Document Type : Research Paper

Authors

• E. Alishahi-Noorani ¹
• F. Sefidkon ²
• M. Yoosefzadi ³
• S. Neamati ³
• M. Khajeh-piri ³

¹ Academic member of Shahid Beheshti University

² Academic member f Research Institute of Forests and Rangelands

³ MS Students of Shahid Beheshti University

Abstract

The Genus Salvia from Labiatae family consists of 700 species in the world, 58 species in Iran. The plant materials of S. chloroleuca were collected from 18 km on Chalus. The Genus Nepeta from Labiatae family has vast distribution in all over of Iran. The aerial parts of N. fissa were collected from 4km on Ab-Ali. In this research, at first the essential oils were extracted by hydro-distillation from the aerial parts of S. chloroleuca and N. fissa. The oils were analyzed by capillary GC and GC/MS. Twelve compounds were identified in the oil of S. chloroleuca with Germacrene D (37.4%) β-cayophyllene (37%) and bicyclogermacrene (7.1%) as Main constituent. Forty-two components were characterized in the oil of N. fissa with Caryophyllene oxide (12.3%) Valencene (6.6%) and β- pinene(6%) as main compound. Then the antimicrobial activities of the essential oils were evaluated by disk diffusion method using: Bacillus subtilis (G+), Staphylococus aureus (G+) ATCC 25923, Pseudomonas aeruginosa (G-) ATCC 85327, Echerchia coli (G-) ATCC 25992.
The results indicated that the oil of S. chloroleuca had the greatest antibacterial effect against mentioned bacteria, on E. coli and S. aureus were mid- resistant.

Keywords

• Salvia chloroleuca
• Nepeta fissa
• antimicrobial effect
• essential oil