F. Askari; M. Mirza; M. Golipour; S. Fekri Qomi
Abstract
The genus Achillea has 19 species of herbaceous, perennial and aromatic plant in Iran. This genus of compositae family has complex characteristics. Chamazolene is a major component of essential oil of inflorescence and leaf, reported to be used in food, cosmetic, and pharmaceutical industries. A. millefolium ...
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The genus Achillea has 19 species of herbaceous, perennial and aromatic plant in Iran. This genus of compositae family has complex characteristics. Chamazolene is a major component of essential oil of inflorescence and leaf, reported to be used in food, cosmetic, and pharmaceutical industries. A. millefolium L. subsp. elbursensis is an endemic subspecies of Iran and no studies have been conducted on the cultivation of this species; therefore a preliminary study was conducted on the cultivation and its effect on secondary metabolites. For this purpose, the seeds of Achillea were collected from Dizine area in October 2016. The seeds of Achillea were cultivated in a greenhouse at the beginning of March and seedlings were transplanted to the farm in late April 2017.At flowering stage in August, aerial parts were collected in two consecutive years to obtain the essential oils. To compare the essential oil of cultivated samples with habitat samples, the aerial parts of Achillea were collected from Dizin at the flowering stage in August 2017. The plant parts including leaf and inflorescence were dried in laboratory and were crushed to particles. The essential oils were obtained by hydrodistillation and were analyzed by GC-FID and GC/MS. The color of A. millefolium essential oils was dark blue. The yields of A. millefolium essential oils of leaf and inflorescence (w/w dried weight) from habitat samples were 0.11% and 0.53%, respectively, and from cultivated samples were 0.28% and 0.50% in 2017 and 0.26% and 1.30% in 2018, respectively. Chamazulene was the major constituent of leaf (5.7%) and inflorescence (52.5%) oils in the habitat samples. The content of this compound in cultivated samples was 50.6% and 67.1% in the first year, and 59.6% and 71.3% in the second year, respectively. Another major constituents were caryophyllene alcohol, caryophyllene oxide, camphor, borneol and b-eudesmol. Chamazulene as major compound of the oil, found in all aerial parts of cultivated samples, while, it was found only in the inflorescence of wild sample.
M.S. Dehghani; M. Naeemi; E. Gholamali Alamdari; H. Jabari
Abstract
In order to evaluate the effects of chitosan foliar application under water deficit stress conditions on quantitative and qualitative characteristics of German chamomile (Matricaria chamomilla L.), a study was conducted based on randomized complete blocks design with factorial arrangement of treatments ...
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In order to evaluate the effects of chitosan foliar application under water deficit stress conditions on quantitative and qualitative characteristics of German chamomile (Matricaria chamomilla L.), a study was conducted based on randomized complete blocks design with factorial arrangement of treatments and three replications at Gonbad Kavous University research field, Iran in 2014 growing season. Treatments included irrigation at two levels, irrigation after 60 mm evaporation from class A pan and 100 mm evaporation from class A pan, and chitosan spraying at five levels including non-application of chitosan (spraying with distilled water as control (K1)), chitosan spraying at 125 mg/l after 60 days of planting (K2), 125 mg/l after 75 days of planting (K3), 250 mg/l after 60 days of planting (K4) and 250 mg/l after 75 days of planting (K5). Results showed that water deficit stress decreased the plant height, number of flowers, plant dry weight and flower dry yield. Foliar application of chitosan increased the number of branches per plant and flower dry yield. Results indicated that utilization of second level of chitosan under stress and third level of chitosan under normal irrigation improved the chamazolen essential oil percentage and yield. According to results of this study, application of chitosan spraying at 125 mg/l after 60 days of planting under deficit water stress conditions caused the highest percentage and yield of essential oil and chamazulene percentage. In general, in order to prevent and reduce the damage of water stress as well as increased chamazulene, the use of bio-polymer chitosan as a natural material in German chamomile is important.
O. Mirzaee Cheshmehgachi; Y. Nasiri; E. Esfandyari; M. Nouraein
Abstract
Vitamins affect many metabolic and biological processes in plants and participate in the synthesis of enzymes, nucleic acids, and proteins as coenzyme in their metabolic pathways. In order to evaluate the effect of foliar application of thiamine on growth, yield, yield components and essential oil of ...
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Vitamins affect many metabolic and biological processes in plants and participate in the synthesis of enzymes, nucleic acids, and proteins as coenzyme in their metabolic pathways. In order to evaluate the effect of foliar application of thiamine on growth, yield, yield components and essential oil of fennel (Foeniculum vulgare Mill.), a field experiment was conducted as factorial on the basis of a randomized complete block design with three replications at the Faculty of Agriculture, University of Maragheh, Iran in 2015. The treatments were included three levels of thiamine foliar application (zero; distilled water), 50 and 100 μm/l) and foliar application stages in four levels of stem elongation, flowering, grain filing, and all three stages. The results showed that the effect of thiamine on all traits was significant. The concentration of 50 μm/l thiamine had the maximum increasing effect on the number of sub stems, number of umbels per plant, number of umbelets per umbel, number of grains per umbelet, grain yield, and essential oil yield and the concentration of 100 μm/l thiamine had the maximum increasing effect on the plant height, 1000-grain weight, biological yield, and essential oil percentage,compared to control. The effect of foliar application stages on the number of umbelets per umbel, number of grains per umbelt, and 1000-grain weight were significant and their highest values were obtained by thiamine foliar application in all three stages (stemming, flowering, grain filing). The interaction effects of the treatments were not significant on any of the traits. In general, results of this experiment showed that the foliar application of 50 and 100 μm/l thiamine at stemming, flowering, and grain filing stages can improve the seed yield and essential oil yield of fennel.
A. Rahemi Kahrizaki; R. Rahimi; A. Gholizadeh; E. Gholamalipour Alamdari; H. Saboori; S.H. Davoodi
Abstract
In order to investigate the effects of different levels of vermicompost and nitroxin on the qualitative properties of medicinal plant marigold (Calendula officinalis L.), an experiment was conducted as factorial based on a completely randomized design with four replications in the greenhouse of Gonbad-e-Kavos ...
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In order to investigate the effects of different levels of vermicompost and nitroxin on the qualitative properties of medicinal plant marigold (Calendula officinalis L.), an experiment was conducted as factorial based on a completely randomized design with four replications in the greenhouse of Gonbad-e-Kavos University during 2014-2015. The first factor was vermicompost fertilizer treatment at six levels (0%, 10%, 20%, 40%, 70% and 100%) and the second factor was biological nitroxin fertilizer at two levels of inoculation (25 ml/kg soil) and no inoculation with nitroxin. The results of analysis of variance showed that the effects of nitroxin, vermicompost and their interaction on all traits studied were significant. However, the total phenol content was only significant in the vermicompost treatment. The results showed that the highest total phenol content, chlorophyll a, and total chlorophyll were obtained in 10% vermicompost treatment with nitroxin inoculation. The highest chlorophyll b content was related to 10% vermicompost treatment without nitroxin inoculation. The highest nitrogen content in the plant and catalase content were obtained from 100% vermicompost treatment with nitroxin inoculation, and 100% vermicompost without nitroxin inoculation, respectively. The highest amount of essential oil was obtained from 100% vermicompost treatment with nitroxin inoculation and the lowest was obtained from control treatment without nitroxin inoculation. According to the results, 100% and 70% vermicompost with nitroxin inoculation could be suggested as a suitable treatment for the production of marigold essential oil.
H. Karami; M. Rasekh
Abstract
In this study, the thin layer drying kinetics of Tarragon (Artemisia dracunculus L.)was modeledin a hybrid dryer. Experiments were performed at four temperatures of 40, 50, 60, 70ºC and three air velocities of 1, 1.5 and 2 m/s in the factorial arrangement based on a completely randomized design. ...
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In this study, the thin layer drying kinetics of Tarragon (Artemisia dracunculus L.)was modeledin a hybrid dryer. Experiments were performed at four temperatures of 40, 50, 60, 70ºC and three air velocities of 1, 1.5 and 2 m/s in the factorial arrangement based on a completely randomized design. According to the results of analysis of variance, the effect of drying air temperature and drying air velocity were significant at 1% probability level. However, the interaction effect of temperature and drying air velocity was not significant. The highest essential oil content was related to the temperature of 40°C and an air velocity of 1.5m/s with an approximate value of 1.27CC (v/w). With increasing temperature from 40°C to 70°C, the essential oil content decreased significantly. According to the obtained results, the Agbashlo et al. model could estimate the kinetic curve of tarragon drying better than other models. The effective moisture diffusivity values were achieved to be in the range of 1.34×10−10-2.74×10−10 m2/s.
J. Hasani; M. Mirza
Abstract
This study was conducted to evaluate the essential oil (EO) quality and quantity of six thyme species (Thymus spp.), growing in the natural habitats of Kurdistan province, in 2010. To conduct this research, six species of thyme were identified in different habitats and used for essential oil extraction. ...
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This study was conducted to evaluate the essential oil (EO) quality and quantity of six thyme species (Thymus spp.), growing in the natural habitats of Kurdistan province, in 2010. To conduct this research, six species of thyme were identified in different habitats and used for essential oil extraction. Water distillation (Clevenger-type apparatus) was used to obtain the EOs and the components were identified using the gas chromatography (GC) system and a gas chromatograph connected to mass spectrometer (GC/MS). The results showed that more than 30 components were identified in the EOs, among which thymol, carvacrol, geraniol, alpha terpinole and linalool were the most important components. The EO yields were 3.05%, 2.53%, 2.83%, 1.51%, 1.31% and 1.05% for T. fallax, T. daenensis, T. pubescens, T. transcaucasicus, T. fedtschenkoi and T. kotschyanus, respectively. The content of thymol was calculated to be 70.6%, 62.2%, 27.8% and 1.5 in the EOs of T. daenensis, T. fedtschenkoi, T. pubescens and T. kotschyanus, respectivelyand the content of carvacrol was recorded to be 3.43%, 4.80% and 0.74% in the EOs of T. daenensis, T. fedtschenkoi and T. pubescens, respectively.
Sh. Abbasi; S. Houshmand; N. Mirakhorli; R. Ravash
Abstract
The leaves of thyme are the most important organs producing essential oils, and the glandular trichomes provide a unique capacity for the synthesis and storage of active ingredients. In this study, the morphological characteristics and types of trichomes' structure were measured in four thyme species ...
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The leaves of thyme are the most important organs producing essential oils, and the glandular trichomes provide a unique capacity for the synthesis and storage of active ingredients. In this study, the morphological characteristics and types of trichomes' structure were measured in four thyme species including: T. transcaucasicus, T. carmanicus, T. daenensis and T. armeniacus. The results of variance analysis indicated a significant difference between species in terms of stem length, leaf length and number, plant fresh and dry weight, leaf and stem dry weight, number of glandular trichomes on the adaxial and abaxial side of the leaf, the trichome length, and essential oil yield and percentage. T. armeniacus showed the highest trichome number; while, the highest trichome length was related to T. transcaucasicus. The highest essential oil percentage and yield were observed in T. carmanicus and T. daenensis respectively, which could be due to the greater number of glandular trichomes and leaves in these species. The results of scanning electron microscope (SEM) revealed that among various types of glandular trichomes, peltate glandular trichome existed in the leaf of four species examined. Positive and significant correlation coefficients between the number of glandular trichomes on the adaxial and abaxial side of the leaves with the essential oil percentage and yield indicate the role of this feature in the production of essential oil. The stepwise regression analysis, implemented for dry weight, indicated that the highest regression positive coefficient was observed for the leaf dry weight, explaining 97% of the trait variation. When the essential oil yield was assumed as a dependent variable, only the trichome length entered the model and explained 49% of the trait variation.
V. Rowshan; A. Bahmanzadegan Jahromi; F. Zareiyan; A. Hatami; L. Jowkar
Abstract
Marrubium astracanicum Jacq. is belonging to the Lamiaceae family. In this study, five populations of M. astracanicum (Derak, the west of Dasht Arjan, Bavanat-Simakan (Lakposhti), Ghorogh Abolmahdi (Pasargad) and Sadra areas) from Fars province were collected. After drying the plant samples, the essential ...
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Marrubium astracanicum Jacq. is belonging to the Lamiaceae family. In this study, five populations of M. astracanicum (Derak, the west of Dasht Arjan, Bavanat-Simakan (Lakposhti), Ghorogh Abolmahdi (Pasargad) and Sadra areas) from Fars province were collected. After drying the plant samples, the essential oils (EOs) were extracted from the aerial parts by using the Clevenger Apparatus, and analyzed by Gas Chromatography (GC-FID) and Gas Chromatography/Mass Spectrometry (GC/MS). Methanolic extract was prepared by maceration method and analyzed by HPLC for polyphenolic compounds. EO yields were from 0.21 to 0.34% (w/w) based on dried material. The major constituents of the EOs were n-decane, n-dodecane, germacreneD, n-hexadecanoicacid, (E)-caryophyllene, bicyclogermacrene, phytol, n-tricosane, n-pentacosane, n-heneicosane and caryophyllene oxide. The 12 major compounds were analyzed by Minitab V.14 software. The findings of chemical compounds showed that despite the difference in height and climate, Sadra region was more similar to Derak and Lakposhti Bavanat. The standards of gallic acid, quercetin, catechin, caffeic acid chlorogenic acid were measured in the wavelengths of 280nm and and 320nm. The amount of polyphenols varied in different areas. Our results showed that the height and climate in each area caused the variation of polyphenolic compounds.
M. Rostaei; S. Fallah; A. Abbasi Sorki; A. Tadayon
Abstract
Organic manure can be used as an alternative for chemical fertilizers in sustainable agriculture. Hence, compared with sole cropping systems, intercropping is a promising approach for crop production due to the lesser reliance on chemical fertilizer. In order to evaluate the effects of intercropping ...
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Organic manure can be used as an alternative for chemical fertilizers in sustainable agriculture. Hence, compared with sole cropping systems, intercropping is a promising approach for crop production due to the lesser reliance on chemical fertilizer. In order to evaluate the effects of intercropping on yield and essential oil of dill (Anethum graveolens L.) under organic manure and chemical fertilizers, a factorial experiment was conducted in a randomized complete block design with three replications at Shahrekord University research farm in 2016. Sole cropping of soybean (S) and dill (D) accompanied by three intercropping ratios of them (S:D 2:1, S:D 1:1, and S:D 1:2) were evaluated as the first factor, and two sources of fertilizer (chemical fertilizer, and broiler litter) as the second factor. The results showed that the highest dry matter yield of dill (1001.48 kg ha-1) and essential oil yield of dill (18.47 kg ha-1) was observed in the one-row dill + two-row soybean. The maximum essential oil percentage of dill (2.64%) was achieved in sole crop under organic manure. The highest pod/ plant (47.50) and seed yield of soybean (2192 kg ha-1) were dedicated to the two-row dill+one-row soybean under organic manure. The maximum land equivalent ratio (1.19) was achieved in treatment of one-row dill + two-row soybean under organic manure. According to the results, treatment of one-row dill + two-row soybean under organic manure had the highest land equivalent ratio, and produced a large amount of dill essential oil; therefore, it was considered as the superior treatment.
M.S. Hosseini; S. Mohammadi; A.R. Eftekhariyan Jahromi
Abstract
Mold decay is one of the most important diseases after harvesting citrus fruits, produced by Pencillium italicum fungi. It annually causes high amount of losses in citrus products. The essential oil of coca bush, Francoeuria undulata (L.) Lack, has high antibacterial properties on microorganisms. ...
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Mold decay is one of the most important diseases after harvesting citrus fruits, produced by Pencillium italicum fungi. It annually causes high amount of losses in citrus products. The essential oil of coca bush, Francoeuria undulata (L.) Lack, has high antibacterial properties on microorganisms. Therefore, in two separate experiments, the effect of various concentrations of essential oils (four levels including 0, 400, 800 and 1600) on inducing resistance of orange's fruit was evaluated against blue mold (two levels: without pathogen and with pathogen) via studying the defense enzymes. The present study was conducted as factorial based on completely randomized design with three replications. In second experiment, the effect of essential oil on pathogen control was studied at concentrations of 0, 400, 800, and 1600 in a completely randomized design. Results of analysis of variance showed that the interaction of concentration×pathogen at 48 and 96- hour time periods was significant on three enzymes including catalase, peroxidase and phenol. Also, the results of analysis of variance showed that the effect of coca bush essential oil concentration on inhibitory of pathogen growth on fruit was significant. The interaction of pathogen× plant type showed that the highest content of phenol, peroxidase and catalase was associated with control treatments, while high phenol content was observed in pathogenic treatments with coca bush. The interaction of presence and absence of pathogen×concentration showed that the highest phenol content was obserevd in control treatments at 0 and 800 ppm concentrations. Peroxidase content was high in healthy treatments, while there was no difference among various treatments in terms of catalase content. The interaction of plant type×essential oil concentration showed that there was no significant difference among various treatments in terms of phenol, peroxidase and catalase content.
Z. Nasiri; A.R. Farokhzad; M. Fattahi
Abstract
In this study, 19 wild-grown populations of Prangos (P. acaulic, P. ferulacea, P. uloptera andP. asperula) from North-West of Iran were collected and total phenol and flavonoid content, antioxidant activity, chlorophyll a and b, total carotenoid and essential oil content were evaluated. According to ...
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In this study, 19 wild-grown populations of Prangos (P. acaulic, P. ferulacea, P. uloptera andP. asperula) from North-West of Iran were collected and total phenol and flavonoid content, antioxidant activity, chlorophyll a and b, total carotenoid and essential oil content were evaluated. According to the results, the highest total phenol content (12.5 mg GAE/g DW) was recorded in the population of Ghoshchi from P. uloptera collected from West Azarbaijan province and the lowest content (4.18 mg GAE/g DW) was observed in Showt population (P. Ferulacea), collected from Showt, West Azarbaijan. The highest (5.51mg/g DW) and lowest (1.2 mg/g DW) amount of total flavonoid content recorded in the population of Baneh from Kurdistan province (P. Ferulacea) and Naghadeh population of West Azarbaijan (P. asperula), respectively. In addition, the highest level of chlorophyll a (0.41 mg/g DW) and b (0.69 mg/g DW) was recorded in the population of Showt3 (P. uloptera), located in West Azarbaijan province, and the highest level of carotenoid (61.36 mg/g DW) was observed in Saqqez population (P. ferulacea) from Kurdistan province. The highest antioxidant activity (77.08 %) was obtained in the Showt population (P. Ferulacea). The highest percentage of essential oil was observed in the population of Maku (P. acaulic) and Showt2 (P. ferulacea) collected from West Azarbaijan province. According to the results of cluster and factor analysis, there were high phytochemical variations in different populations collected from different regions of the North-West of Iran, which can be used in breeding programs of this plant.
Sh. Tajmirali; Mahbubeh Setorki; Z. Hooshmandi
Abstract
Given the role of inflammation and pain, causing delayed diseases recovery, as well as the side effects of chemical drugs and traditional uses of Satureja bachtiarica Bunge, this study was aimed to evaluate the analgesic and anti-inflammatory effects of S. bachtiarica essential oil. In this experimental ...
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Given the role of inflammation and pain, causing delayed diseases recovery, as well as the side effects of chemical drugs and traditional uses of Satureja bachtiarica Bunge, this study was aimed to evaluate the analgesic and anti-inflammatory effects of S. bachtiarica essential oil. In this experimental study, analgesic and anti-inflammatory effects of S. bachtiarica essential oil were evaluated using acetic acid and xylene tests. In each test, male NMRI mice were randomly divided into five groups including control (normal saline, IP), positive control (indomethacin at a dose of 10 mg/kg/dexamethasone at a dose of 2mg/kg, IP) and intervention groups (Satureja bachtiarica essential oil at doses of 50, 75 and 100 mg/kg, IP). The collected data were analyzed using SPSS software. According to the results, S. bachtiarica essential oil at doses of 25, 50 and 100 mg/kg and indomethacin caused significant analgesic effects compared to the control group (P<0.001), and higher analgesic effect was recorded for the dose of 100mg/kg showed. In xylene test, inflammation in groups receiving S. bachtiarica essential oil at doses of 25, 50 and 100 mg/kg and dexamethasone was significantly lower than that of control group (P<0.001). Our results clearly showed the positive effects of Satureja bachtiarica essential oil in reducing inflammation and pain.
S.R. Tabaei-Aghdaei; M. Abootorabi Najafabadi; M.H. Lebaschi; A. Najafi-Ashtiani; A.A. Jafari; F. Sefidkon; L. Mirjani
Abstract
To evaluate biomass and oil percentage at flowering stage in different accessions of medicinal plants Satureja spicigera (C. Koch) Boiss. (spic1, spic2, spic3)and S. sahendica Bornm. (sah1, sah2, sah3, sah4, sah5, sah6)under dry farming condition, an experiment was carried out based on three-replicated ...
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To evaluate biomass and oil percentage at flowering stage in different accessions of medicinal plants Satureja spicigera (C. Koch) Boiss. (spic1, spic2, spic3)and S. sahendica Bornm. (sah1, sah2, sah3, sah4, sah5, sah6)under dry farming condition, an experiment was carried out based on three-replicated randomized complete block design in Damavand, Iran in 2014. Variance analysis showed significant differences (P<0.01) between two species for all the traits with the higher values for S. spicigera. Based on means comparison, higher dry shoot weight per plant(108.5 g) was detected in S. spicigera compared to S. sahendica with 25.82g. The highest amount of dry shoot weight per plant was obtained from spic2 accession (183.5g) in S. spicigera and from sah2 (45.51 g) in S. sahendica.Also, S. spicigera showed higher oil content (2.65%) compared to S. sahendica with 1.34%. Spic2 with the highest amount of oil (2.90%, 36.10 kg/ha) could be the best accession for cultivation under dry farming condition of Damavand. Considering lower plant canopy diameter in S. sahendica (32.19 cm) compared to S. spicigera (54.90cm), biomass and oil yield increase is possible with higher plant density. Furthermore, considering dry shoot weigh, plant height, plant canopy and day to flowering as selection indices, sah2 could be proposed as superior genotype of S. sahendica for dry land farming in Damavand or similar climates.
A. Poshtdar; A.R. Abdali Mashhadi; F. Moradi; S.A. Siadat; A. Bakhshandeh
Abstract
A range of methods are applied to enhance secondary metabolism in medicinal plants. Treatment of the plants with elicitors is one of these approaches. Salicylic acid (SA) exerts a positive impact on plant growth and yield. The present study, as a biannual field experiment, investigates the response of ...
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A range of methods are applied to enhance secondary metabolism in medicinal plants. Treatment of the plants with elicitors is one of these approaches. Salicylic acid (SA) exerts a positive impact on plant growth and yield. The present study, as a biannual field experiment, investigates the response of peppermint (Mentha piperita L.) to different rates of SA under different regimes of nitrogen nutrition during years of 2014-2015. The experiment was made as a random complete block design based on split-plot with three replicates. The treatments included nitrogen fertilization rate as the main factor (0, 70, 140, 210, and 280 kg N ha-1 applied as urea) and salicylic acid, as the sub factor, was spayed at different concentrations (0, 100, 200, and 300 μM). In both years, the highest nitrate reductase activity was found in the roots of the plants treated with 200 μM SA and fertilized with 280 kg N ha-1. Interestingly, the foliar activity of the enzyme was influenced only by nitrogen, whereas the highest rate of activity was determined using 280 kg N ha-1 (2.87 and 2.82 μM nitrite g-1 FW h-1, respectively in the first and second year). Higher concentration of SA caused to increased content of nitrogen and free amino acid in the plants fertilized with higher rates of nitrogen. Nitrate, total phenol, and soluble carbohydrates content of leaf was reduced in both years as the result of high rate of nitrogen and high concentration of SA applied. In the first and the second year, the highest fresh yields (3316.2 and 3480.7 g m-2, respectively), the highest dry yields (811.7 and 855.6 g m-2, respectively) and essence (13.01 and 15.2 mL m-2, respectively) were obtained through annual application of 210 kg N ha-1. Collectively, the application of 210 kg N ha-1 together with SA (200μM) is recommended to achieve desired quantitative and qualitative yield.
A. Vaez Shahrestani; F. Sefidkon
Abstract
The genus Achillea, with 19 different species, is a perennial and herbaceous plant with aromatic properties. Achillea biebersteinii Afam. has medicinal properties and commonly used as a medicinal plant. Different parts of Achillea have anti-bacterial, anti- inflammation, anti- allergy and antioxidant ...
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The genus Achillea, with 19 different species, is a perennial and herbaceous plant with aromatic properties. Achillea biebersteinii Afam. has medicinal properties and commonly used as a medicinal plant. Different parts of Achillea have anti-bacterial, anti- inflammation, anti- allergy and antioxidant usage in tradition and modern medicine. In order to compare the quality and quantity of essential oils of flowering shoot, the seeds of Achillea were collected from Golestan province and cultivated in the farm of Abolrz Research Station. Aerial parts (flower, leaf and shoot) were separately collected and dried in shade condition. The essential oils were extracted using hydro-distillation method and analyzed by GC and GC/MS. The highest and lowest essential oil yield was recorded for leaf (0.74%) and shoot (0.03%), respectively. The total essential oil yield of flowering shoots and flowers were 0.6% and 0.34%, respectively. The major components in the shoot essential oil were 1,8-cineole (15.1%), camphor (24.0%); in leaves:1,8-cineole (40.4%), artemisia ketone (28.1%), camphor (10.3%); in flowers: 1,8-cineole (41.3%), (24.3%), camphor (15.6%); and in flowering shoots: 1,8-cineole (35.4%), artemisia ketone (30.6%), camphor (16.2%). Based on the results of this research, the highest amount of artemisia ketone and camphor were found in the flowering shoots and the highest amount of 1,8-cineole in leaves and flowers.
A. Mohammadi; M. Amini Dehaghi; M.H. Fotokian
Abstract
In order to study the application of humic acid and different irrigation regimes on quantitative and qualitative characteristics of three accession of cumin (Cuminum cyminum L.), an experiment was conducted at the Research Farm of Medicinal Plants, Faculty of Agriculture, Shahed University in 2016. This ...
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In order to study the application of humic acid and different irrigation regimes on quantitative and qualitative characteristics of three accession of cumin (Cuminum cyminum L.), an experiment was conducted at the Research Farm of Medicinal Plants, Faculty of Agriculture, Shahed University in 2016. This experiment was a factorial split plot based on randomized complete block design with three replications. Experimental factors included three irrigation regimes (full irrigation, irrigation after flowering stage and irrigation after grain filling stage), foliar application of humic acid with two levels (0 and 200 mg/lit) and three cumin accessions (Esfahan, Kashan and Sabzevar). The results of analysis of variance showed that the interaction effect of irrigation regime*humic acid*accession had significant effect on single plant dry weight, grain yield, harvest index, chlorophyll a and total. The results showed that the highest single plant dry weight and grain yield were obtained in complete irrigation and humic acid application. Kashan accession had the highest branch number per plant, dry weight per plant, number of seeds per umbrella, grain yield and biological yield. The highest percentage of essential oil yield was observed in Sabzevar accession. The highest grain yield (533.3 kg/ha) was obtained in complete irrigation with the use of humic acid in Kashan accession. The highest average dry matter yield (230 kg/ha) was obtained in complete irrigation with the use of humic acid for Sabzevar accession. The highest amount of chlorophyll a and total was observed in irrigation after flowering stage and lack of application of humic acid in Sabzevar accession. The application of humic acid was not significant on the traits evaluated. The most efficiency of humic acid was obtained in full irrigation. According to the results of this experiment, it can be stated that full irrigation and application of humic acid can increase the yield of Kashan accession in similar experimental conditions.
M.H. Lebaschi; E. Sharifi Ashoorabadi; M. Makizadeh Tafti; S. Asadi-Sanam; Kh. Karimzadeh Asl
Abstract
In order to evaluate the effect of plant density on quantitative and qualitative yieldof three species of Thymus under dry farming conditions in Tehran (Damavand), Kurdistan and Kermanshah provinces, an experiment was conducted in a randomized block design based on split plot with three replications ...
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In order to evaluate the effect of plant density on quantitative and qualitative yieldof three species of Thymus under dry farming conditions in Tehran (Damavand), Kurdistan and Kermanshah provinces, an experiment was conducted in a randomized block design based on split plot with three replications during three years (2009-2011). The treatments were included three species (T. vulgaris L., T. daenensis Celak.and T. kotschyanus Boiss. & Hohen.) and three planting densities (4, 6 and 8 plant/m2), which were considered as main and sup-plots, respectively. Shoot dry matter yield, essential oil content, yield and essential oil components were determined. Results showed that the four-interaction effect (density, species, year and province) was significant on dry matter content, calculated to be from 75 to 5172 kg/h in different treatments. The maximum dry matter yield was observed at a density of 6 plant/m2 for T. daenensis in Kermanshah in the third year of experiment. The highest essential oil yield was obtained at a density of 8 plant/m2 for T. daenensis in Kermanshah in the second year of experiment. The maximum thymol content (80.33%) was related to T. daenensis inDamavand at a density of 6 plant/m2 in the third year of experiment. Overall, high production capacity of T. daenensis in the study provinces indicates the adaptation and economic yield of this native and valuable species.
F. Keneshloo; F. Sefidkon; H. Keneshloo; M.A. Alizadeh
Abstract
In order to study the essential oil content and composition of different accessions of Anthemis haussknechtii Boiss. & Reut, the seeds were collected from Kordestan, west Azarbaijan, Ilam and Fars provinces and sown at Alborz research station during 2013-2014. In the flowering stage, the flowering ...
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In order to study the essential oil content and composition of different accessions of Anthemis haussknechtii Boiss. & Reut, the seeds were collected from Kordestan, west Azarbaijan, Ilam and Fars provinces and sown at Alborz research station during 2013-2014. In the flowering stage, the flowering shoots were harvested and air died. The essential oil was obtained by hydro-distillation method and analyzed and identified by GC and GC/MS. Results showed that the essential oil yield of Piranshar and Darehshahr was 0.2% and 0.03%, respectively. Twenty eight components were identified in eight accessions and differed in number, type and percentage. The main components of essential oils were spathulenol in Darehshahr, Ghorveh, Divandareh2, Ivan, Piranshahr and Sanandaj (19.1, 18.1, 16.2, 14.5, 13.8 and 12.2 percent, respectively), caryophyllen oxide in Darehshahr, Ghorveh, Divandareh2, Fars and Divandareh1 (20.5, 13.2, 12.9, 12.4 and 12.2 percent, respectively) and globulol in Ivan, Divandareh2 and Ghorveh (12.0, 11.5 and 11.2 percent, respectively). Cluster analysis showed that eight essential oils of different populations were classified into three categories including Piranshahr-Fars, Ghorveh-Darehshahr and others. According to the results, to obtain some high-percentage components, it is essential to extract the essential oil from a specific population.
A. Afkari
Abstract
In order to investigate the effects of drought stress and nitrogen fertilizer on some physiological and biochemical characteristics of basil (Ocimum basilicum L.), a split-plot experiment was conducted based on randomized complete block design in three replications in the research farm of Islamic Azad ...
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In order to investigate the effects of drought stress and nitrogen fertilizer on some physiological and biochemical characteristics of basil (Ocimum basilicum L.), a split-plot experiment was conducted based on randomized complete block design in three replications in the research farm of Islamic Azad University, ArdabilBranch, 2015. The treatments included drought stress (70, 140 and 210 mm of class A evaporation pan) and nitrogen fertilizer at three levels (0, 50 and 100 kg/hectare) respectively. The results showed that the effect of drought stress treatment was significant on the traits investigated. The interaction of drought stress × nitrogen fertilizer had a significant effect on the traits measured, such as chlorophyll a, total chlorophyll, relative water content, dry matter yield, and essential oil yield. In addition, fertilizer treatment caused a significant increase in the physiological characteristics, dry matter yield, and essential oil yield. According to the results, the maximum content of chlorophyll a (2.65 mg/g fresh weight), total chlorophyll (3.42 mg/g fresh weight), relative water content (72.02 percent), dry matter yield (2261.25 kg/hectare), and essential oil yield (7.13 kg/hectare) were obtained for the control treatment and 100 kg of nitrogen per hectare. The maximum content of carbohydrates (3.40 μg/g glucose per fresh weight) and proline (0.681 µmol/g fresh weight) was related to the severe stress treatment. The content of chlorophyll a, b, total, relative water content, carotenoid, and essential oil percent and yield decreased by increasing the drought stress, while the content of carbohydrate and proline increased. Therefore, irrigation at 70 mm evaporation and 100 kg nitrogen fertilizer per hectare could be recommended as the best treatment to achieve the highest levels of chlorophyll a, chlorophyll total, relative water content, dry matter yield, and essential oil yield of basil.
A. Farhadi; M. Daneshvar; H.R. Eisvand; F. Nazarian Firoozabadi
Abstract
In order to study the effects of different amounts of humic acid fertilizer on morphological characteristics, yield components and essential oil yield of German chamomile (Matricaria chamomilla L.) under low irrigation stress, a research was carried out in the Agricultural Research Farm of Lorestan University ...
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In order to study the effects of different amounts of humic acid fertilizer on morphological characteristics, yield components and essential oil yield of German chamomile (Matricaria chamomilla L.) under low irrigation stress, a research was carried out in the Agricultural Research Farm of Lorestan University during 2016-2017. The experiment was conducted in a split plot based on randomized complete block design with three replications. The experimental factors included irrigation at three levels (irrigation after 30, 60 and 90 mm evaporation from class A evaporation pan as the main factor) and consumption of humic acid at three levels (0, 5 and 10 kg /ha as sub factor). The results showed that with increasing irrigation interval, the flower fresh weight, flower dry weight, receptacle diameter, stem height and stem diameter were significantly decreased. With increasing levels of humic acid, flower harvest index, root dry weight, biological yield, essential oil yield, chamazulene yield, chlorophyll content (a, b and total) showed significant increase at all irrigation levels. In the case of flower harvest index, there was no significant difference in irrigation after 90 mm evaporation in humic acid treatments. The highest seed yield was obtained in irrigation after 60 mm evaporation and using 5 kg/ha humic acid. The highest yield of essential oil and chamazulene was obtained in irrigation after 60 mm evaporation and using humic acid at a rate of 10 kg/ha. According to the results, irrigation after 60 mm evaporation with 10 kg of humic acid per hectare is suggested for the production of maximum essential oil and chamazulene. As well, the same irrigation level with 5 kg/ha humic acid could be recommended to produce maximum seed yield.
M. Mirza; M. Najafpour Navaei; M. Dini
Volume 20, Issue 4 , January 2018, , Pages 417-423
Abstract
Scutellariais a member of Labiatae, distributed in mediteranean region and south of Asia. In Iran 20 species are present in center, northern, northwest and western districts. The aerial parts of Scutellaria pinnatifida. were collected from Touchal in Tehran province at full flowering stage ...
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Scutellariais a member of Labiatae, distributed in mediteranean region and south of Asia. In Iran 20 species are present in center, northern, northwest and western districts. The aerial parts of Scutellaria pinnatifida. were collected from Touchal in Tehran province at full flowering stage in May 2004. Essential oil was isolated by hydrodistillation from the aerial parts of plant and analysed by a combination of capillary GC and GC/MS. The yeild of essential oil was obtained in 0.07% (w/w). 29 compounds in the oil have been identified representing 96.6% of the oil. The main constituent was germacrene D (39%). Other main components were β-caryophyllene, farnesene and bicyclogermacrene.
F. Sefidkon; Z. Jamzad; M.M. Barazandeh
Volume 20, Issue 4 , January 2018, , Pages 425-439
Abstract
The genus of Satureja with the persian name of “Marzeh” consists of 14 species in Iran, 9 of them are endemic. One of these endemic species is Satureja bachtiarica. In this research, the aerial parts of S. bachtiarica were collected from two different localities in Fars and Yazd provinces, ...
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The genus of Satureja with the persian name of “Marzeh” consists of 14 species in Iran, 9 of them are endemic. One of these endemic species is Satureja bachtiarica. In this research, the aerial parts of S. bachtiarica were collected from two different localities in Fars and Yazd provinces, at full flowering stage. The plant materials were hydro-distilled for obtaining their essential oils. The oils were analyzed by capillary GC and GC/MS. The oil yields were calculated equal to 2.15% for Yazd sample and 1.65% for Fars sample. Twenty compounds were identified in the oil of Fars sample with carvacrol (49.3%), p-cymene (12.7%) and trans-a-bergamotene (5.8%) as main components. Twenty compounds were characterized in the oil of Yazd sample with carvacrol (66.5%), p-cymene (15.2%) and linalool (4.6%) as main constituents. The relatively high amount of oil yield and high percentage of carvacrol in the oil of S. bachtiarica, showed that this species could have the medicinal and nutritional uses like S. hortensis and S. montana. These two Satureja species are not native of Iran and S. bachtiarica may be used instead of them.
M.H. Asareh; M.M. Barazandeh; K. Jaimand
Volume 20, Issue 4 , January 2018, , Pages 469-476
Abstract
Eucalyptus porosa was collected from Dezfool (Fadak Garden) in Martch 2004.Dry leaves of it were hydrodistilled in a clevenger type apparatus for 20 min. to produce an oil at the yield of 0.57%(based on dry leaves).In order of quantitavely and qualitavely analyses, the oil was injected to GC.Among Twenty-one ...
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Eucalyptus porosa was collected from Dezfool (Fadak Garden) in Martch 2004.Dry leaves of it were hydrodistilled in a clevenger type apparatus for 20 min. to produce an oil at the yield of 0.57%(based on dry leaves).In order of quantitavely and qualitavely analyses, the oil was injected to GC.Among Twenty-one compounds which were identified in the oil, 1,8-cineole (58.6%), α–pinene(12.8%) and nopinone (3.11%) were the major constituents, respectively.
K. Habibi; H. Rafati; A. Ahmadiani; A. Aliahmadi; M. Diederich
Abstract
Inflammation is one of the body’s defensive reactions against microorganisms and allergens. The NF-κB pathway is an essential component of the inflammatory responses involved in a number of respiratory tract diseases, such as asthma and chronic obstructive pulmonary disease. In traditional ...
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Inflammation is one of the body’s defensive reactions against microorganisms and allergens. The NF-κB pathway is an essential component of the inflammatory responses involved in a number of respiratory tract diseases, such as asthma and chronic obstructive pulmonary disease. In traditional medicine, Eucalyptus globulus Labill. (Myrtaceae) is used in inhalation therapy for respiratory tract infections and inflammation. In this study, the anti-inflammatory activity of Eucalyptus globulus essential oil and its impact on the NF-κB pathway was evaluated. A rat model of carrageenan-induced hind paw edema was used to evaluate the anti-inflammatory activity of E. globulus oil. Essential oil was injected intraperitoneally at doses of 0.5-3 mg/kg. An in vitro model based on a luciferase reporter gene construct (with repeats of a consensus NF-κB coding sequence) was used for investigation of the inhibitory effects of oil on the NF-κB pathway at concentrations of 100-250 μg/ml in Human chronic myeloid leukemia (K562) cells. E. globulus oil significantly reduced rat hind paw edema (P<0.05) at doses of 0.5 and 1.0 mg/kg at 1 to 3 h after carrageenan injection. The maximal reduction in edema was 58%. E. globules oil inhibited TNFα-induced NF-κB activity significantly (P<0.01) at a concentration of 250 μg/ml. The findings of the present study show that E. globulus essentialoil is a potent anti-inflammatory natural product, whose effects could be mediated through the NF-κB pathway.
M. Askary; M.A. Behdani; S. Parsa; M. Jamialahmadi; S. Mahmoodi
Abstract
This research was aimed to evaluate the effects of drought and manure on some physiological characteristics and yield of Thymus vulgaris L. and Thymus daenensis Celak.The study was conducted as factorial based on a randomized complete block design (RCBD) with three replications during crop years of 2015 ...
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This research was aimed to evaluate the effects of drought and manure on some physiological characteristics and yield of Thymus vulgaris L. and Thymus daenensis Celak.The study was conducted as factorial based on a randomized complete block design (RCBD) with three replications during crop years of 2015 and 2016 at the agriculture research farm of University of Birjand, Iran. Experimental factors were including: water stress at three levels (100%, 67% and 33% of field capacity), manure at two levels (30 tons.ha-1 and no manure), and two thyme species (Thymus vulgaris L. and Thymus daenensis Celak). Water stress reduced stomatal conductance, relative water content (RWC), Chl a, b and quantitative and qualitative yield; however, carotenoids content was increased under water stress. Although the quantity and quality yield of the study species decreased under water stress condition, manure application at 30 tons per ha led to the increased quantity and quality yield of both species. Thymus daenensis had more relative water content and quantity and quality yield (oil) in both crop years as compared with T. vulgaris. According to the results, T. daenensis can grow successfully in arid and semi-arid regions and could be recommended. There was a significant difference between two crop years; in the 2nd year, the quantity and quality yield (essential oil yield) of both study species increased.
