Biotechnology
M. Yahyazadeh; N. Hadi; Z. Shirazi; K. Jaimand; Kh. Karimzadeh Asl; M. Makizadeh Tafti; S. Fekri Qomi; M. Rahimifard; M. Gorji; F. Askari; Z. Behrad; D. Selmar
Abstract
Plants are the main sources of secondary metabolites with high medical value. The most important member of these valuable compounds are alkaloids with the different drug purposes. Concerning the limited production of some of these metabolites in the plants, these medicinal compounds can be produced naturally ...
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Plants are the main sources of secondary metabolites with high medical value. The most important member of these valuable compounds are alkaloids with the different drug purposes. Concerning the limited production of some of these metabolites in the plants, these medicinal compounds can be produced naturally and commercially with the identification and transfer of alkaloids-producing enzymes corresponding plant genes to the microorganisms as an alternative method. In this way, the characterization of the corresponding genes is the first step. Among the different enzymes involved in the alkaloid biosynthesis, the cytochrome P450 enzymes play an important role. Due to the endoplasmic reticulum (ER) localization of these enzymes and their glycoprotein characters, they cannot be expressed functionally in the standard bacterial systems. Consequently, the heterologous expression aimed to verify the enzymatic activity can favorably be performed using the eukaryotic systems, like yeast or insect cells. Herein, in this study, with employing a phylogenic comparison of cheilanthifoline synthase sequence of Eschscholzia californica Cham. and comparing the sequence with the homolog amino acid sequences of Chelidonium majus L. achieved from bioinformatics databases, six cytochrome P450 enzymes responsible for cheilanthifoline synthase in Ch. majus were identified. To prove the efficacy of these enzymes practically, their genes were cloned into the pPIC3.5 vector. Then, these recombinant vectors were transferred to the yeast cell (Pichia pastoris) and the scoulerine alkaloid was given to its media. Finally, the cheilanthifoline alkaloid microbial production by P. pastoris containing the recombinant plasmids was evaluated by LC-MS. The results of the present study indicated that among the enzymes genes cloned and introduced to the yeast host, only the Contig8931 enzyme had the cheilanthifoline synthase activity.
A. Kamaraki Farahani; P. Baghaee; M.B. Rezaee; K. Jaimand
Volume 20, Issue 4 , January 2018, , Pages 407-416
Abstract
Saffron spice is the dried, dark- red stigmata of crocus sativus. L flowers is a very high valve material due to its applications, mainly those to provide color and flavor to foods . Dietary factors play a significant role in both cancer promotion and prevention. Carotenoieds are one of the most common ...
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Saffron spice is the dried, dark- red stigmata of crocus sativus. L flowers is a very high valve material due to its applications, mainly those to provide color and flavor to foods . Dietary factors play a significant role in both cancer promotion and prevention. Carotenoieds are one of the most common dietary compounds that have been studied as cancer- preventive agents. Recently extracts from natural products and saffron ave also been shown to exhibit anticancer activity.the main pigments of saffron are mono – and di- glycosyle esters of the polyene dicarboxylic acid crocetin, where D-glycose and D- gentobiose occurs as carbohydrate residues.For the extracton of saffron 20 gr was successively and exhausively extracted with light petroleum , diethyl ether and methanol in a soxhlet extractor in the dark. The methanol extract contained the glycosede carotenoids of saffron. Separation of the constituents was achieved by silica gel TLC using diffrents solvent (mobile phase) system. The TLC method was time consuming and also gave an overestimation of the colour princiles.
M.H. Asareh; M.M. Barazandeh; K. Jaimand
Volume 20, Issue 4 , January 2018, , Pages 469-476
Abstract
Eucalyptus porosa was collected from Dezfool (Fadak Garden) in Martch 2004.Dry leaves of it were hydrodistilled in a clevenger type apparatus for 20 min. to produce an oil at the yield of 0.57%(based on dry leaves).In order of quantitavely and qualitavely analyses, the oil was injected to GC.Among Twenty-one ...
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Eucalyptus porosa was collected from Dezfool (Fadak Garden) in Martch 2004.Dry leaves of it were hydrodistilled in a clevenger type apparatus for 20 min. to produce an oil at the yield of 0.57%(based on dry leaves).In order of quantitavely and qualitavely analyses, the oil was injected to GC.Among Twenty-one compounds which were identified in the oil, 1,8-cineole (58.6%), α–pinene(12.8%) and nopinone (3.11%) were the major constituents, respectively.
S.R. Tabaei-Aghdaei; M.B. Rezaee; K. Jaimand
Volume 20, Issue 4 , January 2018, , Pages 533-545
Abstract
Rosa damascene Mill. wascollected from west parts of Iran, and cultivated at the experimental field of Research Institute of Forests and Rangelands, using a three replicated randomized complete blocks design. Seven genotypes, were compared for essential oils yield in 2003 and for petal, stamen, ...
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Rosa damascene Mill. wascollected from west parts of Iran, and cultivated at the experimental field of Research Institute of Forests and Rangelands, using a three replicated randomized complete blocks design. Seven genotypes, were compared for essential oils yield in 2003 and for petal, stamen, and pistil number, in 2003-2004. Genotypes showed significant differences in terms of the measured traits. Comparison of means classified the genotypes in 7, 7 and 7 (in 2003) and 6, 7 and 6 (in 2004) groups, for petal, stamen and pistil number, respectively. Also, essential oils concentration and yield showed genotypic variation. A considerable variation was, therefore, revealed from different analyses in floral parts and essential oils concentration of Rosa damascena Mill. from west parts of Iran. It could then be concluded from the results of different analyses that although the genotypes could be grouped based on geographical origins, concentration and yield of essential oils as the most important product of Rosa damascena Mill., and also some of the related traits, such as floral parts could be used as appropriate selection factors for classification of damask rose genotypes, in order to use in breeding program.
M. Shahi; M. Saghari; E. Zandi Esfahan; K. Jaimand
Abstract
Given the extent of saline lands in Iran, cultivation and utilization of halophytes and salt tolerant species under the condition that both water and soil are saline could be a viable option in production and extraction of vegetable oils from halophytes and salt tolerant species. The aim of this study ...
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Given the extent of saline lands in Iran, cultivation and utilization of halophytes and salt tolerant species under the condition that both water and soil are saline could be a viable option in production and extraction of vegetable oils from halophytes and salt tolerant species. The aim of this study was to investigate the potential of Suaeda fruticosa (L.) Forssk. and Salicornia herbacea L. as a source of edible oil as well as qualitative and quantitative analysis of the oil. For this purpose, the seeds of Suaeda fruticosa and Salicornia herbacea were collected from saline soils of Garmsar and Kal-e Shur Desert Research Station. In this study, a random sampling was done by replicated plots. The extraction of fatty acids was performed by solvent in Soxhlet method, and GC was used to analyze the fatty acids. The average oil yield was calculated to be 30.76 and 13.88% in Suaeda fruticosa and Salicornia herbacea, respectively. According to the results, in both species, palmitic acid and linoleic acid were identified as the major saturated and unsaturated fatty acids, respectively. Our data clearly indicate that the seeds of halophyte Suaeda fruticosa could be used as a source of oil for human consumption.
B. Yoosefi; H.R. Ghasempoor; B. Yousefi; S.R. Tabaeii Aghdaie; K. Jaimand
Abstract
Damask Rose (Rosa damascena Mill.) is an important medicinal and industrial species. To achieve more and better-quality essential oil identifying the superior accessions of Damask Rose and their genetic relationships is very important. For a detailed review of the relationship among the genotypes of ...
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Damask Rose (Rosa damascena Mill.) is an important medicinal and industrial species. To achieve more and better-quality essential oil identifying the superior accessions of Damask Rose and their genetic relationships is very important. For a detailed review of the relationship among the genotypes of this species and variations in the essential oil composition, the use of multivariate statistical analysis could be useful. In this study, 25 different accessions of Damask Rose were collected from Kermanshah province and other regions of Iran and were planted in a randomized complete blocks design. The essential oil was extracted from the petals with hydrodistillation method. Essential oil composition was identified by using the gas chromatography and gas chromatography coupled with mass spectrometry and the amount (percentage) of each composition was measured. Essential oil compositions were evaluated using cluster analysis. According to the results of cluster analysis, the accessions were grouped in four groups. Discriminant analysis confirmed the results of cluster analysis. Component analysis showed that the accessions were in four groups and there were four different trends among the accessions studied for the chemical compositions of essential oils. According to the correlation analysis, the two important factors, geraniol and citronellol, which increased the quality of essential oil compounds in damask rose, showed a significant positive relationship with each other and with citronellyl acetate and geranial.
K. Jaimand; M.B. Rezaee; F. Sefidkon; M. Naderi; H. Keneshloo; M.Y. Achak; M. Farahpour; Sh. Karimi
Abstract
Moringa peregrine (Forssk.) Fiori is one of the species growing in tropical and desert areas and is distributed in the southeastern of the country in Hormozghan and Sistan and Balochestan provinces. Moringa peregrine is a desert shrub that can grow in areas with little rainfall, andit has a great nutritional, ...
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Moringa peregrine (Forssk.) Fiori is one of the species growing in tropical and desert areas and is distributed in the southeastern of the country in Hormozghan and Sistan and Balochestan provinces. Moringa peregrine is a desert shrub that can grow in areas with little rainfall, andit has a great nutritional, pharmaceutical, environmental, industrial and economic values. This research was aimed to determination of fatty acids in Moringa peregrina seed oil from different locations in Sistan and Balochestan province in 2009. Five samples were collected from five natural research stations. The oil content was determined by the Soxhlet method. The oil content obtained from Tong Fonoj, Keneshky, Bent, Begaband, and Dorahy Chanf sations were measured to be 54.6%, 50.4%, 52.4%, 53.2%, and 53.6% respectively. According to the obtained results, fatty acids content varied as follows: oleic acid (71.5-74%), palmitic acid (12.6-14.7%), Iso-oleic acid (3.6-4.5%), palmitoleic acid (3.3-4.7%), stearic acid (1.9-2.4%), Behenic acid (0.9-1.5%), gadoleic acid (0.4-10.9%) and Arachidonic acid (0.4-1.3%). Over 90 percent of the oil consumed in the country is imported from abroad. Our results clearly indicate that due to the amount of fatty acids in Moringa peregrina seeds, this plant can be considered as a new source for the production of vegetable oil and widespread food consumption.
A. Nikoupour; K. Jaimand; M.T. Darzi; F. Rejali
Abstract
In order to evaluate the performance of biological phosphorus fertilizer and plant density on the quantity and quality of essential oils in Ajowan (Trachyspermum copticum L.), this research was performed as a two factors factorial experiment in a randomized complete block design with three replications ...
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In order to evaluate the performance of biological phosphorus fertilizer and plant density on the quantity and quality of essential oils in Ajowan (Trachyspermum copticum L.), this research was performed as a two factors factorial experiment in a randomized complete block design with three replications at the research field of the Research Institute of Forests and Rangelands in spring 2010. The treatments consisted of four levels of the phosphorus bio-fertilizer (Control (non-inoculated) seed soaked with liquid fertilizer, soluble fertilizer to the plants and seed soaked with liquid fertilizer & soluble fertilizer to the plants) and three levels of density (250000, 125000, 83000 plants per hectare). The studied characteristics included essential oil percentage, oil yield, percentage of thymol, terpinolene and p-cymene were in volatil oils. Results showed that the effect of bio-fertilizer phosphorus on the studied characteristics were significant at 1% , so that the highest amounts of oil percentage (4.34%) and oil yield (143.12 kg per hectare) were obtained in double inoculation treatment (seed soaked with liquid fertilizer & soluble fertilizer to the plants). In addition, the highest percentages of thymol (33.9%), terpinolene (41.5%), and p-cymene (23.3%) were obtained in the treatments of seed soaked with liquid fertilizer, double inoculation treatment, and soluble fertilizer, respectively. The effect of plant density on the studied characteristics was significant at 1% level of significance, so that the highest amounts of oil percentage (4.1%), oil yield (104.07 kg ha-1), thymol (37.4%), terpinolene (44.5%) and p-cymene (23.9%) were obtained in plant density treatments of 83000, 250000, 125000, and 83000 plants per hectare, respectively. However, significant interaction effects were also observed on oil percentage (5.37%), oil yield (149.5 kg per hectare), percentage of thymol (40%), percentage of terpinolene (49.1%) and percentage of para-cymene (25.6%).
M.H. RAD; K. Jaimand; M.H. Assareh; M. Soltani
Abstract
Drought stress has different effects on morphological, physiological and biochemical processes of plants. Moreover, the quantity and quality of essential oil are affected by drought stress. Eucalyptus species including Eucalyptus camaldulensis Dehnh. are considered as rich sources of essential oils especially ...
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Drought stress has different effects on morphological, physiological and biochemical processes of plants. Moreover, the quantity and quality of essential oil are affected by drought stress. Eucalyptus species including Eucalyptus camaldulensis Dehnh. are considered as rich sources of essential oils especially 1,8-cineol. In order to study the effect of drought stress on essential oil yield, the type of chemical compounds and also water use efficiency in E. camaldulensis, this research was conducted in a completely randomized design with three treatments including 100, 70, and 40% field capacity and three replications. The experiment was carried out in combat desertification research station of Shahid Sadoughi under lisymetry conditions. Results showed that increasing of soil moisture led to the increased production of essential oil. However, mild drought stress resulted in increased essential oil yield and improved water use efficiency. Mild drought stress compared to other treatments (p < 0.001), improved the production of 1, 8-cineol, however it was observed that it decreased or stopped the production of many other compounds. The percentage of 1, 8 - cineol in the treatments of 100, 70 and 40% field capacity, were, 69.33, 78.13 and 51.87, respectively. The production of α-pinene, as another important compound was also affected by enough moisture (without stress) so that It's value showed significant difference with other treatments (p <0.001). The percentage of α-pinene in the treatments of 100, 70 and 40% field capacity, were, 14.0, 7.03 and 4.06, respectively. The moisture treatments including 100% FC (without stress), 70% FC (mild stress) and 40% FC (severe stress) were studied with three replicates and completely randomize design.
K. Jaimand; M.H. Assareh; M. Mirza; M. Nadery; Sh. Karimi; E. Parsa
Abstract
Eucalyptuses trees as the fast-growing forest species are native to the Pacific. This species were introduced to Iran more than 100 years ago and cultivated in the south, having perfect environment for them. This research was aimed to investigate seasonal fluctuations of essential oil and1,8-cineol content ...
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Eucalyptuses trees as the fast-growing forest species are native to the Pacific. This species were introduced to Iran more than 100 years ago and cultivated in the south, having perfect environment for them. This research was aimed to investigate seasonal fluctuations of essential oil and1,8-cineol content in Eucalyptus species of Fars province. The leaves of 14 Eucalyptus species were collected from Fars province in the middle of each season for two years (2007 & 2008). Afterward, essential oils were obtained by hydrodistillation method and compounds were identified by GC and GC/MS. Results showed that in each region, some of the species had the highest essential oil yield and quality; therefore due to the high essential oil content and 1,8-cineol, they could be recommended for large-scale cultivation in the region in order to extract essential oil. Studied Eucalyptus included: E. calcicultrix (Miq.) Blakely; E. camaldulensis var. camaldulensis Dehnh.; E. cornuta; E. fruticetorum; E. intertexta R.T. Baker; E. largiflorens F. Muell.; E. lesouefii; E. longicornis F. Muell.; E. oleosa F. Muell.; E. rudis Endl.; E. salmonophloia F. Muell.; E. salubaris; E. stricklandii Maiden; E. viridis. The essential oil yield of different species at different seasons differed from 1.0 to 6.8% in 2007 and 0.3 to 5.8% in 2008. According to the results, the highest oil yield in autumn (5.2%-6.4%), the highest percentage of 1,8-cineole in winter (91.4%-94%), and the best time to harvest were recorded for E. oleosa F. Muell at Shousni, Mamasani; therefore, E. oleosa F. Muell could be recommended for cultivation as the best species.
K. Jaimand; H. Ahrabi Asli; Z. Behrad
Abstract
This research was aimed to extract and measure the quercetin and kampferol in Foeniculum vulgare Mill. For this purpose, in the end of May 2010, samples were collected from Research Institute of Forests and Rangelands, and then various organs (flowers, leaves, stems, and seeds) were extracted with different ...
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This research was aimed to extract and measure the quercetin and kampferol in Foeniculum vulgare Mill. For this purpose, in the end of May 2010, samples were collected from Research Institute of Forests and Rangelands, and then various organs (flowers, leaves, stems, and seeds) were extracted with different methods. In the first method, samples were extracted with chloroform solvent by Soxhlet for 72 hours. In the second method, after removing the solvent, methanol was added to the previous sample extracted with chloroform solvent and extraction was repeated. In the third method, depending on the amount of dry matter, different organs of fresh fennel were weighted; then they were grounded with the solvents of methanol and acetic acid (ratio 1:9) by electric mill and were filtered simoltaneously. In the fourth method, new plants, in accordance with dry matter content of each organ, were grounded by electric mill and soaked for a week with the solvents of methanol and acetic acid (ratio 1:9) and then were filtered. Then, all samples were concentrated to 30 ml. A total of 32 samples were obtained and the composition of quercetin and kampferol was measured by high-performance liquid chromatography (HPLC). Results showed that most of the quercetin in Foeniculum vulgare Mill., obtained in flower (2990ppm), leaves (1223ppm) and seed (1779ppm) was related to the method of extraction with methanol, and in stem (1316ppm) was related to the method of maceration with methanol-acetic acid. Also, the lowest quercetin obtained in flower (17ppm), leaves (15ppm), stem (9ppm), and seed (50ppm) was related to the first method (extraction with chloroform). In the same culture conditions, the highest value of kampferol obtained in flower (912ppm), leave (273ppm), stem (184ppm) and seed (1142ppm), was related to the method of maceration with methanol-acetic acid while the lowest kampferol obtained in flower (209), leave (55), stem (45) and seed (427), was related to the chloroform extraction method.
M. Niknejad; M.H. Lebaschy; K. Jaimand; F. Hatami
Abstract
Manure management is a major factor in cultivation of medicinal plants. Utilization of proper fertilizers due to optimizing the plant growth could have positive effects on quality and quantity indicators. In order to investigate the effects of organic and chemical fertilizers on essential oil and components ...
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Manure management is a major factor in cultivation of medicinal plants. Utilization of proper fertilizers due to optimizing the plant growth could have positive effects on quality and quantity indicators. In order to investigate the effects of organic and chemical fertilizers on essential oil and components of Matricaria chamomilla L., an experiment was conducted using complete randomized blocks design with three replications at Research Institute of Forests and Rangelands during 2009-2010. Treatments were five fertilizers; chemical fertilizer (Ammonium phosphate) 150 Kg/ha, organic mixture (Bovine) 12 tons/ha, and chemical fertilizer 75 kg/ha, vermicompost (derived from wood waste) 7500 kg/ha, compost (plant debris) 24 tons/ha and control (no fertilizer). Results showed that the mixture treatment (organic and chemical) had the most influence on increasing the quality and quantity of phytochemical characteristics. Maximum essential oil yield (0.37 gr) and beta-ocimene (0.43%) were obtained with mixed fertilizer bysignificant difference compared to control and other treatments. Mixed fertilizers treatment caused an increase in most of the other compounds of Matricaria chamomilla compared with other treatments.
K. Jaimand; M.B. Rezaee; S.R. Tabaei Aghdaei; M. Nadery Hajibagher Kandy; S. Meshkizadeh
Abstract
In this study, tannin was extracted from rose water, wastewater and petal residue of Rosa damascena Mill. Twenty one accessions collected from different provinces of Iran and cultivated in the Research Institute of Forests and Rangelands were sampled for extraction. Then, each sample of essential oil, ...
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In this study, tannin was extracted from rose water, wastewater and petal residue of Rosa damascena Mill. Twenty one accessions collected from different provinces of Iran and cultivated in the Research Institute of Forests and Rangelands were sampled for extraction. Then, each sample of essential oil, rose water, wastewater and petal residue were prepared. The amount of tannin in the samples was measured by colorimetric method and spectrophotometer apparatus (Model 340 Hitachi) at 760 nm. The highest amount of tannin in rose water, wastewater and petal residue was respectively obtained from Khuzistan and Ilam (2163 ppm), Qom (1846 ppm), and Gillan (1432 ppm) accessions for rose water, Zanjan (1919 ppm), Mazandaran (1895 ppm) Qom, Gilan, Lorestan and Isfahan (1871 ppm) accessions for wastewater, and Yazd (3432 ppm), Sistan and Baluchestan (3139 ppm) and Kohgiluyeh-Boyer Ahmad (2993 ppm) accessions for petal residual. According to the results, it is recommended to extract tannin from samples used in rose water production. In other words, this will be economically useful for rose water traditional producers.
A. Akbarzadeh; K. Jaimand; A. Hemmati; B. Khanjani shiraz
Abstract
The present study was carried out to collect, identify and determine medical plants dispersion in the Gilan province during 2001-2006. This study was carried out to identify tree and shrub species and herbaceous plants in the Gilan. For thes purpose at first the floristic list of Gilan was prepared, ...
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The present study was carried out to collect, identify and determine medical plants dispersion in the Gilan province during 2001-2006. This study was carried out to identify tree and shrub species and herbaceous plants in the Gilan. For thes purpose at first the floristic list of Gilan was prepared, then lirerature review of the plants parameters were used for indemnification of the plants. In this study, 342 species, 229 genuses belonging to 95 familities were distinguished. These results showed 24 species belonged to Rosaceae, 23 species to Labiatae, 19 species to Compositae and the others belonged to other plant families.
K. Jaimand; M.B. Rezaee; M.H. Asareh; S.R. Tabaei Aghdaei; S. Meshkizadeh
Abstract
Flavonoid components are the largest phenolic groups in nature. Pigments are primarily responsible for the color of rose petals (the colorless flavonoids aid the intensification and stabilization of petals color). The aim of this research was extraction and determination of flavonoid compounds kaempferol ...
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Flavonoid components are the largest phenolic groups in nature. Pigments are primarily responsible for the color of rose petals (the colorless flavonoids aid the intensification and stabilization of petals color). The aim of this research was extraction and determination of flavonoid compounds kaempferol and quercetin in petals of Rosa damascena from western of Iran and cultivated in Research Institute of Forests and Rangelands. Samples were collected on late April 2006. A 5 g fresh flower petals was blended with 30 ml of 9:1 methanol:acetic acid, the extract was analyzed by high performance liquid chromatography (HPLC). Two flavonoids kaempferol and quercetin glycoside compounds were extracted from ten samples. The highest kaempferol content were obtained from Kordestan (595 ppm), Qazvine (505 ppm), West Azarbaijan (484 ppm), and Ilam (483 ppm), and the lowest from Kermanshah (74 ppm), Hamedan (88 ppm) and Lorestan (Poldokhtar) (252 ppm). The highest quercetin glucoside contents were obtained from Ardabil (322 ppm), West Azarbaijan (312 ppm), Ilam (302 ppm), and Chahar Mehal (239 ppm), and lowest were from Lorestan (Poldokhtar) (77 ppm), Hamedan (80 ppm), Kermanshah (117 ppm) and Kohkiluyeh (Gachsaran) (158 ppm). According to these results, the best samples were obtained from west Azarbaijan (484 ppm kaempferol, 312 ppm quercetin), Ilam (483 ppm kaempferol, 302 ppm quercetin) and Ardabil (447 ppm kaempferol, 322 ppm quercetin).
M. Habashi; M. Mirza; Y. Mostofi; K. Jaimand
Volume 24, Issue 4 , February 2009, , Pages 428-436
Abstract
The citron (Citrus medica L.) belongs to Rutaceae family. Citron peel oil is widely used in perfume industry, medicine, and food products. In this research the fruits of citron were collected from North of Iran in the fall and peel oil obtained by hydrodistillation and cold-press methods with the ...
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The citron (Citrus medica L.) belongs to Rutaceae family. Citron peel oil is widely used in perfume industry, medicine, and food products. In this research the fruits of citron were collected from North of Iran in the fall and peel oil obtained by hydrodistillation and cold-press methods with the extraction efficacy of 0.6% and 0.5% (w/w), respectively. The oils were analyzed by GC and GC-MS. Twenty-five components were identified in the hydrodistilled oil. The main components were limonene (58.3%), γ-terpinene (16.8%), geranial (6%) neral (4.8%), geranyl acetate (1.4%) and geraniol (1.36%). Twenty-three components were characterized in cold-pressed oil with limonene (63.7%), γ-terpinene (21.7%) and geranial (1.3%) as the main components.
M.B. Rezaee; K. Jaimand; V. Mozaffrian
Volume 24, Issue 3 , November 2008, , Pages 271-277
Abstract
The volatile constituents of Anthemis coelopoda Boiss. were isolated by hydrodistillation and analyzed by GC and GC/MS. In this study samples were collected from Gilan province in Rodbar on late May 2003. According to literature, this species was not the subject of research up to now and therefore its ...
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The volatile constituents of Anthemis coelopoda Boiss. were isolated by hydrodistillation and analyzed by GC and GC/MS. In this study samples were collected from Gilan province in Rodbar on late May 2003. According to literature, this species was not the subject of research up to now and therefore its chemical composition is not well known. The major constituents of A. coelopoda Boiss. flower oil were Cis-chrysanthenyl acetate (27.3%), hexyl butanoate (16%), and myrcene (7%), while the leaf oil contained isobornyl formate (30.6%), Trans-ethyl chrysanthemumate (15%) and p-mentha-1,5-diene-8-ol (13.7.4%).
K. Jaimand; M.B. Rezaee; A. Najafi Ashtiany
Volume 23, Issue 2 , August 2007, , Pages 262-268
Abstract
Crocus sativus L. is one of the most important plants in Khorasan provience and exported to the whole world. It is used in food for color and flavour and also in medicine. In this study effects of storage condition of Crocus sativus L. for determining crocin were under investigation in Khorasan ...
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Crocus sativus L. is one of the most important plants in Khorasan provience and exported to the whole world. It is used in food for color and flavour and also in medicine. In this study effects of storage condition of Crocus sativus L. for determining crocin were under investigation in Khorasan Provience. Samples were collected on November 2003. We kept samples for 20 months in three different conditions as light, darkness and refrigerator on zero degree. On November 2005 samples were analyzed by High Performance Liquid Chromatography (HPLC) which crocin percentage content in light were 0.032%, dark 0.038% and refrigerator 0.028%. According to the results the best condition to kept stigmata is darkness.
K. Jaimand; M.B. Rezaee; Z. Abravesh; M. Golypour; M. Sharifee
Abstract
Oleuropein, a compound that cause the bitter taste of olive, has many pharmacological properties. It is a natural antioxidant. In this study, the leaves of nine cultivars of Olea europaea L. named: Olive Begonia, Olive Gorgan, Olive Dezful, Olive Khshavy, Olive Khoramabady, Olive Dagal, Olive Barbar, ...
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Oleuropein, a compound that cause the bitter taste of olive, has many pharmacological properties. It is a natural antioxidant. In this study, the leaves of nine cultivars of Olea europaea L. named: Olive Begonia, Olive Gorgan, Olive Dezful, Olive Khshavy, Olive Khoramabady, Olive Dagal, Olive Barbar, Olive Zahedy and Olive Red were collected from Fadak station in Dezful city (Khozestan province, western south of Iran) at September 2004. After extraction of leaves by methanol, the oleuropein contents of extracts were determined by HPLC. Maximum amount of oleuropein was found in Olive Khoramabady (0.08 mg/ml, 0.24% w/w) and minimum in Olive Dagal (0.04 mg/ml, 0.13% w/w).
K. Jaimand; M.H. Assareh; M.B. Rezaee; M.M. Brazandeh
Abstract
Two Eucalyptus species , E. stricklandii Maiden. and E. erythrocorys F. Muell were cultivated on 1994 in south-western of Iram (Khozestan), were collected on April 2004. The essential oils isolated by hydrodistillation from the leaves of were analyzed by GC and GC/MS.The main components identified ...
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Two Eucalyptus species , E. stricklandii Maiden. and E. erythrocorys F. Muell were cultivated on 1994 in south-western of Iram (Khozestan), were collected on April 2004. The essential oils isolated by hydrodistillation from the leaves of were analyzed by GC and GC/MS.The main components identified in E. stricklandii oil were 1,8-cineole (72.2%), a-pinene (12.2%) and terpinen-1–ol (2.8%), and in E. erythrocorys were 1,8-cineole (80%), a-pinene (5.7%) and terpinen-1-ol (2.2%). High content of 1,8-cineole in our cultivated plants and uses of this compound in pharmacy industry is a reason of our investigation. This paper now reportes for the first time the results of the analyses of leaf oils of E. stricklandii Maiden.,and E. erythrocorys F. Muell. , growing in Iran.
K. Jaimand; M.B. Rezaee; M.H. Assareh; M.M. Brazandeh
Abstract
In this research, essential oils were extracted by hydro-distillation method but with different apparatus of distillation from Rosa damascena Mill. The oil obtained at different yields for example for Clevenger (0.008%), Miquel (0.014%), A.O.A.C. (0.012%) and two plans designed in Research Institute ...
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In this research, essential oils were extracted by hydro-distillation method but with different apparatus of distillation from Rosa damascena Mill. The oil obtained at different yields for example for Clevenger (0.008%), Miquel (0.014%), A.O.A.C. (0.012%) and two plans designed in Research Institute of Forests and Rangelands by authors which were named plan-1 (0.015%) and plan-2 (0.023%). Samples were analyzed by GC and GC/MS. The main constituents of the oil by Clevenger were geraniol (26.7%), n-nonadecane (21.3%), citronellol (12.5%) and n-henicosane (12.0%); by Miquel were geraniol (32.9%), citronellol (19.0%), n-nonadecane (10.6%); by A.O.A.C. were n-nonadecane (28.5%), geraniol (22.6%), n-henicosane (10.6%) and citronellol (10.2%) in plan -1 were n-nonadecane (21.3%), geraniol( 21.8%), citronellol (12%) and in plan-2 were n-nonadecane (21.8%), geraniol (19.1%) and citronellol (15%), respectively. By comparison of the results from different apparatus of hydro-distillation, it can be concluded that the best plan for obtaining essential oil from Rosa damascena Mill., were plan-2 and Miquel.
K. Jaimand; M.B. Rezaee; A.N. Ashtiany
Abstract
Phenolic compounds in olive fruits are important factors to consider. These compounds are partly responsible for auto-oxidation stability and organoleptic characteristics. Moreover, they have pharmacological properties and are natural antioxidants and inhibit gram- positive microorganisms involved in ...
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Phenolic compounds in olive fruits are important factors to consider. These compounds are partly responsible for auto-oxidation stability and organoleptic characteristics. Moreover, they have pharmacological properties and are natural antioxidants and inhibit gram- positive microorganisms involved in the fermentation of olive fruits. Oleuropein, the main component that produced bitterns in olive, is a heterosidic ester of elenolic acid and 3,4-dihydroxyphenylethanol. In this study we have collected two samples in summer 2004 from washing exude of fruits Olea europaea L., after procedure for the separation of phenolic compounds extracted. Identification and determination of oleuropein compound was done by HPLC. The results showed the quantity of oleuropin in the salt water sample was 0.0010% mg/ml and in NaOH sample was 0.0016% mg/ml.
S.R. Tabaei-Aghdaei; M.B. Rezaee; K. Jaimand
Abstract
Rosa damascenaMill. genotypes werecollected from central parts of Iran, and cultivated at the experimental field of Research Institute of Forests and Rangelands, using a randomized complete blocks design with three replications. Genotypes from six provinces were evaluated for essential oils yield and ...
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Rosa damascenaMill. genotypes werecollected from central parts of Iran, and cultivated at the experimental field of Research Institute of Forests and Rangelands, using a randomized complete blocks design with three replications. Genotypes from six provinces were evaluated for essential oils yield and number of floral parts. Genotypes showed differences in terms of the measured traits. Essential oils concentration and yield showed variation. The most essential oils percentage (0.05%) and yield were observed in Isfahan7 and Yazd1 accessions, respectively, in 2002. Yazd2 and Isfahan6 accessions showed the highest essential oils percentage (0.03%) in 2001, and the most percentage of oils was observed in accession collected from Tehran, in 2003. Also, accession of Yazd1 demonstrated the highest 3-year mean of essential oils percentage and yield. Furthermore, the accessions showed variation for floral parts number. The highest numbers of petals and pistils were observed in accession collected from Tehran Province. Also, Isfahan10 accession showed the highest number of stamens. A considerable variation was, therefore, revealed for floral parts and essential oil content of Rosa damascena genotypes from central parts of Iran. It could then be concluded that the Rosa damascena genotypes differ for essential oils content and this may be applied for classification of Damask rose genotypes. Finally yield of essential oils as the major product of Rosa damascena could be considered as an appropriate selection factor, in order to improve rose varieties.
K. Jaimand; P. Baghai; M.B. Rezaee; S.A. Sajadipoor; M. Nasrabadi
Volume 20, Issue 3 , October 2004, , Pages 323-331
Abstract
Juglone is a naphtoquinone compound present in walnut (Juglans regia L. ; Juglandaceae ) leaves and peels.that have been broadly used in traditional medicine for many years. In this research, samples were collected during June to November 2001 and (10 g) extract with a Soxhlet apparatus for 2 hours. ...
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Juglone is a naphtoquinone compound present in walnut (Juglans regia L. ; Juglandaceae ) leaves and peels.that have been broadly used in traditional medicine for many years. In this research, samples were collected during June to November 2001 and (10 g) extract with a Soxhlet apparatus for 2 hours. The concentrate of the acetone extract of the samples was defatted and then extracted with chloroform. The chloroform solution was evaporated under reduced pressure to give a dry residue recoverd by methanol. The extract was analysed by HPLC. Joglone content in leaves was from 0.021 mg/ml to 0.114 mg/ml and in peels were 0.035 mg/ml to 0.534 mg/ml , respectively.
K Jaimand; M.B. Rezaee
Volume 20, Issue 2 , July 2004, , Pages 181-190
Abstract
The compostion of the oils from flower and leaf of Achillea mellifolium L. subsp.mellifolium were collected during the flowering period from Lar area to Damavand montain, alt. 3400 m. in July 2003. The essential oils obtained by different methods of distillation ( steam distillation and hydrodistillation), ...
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The compostion of the oils from flower and leaf of Achillea mellifolium L. subsp.mellifolium were collected during the flowering period from Lar area to Damavand montain, alt. 3400 m. in July 2003. The essential oils obtained by different methods of distillation ( steam distillation and hydrodistillation), the percentage of oils for flower and leaf by steam distillation were 0.2 and 0.05% and by hydrodistillation were 0.4 and 0.04% respectively, and calculated on the dry weight, and analysis by GC and GC/MS. The major constituents by hydrodistillation in flower were p-cymene (19.8%), n- heptanol (15.2%) and bornyl acetate (12%) in leaf p- cymene (24.1%), n- heptanol (11.1%) and camphor (5.9%) and by steam distillation in flower were (E)-isoeugenyl acetate (18%), n- heptanol (16.3%), bornyl acetate (16.2%) and p-cymene (9.3%) in leaf (Z)- isodemicin (16%), (E)-isoeugenyl acetate (14.9%), nootkatin (13%) and longifolene (11.8%), respectively
