Document Type : Research Paper
Authors
1 Ph.D. student, Department of Natural Resources, Isfahan University of Technology, Isfahan, Iran
2 Department of Natural Resources, Isfahan University of Technology, Isfahan, Iran
3 Department of Biology, Faculty of Science, Bu- Ali Sina University, Hamedan, Iran
Abstract
The Salvia species are of great importance in food, pharmaceutical, and cosmetic industries for their secondary metabolites. Although the production of secondary metabolites is encoded by the plant genetic, however, environmental factors have a great influence on their production and composition in plants. In this study, to investigate the effects of some climatic factors, topography, and soil parameters on Salvia multicaulis essential oil composition, the plant samples were collected from nine different habitats in Hamadan province during the flowering season. The soil samples were also taken for each plant in the sampling sites. The essential oil was extracted by hydrodistillation (Clevenger apparatus) and its composition was identified by GC and GC/MS. The soil factors were measured according to the relevant protocols and the climatic records were collected from the meteorological stations of Hamadan province. The RDA analysis was used to investigate the effects of different environmental factors on the essential oil composition. Clustering of the studied habitats was performed based on the major compounds of essential oils using the Wards method. The essential oil composition was different in different habitats. The compounds α-pinene (1.3-32.1%), α-phellandrene (1.1-7%), 1,8-cineole (1-17.2%), bornyl acetate (7-16.2%), and β-caryophyllene (9.1-40.6%) were the major ones of this plant essential oil. The RDA results showed that the essential oil content and oxygenated terpenoids percentage increased with increasing altitude, but the hydrocarbon terpenes percentage had an inverse correlation with altitude. The monoterpenes had a positive correlation with the relative humidity, rainfall, and soil organic carbon, but the sesquiterpenes had an inverse relationship with these factors and showed a positive correlation with phosphorus and to some extent with calcium of the soil. The studied habitats clustered into two distinct groups with predominance of monoterpenes and sesquiterpenes in the essential oil. These findings could be important for commercial exploitation of sage with the aim of extracting specific secondary metabolites in its essential oil.
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Main Subjects
- Adams, R.P., 2007. Review of Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry. Allured Publishing Corporation, Carol Stream, Illinois, 804p.
- Aghaei Joubani, K., Taei, N., Kanani, M.R. and yazdani, M., 2015. Effect of salt stress on some physiological and biochemical parameters of two Salvia species. Journal of Plant Process and Function, 3: 85-96.
- Ahmadi, L. and Mirza, M., 1999. Essential oil of Salvia multicaulis Vahl. from Iran. Journal of Essential Oil Research, 11: 289-290.
- Ahmed, M., Ting, I.P. and Scora, R.W., 1994. Leaf oil composition of Salvia hispanica L. from three geographical areas. Journal of Essential Oil Research, 6(3): 223-228.
- Aissi, O., Boussaid, M. and Messaoud, C., 2016. Essential oil composition in natural populations of Pistacia lentiscus L. from Tunisia: Effect of ecological factors and incidence on antioxidant and antiacetylcholinesterase activities. Industrial Crops and Products, 91: 56-65.
- Ale Omrani Nejad, S.M.H., Naghdi Badi, H., Mehrafarin, A., Abdossi, V. and Khalighi-Sigaroodi, F., 2019. The impact of macro environmental factors on essential oils of Oliveria decumbens Vent. from different regions of Iran. Jundishapur Journal of Natural Pharmaceutical Products, 14(2): e59456.
- Alimohammadi, M., Yadegari, M. and Shirmardi, H., 2017. Effect of elevation and phenological stages on essential oil composition of Stachys. Turkish Journal of Biochemistry, 42(6): 647-656.
- Alonso-Amelot, M.E., Oliveros-Bastidas, A. and Calcagno-Pisarelli, M.P., 2007. Phenolics and condensed tannins of high altitude Pteridium arachnoideum in relation to sunlight exposure, elevation, and rain regime. Biochemical Systematics and Ecology, 35: 1-10.
- Amiri, H., 2012. Chemical composition and antioxidant activity of the essential oil and methanolic extract of Salvia multicaulis Vahl. Journal of Medicinal Plants, 11(41): 111-117.
- Amorati, R., Foti, M.C. and Valgimigli, L., 2013. Antioxidant activity of essential oils. Journal of Agricultural and Food Chemistry, 61(46): 10835-10847.
- Andrade, E.H.A., Alves, C.N., Guimarães, E.F., Carreira, L.M.M. and Maia, J.G.S., 2011. Variability in essential oil composition of Piper dilatatum L.C. Rich. Biochemical Systematics and Ecology, 39: 669-675.
- Ashraf, M. and Orooj, A., 2006. Salt stress effects on growth, ion accumulation and seed oil concentration in an arid zone traditional medicinal plant ajwain (Trachyspermum ammi [L.] Sprague). Journal of Arid Environments, 64: 209-220.
- Ashraf, M., Rehman, S. and Rha, E., 2004. Salt-induced changes in photosynthetic activity and growth in a potential medicinal plant Bishop’s weed (Ammi majus L.). Photosynthetica, 42: 543-550.
- Azarnivand, H. and Zare Chahouki, M.A., 2014. Rangeland Ecology. University of Tehran Press, 338p.
- Azizi, A., Yan, F. and Honermeier, B., 2009. Herbage yield, essential oil content and composition of three oregano (Origanum vulgare L.) populations as affected by soil moisture regimes and nitrogen supply. Industrial Crops and Products, 29: 554-561.
- Bagheri, F. and Yadegari, M., 2021. Properties of essential oil of Salvia syriaca L. under different phenological stages and climatic conditions in Chaharmahal and Bakhtiari province. Journal of Iranian Plant Ecophysiological Research, 16: 94-105.
- Bahi, A., Al Mansouri, S., Al Memari, E., Al Ameri, M., Nurulain, S.M. and Ojha, S., 2014. β-Caryophyllene, a CB2 receptor agonist produces multiple behavioral changes relevant to anxiety and depression in mice. Physiology & Behavior, 135: 119-124.
- Basha, R.H. and Sankaranarayanan, C., 2014. β-Caryophyllene, a natural sesquiterpene, modulates carbohydrate metabolism in streptozotocin-induced diabetic rats. Acta Histochemica, 116(8): 1469-1479.
- Bauer, K., Garbe, D. and Surburg, H., 2001. Common Fragrance and Flavour Materials: Preparation, Properties and Uses. Wiley-VCH, Weinheim, Germany, 293p.
- Ben Jemia, M., Tundis, R., Pugliese, A., Menichini, F., Senatore, F., Bruno, M., Kchouk, M.E. and Loizzo, M.R., 2015. Effect of bioclimatic area on the composition and bioactivity of Tunisian Rosmarinus officinalis essential oils. Natural Product Research, 29: 213-222.
- Burney, O.T., Davis, A.S. and Jacobs, D.F., 2012. Phenology of foliar and volatile terpenoid production for Thuja plicata families under differential nutrient availability. Environmental and Experimental Botany, 77: 44-52.
- Busatta, C., Mossi, A., Rodrigues, M.R., Cansian, R. and Oliveira, J., 2007. Evaluation of Origanum vulgare essential oil as antimicrobial agent in sausage. Brazilian Journal of Microbiology, 38: 610-616.
- Carta, C., Moretti, M.D.L. and Peana, A.T., 1996. Activity of the oil of Salvia officinalis L. against Botrytis cinerea. Journal of Essential Oil Research, 8: 399-404.
- Chang, H.J., Kim, J.M., Lee, J.C., Kim, W.K. and Chun, H.S., 2013. Protective effect of β-caryophyllene, a natural bicyclic sesquiterpene, against cerebral ischemic injury. Journal of Medicinal Food, 16: 471-480.
- Cheng, Y., Dong, Z. and Liu, S., 2014. β-Caryophyllene ameliorates the Alzheimer-like phenotype in APP/PS1 Mice through CB2 receptor activation and the PPARγ pathway. Pharmacology, 94: 1-12.
- Cuvelier, M.E., Richard, H. and Berset, C., 1996. Antioxidative activity and phenolic composition of pilot-plant and commercial extracts of sage and rosemary. Journal of the American Oil Chemists' Society, 73: 645-652.
- Davies, N.W., 1990. Gas chromatographic retention indices of monoterpenes and sesquiterpenes on methyl silicon and carbowax 20m phases. Journal of Chromatography A, 503: 1-24.
- Dehghan, Z., Sefidkon, F., Emami, S.M. and Kalvandi, R., 2014. The effects of ecological factors on essential oil yield and composition of Ziziphora clinopodioides Lam. subsp. rigida (Boiss.) Rech.f. Journal of Plant Research, 27: 61-71.
- Demirkaya, E., Acikel, C., Hashkes, P., Gattorno, M., Gul, A., Ozdogan, H., Turker, T., Karadag, O., Livneh, A., Ben-Chetrit, E. and Ozen, S., 2016. Development and initial validation of international severity scoring system for familial Mediterranean fever (ISSF). Annals of the Rheumatic Diseases, 75(6): 1051-1056.
- Dorman, H.J.D. and Deans, S.G., 2000. Antimicrobial agents from plants: antibacterial activity of plant volatile oils. Journal of Applied Microbiology, 88(2): 308-316.
- El-Keltawi, N.E. and Croteau, R., 1987. Salinity depression of growth and essential oil formation in spearmint and marjoram and its reverse foliar applied cytokinin. Phytochemistry, 26(5): 1333-1334.
- Falco, E., Mancini, E., Roscigno, G., Mignola, E., Taglialatela-Scafati, O. and Senatore, F., 2013. Chemical composition and biological activity of essential oils of Origanum vulgare L. subsp vulgare L. under different growth conditions. Molecules, 18(12): 14948-14960.
- Fernández-Sestelo, M. and Carrillo, J.M., 2020. Environmental effects on yield and composition of essential oil in wild populations of spike lavender (Lavandula latifolia Medik.). Agriculture, 10(12): 626.
- Figueiredo, A.C., Barroso, J.G., Pedro, L.G. and Scheffer, J.J.C., 2008. Factors affecting secondary metabolite production in plants: volatile components and essential oils. Flavour and Fragrance Journal, 23(4): 213-226.
- Ghahraman, A., 1988. Color Flora Iran. Iranian Research Institute of Rangelands and Forests.
- Gholami, B., 2013. Secondary metabolites of plants and their possible biological application in agricultural ecosystems. Proceeding of third National Conference on the Development of Application of Biological Materials and Optimal Use of Fertilizer and Poison, Agricultural Research Institute of Seed and Plant Improvement, Karaj, 790p.
- Graven, E.H., Webber, L., Venter, M. and Gardner, J.B., 1990. The development of Artemisia afra (Jacq.) as a new essential oil crop. Journal of Essential Oil Research, 2: 215-220.
- Guetat, A., 2014. 1,8-Cineole, α-pinene and verbenone chemotype of essential oil of species Rosmarinus officinalis L. from Saudi Arabia. International Journal of Herbal Medicine, 2(2): 137-141.
- Habibi, H., Mazaheri, D., Majnoun Hosseini, N., Chaeichi, M.R., Fakhr Tabatabaei, M. and Bigdeli, M., 2007. Effect of altitude on essential oil and components in wild thyme (Thymus kotschyanus Boiss.) Taleghan region. Pajouhesh-va-Sazandegi, 19: 2-10.
- Hamidpour, R., Hamidpour, S., Hamidpour, M. and Shahlari, M., 2013. Sage: The functional novel natural medicine for preventing and curing chronic illnesses. International Journal of Case Reports and Images, 4(12): 671-677.
- Hemati, K.H., Hemati, N. and Ghaedi, A., 2015. The effect of habitat, root diameter, and type of tissue on some secondary metabolites content of licorice (Glycerrhiza glabra) in Khorasan Razavi (Ghoochan). Journal of Plant Environmental Physiology, 10(3): 1-10.
- Hosseinzadegan, R. and Bakhshi Khaniki, G., 2013. Investigation of chemical composition of essential oil of Teucrium polium l. in different habitats of Mazandaran province. New Cellular & Molecular Biotechnology Journal, 3: 47-55.
- Jafarian, Z., Goldansaz, M., Safaeeian, R., Sonboli, A. and Kargar, M., 2020. The effect of environmental factorss on the amount of essential oil of Nepeta asterotricha Rech.f. using RDA technique. Desert Management, 7(14): 167-180.
- Jalas, J., 1982. Thymus, Flora of Turkey and East Aegean Island. Edinburgh University Press, Edinburgh.
- Jassbi, A.R., Asadollahi, M., Masroor, M., Schuman, M.C., Mehdizadeh, Z., Soleimani, M. and Miri, R., 2012. Chemical classification of the essential oils of the iranian Salvia species in comparison with their botanical taxonomy. Chemistry & Biodiversity, 9(7): 1254-1271.
- Kamatou, G.P.P., Viljoen, A.M. and Steenkamp, P., 2010. Antioxidant, antiinflammatory activities and HPLC analysis of South African Salvia species. Food Chemistry, 119: 684-688.
- Karami, M., Hossini, E., Shahbi Majd, N., Ebrahimzadeh, M.A. and Alemy, S., 2015. Salvia limbata: Botanical, chemical, pharmacological and therapeutic effecte. Clinical Excellence, 3(2): 1-14.
- Karousou, R., Koureas, D.N. and Kokkini, S., 2005. Essential oil composition is related to the natural habitats: Coridothymus capitatus and Satureja thymbra in NATURA 2000 sites of Crete. Phytochemistry, 66(22): 2668-2673.
- Kim, M.K., Park, G.H., Eo, H.J., Song, H.M., Lee, J.W., Kwon, M.J., Koo, J.S. and Jeong, J.B., 2015. Tanshinone I induces cyclin D1 proteasomal degradation in an ERK1/2 dependent way in human colorectal cancer cells. Fitoterapia, 101: 162-168.
- Kintzios, S.E., 2006. Terrestrial plant-derived anticancer agents and plant species used in anticancer research. Critical Reviews in Plant Sciences, 25(2): 79-113.
- Konare, H., Yost, R., Doumbia, M., McCarty, G., Jarju, A. and Kablan, R., 2010. Loss on ignition: Measuring soil organic carbon in soils of the Sahel, West Africa. African Journal of Agricultural Research, 5(22): 3088-3095.
- Kubelka, W., 2011. Teedrogen und Phytopharmaka. Scientia Pharmaceutica, 79: 703-703.
- Legault, J. and Pichette, A., 2008. Potentiating effect of beta-caryophyllene on anticancer activity of alpha-humulene, isocaryophyllene and paclitaxel. The Journal of Pharmacy and Pharmacology, 59(12): 1643-1647.
- Leicach, S.R. and Chludil, H., 2014. Plant secondary metabolites: Structure-activity relationships in human health prevention and treatment of common diseases. Studies in Natural Products Chemistry, 42: 267-304.
- Li, L., Li, Z.W., Yin, Z.Q., Wei, Q., Jia, R.Y., Zhou, L.J. and Xu, J., 2014. Antibacterial activity of leaf essential oil and its constituents from Cinnamomum longepaniculatum. International Journal of Clinical and Experimental Medicine, 7(7): 1721-1727.
- Lima, D.F., Brandão, M.S., Moura, J.B., Leitão, J.M., Carvalho, F.A., Miúra, L.M. and Leite, J.R., 2012. Antinociceptive activity of the monoterpene α-phellandrene in rodents: possible mechanisms of action. Journal of Pharmacy and Pharmacology, 64(2): 283-292.
- Lin, J.J., Hsu, S.C., Lu, K.W., Ma, Y.S., Wu, C.C., Lu, H.F. and Chen, J.C., 2016. Alpha‐phellandrene‐induced apoptosis in mice leukemia WEHI-3 cells in vitro. Environmental Toxicology, 31(11): 1640-1651.
- Mahajan, M., Kuiry, R. and Pal, P.K., 2020. Understanding the consequence of environmental stress for accumulation of secondary metabolites in medicinal and aromatic plants. Journal of Applied Research on Medicinal and Aromatic Plants, 18: 100255.
- Mahzooni-Kachapi, S.S., Mahdavi, M., Jouri, M., Akbarzadeh, M. and Rouzbeh, L., 2014. The effects of altitude on chemical compositions and function of essential oils in Stachys lavandulifolia Vahl. (Iran). International Journal of Medicinal and Aromatic Plants, 4: 107-116.
- Maxwell, A. and Jones, P., 2007. Factors Affecting the Growth and Secondary Metabolism of Medicinal Plants. North Mississippi Research and Extension Center 5421 S Hwy 145, Verona, MS 38879.
- Miguel, M.G., 2010. Antioxidant and anti-inflammatory activities of essential oils: a short review. Molecules, 15(12): 9252-9287.
- Miladinović, D.L., Ilić, B.S. and Kocić, B.D., 2015. Chemoinformatics approach to antibacterial studies of essential oils. Natural Product Communications, 10(6): 1063-1066.
- Milos, M., Radonic, A., Bezic, N. and Dunkic, V., 2001. Localities and seasonal variations in the chemical composition of essential oils of Satureja montana L. and S. cuneifolia Ten. Flavour and Fragrance Journal, 16: 157-160.
- Mir-Azadi, Z., Pilehvar, B. and Tajalli, A.A., 2013. Description of some ecological factors in three forest sites in lorestan province and their impact on myrtle (Myrtus communis L.) essential oil yield and chemical components. Journal of Crop Production and Processing, 2(6): 43-52.
- Mohsenpour, M., Vafadar, M., Vatankhah, E. and Meighani, H., 2017. The impact of the environmental factors on yield and chemical compositions of essential oil of water mint, Mentha aquatica L. from different habitats of Mazandaran province. Journal of Plant Research, 30(2): 440-451.
- Moo, C.L., Osman, M.A., Yang, S.K., Yap, W.S., Ismail, S., Lim, S.H.E., Chong, C.M. and Lai, K.S., 2021. Antimicrobial activity and mode of action of 1,8-cineol against carbapenemase-producing Klebsiella pneumoniae. Scientific Reports, 11: 20824.
- Morteza-Semnani, K., Saeedi, M., Changizi, S. and Vosoughi, M., 2005. Essential oil composition of Salvia Virgata Jacq. from Iran. Journal of Essential Oil Bearing Plants, 8: 330-333.
- Nelson, D.W. and Sommers, L.E., 1983. Total carbon, organic carbon, and organic matter. 539-579. In: Page, A.L., (Ed.). Methods of Soil Analysis: Part 2, Chemical and Microbiological Properties. American Society of Agronomy, US, 1159p.
- Oloumi, H. and Hassibi, N. 2012. Study the content of some secondary metabolites in roots of Glycyrrhiza glabra gathered from different natural localities in kerman province of Iran. Journal of Medicinal Plants, 11: 137-144.
- Olsen, S.R., Cole, C.V., Watanabe, F.S. and Dean, L.A., 1954. Estimation of available phosphorus in soils by extraction with sodium bicarbonate. US Dept. of Agriculture, Washington, 19p.
- Omer, B., Krebs, S., Omer, H. and Noor, T.O., 2007. Steroid-sparing effect of wormwood (Artemisia absinthium) in Crohn's disease: a double-blind placebo-controlled study. Phytomedicine, 14: 87-95.
- Ozturk, A., Ünlükara, A., Ipek, A. and Gürbüz, B., 2004. Effects of salt stress and water deficit on plant growth and essential oil content of lemon balm (Melissa officinalis L.). Pakistan Journal of Botany, 36(4): 787-792.
- Pehlivan, M. and Sevindik, M., 2018. Antioxidant and Antimicrobial Activities of Salvia multicaulis. Turkish Journal of Agriculture- Food Science and Technology, 6(5): 628-631.
- Piccaglia, R., Marotti, M. and Dellacecca, V., 1997. Effect of planting density and harvest date on yield and chemical composition of sage oil. Journal of Essential Oil Research, 9: 187-191.
- Piccinelli, A.C., Santos, J.A., Konkiewitz, E.C., Oesterreich, S.A., Formagio, A.S., Croda, J., Ziff, E.B. and Kassuya, C.A., 2015. Antihyperalgesic and antidepressive actions of (R)-(+)-limonene, α-phellandrene, and essential oil from Schinus terebinthifolius fruits in a neuropathic pain model. Nutritional Neuroscience, 18(5): 217-224.
- Pitarokili, D., Couladis, M., Petsikos-Panayotarou, N. and Tzakou, O., 2002. Composition and antifungal activity on soil-borne pathogens of the essential oil of Salvia sclarea from Greece. Journal of Agricultural and Food Chemistry, 50(23): 6688-6691.
- Rabib, H., Elagdi, C., Hsaine, M., Fougrach, H., Koussa, T. and Badri, W., 2020. Antioxidant and antibacterial activities of the essential oil of Moroccan (Tetraclinis articulata Vahl.) masters. Biochemistry Research International, 2020: 9638548.
- Rajeswara Rao, B.R., Sastry, K.P., Prakasa Rao, E.V.S. and Ramesh, S.I., 1990. Variation in yields and quality of geranium (Pelargonium graveolens L' Hér. ex Aiton) under varied climatic and fertility conditions. Journal of Essential Oil Research, 2(2): 73-79.
- Rashmi, B.N., Harlapur, S.F., Avinash, B., Ravikumar, C.R., Nagaswarupa, H.P., Anil Kumar, M.R., Gurushantha, K. and Santosh, M.S., 2020. Facile green synthesis of silver oxide nanoparticles and their electrochemical, photocatalytic and biological studies. Inorganic Chemistry Communications, 111: 107580.
- Rhoades, J.D., 1996. Salinity: electrical conductivity and total dissolved solids: 417-435. In: Sparks, D.L., Page, A.L., Helmke, R.H., Loeppert, P.N., Soltanpour, M.A., Tabatabai, C.T., Johnston, M.E. and Sumner, E., (Eds.). Methods of Soil Analysis: Part 3 Chemical Methods, John Wiley & Sons, USA, 1424p.
- Rufino, A., Ribeiro, M., Sousa, C.T., Judas, F., Salgueiro, L., Cavaleiro, C. and Mendes, A., 2015. Evaluation of the anti-inflammatory, anti-catabolic and pro-anabolic effects of E-caryophyllene, myrcene and limonene in a cell model of osteoarthritis. European Journal of Pharmacology, 750:141-150.
- Sardi, J.C.O., Scorzoni, L., Bernardi, T., Fusco-Almeida, A.M. and Mendes Giannini, M.J.S., 2013. Candida species: current epidemiology, pathogenicity, biofilm formation, natural antifungal products and new therapeutic options. Journal of Medical Microbiology, 62: 10-24.
- Schauer, N., Steinhauser, D., Strelkov, S., Schomburg, D., Allison, G., Moritz, T. and Lundgren, K., 2005. GC-MS libraries for the rapid identification of metabolites in complex biological samples. FEBS Letters, 579(6): 1332-1337.
- Sefidkon, F., Kalvandi, R., Atri, M. and Barazandeh, M., 2005. Essential oil variability of Thymus eriocalyx (Ronniger) Jalas. Flavour and Fragrance Journal, 20: 521-524.
- Senatore, F., Apostolides, N., Piozzi, F. and Formisano, C., 2006. Chemical composition of the essential oil of Salvia microstegia Boiss. et Balansa growing wild in Lebanon. Journal of Chromatography A, 1108: 276-278.
- Shahriari, S., Barekatain, M., Shahtalebi, M. and Farhad, S., 2019. Evaluation of preventive antibacterial properties of a glass-ionomer cement containing purified powder of Salvia officinalis: an in vitro study. International Journal of Preventive Medicine, 10: 110.
- Sharmeen Jugreet, B., Kouadio Ibrahime, S., Zengin, G., Abdallah, H.H. and Fawzi Mahomoodally, M., 2021. GC/MS profiling, in vitro and in silico pharmacological screening and principal component analysis of essential oils from three exotic and two endemic plants from mauritius. Chemistry & Biodiversity, 18(3): e2000921.
- Singh, R., 2015. Medicinal plants: a review. Journal of Plant Sciences, 3: 50-55.
- Sonboli, A., Kanani, M.R., Yousefzadi, M. and Mojarad, M., 2009. Chemical composition and antibacterial activity of the essential oil of Salvia hydrangea from two localities of Iran. Journal of Medicinal Plants, 8: 20-28.
- Tada, M., Okuno, K., Chiba, K., Ohnishi, E. and Yoshii, T., 1994. Antiviral diterpenes from Salvia officinalis. Phytochemistry, 35(2): 539-541.
- Thomas, G.W., 1996. Soil pH and Soil Acidity: 475-490. In: Sparks, D.L., Page, A.L., Helmke, R.H., Loeppert, P.N., Soltanpour, M.A., Tabatabai,C.T., Johnston, M.E. and Sumner, E., (Eds.). Methods of Soil Analysis: Part 3 Chemical Methods, John Wiley & Sons, USA, 1424p.
- Tommasi, L., Negro, C., Cerfeda, A., Nutricati, E., Zuccarello, V., De Bellis, L. and Miceli, A., 2007. Influence of environmental factors on essential oil variability in Thymbra capitata (L.) Cav. growing wild in Southern Puglia (Italy). Journal of Essential Oil Research, 19: 572-580.
- Verma, N. and Shukla, S., 2015. Impact of various factors responsible for fluctuation in plant secondary metabolites. Journal of Applied Research on Medicinal and Aromatic Plants, 2: 105-113.
- Viljoen, A.M., Petkar, S., van Vuuren, S.F., Figueiredo, A.C., Pedro, L.G. and Barroso, J.G., 2006. The chemo-geographical variation in essential oil composition and the antimicrobial properties of “Wild Mint” - Mentha longifolia subsp. polyadena (Lamiaceae) in Southern Africa. Journal of Essential Oil Research, 18: 60-65.
- Villa, C., 2018. Green Cosmetic Ingredients and Processes: 303-330. In: Salvador, A. and Chisvert, A., (Eds.). Analysis of Cosmetic Products. Elsevier, Boston. 630p.
- Walker, J., Sytsma, K., Treutlein, J. and Wink, M., 2004. Salvia (Lamiaceae) is not monophyletic: Implications for the systematics, radiation, and ecological specializations of Salvia and tribe Mentheae. American Journal of Botany, 91(7): 1115-1125.
- Wu, X., Xiao, F., Zhang, Z., Li, X. and Xu, Z., 2005. Research on the analgesic effect and mechanism of bornyl acetate in volatile oil from amomum villosum. Zhong Yao Cai, 28: 505-507.
- Wu, Y.B., Ni, Z.Y., Qing, S., Dong, M., Kiyota, H., Gu, Y.C. and Cong, B., 2012. Constituents from Salvia species and their biological activities. Chemical Reviews, 11: 5967-6026.
- Yang, H., Zhao, R., Chen, H., Jia, P., Bao, L. and Tang, H., 2014. Bornyl acetate has an anti-inflammatory effect in human chondrocytes via induction of IL-11. IUBMB Life, 66(12): 854-859.
- Yang, L., Wen, K.S., Ruan, X., Zhao, Y.X., Wei, F. and Wang, Q., 2018. Response of plant secondary metabolites to environmental factors. Molecules, 23(4): 762.
- Yavari, A., Nazeri, V., Sefidkon, F. and Hassani, M.E., 2010. Influence of some environmental factors on the essential oil variability of Thymus migricus. Natural Product Communications, 5(6): 943-948.
- Yazdinezhad, A. and Malekzadeh, M., 2015. Evaluation of antioxidant effect, total phenols, anthocyanins and flavonoids contents of methanolic extract of Salvia viridis L. collected from Zanjan name and address. Journal of Advances in Medical and Biomedical Research, 23: 100-108.
- Yeddes, W., Aidi Wannes, W., Hammami, M., Smida, M., Chebbi, A., Marzouk, B. and Saidani Tounsi, M., 2018. Effect of environmental conditions on the chemical composition and antioxidant activity of essential oils from Rosmarinus officinalis L. growing wild in Tunisia. Journal of Essential Oil Bearing Plants, 21: 972-986.
- Yousefzadi, M., Sonboli, A., Nejad Ebrahimi, S. and Hashemi, S., 2008. antimicrobial activity of essential oil and major constituents of Salvia chloroleuca. Zeitschrift für Naturforschung C, 63: 337-340.
- Zaouali, Y., Bouzaine, T. and Boussaid, M., 2010. Essential oils composition in two Rosmarinus officinalis L. varieties and incidence for antimicrobial and antioxidant activities. Food and Chemical Toxicology, 48: 3144-3152.
- Zhang, J.H., Sun, H.L., Chen, S.Y., Zeng, L. and Wang, T.T., 2017. Anti-fungal activity, mechanism studies on α-Phellandrene and Nonanal against Penicillium cyclopium. Botanical Studies, 58: 13.
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