Anti-inflammatory activity of essential oil derived from Eucalyptus globulus Labill. and its impact on NF-B pathway

Habibi, K.; Rafati, H.; Ahmadiani, A.; Aliahmadi, A.; Diederich, M.

doi:10.22092/ijmapr.2017.107950.1863

Document Type : Research Paper

Authors

¹ Ph.D. Student, Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran

² Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran

³ Department of Pharmacology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

⁴ Department of Biology, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran

⁵ Department of Pharmacy, College of Pharmacy, Seoul National University, Seoul, Korea

https://doi.org/10.22092/ijmapr.2017.107950.1863

Abstract

Inflammation is one of the body’s defensive reactions against microorganisms and allergens. The NF-κB pathway is an essential component of the inflammatory responses involved in a number of respiratory tract diseases, such as asthma and chronic obstructive pulmonary disease. In traditional medicine, Eucalyptus globulus Labill. (Myrtaceae) is used in inhalation therapy for respiratory tract infections and inflammation. In this study, the anti-inflammatory activity of Eucalyptus globulus essential oil and its impact on the NF-κB pathway was evaluated. A rat model of carrageenan-induced hind paw edema was used to evaluate the anti-inflammatory activity of E. globulus oil. Essential oil was injected intraperitoneally at doses of 0.5-3 mg/kg. An in vitro model based on a luciferase reporter gene construct (with repeats of a consensus NF-κB coding sequence) was used for investigation of the inhibitory effects of oil on the NF-κB pathway at concentrations of 100-250 μg/ml in Human chronic myeloid leukemia (K562) cells. E. globulus oil significantly reduced rat hind paw edema (P<0.05) at doses of 0.5 and 1.0 mg/kg at 1 to 3 h after carrageenan injection. The maximal reduction in edema was 58%. E. globules oil inhibited TNFα-induced NF-κB activity significantly (P<0.01) at a concentration of 250 μg/ml. The findings of the present study show that E. globulus essentialoil is a potent anti-inflammatory natural product, whose effects could be mediated through the NF-κB pathway.

Keywords

References

- Almeida, I.F., Fernandes, E., Lima, J.L., Valentão, P., Andrade, P.B., Seabra, R.M., Costa, P.C. and Bahia, M.F., 2009. Oxygen and nitrogen reactive species are effectively scavenged by Eucalyptus globulus leaf water extract. Journal of Medicinal Food, 12(1): 175-183.

- Duvoix, A., Delhalle, S., Blasius, R., Schnekenburger, M., Morceau, F., Fougère, M., Henry, E., Galteau, M.M., Dicato, M. and Diederich, M., 2004. Effect of chemopreventive agents on glutathione S-transferase P1-1 gene expression mechanisms via activating protein 1 and nuclear factor kappa B inhibition. Biochemical Pharmacology, 68(6): 1101-1111.

- Edwards, M.R., Bartlett, N.W., Clarke, D., Birrell, M., Belvisi, M. and Johnston, S.L., 2009. Targeting the NF-kappaB pathway in asthma and chronic obstructive pulmonary disease. Pharmacology and Therapeutics, 121(1): 1-13.

- Fereidoni, M., Ahmadiani, A., Semnanian, S. and Javan, M., 2000. An accurate and simple method for measurement of paw edema. Journal of Pharmacological and Toxicological Methods, 43(1): 11-14.

- Garg A. and Aggarwal B.B., 2002. Nuclear transcription factor-kappaB as a target for cancer drug development. Leukemia, 16(6): 1053-1068.

- Giamperi, L., Fraternale, D. and Ricci, D., 2002. The in vitro action of essential oils on different organisms. Journal of Essential Oil Research, 14(4): 312-318.

- Gilmore, T.D., 2006. Introduction to NF-kappaB: players, pathways, perspectives. Oncogene, 25(51): 6680-6684.

- Greiner, J.F.W., Müller, J., Zeuner, M.T., Hauser, S., Seidel, T., Klenke, C.H., Grunwald, L.M., Schomann, T., Widera, D., Sudhoff, H., Kaltschmidt, B. and Kaltschmidt, C.H., 2013. 1,8-Cineol inhibits nuclear translocation of NF-κB p65 and NF-κB dependent transcriptional activity. Biochimica et Biophysica Acta, 1833(12): 2866-2878.

- Juergens, U.R., Stöber, M., Schmidt-Schilling, L., Kleuver, T. and Vetter, H., 1998. Anti-inflammatory effects of 1,8-cineole in bronchial asthma: inhibition of arachidonic acid metabolism in human blood monocytes ex vivo. European Journal of Medical Research, 3(9): 407-412.

- Juergens, U.R., Engelen, T., Racké, K., Stöber, M., Gillissen, A. and Vetter, H., 2004. Inhibitory activity of 1,8-cineol (eucalyptol) on cytokine production in cultured human lymphocytes and monocytes. Pulmonary Pharmacology and Therapeutics, 17(5): 281-287.

- Kumar, P., Mishra, S., Malik, A. and Satya, S., 2012. Compositional analysis and insecticidal activity of Eucalyptus globulus (family: Myrtaceae) essential oil against housefly (Musca domestica). Acta Tropica, 122(2): 212-218.

- Liapi, Ch., Anifantis, G., Chinou, I., Kourounakis, A., Theodosopoulos, S. and Galanopoulos, P., 2007. Antinociceptive properties of 1,8-cineol and beta-pinene, from the essential oil of Eucalyptus camaldulensis leaves, in Rodentsrodents. Planta Medica, 73(12): 1254-1274.

- Lima, D.F., Brandão, M.S., Moura, J.B., Leitão, J.M., Carvalho, F.A., Miúra, L.M., Leite, J.R., Sousa, D.P. and Almeida, F.R., 2012. Antinociceptive activity of the monoterpene α-phellandrene in rodents: Possible mechanisms of action. Journal of Pharmacology & Pharmacotherapeutics, 64(2): 283-292.

- Lima, P.R., Melo, T.S., Carvalho, K.M., de Oliveira, I.B., Arruda, B.R., de Castro Brito, G.A., Rao, V.S. and Santos, F.A., 2013. 1,8-cineole (eucalyptol) ameliorates cerulean induced acute pancreatitis via modulation of cytokines, oxidative stress and NF-ΚB activity in mice. Life Sciences, 92(24-26): 1195-1201.

- Moore, M.M, Chua, W., Charles, K.A. and Clarke, S.J., 2010. Inflammation and cancer: causes and consequences. Clinical Pharmacology & Therapeutics, 87(4): 504-508.

- Papachristos, D.P., Karamanoli, K.I., Stamopoulos, D.C. and Menkissoglu-Spiroudi, U., 2004. The relationship between the chemical composition of three essential oils and their insecticidal activity against Acanthoscelides obtectus. Pest Management Science, 60(5): 514-522.

- Paur, I., Balstad, T.R., Kolberg, M., Pedersen, M.K., Austenaa, L.M., Jacobs, D.R. and Blomhoff, R., 2010. Extract of oregano, coffee, thyme, clove, and walnuts inhibits NF-kappaB in monocytes and in transgenic reporter mice. Cancer Prevention Research, 3(5): 653-663.

- Reichling, J., Schnitzler, P., Suschke, U. and Saller, R., 2009. Essential oils of aromatic plants with antibacterial, antifungal, antiviral, and cytotoxic propertiesan overview. Forschende Komplementärmedizin, 16(2): 79-90.

- Santos, F.A. and Rao, V.S.N., 2000. Anti-inflammatory and anti-nociceptive effects of 1,8-cineole a terpenoid oxide present in many plant essential oils. Phytotherapy Research, 14(4): 240-244.

- Serafino, A., Vallebona, P.S., Andreola, F., Zonfrillo, M., Mercuri, L., Federici, M., Rasi, G., Garaci, E. and Pierimarchi, P., 2008. Stimulatory effect of Eucalyptus essential oil on innate cell-mediated immune response. BMC Immunology, 9: 17-33.

- Silva, J., Abebeb, W., Sousa, S.M., Duarte, V.G., Machado, M.I.L. and Matos, F.J.A., 2003. Analgesic and anti-inflammatory effects of essential oils of Eucalyptus. Journal of Ethnopharmacology, 89(2-3): 277-283.

- Siqueira, H., Neto, B., Sousa, D., Gomes, B., Cunha, F. and Oliveira, F., 2016. Antiedematogenic Effect of α-Phellandrene in Animal Models. Current Bioactive Compounds, 12(2): 107-113.

- Taubes, G., 2002. Does inflammation cut to the heart of the matter?. Science, 296(5566): 242-245.

- Vigo, E., Cepeda, A., Gualillo, O. and Perez-Fernandez, R., 2004. In-vitro anti-inflammatory effect of Eucalyptus globulus and Thymus vulgaris: nitric Nitric oxide inhibition in J774A.1 murine macrophages. Journal of Pharmacology & Pharmacotherapeutics, 56(2): 257-263.

- White, P., 2005. COPD: Challenges and opportunities for primary Care. Respiratory Medicine: COPD Update, 1(2): 43-52.

- Winter, C., 1962. Carrageenan-induced edema in hind paws of the rat as an assay for anti-inflammatorry drugs. Proceedings of the Society for Experimental Biology and Medicine, 111: 544-547.

- Wong, E.T. and Tergaonkar, V., 2009. Roles of NF-ΚB in health and disease: mechanisms Mechanisms and therapeutic potential. Clinical Science, 116(6): 451-465.

- Zargari, A., 1991. Medicinal Plants. (Vol. 2), Tehran University Press, Tehran, 942p.

Iranian Journal of Medicinal and Aromatic Plants Research

Anti-inflammatory activity of essential oil derived from Eucalyptus globulus Labill. and its impact on NF-B pathway

References

References

Volume 33, Issue 5 - Serial Number 85
December 2017
Pages 769-778

Anti-inflammatory activity of essential oil derived from Eucalyptus globulus Labill. and its impact on NF-B pathway

References

References

Volume 33, Issue 5 - Serial Number 85December 2017Pages 769-778

Volume 33, Issue 5 - Serial Number 85
December 2017
Pages 769-778