G.M. Garivani; E. Sharifi Ashoorabadi; S. Safari; M. Mirza
Abstract
This research was aimed to evaluate the effect of cultivation and domestication of two thyme (Thymus L.) species, Khorasani (Thymu stranscaspicus) and garden (Thymus vulgaris), on growth and yield components. The study was conducted in different agricultural ecosystems of North Khorasan province during ...
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This research was aimed to evaluate the effect of cultivation and domestication of two thyme (Thymus L.) species, Khorasani (Thymu stranscaspicus) and garden (Thymus vulgaris), on growth and yield components. The study was conducted in different agricultural ecosystems of North Khorasan province during 2005-2009. The main treatments consisted of harvesting time in a randomized complete block design with three replications. Results showed that the number, order and extent of the main chemical compounds in both species of thyme did not differ much. However, the quantitative properties measured in both species were affected by different stages of harvest in each year. The highest dry weight in both species was obtained at the end of flowering stage in all years. Harvesting time had different effects on species composition of essential oil in the studied years. The highest efficiency of essential oil was recorded in mid-flowering stage especially in third year. The quantity of essential oil is the economic indicator of thyme performance. Garden thyme (T. vulgaris) produced more essential oil as compared to Khorasani thyme (T. transcaspicus).
K. Jaimand; M.B. Rezaee; Z. Behrad; M. Mirza; V. Mozaffarian; R. Azady; M. Naderi; M. Golipur; A. Bahmanzadegan; S. Meshkizadeh; Sh. Karimi
Abstract
Hypericum genus is one of the most important medicinal plants in Iran. Currently in Iran, there are 17 herbaceous, perennial and shrub species of Hypericum of which three species are endemic to Iran. This research was aimed to investigate Hypericine content in nine species of Hypericum. For Hypericine ...
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Hypericum genus is one of the most important medicinal plants in Iran. Currently in Iran, there are 17 herbaceous, perennial and shrub species of Hypericum of which three species are endemic to Iran. This research was aimed to investigate Hypericine content in nine species of Hypericum. For Hypericine content 1 gram of plant was extracted in two steps, chloroform extraction then methanol extraction using a Soxhlet device. Hypericin content was measured by HPLC, using the following condition, mobile phase: (methanol 68%, ethyl acetate 20% and sodium hydrosulphate (0.1 M) 12%) and stationary phase C18, and UV detector: set on 590 nm. Generally, no hypericin was detected in different organs of H. androsaemum L. Hypericine content detected in flowers, leaves and stems were: H. apricum Kar. & Kir. (in flowers 0.061% and leaves 0.005%), H.armenum Jaub. & Spach (flower 0.003%), H.asperulum Jaub. & Spach (in flower 0.025%, leaves 0.004% and stems 0.003%), in H.hirsutum L. (flower 0.007%), in H.linarioides Boss. (flower 0.007%), in H.tetrapterum Fries (flowers 0.008%, leaves 0.014%, and stem 0.001%), and H.vermiculare Boiss. & Hausskn. (flowers 0.005%), in H.perforatum L. (flowers 0.124%, leaf 0.028% , stem 0.003).
M. Lotfi; B. Abbaszadeh; M. Mirza
Abstract
In this study the effect of drought stress on morphological traits, metabolites proline accumulation, soluble carbohydrates and the yield of Tarragon (Artemisia dracunculus L.), was evaluated in order to produce Tarragon in drought stress condition as a medicinal plant, and also determine the threshold ...
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In this study the effect of drought stress on morphological traits, metabolites proline accumulation, soluble carbohydrates and the yield of Tarragon (Artemisia dracunculus L.), was evaluated in order to produce Tarragon in drought stress condition as a medicinal plant, and also determine the threshold of stress tolerance. This research was done using randomized complete blocks design with 4 treatments and 3 replications. The stress treatments were included T1 (100% of field capacity), T2 (80% of field capacity), T3 (60% of field capacity) and T4 (40% of field capacity). According to the results of statistical analysis, the drought stress had significant effect on morphological traits, flowering shoot yield, proline and soluble carbohydrates. In addition, the enhancement of drought stress led to decrease in plant height, canopy diameter, leaf length, leaf width and leaf surface, stem diameter, the length of longest lateral shoot, root length and its development, shoot yield and dry leaf yield. Comparison of treatment means showed that the highest plant height, canopy diameter, leaf length, leaf width and leaf surface, stem diameter, the length of longest lateral shoot, root length, shoot yield and dry leaf yield were related to T1 (non stress); in addition, the highest amount of proline, soluble carbohydrates and root development were related to T4 (40%FC). Therefore, drought stress had negative effect on most morphological traits and flowering shoot yield; however, it led to increase in the root length, proline and soluble carbohydrates of flowering shoots.
R. Kalvandi; M. Mirza; M. Atri; M. Hesamzadeh Hejazi; Z. Jamzad; K. Safikhani
Abstract
Thymus eriocalyx (Ronniger) Jalas belongs to the family Lamiaceae. Among different species of Thymus, distributed in the world, 18 species are growing in Iran, of which four species are endemic. To study the variation of essential oils among population individuals of this species in Iran D.S.S. (Determination ...
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Thymus eriocalyx (Ronniger) Jalas belongs to the family Lamiaceae. Among different species of Thymus, distributed in the world, 18 species are growing in Iran, of which four species are endemic. To study the variation of essential oils among population individuals of this species in Iran D.S.S. (Determination of special station) method was used. In this study, the aerial parts of 5 individuals of Thymus eriocalyx were collected from 10 special stations, at full flowering stage for chemical investigation and were dried at room temperature. The essential oil was extracted by hydrodistilation method using a Clevenger-type apparatus. The chemical composition of essential oils was analyzed by GC-FID and GC-MS. Results showed that the highest average yield of essential oil in three replications based on dry weight belonged to the first individual of Te2 population (4.09%), while the lowest was recorded for the second individual of Te10 population (0.21%). Totally, thirty one compounds were identified in the essential oil of 10 studied population individuals. In this study, it is noteworthy that the individuals of a population showed variation among themselves in terms of chemical compositions. Among existing chemical compounds in the essential oil of 50 individuals, the following compounds revealed the variation widely: p-cymene (0.1-20.1%), 1,8-cineole (0.4-29.4%), γ-terpinene (0.1-8.7%), linalool (0.1-82.3%), camphor (0.1-15%), borneol (1.6-22.7%), geraniol (0.1-74.6%), thymol (0.1-57.7%), geranyl acetate (0.1-49.6%), caryophyllene oxide (0.2-9.1%), limonene (0.2-24%) and α-terpineol (0.1-36.2%). The results of the analysis of chemical data using MVSP Software by UPGMA and PCO methods led to the identification of 9 chemotypes as follows of which 7 chemotypes were introduced as new chemotypes: 1-geraniol/geranyl acetate, 2-geraniol, 3- linalool/geraniol/geranyl acetate, 4-linalool,5-α-terpineol/1,8-cineole, 6-geranyl acetate, 7-geraniol/thymol/borneol/1,8-cineole, 8-thymol/limonene and 9-thymol/p-cymene/borneol.
A. Rahamani; M. Mirza; S.R. Tabaei-Aghdai
Abstract
In this project, the effect of chemical fertilizers, organic manure and their combination on the quality of essential oils of Damask Rosa was investigated in Karadj Alborz Research Station. The study was conducted using randomized complete blocks design with 20 independent treatments and three replications ...
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In this project, the effect of chemical fertilizers, organic manure and their combination on the quality of essential oils of Damask Rosa was investigated in Karadj Alborz Research Station. The study was conducted using randomized complete blocks design with 20 independent treatments and three replications in a loam-clay soil with pH =7.9. In this experiment, nitrogen, phosphorus and potash and a combination of trace elements as fertilizer and cow manure were used. Amount of essential oil and quality of essential oil at different treatments were measured in 3 years. Essential oils of petals were extracted by hydrodistillation method in three consecutive years and oil composition was identified in the first and second years of flowering stage using GC and GC/MS. The results of analysis of variance and mean performance showed that the difference among treatments was significant at 1% level of probability. Treatment N80P80K40 and 30 tons of manure per hectare had the highest amount of essential oil. The average amount of essential oil in three consecutive years in this with 0.35 percent was the highest and in control with 0.23 percent was the lowest value. The highest average oil yield in the three years was recorded for treatment N40P40K40, (1395.58 g/ha), and minimum was obtained for the treatment consisting only 40 tons of manure, (636.23 g/ha). After that, the minimum oil yield was recorded for control treatment (868.98 g/ha) in these three years. The composition of essential oils in the first year of flowering showed that Linalool, Geraniol and Citronelllol increased 88, 39 and 36 percent, respectively in treatment N40P40K40, compared with the control. In the second year of flowering, the amount of Geraniol in this treatment increased by 45 percent and that of hexadekanol and heptadekan decreased 45 percent and 30 percent compared with the control.
K. Jaimand; M.H. Assareh; M. Mirza; M. Nadery; Sh. Karimi; E. Parsa
Abstract
Eucalyptuses trees as the fast-growing forest species are native to the Pacific. This species were introduced to Iran more than 100 years ago and cultivated in the south, having perfect environment for them. This research was aimed to investigate seasonal fluctuations of essential oil and1,8-cineol content ...
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Eucalyptuses trees as the fast-growing forest species are native to the Pacific. This species were introduced to Iran more than 100 years ago and cultivated in the south, having perfect environment for them. This research was aimed to investigate seasonal fluctuations of essential oil and1,8-cineol content in Eucalyptus species of Fars province. The leaves of 14 Eucalyptus species were collected from Fars province in the middle of each season for two years (2007 & 2008). Afterward, essential oils were obtained by hydrodistillation method and compounds were identified by GC and GC/MS. Results showed that in each region, some of the species had the highest essential oil yield and quality; therefore due to the high essential oil content and 1,8-cineol, they could be recommended for large-scale cultivation in the region in order to extract essential oil. Studied Eucalyptus included: E. calcicultrix (Miq.) Blakely; E. camaldulensis var. camaldulensis Dehnh.; E. cornuta; E. fruticetorum; E. intertexta R.T. Baker; E. largiflorens F. Muell.; E. lesouefii; E. longicornis F. Muell.; E. oleosa F. Muell.; E. rudis Endl.; E. salmonophloia F. Muell.; E. salubaris; E. stricklandii Maiden; E. viridis. The essential oil yield of different species at different seasons differed from 1.0 to 6.8% in 2007 and 0.3 to 5.8% in 2008. According to the results, the highest oil yield in autumn (5.2%-6.4%), the highest percentage of 1,8-cineole in winter (91.4%-94%), and the best time to harvest were recorded for E. oleosa F. Muell at Shousni, Mamasani; therefore, E. oleosa F. Muell could be recommended for cultivation as the best species.
R. Kalvandi; S.M. Hesamzadeh Hejazi; M. Mirza; M. Atri; Z. Jamzad; K. Safikhani; M. Ahmadian
Abstract
In order to evaluate several ecological factors, morphological traits, essential oil productivity and ploidy levels of Thymus eriocalyx (Ronniger) Jalas with uses of DSS method ten natural habitats were selected in Lorestan, Markazi, Hamadan, Kermanshah and Kurdestan provinces in Iran. To ...
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In order to evaluate several ecological factors, morphological traits, essential oil productivity and ploidy levels of Thymus eriocalyx (Ronniger) Jalas with uses of DSS method ten natural habitats were selected in Lorestan, Markazi, Hamadan, Kermanshah and Kurdestan provinces in Iran. To study and evaluate morphological characters, five complete plant samples and flowering branches were collected from each locality at flowering stage for essential oil extraction in spring season of 2008. Ecological data of ten localities were also recorded. Vegetative and reproductive traits and essential oil quantity of each population were studied. Seeds were also collected in the seed production season to study seed characters, chromosome counting and ploidy level. At least 10 mitotic cells of each population were studied to determine chromosome numbers and ploidy levels. All populations were classified in 4 groups. Results revealed that specimens from "Te9 population" had the highest average length and width of inflorescence leaf, inflorescence leaf length to width ratio, stem leaf length to width ratio, petiole length, calyx tube length, corolla length and the highest average length and width of bracteole. The results of chromosome counting showed that specimens of Te1, Te2, Te3, Te4, Te5, Te6 and Te8 populations were diploid (2n=2x=30) and specimens of Te7, Te9 and Te10 populations were tetraploid (2n=4x=60). The highest essential oil production belonged to Te5 population (3.04%).
R. Kalvandi; M. Mirza; K. Safikhani; M. Naderi
Abstract
Rhabdosciadium aucheri Boiss., belonging to Apiaceae family, is an endemic species in flora of Iran that grows in Hamadan, Lorestan, Chaharmahale Bakhteyari, Kohkeyloye va Boyrahmad and Tehran. Aerial parts of Rhabdosciadium aucheri Boiss were collected at full flowering stage on July, 2010 from Iran: ...
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Rhabdosciadium aucheri Boiss., belonging to Apiaceae family, is an endemic species in flora of Iran that grows in Hamadan, Lorestan, Chaharmahale Bakhteyari, Kohkeyloye va Boyrahmad and Tehran. Aerial parts of Rhabdosciadium aucheri Boiss were collected at full flowering stage on July, 2010 from Iran: Hamadan, Asadabad at an altitude of 2330 m. Then, 100 g of air-dried aerial parts of plant was subjected to hydrodistillation. The essential oil yield was 0.67% based on the dry weight of plant. The oil was analyzed by GC and GC/Mass. Totally, eighteen constituents were identified. β-sesquiphellandrene (49.9%), epi-α-bisabolol (11.9%), intermedeol (8.20%), germacrene D (7.6%) and β-bisabolene (4.9%) were identified as the major constituents. This research was carried out for the first time in the world.
L. Safaei; E Sharifi Ashoorabadi; H. Zeinali; D. Afiuni; M. Mirza
Abstract
In order to study the effects of harvesting stages on essential oil yield and composition of Thymus caramanicus Jalas, an experiment was conducted during 2007-2009 in Fozveh Research Station of Isfahan. collected seeds from natural habitat were cultivated in pots and then seedlings were transplanted ...
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In order to study the effects of harvesting stages on essential oil yield and composition of Thymus caramanicus Jalas, an experiment was conducted during 2007-2009 in Fozveh Research Station of Isfahan. collected seeds from natural habitat were cultivated in pots and then seedlings were transplanted at the main field in a randomized complete blocks design with 3 replications. Aerial parts of the species were harvested at 4 stages including: beginning of flowering, fifty percent flowering, full flowering and fruit set during two years. The plant materials were dried and their essential oils were obtained by water distillation. Capillary GC and GC/MS analysis were used to determine the volatile profile of essential oil samples. Results showed that harvesting stages had significant effects on thymol and carvacrol content and essential oil percentage. The highest amount of essential oil and thymol percentage were obtained at full flowering stage of the first year (1.56% and 3.59 kg/ha, respectively) and the maximum carvacrol percentage was obtained at fruit set stage of the first year (25.45 kg/ha). Six components consisted the highest amount of essential oil, among them carvacrol was dominant at all 4 harvesting stages. The highest amount of carvacrol was obtained at the beginning of flowering stage (88.45%) in the second year. Thymol was the second dominant component with a maximum percentage of 11.77%, obtained at full flowering stage. The highest content of ρ- cymene, 1,8-cineole and γ-terpinene were obtained at fruit set stage (2.35, 1.21 and 2.15%, respectively) and the highest content of borneol was recorded at full flowering stage (7.52%). Also, a positive correlation was observed between essential oil percentage and amounts of thymol and carvacrol. In conclusion, the best time for harvesting of T. caramanicus to achieve the maximum carvacrol percentage was fruit set stage, while full flowering stage was identified as the best time to achieve the maximum essential oil and thymol percentage.
F. Askari; M. Mirza
Abstract
Centaurea depressa M. Bieb. (wheat flower) is a medicinal plant that its extraction is used for eye irrigation and somewhat reduces gastrointestinal irritation. This research was aimed to investigate the essential oil of C. depressa. Different organs of C. depressa were collected from Damavand and Tehran ...
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Centaurea depressa M. Bieb. (wheat flower) is a medicinal plant that its extraction is used for eye irrigation and somewhat reduces gastrointestinal irritation. This research was aimed to investigate the essential oil of C. depressa. Different organs of C. depressa were collected from Damavand and Tehran at flowering stage. The plant organs including stem plus leaf, root and inflorescence were separated and dried at laboratory temperature and then were ground to small particles. Essential oils were obtained by hydro-distillation method and analyzed by GC and GC/MS. The yield of essential oil of stem plus leaf, root and inflorescence of Damavand samples were 0.18%, 0.13% and 0.07% w/w (dried weight), respectively and for Tehran samples the values of 0.10%, 0.13% and 0.08% were recorded, respectively. In Damavand, the major constituents of essential oil obtained from stem plus leaf, root and inflorescence were thymol (56.5%), spathulenol (12.7%) and germacrene D (9.9%); pentadecadiene-1-ol (32.2%) and Z-7-hexadecene (29.5%); and germacrene D (32.4%), germacrene B (9.4%) and thymol (8.7%), respectively. In Tehran, the major constituents of essential oil obtained from stem plus leaf, root and inflorescence were Spathulenol (25.4%), Eudesms-4(15)-7-diene-1-b-ol (14.5%) and germacrene D (13.7%); tetradecanal (30.7%), caryophyllene oxide (23.7%) and Z-7-hexadecene (22.3%); and tetradecanal (82.3%) and Z-7-hexadecene (15.5%), respectively. According to the results, it can be concluded that there is variability in essential oil compositions of the two sites and even in different plant's organs.
M. Yousefi; V. Nazeri; M. Mirza
Abstract
Salvia leriifolia Benth. is one of the valuable medicinal plants of Lamiaceae. In order to evaluate genetic diversity among natural populations of Salvia leriifolia, 12 natural populations were chosen from South Khorasan, Razavi Khorasan and Semnan provinces. To study and evaluate 33 vegetative and reproductive ...
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Salvia leriifolia Benth. is one of the valuable medicinal plants of Lamiaceae. In order to evaluate genetic diversity among natural populations of Salvia leriifolia, 12 natural populations were chosen from South Khorasan, Razavi Khorasan and Semnan provinces. To study and evaluate 33 vegetative and reproductive morphological traits with five replications, 15 complete plants were collected from each locality at flowering and seeding stages. Flowering stems were used in order to extract the essential oil. Mean comparisons of data was performed by Duncan's multiple range test at 5% level of probability using SAS software. The populations were clustered by Ward method using SPSS software. Results showed that this plant grew well in a height of 1000-1533 above sea level, with an average temperature of 15-23ºcm and a precipitation of 80-194 mm in year. The most important morphological traits such as plant height, leaf length and width, were in the range of 18.9-35.8, 6.59-15.29 and 18.2- 29.7 cm, respectively. Maximum and minimum essential oil yield with 1.37 and 0.69 percent were obtained for Najmabad and Tabas Sabzevar, respectively. Populations of Salvia leriifolia were classified into three groups based upon collection locations. Results showed that "Najmabad" and "Bejestan" populations could be recommended for breeding and domestication programs because of appropriate morphological traits and abundant essential oil yield. Meanwhile, conservation of these populations is highly recommended.
M. Mirza; M. Najafpour Navaei
Abstract
The genus of Heracleum is from Umbelliferae with 10 species growing in Iran of which, four species including H. gorganicum Rech. F. are endemic. The aim of the current study was to determine the quantity and quality of fruit essential oil of the mentioned species collected from Golestan national park ...
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The genus of Heracleum is from Umbelliferae with 10 species growing in Iran of which, four species including H. gorganicum Rech. F. are endemic. The aim of the current study was to determine the quantity and quality of fruit essential oil of the mentioned species collected from Golestan national park at altitudes of 1400, 1700 and 2000 m. The essential oils were obtained by hydrodistillstion and analyzed by GC and GC/MS. Twenty-one components were characterized for the seed oil. Main components of all essential oils were recorded for octyl acetate (33.1%, 36.6% and 39.9%), Hexyl butyrate 22.5%, 25.6%, 29.8% and Hexyl 2-methyl butyrate 5.4%, 6.3% and 7.1% respectively at altitudes of 1400, 1700 and 2000 m. It is noteworthy to state that no reports have been published yet on essential oil of the studied species.
L. Safaei; E. Sharifi ashoorabadi; H. Zeinali; M. Mirza
Abstract
In order to study the effect of different harvesting stages on aerial yield, essential oil percentage and essential oil components in Thymus daenensis Celak., an experiment was conducted in Esfahan as a randomized complete blocks design with 3 replications during 2007- 2009 and the best harvest time ...
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In order to study the effect of different harvesting stages on aerial yield, essential oil percentage and essential oil components in Thymus daenensis Celak., an experiment was conducted in Esfahan as a randomized complete blocks design with 3 replications during 2007- 2009 and the best harvest time for maximum aerial yield, essential oil yield and amount of thymol were investigated. Aerial parts of the plants were harvested at four stages including: beginning of flowering, 50% flowering, full flowering and fruit set and were dried in the shade. The results showed that different harvesting stages had significant effects on all evaluated traits. According to the results of mean comparisons, the highest aerial dry weight and essential oil yield occurred at full flowering stage and the highest essential oil percentage was recorded at 50% flowering. Interaction effect of year and different harvesting stages showed that the highest aerial dry weight and essential oil yield (3083 and 68.61 kg/ha respectively) were recorded at full flowering stage in the second year. In both years, the highest amount of essential oil percentage was observed at 50% flowering and the year had no significant effect on this trait. The essential oil was mainly consisted of six components, among them thymol was dominant at four harvesting stages. The highest amount of thymol was obtained from beginning of flowering stage (85.9%) which had significant difference compared to other stages. The second dominant component was carvacrol at fruit set which showed significant difference compared to other stages. The maximum amount of P-cymene, 1,8-cineole and δ-terpinene were recorded at 50% flowering stage (3.4, 1.4 and 1.8% respectively). Borneole was also dominant (2.53%) at fruit set. In conclusion, full flowering stage was identified as the best time for harvesting of T. daenensis to achieve the maximum aerial yield, essential oil and thymol yield.
M. Mirza; F. Ghoraishi; A. Bahadori
Abstract
In this research, the effect of different harvesting stages on essential oils content and composition of Mentha piperita L. and Salvia officinalis L. was studied. The rhizome of Mentha piperita was planted at 5 cm soil depth in the field of "Jangalkar Ferdos Company", 2008. The salvia seeds were received ...
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In this research, the effect of different harvesting stages on essential oils content and composition of Mentha piperita L. and Salvia officinalis L. was studied. The rhizome of Mentha piperita was planted at 5 cm soil depth in the field of "Jangalkar Ferdos Company", 2008. The salvia seeds were received from Isfahan research center. Their seedlings were transplanted on the furrows of the main field. The experimental design was randomized complete blocks and the treatments included early, full and after flowering stages. The experiment included six treatments and three replications in a total of 18 experimental units. After drying the samples in room air condition and removing all woody parts, essential oils of both species were obtained by water-distillation method in three replications and then analyzed by GC & GC/MS. According to the results obtained from analysis of variance, the effect of different harvesting stages on oil yield of Mentha piperita (P<0.05) and Salvia officinalis (P<0.01) was significant. Comparison of treatment means showed that the highest percentage of essential oil (2/8%) was recorded in full flowering stage of M. piperita while S. officinalis had the highest percentage of essential oil in early flowering stage (3%), with a significant difference in comparison to the other stages. Analysis and Identification of essential oils components showed that the major constituents of essential oil in S officinalis were α-thujene (20/8%, 27/1%, 35/9%) camphor (29/2%, 14/6%, 17/2%) and β-thujene (15/1%, 14/6%, 4/1%) in early, full and after flowering stages respectively. The major constituents of essential oils in M.piperita at early, full and after flowering stages were menthol (27/7%, 26/9%, 27/0%), menthon (37/0%, 21/9%, 17/2%), and menthofuran (16/0%, 22/0%, 25/3%).
A. Akbarinia; E. Sharifi Ashoorabadi; M. Mirza
Abstract
Thymus daenensis Celak. is distributed in some areas of Iran such as Qazvin province. In order to evaluate essential oil yield and composition of T. daenensis an experiment was conducted in research farm of Qazvin Research Station based on randomized complete block design with three replications during ...
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Thymus daenensis Celak. is distributed in some areas of Iran such as Qazvin province. In order to evaluate essential oil yield and composition of T. daenensis an experiment was conducted in research farm of Qazvin Research Station based on randomized complete block design with three replications during 2006-2008. Aerial parts of the plants were dried in shadow and hydro distilled for obtaining their essential oils. Capillary GC and GC/MS analysis were used to determine the volatile profile of the essential oil samples. Fresh and dry herbage yields of T. daenensis were 9124-1568, 10250-2176 and 11337-2750 kg/ha in 2006, 2007 and 2008 respectively. Aerial parts yield were higher in the first cutting and showed decreased trend in latter cutting each year. The yields of fresh and dry biomass were 3498-800 kg/ha for the first cutting, 3015-720 kg/ha for the second cutting, 1947-508 kg/ha for the third cutting and 1976-504 kg/ha for the forth cutting. There was no difference in volatile oil percentage in different years, but there were significant difference between different cuttings. The oil yield was 2.83% at third cutting and 3.07% at first cutting. Thymol, γ-terpinene, ρ-cemene, metyl-eyther carvacrol and carvacrol were the main components of essential oil in all cuttings and years. Thymol percentage was varied from 69% (first cutting) to 76% (second cutting) in the essential oils of T. daenensis.
M. Najafpour Navaei; M. Mirza
Abstract
Scaligeria (Apiaceae) is represented in Iran by seven species, which is located in natural habitat. In this Research the fruits and leaves of Scaligeria assyriaca Freyn & Bornm. were collected from Khorasan province.The oil constituents from fruits and leaves of this plant have been studied by GC ...
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Scaligeria (Apiaceae) is represented in Iran by seven species, which is located in natural habitat. In this Research the fruits and leaves of Scaligeria assyriaca Freyn & Bornm. were collected from Khorasan province.The oil constituents from fruits and leaves of this plant have been studied by GC and GC/MS. The major constituents in leaf oil were found to be germacrene D (21.1%), β-caryophyllene (13.4%) and α-copaene (10.2%). In the fruit oil the main components were myristicin (24.3%), germacrene D (13.7%) and elemicin (11%). β-Sesquiphellandrene and kessanwere identified ranging (10% and 8.7%) and (7.4% and 9.5%) in leaf and fruit oils, respectively.
Z. Baher Nik; M. Teimori; M. Mirza
Abstract
In this research, the effects of different irrigation treatments on chemical constituents and antibacterial effects of theessential oil of P. argentatum were studied. The experimental design was randomized complete block design with four replications. Four irrigation treatments were determined, ...
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In this research, the effects of different irrigation treatments on chemical constituents and antibacterial effects of theessential oil of P. argentatum were studied. The experimental design was randomized complete block design with four replications. Four irrigation treatments were determined, consisting of: (a) control, which was irrigated to full field capacity (FC); (b) low water stress treatment (LS1=75% of FC); (c) moderate water stress treatment (LS2=50% of FC); (d) sever water stress (HS=25% of FC). Then the volatile constituents of the flowers were isolated and their quantities, qualities and antibacterial effect determined against four gram negative and four gram positive bacteria on the basis of disc-diffusion method. The result showed that not only the quantity of the essential oils but also the qualities varied. The percentage of α-pinene, β-pinene, γ-eudesmol and β-eudesmol has changed. The LS2 treatment of essential oils showed more antimicrobial activity against both gram positive and gram negative bacteria compared to other essential oils. This higher activity of essential oil may be related to higher amount of α-pinene and β-pinene in LS2. In addition gram positive bacteria were more susceptible than gram negative bacteria.
M. Naderi Hagibaghercandi; F. Sefidkon; M.R. Poorherave; M. Mirza
Abstract
Laurus nobilis L. (Lauraceae) is an evergreen tree and growing in favorable condition to a height of about 15-20 meter. This ornamental tree is indigenous to the Mediterranean area and southeast part of Europe. The essential oil obtained from Laurus nobilis has been used for anti larvae, rheumatic pains ...
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Laurus nobilis L. (Lauraceae) is an evergreen tree and growing in favorable condition to a height of about 15-20 meter. This ornamental tree is indigenous to the Mediterranean area and southeast part of Europe. The essential oil obtained from Laurus nobilis has been used for anti larvae, rheumatic pains and muscular convulsion. Laurel is usually considered as the natural source of this compound, used in the flavor, cosmetic and fragrance industries. In the present study the leaves of Laurus nobilis were collected from two locations (Botanical Garden of Noshahr and National Botanical Garden of Iran in Tehran) in March 2009 and dried in shade. In addition the green stems and fruits were collected from Noshahr. The essential oil of all samples were obtained by hydro-distillation and analyzed by capillary gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). The oil yields based on dry weight for stem, leaf and fruit are respectively 0.22%, 0.43% and 1.35% (for Noshahr samples) and 3.86% for the leaves of Tehran sample. Twenty-fore, 27, 37 and 20 components were identified in the essential oils of stem, leaf, fruit (for Noshahr) and leaf (for Tehran) of Laurus nobilis (representing 95%, 98.5%, 95.6% and 98.4% of the oils), respectively. The major components in the stem oil were 1,8-cineole (42.9%), α-terpinyl acetate (16.8%) and sabinene(4.7%). The main compounds in leaf oil were 1,8-cineole (58.2%), α-terpinyl acetate (10%) and sabinene(7.2%) and main compounds in leaf oil in Tehran sample were 1,8-cineole (47%), sabinene(13.9%) andα -terpinyl acetate (11.5%). The major components in fruit oil were E-β-ocimene (20.8%), 1,8-cineole (14.4%), α-terpinyl acetate (8.5%), germacrene B (7.8%), α-pinene (6.6%), germacrene D (6%), sabinene(5.4%) and β-pinene (5.1%). The results obtained from this study showed that the oil yields from the stem was lower than leaves but in both stem and leaf oils the major components were similar. The fruit oil contained high amount of E-β-ocimene.
M. Peyvandi; A. Rafati; M. Mirza
Volume 25, Issue 1 , May 2009, , Pages 75-84
Abstract
The purpose of this study was determining the effects of different nitrogen and phosphorus doses on the plant growth parameters, yields and essential oil composition of Artemisia annua L. The effect of nitrogen (urea 46%) and phosphorus (Triple super phosphate %48) at four levels (0, 40, 80, 120 kg/ha) ...
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The purpose of this study was determining the effects of different nitrogen and phosphorus doses on the plant growth parameters, yields and essential oil composition of Artemisia annua L. The effect of nitrogen (urea 46%) and phosphorus (Triple super phosphate %48) at four levels (0, 40, 80, 120 kg/ha) were examined. Experiments were designed based on Randomized CompleteBlock with 16 treatments and 3 replicates. Results indicated that differences between the average of height, number of branches and dry weight were significant at p≤0.05. The maximum of branches number and height of plant were gained in the N40P40 and N80P40 treatments. Increasing of P fertilizer more than 80 kg/ha decrease the growth parameters significantly. Essential oil of leaves at flowering stage was obtained by hydro-distillation. Percentage of essential oil showed significant increase in N40P0 and N80P0 treatments. Chemical compounds of leaf oil were identified by GC/MS. Twenty four components were found which the major compounds were artemisia ketone, camphor, 1,8-cineole, artemisia alcohol, viridiflorene and alpha-pinene
M. Habashi; M. Mirza; Y. Mostofi; K. Jaimand
Volume 24, Issue 4 , February 2009, , Pages 428-436
Abstract
The citron (Citrus medica L.) belongs to Rutaceae family. Citron peel oil is widely used in perfume industry, medicine, and food products. In this research the fruits of citron were collected from North of Iran in the fall and peel oil obtained by hydrodistillation and cold-press methods with the ...
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The citron (Citrus medica L.) belongs to Rutaceae family. Citron peel oil is widely used in perfume industry, medicine, and food products. In this research the fruits of citron were collected from North of Iran in the fall and peel oil obtained by hydrodistillation and cold-press methods with the extraction efficacy of 0.6% and 0.5% (w/w), respectively. The oils were analyzed by GC and GC-MS. Twenty-five components were identified in the hydrodistilled oil. The main components were limonene (58.3%), γ-terpinene (16.8%), geranial (6%) neral (4.8%), geranyl acetate (1.4%) and geraniol (1.36%). Twenty-three components were characterized in cold-pressed oil with limonene (63.7%), γ-terpinene (21.7%) and geranial (1.3%) as the main components.
M. Najafpour Navaei; M. Mirza
Abstract
Leonurus cardiaca L. is an aromatic and medicinal plant which could be found in high and mountainous parts of Iran. It is a considerable plant for its essential oil. In order to study the volatile constituents, its aerial parts were collected from Damavand, essential oil isolated by water steam distillation ...
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Leonurus cardiaca L. is an aromatic and medicinal plant which could be found in high and mountainous parts of Iran. It is a considerable plant for its essential oil. In order to study the volatile constituents, its aerial parts were collected from Damavand, essential oil isolated by water steam distillation (clevenger) and were analyzed by a combination of capillary GC and GC /MS. 37 compounds were identified representing 98.4 % of the oil. It was dominated by Germacrene-D (20.9%), α–humulene (15.3%) and β–caryophyllene (13.8%).
Z. Baher Nik; M. Mirza; B. Abbaszadeh; M. Naderi Hajy Bagher Candy
Volume 23, Issue 3 , November 2007, , Pages 315-322
Abstract
Since the relationship between soil and water status of plants and its effect on growth, propagation and metabolism process, the effect of water stress on Parthenium argetatum, was investigated. Irrigation treatments were selected based on different percentage of field capacity (FC), including (1)a control ...
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Since the relationship between soil and water status of plants and its effect on growth, propagation and metabolism process, the effect of water stress on Parthenium argetatum, was investigated. Irrigation treatments were selected based on different percentage of field capacity (FC), including (1)a control sample which was irrigated to full field capacity during the growing season (FC), (2) two moderate water stress treatments (LS1=75% and LS2=50% of field capacity) and (3) severe water stress treatments (HS=25% of field capacity). Results showed that plant RWC (relative water content) decreased from 65.8% to 42.8%. Also water stress induced high amount of sugar and proline. The amount of sugar was the highest in sever treatment (HS=2.9 mg/g FW) while it was the least in FC treatment (1.07 mg/g FW). The amount of proline was the highest in sever treatment (HS= 4.28 mM) while it changed from 1.1 in LS1, 1.56 in LS2 and the least (0.96 mM) in FC treatments.
M. Mirza; M. Najafpour Navaei
Volume 23, Issue 3 , November 2007, , Pages 375-381
Abstract
Rosa damascene is one of the aromatic and medicinal plants which its flowers are used for obtaining rose water by traditional and industrial methods. In addition of rose water, the flowers contain 0.02% essential oil which is called first oil or direct oil. This essential oil is very valuable but there ...
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Rosa damascene is one of the aromatic and medicinal plants which its flowers are used for obtaining rose water by traditional and industrial methods. In addition of rose water, the flowers contain 0.02% essential oil which is called first oil or direct oil. This essential oil is very valuable but there are many water soluble compounds which are solved in rose water and therefore they should be extracted by different distillation methods. In this research two methods for distillation of rose water were used. These methods were distillation at atmospheric pressure (760 Torr) and vacuum. At both methods, all physicochemical parameters should be controlled till the best essential oil is obtained. This essential oil is called water oil or second oil. Essential oils, extracted from rose water by two methods, were evaluated for their chemical composition by GC and GC/MS. Comparative study of main components of essential oil obtained by distillation at atmospheric pressure and vacuum showed that the percentage of alcohols have increased by vacuum method. The amount of 2-phenyl ethyl alcohol has increased from 5.6% to 7.5%, followed by citronellol from 59.5% to 61.2% and geraniol from 13.2% to 14.3%.
M. Mirza; Z. Baher Nik
Volume 23, Issue 2 , August 2007, , Pages 278-284
Abstract
Use of the essential oils of Salvia genus in medical, culinary, food and cosmopolitic products and their biological activities depends on the chemical constitutes, therefore based on the importance of these essential oils, for determination the quantity and quality of the essential oil of Salvia lachnocalyx ...
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Use of the essential oils of Salvia genus in medical, culinary, food and cosmopolitic products and their biological activities depends on the chemical constitutes, therefore based on the importance of these essential oils, for determination the quantity and quality of the essential oil of Salvia lachnocalyx Hedge, we collected the aerial parts of plant in May from Fars province. Then the essential oil was isolated by water distillation and analyzed by GC and GC/MS. The thirty-four compounds were identified in the essential oil concluded 99.7% of the total oil. The major components were bicyclogermacrene (31.3%), α-pinene (13.2%), sabinene (11.7%) and β-pinene (10.3%). Other constitutes were α-terpinyl acetate (7.0%), β-caryophyllene (7.4%), limonene (2.8%) and 1,8-cineole (2.3%).
F. Sefidkon; M.H. Assareh; M. Mirza; Z. Abravesh; M.H. Salehe Shushtari
Volume 23, Issue 1 , May 2007, , Pages 39-50
Abstract
In this investigation, the leaves of five Eucalyptus species as Eucalyptus microtheca F. Muell, E. gongylocarpa Blakely, E. gillii Maiden, E. woodwardi Maiden and E. salubris F. Muell were collected from two regions of Khuzistan province (Shushtar and Dezful, South Iran) in spring. After drying the plant ...
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In this investigation, the leaves of five Eucalyptus species as Eucalyptus microtheca F. Muell, E. gongylocarpa Blakely, E. gillii Maiden, E. woodwardi Maiden and E. salubris F. Muell were collected from two regions of Khuzistan province (Shushtar and Dezful, South Iran) in spring. After drying the plant materials in shade, their essential oils were obtained by hydro-distillation. The oils were analyzed by capillary gas chromatography, using flame ionization and mass spectrometric detection. The oils of E. microtheca were obtained at yields of 0.43% and 0.37% (w/w) from Shushtar and dezful, respectively. These yields were 2.37% and 1.78% for E. gongylocarpa, 3.5% and 3.9% for E. gillii, 1.18% and 1.13% for E. woodwardi, 2.05% and 1.02% for E. salubris. The main compounds of E. microtheca oils were 1,8-cineole (26.7% and 16.9%), P-cymene (14.8% and 20.4%) and a-pinene (12.5% and 12.0%) in the Shushtar and Dezful samples, respectively. The major constituents of E. gongylocarpa oils were 1,8-cineole (66.8% and 78.6%) and a-pinene (5.2% and 7.2%). The main components of E. gillii oils were 1,8-cineole (58.5% and 81.3%) and a-pinene (13.9% and 11.0%). The main compounds of E. woodwardi oils were 1,8-cineole (58.7% and 45.1%) and a-pinene (23.1% and 21.4%). The major constituents of E. salubrisoils were 1,8-cineole (62.0% and 71.0%) and a-pinene (8.0% and 10.0%). Differences in the percentage of the main components of these oils in two regions could be due to some differences in habitats like soil structure or humidity.
