In collaboration with Scientific Association of Iranian Medicinal Plants

Document Type : Research Paper

Authors

Research Institute of Forests and Rangelands

Abstract

Juniper is the most important native conifer in Iran. Juniper as one of the most resistant tree species to extreme conditions, frost (up to –40°C) and drought, plays important role in mountainous regions of Iran. In this study, by investigation on qualitative and quantitative characteristics of essential oils of leaves and berries (female cones), the effects of environmental factors were studied on metabolism of secondary products. The volatitale constituents of the Leaves and berries of male, female and male-female trees isolated by steam-distillation were analyzed by GC-MS.
Results indicated that the amount of essential oil in berries is higher than that in leaves. Seasonal alteration of essential oil of berries differs from leaves. The essential oil of berries increase during growth period, however decreased in leaves. Besides drying has increasing effect on essential oil.
In total 32 terpenoidic components were  found that α-pinene, limonene, trans-verbenol, cis-verbenol, verbenone, Υ- elemone and elemol were the important ones. α-pinene up to 70% of essential oil content was the highest component. Seasonal alteration of each component was very variable. Drying effect can be valuable for isolation of components and commercial applications.

اکبرزاده، م. 1373. تهیه نقشه پوشش گیاهی منطقه سیراچال به روش فلورستیک و فیزیونومیک. نشریه مؤسسه تحقیقات جنگلها و مراتع، شماره 92.
حجازی، 1357. اصول تشریح چوب.انتشارات دهخدا،‌صفحه 138 تا 140.
صادقی، ح. 1374. بررسی کمی و کیفی ترکیبهای رزین در کشت بافت و گیاه کامل کاج پایان‌نامه کارشناسی ارشد، دانشگاه تهران، دانشکده علوم.
صالحی شانجانی، پ. 1375. کشت بافت و اثر عوامل محیطی بر متابولیسم فرآورده‌های ثانوی و تغییرات پراکسیدازها و پلی فنل اکسیدازها در ارس. پایان‌نامه کارشناسی ارشد، دانشگاه تهران، دانشکده علوم.
صدری، م.ع. 1371. اسانس‌ها، مجله پژوهش و سازندگی، شماره 16، صفحه 10 تا 15
نیلوفری، پ. 1364. چوب‌شناسی چوب‌های ایران. انتشارات دهخدا، صفحه 143-144.
Adams, R. P., Thappa, R. K., Agarwal, S. q., Kapahi, B.K. and Sarin, Y.K., 1992. The wolatile leaf oils of Juniperus semiglobos from India Compared with J. exclesa M. Bieb. From Greece. J. Essent. Oil Res., 4: 214-219.
Adams, R.P., Cie-Lin, C.H. and Zhao-Zhen, Z., 1995. Comarisons of the volatile leaf oils of Juniperus rigida Mig. From northeastern  China, Korea and Japan. J, Essent. Oil Res., 7:49-52.
Adams, R. P., Gie-Lin, C.H. and Zhao-Zhen, Z., 1994a. The volatile leaf oils of Juniperus przewalskii Kom. and forma pendula. J. essent. Oil Res., 6: 17-20.
Adams, R. P., Cie-Lin, C.H. and Sho-Zhen, Z., 1994b. Comparison of the volatile leaf oils of Juniperus chinesis L., J. chinesis var. Kaizuca Hort. and J. pyramidalis from China. J. Essent oil Res. 6: 149-154.
Adams, R.P., Shatar, S. and Dembitsky, A.D. 1994c.
Comparison of the volatile leaf oils of Juniperus davurica Pall. From Mongolia with plants cultivated in Kazakhstan, Russia and scotland. J. Essent. Oil Res., 6:217-221.
Bats, J.P., Moulines, J.J., Boourgeois, G., Vacchiano, C., Coutiere, D. and Arnaudo, J.F., 1989. Chemical. Compositon of an industrial essential oil from Juniper branches. Congress of Esse tial oils, Fragrances and flavours, New Degkum India, 12-16.
Chatzopoulu, P.S. and Katsiotis, S.T. 1993. Study of the Essential oil from Juniperus commonis berries (cones) growing wile in Greece. Planta Med., 59:554-6.
Fournier, G and Gallen, G., 1991. Contribution to the study of the essential oil (of leaves) of various cultivars of Juniperus sabina. Planta Medica, 57(4):392-393.
Gara, R.I., Littke, W.R. and Rhoades, D.F. 1993. Emission of ethanol and monoterenos by fungal infected lodgepole pine trees. Phytochemistry, 34(4):987-990.
Kharebava. L.G. and Chavchanidze, V. Y., 1991. Comparision of essential oils of Juniperus leaves during different times of year. Subtropicheskie. Kul’tury, 3: 114-135.
Looman, A. and Svendsen, A.B., 1992. The needle essential oil of Norwegian mountain juniper, Juniperus commonis L. var. Saxalitis Pall. Flavour and Fragrance J., 7(11):23-26.
Macbonald, R.C. and Fall, R., 1993. Aceton emission from conifer buds. Phytochemistry, 34(4): 997-996.
Pradeep-Mishra, S., Agrawal, P.K. and Mishra, P., 1989. Some observation on the pharmacological activities of the essential oil Juniperus macropoda (J. excelsa). 60(4): 339-345.
Quinn, C.J. and Gadek, P.A., 1988. Sequence of xylen differentiation in leaves of cupressaceae. Americann Journal of Botany., 75(9): 1344-1351.
Rhoades, D.F., 1990. Analysis of monoterpens emitted and absorbed by undamaged boles of lodgepole pine. Phytochemistry., 24(5):1463-1465.
Sadri, H.A. and Assadi, M., 1994. Preliminary stadies on monoterpene composition of Juniperus polycarpos. IRAN J. Bot., 6(2).
Sezik, E. and Erosoz, T., 1986. Monoterpene hydrocarbones of essential oil of Juniperus foetidissima. Fitoterapia, 57(6): 442-444.
Vidrich, V., Michelozzi, M. and Fusi, P., 1991. Essential oil composition of leaves of Juniperus communis. Italia
Forestale e Montana, 46(4): 318-322.
Vidrich, V., Michelozzi, M., Bosetto, M. and Fusi, P., 1998. Initial study on the determination of Essential oils and tree sugars from commercial samples of Juniperus communis L. Subsp. Commonis fruit from central Italy. Monti e Boschi, 39(4): 57-60.
Vidrich, V., Cecconi, C., Bagnol, V. and Fusi, P. 1986. Seasonal variation in the composition of the essential oil of fruits of Juniperus excelsa. Italia forestal e Montana, 41(4): 184-196.
Yamanaka, K., 1984. Normal and traumatic resin-canls in the secondary phloem of conifers. J. of the Japon wood Research society, 30(5): 347-353.