F. Hooshidari; F. Sefidkon; Z. Jamzad
Volume 22, Issue 3 , November 2006, , Pages 209-215
Abstract
The aerial parts of Salvia bracteata were collected in two stages of plant growth (before flowering and full flowering) from Chenareh-marivan (Kurdistan province) in west of Iran .The essential oils were obtained by hydro-distillation method. The oils were analyzed by GC and GC/MS. The yields of essential ...
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The aerial parts of Salvia bracteata were collected in two stages of plant growth (before flowering and full flowering) from Chenareh-marivan (Kurdistan province) in west of Iran .The essential oils were obtained by hydro-distillation method. The oils were analyzed by GC and GC/MS. The yields of essential oils were 0.22% before flowering stage and 0.28% full flowering stage. Before flowering stage 17 compounds and full flowering stage 19 compounds were characterized. In both of stages, β-caryophyllen and γ-muurolene devoted the highest rates of the compounds. β-caryophyllen had a decreasing trend from before flowing to flowering so that it changes from 49.59% to 41.64% vs. γ-muurolene so the rate of latter compound changed from 18.30% to 22.78%. In samples from Chenareh, Marivan, 17 constituents were common at two stages. (E)-β-ocimene was only before flowering stage and bornyl-acetate, δ-elemene, β-bourbonene were found only at full flowering stage.
B. Abbaszadeh; E. Sharifi Ashourabadi; M.R. Ardakani; M.H. Lebaschi; F. Safikhani; M. Naderi Hadjibagher Kandi; F. Sefidkon
Volume 22, Issue 3 , November 2006, , Pages 223-230
Abstract
In order to investigation of application methods of nitrogen fertilizer on essential oil content and composition of Balm, the experiment was conducted during 7 months in field condition at Alborze Research complex in 2004. This experiment was carried out at two levels of suspension nitrogen (4.5% and ...
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In order to investigation of application methods of nitrogen fertilizer on essential oil content and composition of Balm, the experiment was conducted during 7 months in field condition at Alborze Research complex in 2004. This experiment was carried out at two levels of suspension nitrogen (4.5% and 6%) spraying on shoot and two levels of solid nitrogen (60 and 90 kg N/ha) application in soil with control. Treatment was implicated to three equal divisions. This experiment was carried out in complete randomized block design with 4 replications. In floral imitation, plants harvested and essential oil were extracted by water distillation. The essential oils were analyzed by GC and GC/MS. The results showed a significant difference (1%) among nitrogen rates application on essential oil yield. Identification of essential oil components showed that some compounds of the oil decreased with more application of nitrogen in two methods of nitrogen application, whereas some other compounds increased with most application nitrogen in both methods.
A. Hassani
Volume 22, Issue 3 , November 2006, , Pages 256-261
Abstract
Moldavian balm (Dracocephalum moldavica) is an annual herb plant belonging to the Lamiaceae family. Moldavian balm is considered stomachic and sedative. Essential oil of this plant are used in pharmaceutical and food industries and possesses antimicrobial feature. To study the effects of different levels ...
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Moldavian balm (Dracocephalum moldavica) is an annual herb plant belonging to the Lamiaceae family. Moldavian balm is considered stomachic and sedative. Essential oil of this plant are used in pharmaceutical and food industries and possesses antimicrobial feature. To study the effects of different levels of water stress on growth, yield and essential oil content of Moldavian balm, a pot experiment in randomized complete block design with four treatments and four replications was conducted. Water stress treatments were: 100% of field capacity (non-stress), 85% of field capacity (mild water stress), 70% of field capacity (moderate water stress) and 55% of field capacity (severe water stress). According to the results of statistical analysis, water stress had significant effect on growth parameters, herb yield and essential oil yield. As the soil water content decreased, plant height, stem diameter, number and length of auxiliary shoots, fresh and dry herb yield in pot and essential oil yield decreased. There was no significant difference between water stress treatments for essential oil content. The highest amounts of essential oil content (0.35 ml/100gr dry weight) and essential oil yield (0.115 ml/pot) were observed in 70% and 100% of field capacity, respectively.
F. Askari; F. Sefidkon; Sh. Ahmadi
Volume 22, Issue 2 , September 2006, , Pages 98-104
Abstract
trans-a-Bergamotene is an aromatic compound that is used for synthesize of the other aromatic compounds. Pimpinella affinis Ledeb. grows wild in different regions of Iran, north, north-west, center, eastern and north-east. In This research, the aerial parts and seeds of P. affinis werecollected ...
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trans-a-Bergamotene is an aromatic compound that is used for synthesize of the other aromatic compounds. Pimpinella affinis Ledeb. grows wild in different regions of Iran, north, north-west, center, eastern and north-east. In This research, the aerial parts and seeds of P. affinis werecollected from Noshahr in 2003 and 2004. Essential oils were isolated by hydro-distillation. The yields of stem plus the leaf, inflorescence and seed oils for the first year were 0.26%, 1/1% and 4.1% w/w and for the second year were 0.26%, 0.86% and 2.45%, respectively. Four constituents in stem plus the leaf oil, five constituents in inflorescence oil and six constituents in seed oil were identified in samples of 2003. Six constituents in stem plus the leaf oil, fifteen constituents in inflorescence oil and six constituents in seed oil were identified in samples of 2004. Major constituent of the stem plus the leaf, inflorescence, and seed oils was trans-a-Bergamotene (91.1%, 96.2% and 90.2% in 2003 and 94.3% 84.9% and 95.5% in 2004, respectively).
R. Abbas Azimi; F. Sefidkon; Z. Jamzad; Gh. Bakhshi Khaniki
Abstract
Vitex L. (250 species) is a large tropical genus with a few temperate species. Examining the morphological characters of leave, stem, corolla and calyx together, with the micro-morphological observations, revealed the occurrence of four species in the flora of Iran. These species are V. agnus-castus, ...
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Vitex L. (250 species) is a large tropical genus with a few temperate species. Examining the morphological characters of leave, stem, corolla and calyx together, with the micro-morphological observations, revealed the occurrence of four species in the flora of Iran. These species are V. agnus-castus, V. negundo, V. trifolia and V. pseudo-negundo. In this research, four species of Vitex werecollected in flowering stage from their natural habitats. Hydro-distillation method was used to obtaining the essential oils. Essential oils components were identified by capillary GC and GC/MS. In the oil of V. agnus-castus, the main components of essential oil were monoterpenoids (82.8%) with 1,8-cineole (18.5%), α-pinene (17.8%) and limonene (15.7%) as major components. In the oil of V. pseudo-negundo, the main components of the essential oil were sesquiterpenoids (50.7%) which have the highest amount in comparing with other species and include α-guaiene (14.2%), germacrene D (11/6%) and α-cadinol (10%). Also the monoterpenoids of the oil of this species were α-pinene (10.3-24.5%), limonene (4.4-12.8%) and 1,8-cineole (10.9%). In the oil of V. negundo the major components were monoterpenoids (66.6%), among them 1,8-cineole (20.8%) and α-pinene (18.8%) were the main components. In the oil of V. trifolia, the major components were monoterpenoids (67.1%) and the main constituents were identified as sabinene (24.6%) and α- pinene (23.9%). 1,8-cineole was not found in the oil of this species. β-caryophyllene was identified at higher percentage (10.5%) in the oil of V. trifolia
M.H. Assareh; Z. Abravesh; M.B. Rezaee
Abstract
The leaves of Eucalyptus caesia were collected from north khuzistan in March 2003. The essential oil of Eucalyptus caesia (Myrthaceae) was prepared by hydro-distillation and analyzed by GC and GC/MS. The essential oil was produced at yield of 0.97% (based on dry weights). Twenty-one components were identified, ...
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The leaves of Eucalyptus caesia were collected from north khuzistan in March 2003. The essential oil of Eucalyptus caesia (Myrthaceae) was prepared by hydro-distillation and analyzed by GC and GC/MS. The essential oil was produced at yield of 0.97% (based on dry weights). Twenty-one components were identified, among them α-pinene (9.3%), 1,8-cineole (69.4%), trans-pinocarveole (2.4%), caryophyllene (6.1%) and globulol (2.8%) were the major constituents.
K. Jaimand; M.H. Assareh; M.B. Rezaee; M.M. Brazandeh
Abstract
Two Eucalyptus species , E. stricklandii Maiden. and E. erythrocorys F. Muell were cultivated on 1994 in south-western of Iram (Khozestan), were collected on April 2004. The essential oils isolated by hydrodistillation from the leaves of were analyzed by GC and GC/MS.The main components identified ...
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Two Eucalyptus species , E. stricklandii Maiden. and E. erythrocorys F. Muell were cultivated on 1994 in south-western of Iram (Khozestan), were collected on April 2004. The essential oils isolated by hydrodistillation from the leaves of were analyzed by GC and GC/MS.The main components identified in E. stricklandii oil were 1,8-cineole (72.2%), a-pinene (12.2%) and terpinen-1–ol (2.8%), and in E. erythrocorys were 1,8-cineole (80%), a-pinene (5.7%) and terpinen-1-ol (2.2%). High content of 1,8-cineole in our cultivated plants and uses of this compound in pharmacy industry is a reason of our investigation. This paper now reportes for the first time the results of the analyses of leaf oils of E. stricklandii Maiden.,and E. erythrocorys F. Muell. , growing in Iran.
E. Alishahi-Noorani; F. Sefidkon; M. Yoosefzadi; S. Neamati; M. Khajeh-piri
Abstract
The Genus Salvia from Labiatae family consists of 700 species in the world, 58 species in Iran. The plant materials of S. chloroleuca were collected from 18 km on Chalus. The Genus Nepeta from Labiatae family has vast distribution in all over of Iran. The aerial parts of N. fissa were collected from ...
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The Genus Salvia from Labiatae family consists of 700 species in the world, 58 species in Iran. The plant materials of S. chloroleuca were collected from 18 km on Chalus. The Genus Nepeta from Labiatae family has vast distribution in all over of Iran. The aerial parts of N. fissa were collected from 4km on Ab-Ali. In this research, at first the essential oils were extracted by hydro-distillation from the aerial parts of S. chloroleuca and N. fissa. The oils were analyzed by capillary GC and GC/MS. Twelve compounds were identified in the oil of S. chloroleuca with Germacrene D (37.4%) β-cayophyllene (37%) and bicyclogermacrene (7.1%) as Main constituent. Forty-two components were characterized in the oil of N. fissa with Caryophyllene oxide (12.3%) Valencene (6.6%) and β- pinene(6%) as main compound. Then the antimicrobial activities of the essential oils were evaluated by disk diffusion method using: Bacillus subtilis (G+), Staphylococus aureus (G+) ATCC 25923, Pseudomonas aeruginosa (G-) ATCC 85327, Echerchia coli (G-) ATCC 25992. The results indicated that the oil of S. chloroleuca had the greatest antibacterial effect against mentioned bacteria, on E. coli and S. aureus were mid- resistant.
R. Omidbaigi; K. Sadrai Menjili; F. Sefidkon
Abstract
To study the effect of sowing date on fennel CV. Soroksari, this research work was carried out on randomized complete block design with three replications. The effects of sowing dates of 6 of March, 20 of March, 4 of April, 19 of April, 5 of May, 21 of May, 5 of June and 21 of June, were studied on growth, ...
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To study the effect of sowing date on fennel CV. Soroksari, this research work was carried out on randomized complete block design with three replications. The effects of sowing dates of 6 of March, 20 of March, 4 of April, 19 of April, 5 of May, 21 of May, 5 of June and 21 of June, were studied on growth, development, seed yield and essential oil and anethole content of fennel CV. Soroksari. The results show that sowing dates hade significant effect on growth, development and quality and quantity of active substances of fennel. On the basis of the results the seeds were sown on 21 of May the seeds were germinated and developed faster. The highest plant (150.8 cm) and the largest number of branches per plant (10.1) was obtained from the plants were sown on 4 of April. There was no significant effect among 1000 seeds weight and essential oil content of different sowing dates. The largest seed yield (1864.3Kg/ha) and essential oil yield (91.3Kg/ha) was obtained from the seeds were sown on 4 of April. Anethole content were decreased from the plants were sown from 6 of March to 21 of June, the lowest amount (58.2%) was extracted from the plants were sown on 21 of June. According to the results of this resesrch trial the most suitable date for sowing of fennel cv. Soroksari is recommended 20 of March to 4 of April.
Kh. Abbasi; F. Sefidkon; Y. Yamini
Abstract
The genus Satureja from lamiaceae family consists of many species all over the world. In Iran, there are 15 species, 9 of them are endemic. The oil of different Satureja species are very important because of having carvacrol. In this study, the effect of two extraction methods (hydro-distillation and ...
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The genus Satureja from lamiaceae family consists of many species all over the world. In Iran, there are 15 species, 9 of them are endemic. The oil of different Satureja species are very important because of having carvacrol. In this study, the effect of two extraction methods (hydro-distillation and supercritical fluid extraction) on yield and composition of the essential oils Satureja hortensis and Satureja rechingeri wasinvestigated. The aerial parts of S. hortensis were collected from Research Station of Alborz (Karaj) at full flowering stage and those of S. rechingeri were collected from their natural habitats on Ilam province. After drying the plant material, the oil of both species were obtained by hydro-distillation and also by SFE (with and without modifier). The oil were analyzed by GC and GC/MS. 17 compounds were identified in the oil of S. hortensis by hydro-distillation, with carvacrol (48.1%) and g-Terpinene (38.4%) as the main components. In the oils of SFE, 9 compounds were characterized with carvacrol (57.1%-63.4%) and g-Terpinene (27.0%-32.8%) as the major components. Therefore, the percentage of carvacrol was increased by SFE and g-Terpinene was decreased. 20 components were identified in the oil of S. rechingeri by hydro-distillation, with carvacrol (86.6%) as main constituent. In the oils of SFE, 6 compounds were identified, that the percentage of carvacrol (95.0%-95.6%) was higher.
Y. Imani
Abstract
In order to investigate the quantitative changes of essential oil during growth period (vegetative, flowering, after flowering) in Melissa officinalis, samples were collected from Arasbaran and Malekan for two years (2001 and 2002). All samples were dried in laboratory condition and then essential oils ...
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In order to investigate the quantitative changes of essential oil during growth period (vegetative, flowering, after flowering) in Melissa officinalis, samples were collected from Arasbaran and Malekan for two years (2001 and 2002). All samples were dried in laboratory condition and then essential oils were extracted by steam distillation method. The oils were dried over anhydrous sodium sulfate and weighted. Combined analysis of data was done with C.R.D design in 3 replications. Combined analysis of data for Melissa officinalis indicated that effects of year, location and phenology phases weren’t significantly different, but the interaction of (year × location) and (location × year × phenology phases) were significantly different at 1% probability level. Means were grouped using L.S.D (5%). Based on the results of this study, essential oil yield in Malekan was higher at first year at flowering stage (0.197%). Mean of interaction (year × location) showed that oil yield of Melissa officinalis in Malekan at first and second year were respectively (0.108 and 0.080%), and in Arasbaran were (0.035 and 0.018%). The results of the two years showed that the average oil content at first year was more than that at second year.
S.R. Tabaei-Aghdaei; M.B. Rezaee; K. Jaimand
Abstract
Rosa damascenaMill. genotypes werecollected from central parts of Iran, and cultivated at the experimental field of Research Institute of Forests and Rangelands, using a randomized complete blocks design with three replications. Genotypes from six provinces were evaluated for essential oils yield and ...
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Rosa damascenaMill. genotypes werecollected from central parts of Iran, and cultivated at the experimental field of Research Institute of Forests and Rangelands, using a randomized complete blocks design with three replications. Genotypes from six provinces were evaluated for essential oils yield and number of floral parts. Genotypes showed differences in terms of the measured traits. Essential oils concentration and yield showed variation. The most essential oils percentage (0.05%) and yield were observed in Isfahan7 and Yazd1 accessions, respectively, in 2002. Yazd2 and Isfahan6 accessions showed the highest essential oils percentage (0.03%) in 2001, and the most percentage of oils was observed in accession collected from Tehran, in 2003. Also, accession of Yazd1 demonstrated the highest 3-year mean of essential oils percentage and yield. Furthermore, the accessions showed variation for floral parts number. The highest numbers of petals and pistils were observed in accession collected from Tehran Province. Also, Isfahan10 accession showed the highest number of stamens. A considerable variation was, therefore, revealed for floral parts and essential oil content of Rosa damascena genotypes from central parts of Iran. It could then be concluded that the Rosa damascena genotypes differ for essential oils content and this may be applied for classification of Damask rose genotypes. Finally yield of essential oils as the major product of Rosa damascena could be considered as an appropriate selection factor, in order to improve rose varieties.
M.M. Barazandeh
Abstract
In order to increas the yield and quality of an eucalyptus essential oil(eucalyptus globulus) the leaves of plant was collected from Research Station of Zaghmarz in Pasansd of Behshahr and was identified by the staff of botanical department of Research Institute of Forests and Rangelands.Dried and ground ...
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In order to increas the yield and quality of an eucalyptus essential oil(eucalyptus globulus) the leaves of plant was collected from Research Station of Zaghmarz in Pasansd of Behshahr and was identified by the staff of botanical department of Research Institute of Forests and Rangelands.Dried and ground leaves of plant were steam and hydro distilled and fractions were collected every fifteen minutes. Proceeding of distillation time resulted to increas the yield and reduce cineole content. In steam distillation method,the oil yield was increased from 1.6% after 5 min. to 2.42% after 180 min. whereas 1,8-cineole content was reduced from 82.2% to 74.8%, respectively. In hydrodistillation method, the oil yield was increased from 1.55% after 15 min. to 2.86% after 180 min. whereas cineole content was reduced from 92.7% to 85.6%, respectively. Fifteen compounds were identified among which 1,8-cineole (74.8%-82.2%), α-pinene (6.28-7.70%) and limonene (5.70-6.20%) in steam distillation method and 1,8-cineole (85.60-92.70%), α-pinene (2.02%-2.37%) and limonene (2.04%-3.10%) in hydrodistillation method were the major constituents, respectively.
F. Sefidkon
Volume 20, Issue 2 , July 2004, , Pages 149-158
Abstract
The genius of Echinophora presents 4 aromatic species in Iran, two of them are endemic. In this research, the aerial parts of E. sibthorpiana were collected in full flowering stage, from Tehran Provinc (north of Tehran). The plant materials were dried in room temprature and the essential oil isolated ...
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The genius of Echinophora presents 4 aromatic species in Iran, two of them are endemic. In this research, the aerial parts of E. sibthorpiana were collected in full flowering stage, from Tehran Provinc (north of Tehran). The plant materials were dried in room temprature and the essential oil isolated by steam distillation. The oil was analyzed by capillary GC and GC/MS. Among 17 compounds identified, the major components were delta-3-carene (31.8%), alpha-phellandrene (31.0%), methyl eugenol (16.9%), beta-phellandrene (5.3%) and p-cymene (4.7%). This oil could be used in cosmetic-hygenic industries because of the high amounts of delta-3-carene and alpha-phellandrene. These compounds have very good smells and used in perfumes.
Sh. Mehrpur; F. Sefidkon; H. Mirzaie-Nodoushan; A. Majd
Volume 20, Issue 2 , July 2004, , Pages 159-169
Abstract
To investigate quality and quantity of essential oil of Thymus kotschyanus in greenhouse and field cultivation, seeds of four populations of this species from Azarbayejan, Sirachal, Mianeh and Tabriz were collected and cultivated in greenhouse and field condititions. Kelevenger method was ...
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To investigate quality and quantity of essential oil of Thymus kotschyanus in greenhouse and field cultivation, seeds of four populations of this species from Azarbayejan, Sirachal, Mianeh and Tabriz were collected and cultivated in greenhouse and field condititions. Kelevenger method was used to extract the essential oils from the plants foliage. Essential oil components were analysed and recognised by GC and GC/MS. The components included Thymol, Carvacrol and r-cymene.The highest main percentage of Thymol(65.94%), and r-cymene(20.40%), were found in Azarbayejan population and Carvacrol (53.14%), in Sirachal population. The oil yeilds of Thymus kotschyanus in field were higher than greenhouse conditions, but Thymol and Carvacrol were decreased and other monoterpenes were the major components of the essential oils in greenhouse conditions. In field conditions, the percentage of r-cymene was high, comparing to greenhouse condition, but g- terpinene was trace.This problem can be related to temprature and other conditions in field and greenhouse conditions. On the other hand, these differences between the oil composition of the greenhouse, and field grown populations, may reveal a common route of formation the components in the species.
M. Mirza; M. Najafpour Navaei; M. Dini
Volume 20, Issue 2 , July 2004, , Pages 191-197
Abstract
The genus of Bothriochloa is a member of Gramineae , distributed in south of Europe, mediteranean region and south of Asia. In Iran one species is present in northern, northwest and western districts. The aerial parts of Bothriochloa ischaemum were collected from Karaj in Tehran province ...
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The genus of Bothriochloa is a member of Gramineae , distributed in south of Europe, mediteranean region and south of Asia. In Iran one species is present in northern, northwest and western districts. The aerial parts of Bothriochloa ischaemum were collected from Karaj in Tehran province at full flowering stage in july 2003. Essential oil isolated by hydrodistillation from the aerial parts of plant , were analysed by a combination of capillary GC and GC/MS. The yeild of essential oil was obtained 0.1% (w/w). Fourteen compounds in the oil have been identified representing 98% of the oil . The main constituents was viridiflorol (73%). Other main components were kessane, valencene and a-selinene.
M. Mirza; Z. Baher Nik; Z. Jamzad
Volume 19, Issue 2 , August 2003, , Pages 117-124
Abstract
The genus of salvia represents 58 species in Iran, 17 of which are endemic. One of the more distributed species is Salvia MirzayaniiRech. f. & Esfand. Essential oils isolated by hydro-distillation from the aerial parts of Salvia Mirzayanii, in flowering stage (April-2002) from Khash, were analyzed ...
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The genus of salvia represents 58 species in Iran, 17 of which are endemic. One of the more distributed species is Salvia MirzayaniiRech. f. & Esfand. Essential oils isolated by hydro-distillation from the aerial parts of Salvia Mirzayanii, in flowering stage (April-2002) from Khash, were analyzed by GC/MS. Twenty-eight components were identified that approximately constituent more than 99.1 % of the oil. The main constituents of the essential oil werelinalool 19%, linalyl acetate (12.9%), 1,8-cineol (12.1%), terpinenyl acetate (11.5%) as the major constituents respectively.
M. Niakan; R. Khavarynejad; M.B. Rezaee
Volume 19, Issue 1 , May 2003, , Pages 1-14
Abstract
The effects of two levels of nitrogen fertilizer (urea) (0,100 kg ha-1) with one of levels in phosphorus (super phosphate) and potassium (potass oxide ) fertilizer (each of 100 kg ha-1) in two rate of 0/100/100 kg ha-1 (N0 p100 k100، control) and 100/100/100 kg ha-1 (N100 P100K100) on quantity and quailty ...
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The effects of two levels of nitrogen fertilizer (urea) (0,100 kg ha-1) with one of levels in phosphorus (super phosphate) and potassium (potass oxide ) fertilizer (each of 100 kg ha-1) in two rate of 0/100/100 kg ha-1 (N0 p100 k100، control) and 100/100/100 kg ha-1 (N100 P100K100) on quantity and quailty of leaf (in before flowering) and flowering branches (in flowering) oil in Mentha piperita L. under the fram condition in form spilit plot design in four repeat have been evaluated. According to the results obtained, with out regard to kind of treatment, main composition in oil included: B-ocimene, linalool, 1,8- cineole, B caryophyllene, myrcene. In rate N0 P100 K100 (control), amount of linalool and 1,8 cineole in flowering branches were more of leaf oil. In before flower enhancement of nitrogen fertilizer increased amount of linalool and 1,8 cineol and reduced amount of B-caryophyllene and B-ocimene in leaf oil. In flowering branches, applied of nitrogen fertilizer increased amount of B-ocimene and B-caryophyllene and reduced linalool and 1,8 cineole. Amount of oil in flowering branches in control was more of leaf. Increased of nitrogen fertilizer, had positive effect on content leaf and flowering branches oil.
M.A. Soltani poor; A. Moradshahi; M.B. Rezaei; H. Mirzaei Nodoushan
Volume 19, Issue 1 , May 2003, , Pages 47-62
Abstract
Effects of various concentrations of essential oils (0, 2.5, 5, 10, 20, 50 and 100 percent) of Zhumeria majdae leaves collected from Sarchahan mountain on mitotic cell division and its stages in root cells of Allium cepa were studied in this investigation. Results showed that all of essential oil ...
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Effects of various concentrations of essential oils (0, 2.5, 5, 10, 20, 50 and 100 percent) of Zhumeria majdae leaves collected from Sarchahan mountain on mitotic cell division and its stages in root cells of Allium cepa were studied in this investigation. Results showed that all of essential oil concentrations reduced the number of mitotic cells. The average of mitotic cells in control was 7.5 % and in 100% concentration was zero, and also prophase, metaphase and anaphase phases were reduced. The most of decreament was in prophase stage. There are many terpenes compounds in essential oils of Zhumeria majdae leaves.They are probably a major effective factor in decrease of mitotic cell division in Allium cepa roots.
F. Sefidkon; S. Meshkizadeh; S. Shahrzad
Volume 18, Issue 1 , February 2003, , Pages 23-42
Abstract
Sequoia is a very big and ever green tree with 45-90 meter height. It is native to the side of Atlantic Ocean, Which is also cultivated in Iran. The plant materials were collected from one of the Sequoia trees from Rezvanshahr Garden in August. The height to this tree was 40 meter and it was 30 years ...
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Sequoia is a very big and ever green tree with 45-90 meter height. It is native to the side of Atlantic Ocean, Which is also cultivated in Iran. The plant materials were collected from one of the Sequoia trees from Rezvanshahr Garden in August. The height to this tree was 40 meter and it was 30 years old. For preparation of tissue culture samples, the end part of shoots in 1-2 centimeter length have been cultivated. The leaves from main tree and also tissue culture sample were hydro- distilled for their essential oils. The oils were analyzed by combination of capillary GC and GC/MS. 72 Compounds were identified in the leaf oil of main tree and 37 compounds were characterized in the leaf oil of tissue culture sample. The main components of the oil from main tree were β- phellandrene and limonene (13.30%), α- pinene (6.83%), terpinene –4-ol (6.47%), g-teroubebe *5.44%) and germacrene B (4.17%), while the major components of the oil from tissue culture sample were α- pinene (26.30%), α- terpinenyl acetate (14.40%), sabinene (13.60%), g- terpinene (7.10%), germacrene D (6.90%), b-phellandrene and limonene (6.60 %) and p-cymene (5.00%). Althoug these components were found in both oils, but the higher percentage of some useful compounds in the oil of tissue culture sample, gave it more effective character.
P. Salehi Shanjani; M. Mirza
Volume 17, Issue 1 , February 2003, , Pages 119-196
Abstract
Juniper is the most important native conifer in Iran. Juniper as one of the most resistant tree species to extreme conditions, frost (up to –40°C) and drought, plays important role in mountainous regions of Iran. In this study, by investigation on qualitative and quantitative characteristics ...
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Juniper is the most important native conifer in Iran. Juniper as one of the most resistant tree species to extreme conditions, frost (up to –40°C) and drought, plays important role in mountainous regions of Iran. In this study, by investigation on qualitative and quantitative characteristics of essential oils of leaves and berries (female cones), the effects of environmental factors were studied on metabolism of secondary products. The volatitale constituents of the Leaves and berries of male, female and male-female trees isolated by steam-distillation were analyzed by GC-MS.
Results indicated that the amount of essential oil in berries is higher than that in leaves. Seasonal alteration of essential oil of berries differs from leaves. The essential oil of berries increase during growth period, however decreased in leaves. Besides drying has increasing effect on essential oil.
In total 32 terpenoidic components were found that α-pinene, limonene, trans-verbenol, cis-verbenol, verbenone, Υ- elemone and elemol were the important ones. α-pinene up to 70% of essential oil content was the highest component. Seasonal alteration of each component was very variable. Drying effect can be valuable for isolation of components and commercial applications.
M. Najafpour Navaei; M. Mirza
Volume 18, Issue 1 , February 2003, , Pages 43-51
Abstract
The essential oils of Hyssopus officinalis from field and wild growing were studied by GC and GC/MS. The plant leaves were collected in the blooming stage on the experimental field in alborz karaj and nature in Roudbarak at Mazandaran province.Then they dried up and the essential oil isolated by ...
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The essential oils of Hyssopus officinalis from field and wild growing were studied by GC and GC/MS. The plant leaves were collected in the blooming stage on the experimental field in alborz karaj and nature in Roudbarak at Mazandaran province.Then they dried up and the essential oil isolated by steam distillation. Rt and KI were compared to the standard compounds then obtained components were recognized and their quality were defined per percent. Among 30 identified compounds in cultivated specimen the major components were iso-pinocamphone (32.4% ), pinocamphone (13.1%), β-pinene (12.2%) and pinocarvone(7.5%). In wild plant from 16 compounds iso-pinocamphone (49.4%), pinocamphone(18.7%), β-pinene (8.94%) and myrcene(5.8) were the main components. The oil obtained in yield of 0.6 % w/w in cultivated plant and 0.7 % in wild plant.
M.B. Rezaee; K. Jaimand; M. Moalemy
Volume 18, Issue 1 , February 2003, , Pages 123-135
Abstract
The volatile constituents from roots rhizoms of valeriana sisymbriifolia Vahl were collected from three different localities in Iran and the essential oils obtained by hydrodistillation method, the three samples yielded were Hamedan sample (0.08%), Este Azarbaijan sample (0.31%) and Esfehan sample (0.26%) ...
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The volatile constituents from roots rhizoms of valeriana sisymbriifolia Vahl were collected from three different localities in Iran and the essential oils obtained by hydrodistillation method, the three samples yielded were Hamedan sample (0.08%), Este Azarbaijan sample (0.31%) and Esfehan sample (0.26%) (V/W) essential oil on a dry weight basis, and analyzed by GC and GC/MS. The main components on Hamedan sample were a-pinene (14%), borneol (11.5%) and bornyl acetate (6.4%), Azarbaijan sample were a-pinene (16.6%), borneol (9.7%), b - himachalene(7.3%) and spathulenol (5%) and Esfehan sample were bornyl (13.53%), g - murrolene (5.2%) and humulene epoxide II (3.5%).
F. Sefidkon; A. Rahimi-Bidgoly
Volume 15, Issue 1 , January 2003, , Pages 1-22
Abstract
The aerial parts of Thymus kotschyanus Boiss & Hohen were collected in three stages of plant growth (before, at the beginning and complete flowering) from research station of Sirachal. The essential oils of plant materials were isolated by steam-, hydro- and water-steam distillation from the aerial ...
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The aerial parts of Thymus kotschyanus Boiss & Hohen were collected in three stages of plant growth (before, at the beginning and complete flowering) from research station of Sirachal. The essential oils of plant materials were isolated by steam-, hydro- and water-steam distillation from the aerial parts. The oils were analyzed by capillary GC and GC-MS. So, the effect of distillation methods and stages of plant growth on oil content and composition of Thymus kotschyanus was investigated. The oil yield, related to distillation method and stage of plant growth, was 0.28-1.80% w/w (the highest for complete flowering stage by hydrodistillation method). The main constituents in all of the oils were carvacrol (46.74-61.23%), thymol (7.51- 26.92 %), g-terpinene, p-cymene and borneol.
M. Mirza; Z. Baher Nik
Volume 14, Issue 1 , January 2003, , Pages 25-37
Abstract
Tilia platyphyllos which belongs to Tiliaceae family has aromatic flowers. The flowers were collected from Research Institute of Forests and Rangelands and extracted by hexane solvent. The absolute of linden was prepared by using Ethyl alcohol. The absolute were analyzed by GC/MS. Nineteen compounds ...
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Tilia platyphyllos which belongs to Tiliaceae family has aromatic flowers. The flowers were collected from Research Institute of Forests and Rangelands and extracted by hexane solvent. The absolute of linden was prepared by using Ethyl alcohol. The absolute were analyzed by GC/MS. Nineteen compounds reperesenting 96% of the absolute were identified of which Dihydrocarvone (29%), Limonene (12.9%), Caryophtllene (26%) and Carvone (6%) were the major constituents.
