Iranian Journal of Medicinal and Aromatic Plants Research

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Satureja bachtiarica micropropagation through somatic tissues: mass propagation and adaptation

Document Type : Research Paper

Authors

1 Ph.D. student, Department Plant Genetics and Breeding, College of Agriculture, Tarbiat Modares University, Tehran, Iran

2 Department of Plant Genetics and Breeding, College of Agriculture, Tarbiat Modares University, Tehran, Iran

3 Department of Agricultural Biotechnology, College of Agriculture, Tarbiat Modares University, Tehran, Iran

Abstract

Satureja bachtiarica Bunge is one of the endemic Satureja species in Iran, which has strong antioxidant and antimicrobial properties as well as economic and medicinal vlaue due to the presence of compounds like carvacrol and thymol in the essential oil and rosmarinic acid and other phenolic acids in the extract. In this study, 10 shooting and 8 rooting treatments were used in MS medium to produce clones of this species using micropropagation method. The shooting treatments were a combination of cytokinins (Kin, 2ip, and TDZ) and auxins (IBA and BA) with different concentrations. The rooting treatments were also a combination of different concentrations of auxins (IBA and NAA). The best shooting treatment [IBA (0.01 mg l-1), TDZ (0.05 mg l-1), and 2ip (0.3 mg l-1)] was recognized by examining traits such as number and length of main and secondary shoots, number of leaves, number of lateral active buds, and leaf colour. Number of main and lateral roots were also criteria for the best rooting treatment [IBA (0.5 mg l-1) and NAA (0.5 mg l-1)] selection. Since S. bachtiarica is an allogamous plant and has a high diversity for different traits like metabolites, practical purpose of the present study was mass production of identical individuals for use in breeding programs. This purpose was done by presenting the micropropagation protocol of this valuable plant.

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References
- Arrebola, M.L., Socorro, O., Barcelo-Munoz, A., Simon-Perez, E. and Plielgo-Alfaro, F., 1997. Micropropagation of Satureja obovata Lag. HortScience, 32(7): 1278-1280.
- Bairu, M.W., Kulkarni, M.G., Street, R., Mulaudzi, B. and Van Staden, J., 2009. Studies on seed germination, seedling growth, and in vitro shoot induction of Aloe ferox Mill., a commercially important species. HortScience, 44(3): 751-756.
- Echeverrigaray, S., Carrer, R.P. and Andrade, L.B., 2010. Micropropagation of Salvia guaranitica Benth. through axillary shoot proliferation. Brazilian Archives of Biology and Technology, 53: 883-888.
- Jamzad, Z., 2009. Thymus and Satureja species of Iran. Research Institute of Forest and Rangelands publication, 171p.
- Kuris, A., Atlaman, A. and Putievsky, E., 1980. Rooting and initial establishment of stem cuttings of oregano, pepermint and balm. Scientia Horticulturae, 13(1): 53-59.
- Nobre, J., 1996. In vitro cloning and micropropagation of Lavandula Stoechas from field-grown plants. Plant Cell, Tissue and Organ Culture, 46(2): 151-155.
- Ramak, P., Sharifi, M., Kazempour Osaloo, S., Ebrahimzadeh, H. and Behmanesh, M., 2011. Studies on seed germination and in vitro shoot multiplication of Satureja khuzistanica Jamzad, an important medicinal plant. African Journal of Biotechnology, 10(83): 19407-19414.
- Sarihan, E.O., Ipek, A. and Arslan, N., 2003. The effect of indole butyric acid (IBA) on rooting of cuttings from oregano (Origanum vulgare var. hirtum). Field Crops Congress of Turkey, Diyarbakır: 367-372.
- Sefidkon, F., Jamzad, Z. and Barazandeh, M.M., 2005. Essential oil of Satureja bachtiarica Bunge, a potential source of carvacrol. Iranian Journal of Medicinal and Aromatic Plants, 20(4): 425-439.
- Sefidkon, F., Sadeghzadeh, L., Teimouri, M., Asgari, F. and Ahmadi, S.H., 2007. Antimicrobial effects of the essential oils of two Satureja species (S. khuzistanica Jamzad and S. bachtiarica Bunge) in two harvesting time. Iranian Journal of Medicinal and Aromatic Plants, 23(2): 174-181.
- Shariat, A. and Sefidkon, F., 2020. Investigation of pollination system and self-incompatibility in two species of Satureja sahendica and S. bachtiarica. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 27(2): 204-215.
- Shariat, A. and Sefidkon, F., 2021. Tetraploid induction in savory (Satureja khuzistanica): cytological, morphological, phytochemical and physiological changes. Plant Cell, Tissue and Organ Culture, 146: 137-148.
- Shariat, A., Karimzadeh, G. and Assareh, M.H., 2013. Karyology of Iranian endemic Satureja (Lamiaceae) species. Cytologa, 78(3): 305-312.
- Shariat, A., Karimzadeh, G., Assareh, M.H. and Esfahan, E.Z., 2016. Drought stress in Iranian endemic savory (Satureja rechingeri): in vivo and in vitro studies. Plant Physiology and Breeding, 6(1): 1-13.
- Shariat, A., Karimzadeh, G., Assareh, M.H. and Hadian, J., 2017. Variations of physiological indices and metabolite profiling in Satureja khuzistanica in response of drought stress. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 25(2): 174-192.
- Shariat, A., Karimzadeh, G., Assareh, M.H. and Hadian, J., 2018a. A promising application of drought stress for increasing product quality of Iranian endemic Satureja sahendica Bornm, medicinal plant. Iranian Journal of Field Crop Science, 49(1): 167-177.
- Shariat, A., Karimzadeh, G., Assareh, M.H. and Hadian, J., 2018b. Metabolite profiling and molecular responses in a drought-tolerant savory, Satureja rechingeri exposed to water deficit. 3 Biotech, 8(11): 477.
- Shariat, A., Karimzadeh, G., Assareh, M.H. and Loureiro, J., 2018c. Relationships between genome size, ecological and morphological traits in five satureja (Lamiaceae) species. Iranian Journal of Botany, 24(2): 163-173.
- Zimmerman, R.H., Hackett, W.P. and Pharis, R.P., 1985. Hormonal aspects of phase change and precocious flowering. Encyclopedia of Plant Physiology, 11: 79-115.
Iranian Journal of Medicinal and Aromatic Plants Research
Volume 38, Issue 4 - Serial Number 114
December 2022
Pages 581-591
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