In collaboration with Scientific Association of Iranian Medicinal Plants

Document Type : Research Paper

Authors

1 Department of Palnt Production, Faculty of Agricultural Sciences and Natural Resources, Gonbad Kavoos University, Golestan, Iran

2 M.Sc. student, Agroecogology, Faculty of Agricultural Sciences and Natural Resources, Gonbad Kavoos University, Golestan, Iran

3 Ph.D. student Plant Physiology, Gonbad Kavoos University, Golestan, Iran

Abstract

In order to investigate the effects of different levels of vermicompost and nitroxin on the qualitative properties of medicinal plant marigold (Calendula officinalis L.), an experiment was conducted as factorial based on a completely randomized design with four replications in the greenhouse of Gonbad-e-Kavos University during 2014-2015. The first factor was vermicompost fertilizer treatment at six levels (0%, 10%, 20%, 40%, 70% and 100%) and the second factor was biological nitroxin fertilizer at two levels of inoculation (25 ml/kg soil) and no inoculation with nitroxin. The results of analysis of variance showed that the effects of nitroxin, vermicompost and their interaction on all traits studied were significant. However, the total phenol content was only significant in the vermicompost treatment. The results showed that the highest total phenol content, chlorophyll a, and total chlorophyll were obtained in 10% vermicompost treatment with nitroxin inoculation. The highest chlorophyll b content was related to 10% vermicompost treatment without nitroxin inoculation. The highest nitrogen content in the plant and catalase content were obtained from 100% vermicompost treatment with nitroxin inoculation, and 100% vermicompost without nitroxin inoculation, respectively. The highest amount of essential oil was obtained from 100% vermicompost treatment with nitroxin inoculation and the lowest was obtained from control treatment without nitroxin inoculation. According to the results, 100% and 70% vermicompost with nitroxin inoculation could be suggested as a suitable treatment for the production of marigold essential oil.

Keywords

- Allen, M.F., Moore, T.S. and Cheristenses, M., 1980. Phytohormone, changes in Bouteloua gracilis infected by vesicular-arbuscular mycorrhizae. I. Cytokine increase in the host plant. Canadian Journal of Botany, 58: 371-374.
- Amiri, M., Rezvani, M., Moghaddam, P. and Jahan, M., 2017. Effects of organic acids, mycorrhiza and rhizobacteria on yield and some phytochemical characteristics in low-input cropping system. Journal of Agricultural Knowledge and Sustainable Production, 27(1): 45-61.
- Arnon, D.I., 1949. Copper enzymes in isolated chloroplast. Polyphenoloxidase in Beta vulgaris. Plant Physiology, 24: 1-15.
- Beers, G.R. and Sizer, I.V., 1952. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. Journal of Biological Chemistry, 195: 133-140.
- Darzi, M.T. and Haj Seyed Hadi, M.R., 2012. Effects of the application of organic manure and biofertilizer on the fruit yield and yield components in Dill (Anethum graveolens). Journal of Medicinal Plants Research, 6(16): 3266-3271.
- Fallahi, J., Koocheki, A. and Rezvani Moghaddam, P., 2008. Investigating the effects of organic fertilizer on quantity index and the amount essential oil and chamazulene in chamomile (Matricaria recutita). Agricultural Research: Water, Soil and Plant in Agriculture, 1(8): 157-168.
- Kalvatchev, Z., Walder, R. and Garzaro, D., 1997. Anti-HIV activity of extracts from Calendula. Biomedicine & Pharmacotherapy, 51(4): 176-180.
- Khalesro, Sh., Ghalavand, A., Sefidkon, F. and Asgharzadeh, A., 2010. The effect of biological and organic inputs on quantity and quality of essential oil and some elements content of anise (Pimpinella anisum L.). Iranian Journal of Medicinal and Aromatic Plants, 27(4): 551-560.
- Khoramdel, S., Amin Ghafoori, A., Rezvani Moghaddam, P. and Nasiri Mahalati, M., 2011. Evaluation different irrigation regimes along with biofertilizer on grain yield, chlorophyll and relative water content of sesame. First Congress of Sustainable Agriculture and Healthy Food, Isfahan, 10-11 November.
- Kiani, Z., Esmaeilpour, B., Hadian, J., Soltani Toolarood, A.A. and Fathololumi, S., 2014. Effect of organic fertilizers on growth properties nutrient absorption and essential oil yield of medicinal plant of spearmint (Mentha spicata L.). Journal of Plant Production, 21(4): 63-80.
- Król, B., 2011. Yield and the chemical composition of flower head of Pot marigold (Calendula officinalis L.) depending on nitrogen fertilization. ACTA Scientiarum Polonorum-Hortorum Cultus, 10(2): 235-243.
- Lin, T., Zhu, X. and Zhang, F., 2012. The interaction effect of cadmium and nitrogen on Populus yunnanensis. Journal of Agricultural Science, 4(2): 125-134.
- Mahfouz, S.A. and Sharaf Eldin M.A., 2007. Effect of mineral vs. biofertilizer on growth, yield, and essential oil content of fennel (Foeniculum vulgare Mill.). International Agrophysics, 21(4): 361-366.
- Makkizadeh, M., Nasrollahzadeh, S., Zehtab Salmasi, S., Chaichi, M. and Khavazi, K., 2012. The effect of organic, biologic and chemical fertilizers on quantitative and qualitative characteristics of sweet basil (Ocimum basilicum L.). Journal of Agriculture Science and Sustainable Production, 22(1): 1-12.
- Malik, C.P. and Singh, M.B., 1980. Plant Enzymology and Histo-Enzymologhy. Kalyani Publishers. New Dehli, 434p.
- Mardani, F. and Amooaghaie, R., 2016. Effect of vermicompost and its extract on emergence and the growth parameters of (Plantago psyllium). Journal of Science and Technology of Greenhouse Culture Soilless Culture Research Center Ejgcst, 7(1): 1-13.
- Marius, S., Octavita, A., Eugen, U. and Vlad, A., 2005. Study of a microbial inoculation on several biochemical indices in sun flower (Helianthus anuus L.). Analele tiinifice aleUniversitii Alexandru Ioan Cuza Genetici Biologie Molecular, TOM V, 234: 215-273.
- Mazandarani, M., Zarghami Moghadam., Zolfaghari, M.R. and Ghaemi, E., 2012. Effect of solvent type on TP and TF content and antioxidant activity in Onosma dichroanthum Boiss. in Golestan province, North of Iran. Journal of Medicinal plant Research, 6(28): 4481-4488.
- Meda, A., Lamien, C.E., Romito, M., Millogo, J. and Nacoulma, G.O., 2005. Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity. Food Chemistry, 91(3): 571-577.
- Moradi, R., Rezvani, M.P., Nasiri, M.M. and Lakzian, A., 2010. The effect of application of organic and biological fertilizers on yield, yield components and essential oil of Foeniculum vulgare (Fennel). Iranian Journal of Agronomy Research, 7(2): 625-635.
- Patric, K. and Kumar, S., 2005. Calendula. EBSCO Publishin, 14: 100-171.
- Peñuelas, J., Gamon, J., Freeden, A., Merino, J. and Field, C., 1994. Reflectance indices associated with physiological changes in N and water-limited sunflower leaves. Remote Sensing and Environment, 48(2): 135-146.
- Radzi, M.N., Mahadzir, N. and Rashid, F.N., 2017. Effect of different rate of vermicompost on growth performance and yield of rice (Oryza sativa). International Journal of Engineering Sciences & Management Research, 4(3): 47-49.
- Rahimi, A.R., Mashayekhi, K., Amini, S. and Soltani, E., 2009. Effect of mineral vs. biofertilizer on the growth, yield and essential oil content of Coriander (Coriandrum sativum L.). Medicinal and Aromatic Plant Science and Biotechnology, 3(2): 82-84.
- Rahimzadeh, S., Sohrabi, Y., Heidari, G.R., Eivazi, A. and Hoseini, S.M., 2013. Effect of biofertilizers on macro and micro nutrients uptake and essential oil content in (Dracocephalum moldavica L.). Iranian Journal of Field Crops Research, 11: 179-190.
- Rahimzadeh, S., Sohrabi, Y., Heidary, G.R. and Pirzad, A.R., 2011. Effect of biofertilizers application on some morphological traits and yield of dragonhead (Dracocephalum moldavica L.). Journal of Horticultural Science, 25(3): 335-343.
- Rezaei, M. and Baradaran, R., 2013. Effects of biofertilizers on the yield and yield components of pot marigold (Calendula officinalis L.). Iranian Journal of Medicinal and Aromatic Plants, 29(3): 635-650.
- Saikia, L.R. and Upadhyaya, S., 2011. Antioxidant activity, phenol and flavonoid content of A. racemosus Willd. A medicinal plant grown using different organic manures. Research Journal of Pharmaceutical, Biological & Chemical Sciences, 2(2): 457-463.
- Sajadi Nik, R. and Yadavi, A.R., 2013. Effect of nitrogen fertilizer, vermicompost and nitroxin on growth indexes, phonological stages and grain yield of Sesame. Electronic Journal of crop prodution, 6(2): 73-99.
- Salehi, A., Ghalavand, A., Sefidkon, F. and. Asgharzade, A., 2010. The effect of zeolite, PGPR and vermicompost application on N, P, K concentration, essential oil content and yield in organic cultivation of German chamomile (Matricaria chamomilla L.). Iranian Journal of Medicinal and Aromatic Plants, 27(2): 188-201.
- Shahhoseini, R., Omidbaigi, R. and Kiani, D., 2012. Effect of biological fertilizers of biosulfur, nitroxin and super absorbent polymer on growth, yield and essential oil content of sweet basil (Ocimum basilicum L.). Journal of Horticulture Science, 26: 246-254.
- Shokrani, F., Pirzad, A., Zardoshti, M.R. and Darvishzadeh, R., 2012. Effect of irrigation disruption and biological nitrogen on growth and flower yield in (Calendula officinalis L.). African Journal of Biotechnol, 11(21): 4795-4802.
- Sindhu, G., Raborthy, C. and Prashant, M., 2010. Determination of quercetin HPTLC in Calendula officinalis extract. International Journal of Pharma and Biology Science, 1(1): 1-4.
- Vessey, H., Liaghati, H. and Alipour, A., 2016. Developing an ethics-based approach to indicators of sustainable agriculture using analytic hierarchy process (AHP). Ecological Indicators, 60: 644-654.
- Vejan, P., Abdullah, R., Khadiran, T., Salmah, I. and Nasrulhaq Boyce, A., 2016. Role of Plant Growth Promoting Rhizobacteria in Agricultural Sustainability-A Review. Molecules, 21(5): 2-17.
- Wu, S.C., Cao, Z.H., Li, Z.G., Cheung, K.C. and Wong, M.H., 2005. Effects of biofertilizers containing N-fixer, P and K solubilizer and AM fungi on maize growth: a greenhouse trail. Geoderma, 125: 155-166.