil moisture at three levels (50 (severe stress), 70 (moderate stress), and 90% (control: without stress) of field capacity). Low water stress decreased the content of leaf phosphorus or potassium, leaf phenols or flavonoids, and leaf relative water content and increased the leaf essential oil yield at full flowering stage. The highest amount of essential oil (1.58%) was observed under moderate stress conditions. Poultry manure and vermicompost significantly increased the amount of nitrogen, protein, and percentage of DPPH radical scavenging of leaves under low water stress compared to without stress conditions. The highest yield of essential oil (32.34 kg ha-1), stem dry weight (852.53 kg ha-1), leaf dry weight (1010.40 kg ha-1), and aerial parts dry weight (1863.93 kg ha-1) was obtained in the poultry manure treatment under optimal irrigation. Overall, organic fertilizers application under low water stress could be recommended to increase the plant yield and achieve sustainable agriculture.
