In collaboration with Scientific Association of Iranian Medicinal Plants

Document Type : Research Paper

Authors

1 MS student of Payam-e-Noor University

2 Academic member of Research Institute of Forests and Rangelands

3 Academic member of Tarbiat Modares University

Abstract

The genus Satureja from lamiaceae family consists of many species all over the world. In Iran, there are 15 species, 9 of them are endemic. The oil of different Satureja species are very important because of having carvacrol. In this study, the effect of two extraction methods (hydro-distillation and supercritical fluid extraction) on yield and composition of the essential oils Satureja hortensis and Satureja rechingeri wasinvestigated.
The aerial parts of S. hortensis were collected from Research Station of Alborz (Karaj) at full flowering stage and those of S. rechingeri were collected from their natural habitats on Ilam province. After drying the plant material, the oil of both species were obtained by hydro-distillation and also by SFE (with and without modifier). The oil were analyzed by GC and GC/MS. 17 compounds were identified in the oil of S. hortensis by hydro-distillation, with carvacrol (48.1%) and g-Terpinene (38.4%) as the main components. In the oils of SFE, 9 compounds were characterized with carvacrol (57.1%-63.4%) and g-Terpinene (27.0%-32.8%) as the major components. Therefore, the percentage of carvacrol was increased by SFE and g-Terpinene was decreased. 20 components were identified in the oil of S. rechingeri by hydro-distillation, with carvacrol (86.6%) as main constituent. In the oils of SFE, 6 compounds were identified, that the percentage of carvacrol (95.0%-95.6%) was higher. 

Keywords

- مظفری، و.، 1373. رده بندی گیاهی. نشر دانش امروز.
- میرزا، م.، سفیدکن، ف. و احمدی، ل.، 1375. اسانسهای طبیعی. انتشارات موسسه تحقیقات جنگلها و مراتع، تهران، 200 صفحه. 
- قهرمان، ا.، 1373. کوروموفیتهای ایران. مرکز نشر دانشگاهی، تهران.
- بقاییان نقدی، 1379. گیاهان اسانس دار. انتشارات نشر اندرز. 
- کمالی زاد، ع.، 1367. مبانی کروماتوگرافی گازی. انتشارات نشردانشگاهی، تهران.
 - Adams, R. P., 1996. Identification of Essential oil Components by Gas  Choromatography/Mass Spectroscopy, Allured publishing Corp., Carol Stream,USA.
- Davies, N. W., 1990. Gas chromatographic retention indices of monoterpens and sesquiterpenes on methyl silicone and carbowax 20 M phaseJ. Chromatography, 503: 1-24.
- Sefidkon, F., Dabiri, M. and Rahimi- Bidgoly, A., 1999. The effect of distillation methods and stage of plant growth on the essential oil content and composition of Thymus kotschyanus boiss. & Hohen, Flavour & Fragrance journal, 14: 405-408.
- Sefidkon, F. and Jamzad, Z., 2005. Chemical composition of the essential oils of three Iranian Satureja species (S. mutica, S. macratha and S. intermedia)Food Chemistry, 91: 1-4.
- Sefidkon, F., Jamzad, Z. and Mirza, M., 2004. Chemical variation in the essential oil of Satureja sahendica from IranFood Chemistry, 88: 325-328.
- Ebrahimzadeh, H., Yamini,Y., Sefidkon, F., Chaloosi, M. and Pourmortazavi, S., 2003. Chemical composition of the essential oil and supercritical CO2 extracts of Zataria multiflora Boiss., Food Chemistry,  83: 357-361.
- Rojas, L and Usubillaga, A., 2000. Composition of the essential oil of Satureja brownie (SW.) Briq. from Venezuela, Flavour & Fragrance Journal, 15: 21-22.
- Skocibusic, M. and Bezic, N., 2004. Phytochemical analysis and in vitro antimicrobial activity of two Satureja species essential oils, Phytothor Research, 18(12): 964-70.
- Leake, G., Gaspar, F. and Santos, R., 2003. Effect of water on the solubility of essential oils in dense CO2, Journal of Essential Oil Research, 15: 172-177.
- Versailles, F., 2003. Evaluation of some GC methods to predict the critical properties of aroma compounds. Proceeding of 6th International Symposiums on Supercritical Fluids.
- Sefidkon, F., and Jamzad, Z., 2004. Essential oil composition of Satureja spicigera (Koch) Boiss. from Iran, Flavour & Fragrance Journal, 19(6): 571-573.
- Simon, j. E., Chadwick, A. F. and Craker, L. E., 1984. Herbs: An Indexed Bioliography. 1971-1980. The Scientific Literature on Selected Herbs, and Aromatic and Medicinal Plant of the Temperate Zone. Archon books, 770 pp., Hamden, CT.
- Bahramifar, N., Yamini, Y. and Sefidkon, F., 2005. Comparison of Iranian satureja sahendica essential oil obtained by supercritical fluid extraction and hydrodistillation, Food chemistry, In Press.
- Baser, KHC, Ozek, T., Kirimer, N. and Tumen, G., 2004. A comparative study of the essential oils of wild and cultivated Satureja hortensis L., Journal of Essential Oil Research, 16 (5): 422-424.
- Ghannadi, A., 2002. Composition of the essential of Satureja hortensis L. seeds from Iran, Journal of Essential Oil Research, 14(1): 35-36.