S. Hossein Jafari; A. Saadatfar
Abstract
Black caraway, Bunium persicum (Boiss.) B. Fedtsch., belonging to the Apiaceae family, is one of the most important medicinal plants in Iran. In this study, genetic and phytochemical relationships were investigated among some populations of this plant using ISSR markers and GC-MS method in Yazd and Kerman ...
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Black caraway, Bunium persicum (Boiss.) B. Fedtsch., belonging to the Apiaceae family, is one of the most important medicinal plants in Iran. In this study, genetic and phytochemical relationships were investigated among some populations of this plant using ISSR markers and GC-MS method in Yazd and Kerman habitats. In order to study genetics using 10 ISSR primers, sampling of the fresh plant leaves was done. DNA was extracted using CTAB method. To investigate phytochemicals, the essential oil was extracted from black caraway seeds using hydrodistillation method and Clevenger apparatus. According to the results, ISSR-13 primer had better performance with 40 bands, the highest amounts of PIC (0.39), and Marker Index (15.6). Cluster analysis using Jacquard's coefficient and UPGMA algorithm divided the four black caraway populations of the two provinces into six groups. Principal coordinates analysis showed that three components explained 60.56% of total variance and completely separated the populations. The results of this analysis were consistent with cluster analysis and geographical distribution of samples. Based on the GC-MS results, 13 and 16 compounds were identified in Yazd and Kerman habitats, respectively. The compounds α-pinene, β-pinene, ρ-cymene, limonene, γ-terpinene, and cuminaldehyde were identified as the same and main compounds between Yazd and Kerman habitats. Based on the results of cluster analysis of phytochemical parameters and location of the habitats Sirjan (Kerman province), Mehriz and Ardekan (Yazd province) in one cluster and Sirch in a separate cluster, it can be concluded that grouping based on essential oil compounds does not correspond with genetic grouping and geographical distance in the natural habitats.
F. Oroojalian; R. Kasra-Kermanshahi; M. Azizi; M.R. Bassami
Abstract
Essential oils of three medicinal plants species, including Carum copticum (L.) C. B. Clarke, Bunium persicum (Boiss.) B. Fedtsch. and Cuminum cyminum L., were obtained by hydrodistillation and their constituents were analyzed by GC and GC/MS using retention indices and fragmentation patterns. The antibacterial ...
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Essential oils of three medicinal plants species, including Carum copticum (L.) C. B. Clarke, Bunium persicum (Boiss.) B. Fedtsch. and Cuminum cyminum L., were obtained by hydrodistillation and their constituents were analyzed by GC and GC/MS using retention indices and fragmentation patterns. The antibacterial effects (MIC and MBC) of the essential oils were assessed on several food-borne pathogens, namely Staphylococcus aureus, Bacillus cereus, Escherichia coli O157: H7, Salmonella enteritidis and Listeria monocytogenes by microdilution technique using ELISA reader. Because of the combinatory usage of Bunium persicum and Cuminum cyminum in folk medicine, the affect of essential oil on food organoleptic properties and MIC values of the plants, interaction of Bunium persicum and Cuminum cyminum essential oils were also studied by FICindex determination using modified dilution checkboard method. The results showed that there are noticeable differences between the essential oils as their constituents as concerned, while ρ-cymen and γ-terpinene detected in all essential oils in different percentage. The main components of essential oils of C. copticum were thymol (48.4%), ρ-cymene(21.8%) and γ-terpinene (21.3%). The major constituents of B. persicum were γ-terpinene (44.2%), cuminaldehyde (16.9%), γ-terpinen-7-al (10.5%), and ρ-cymen (8%) while those of C. cyminum were cuminaldehyde (30.2%), ρ-cymene(14.1%), γ-terpinene (12.8%), and safranal (9.4%). The ranges of minimum inhibitory concentrationof the oils were 0.03-0.5,0.18-3.0, and 0.37-3.0 mg/ml, respectively, for C. copticum, B. persicum and C. cyminum. Moreover, the combination of B. persicum and C. cyminum essential oils confirmed synergistic and additive activities against the pathogens. In conclusion, although the MIC of Bunium persicum and Cuminum cyminum essential oils were lower than C. copticum, but combinatory usage of these essential oils especially against gram-positive bacteria produced promissing results. So application of these essential oils is recommended in combination as an efficients and complementary method for control of food borne pathogens with lower side effects on organolpetic properties of food.
S. Bahador; A. Negari; M. Abbaspoor
Abstract
Bunium persicum (Boiss.) B. Fedtsch. is a perennial and aromatic plant from Apiaceae family that distributes in most Iranian habitats. It is used in medicinal, food, cosmetic-hygienic and drinks industries, moreover it is used in traditional medicine. The investigations show, there are suitable conditions ...
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Bunium persicum (Boiss.) B. Fedtsch. is a perennial and aromatic plant from Apiaceae family that distributes in most Iranian habitats. It is used in medicinal, food, cosmetic-hygienic and drinks industries, moreover it is used in traditional medicine. The investigations show, there are suitable conditions to produce this precious plant in Iran and for a better production some studies needs to be done. In order to investigate the effect of planting depth and corm weight on agronomical characteristics Bunium persicum, an experiment was conducted based on factorial design in randomized complete block with three replications. Depths of planting were in 5 levels (5, 10, 15, 20, 25cm) and the corm weights were in 3 levels (≤2, 2-4, 4-6gr) in 2007 and the design was conducted in research farm of training organization of agriculture of Khorasan. The results showed, the effect of depth of planting on the emergence percent, height, the number of umbrella, the yield and the weight of thousand seeds in plant were very significant (≤α99%). The effect of corm weights was significant only on high plants. The interaction of two factors was significant in the high, the number of umbrella and yield characteristics. The most seed yield was related to 10 cm deep and the lowest to 25 cm depth. The highest weight of 1000 seeds and the lowest were related also to these levels (10 and 25 cm). As a result it is recommended in planting the corms of Bunium persicum the depth of planting must be about 10cm and the weight corm must be more than 2 gram.
S. Dehghan Kouhestani; A. Baghizadeh; Gh.A. Ranjbar; N.A. Babaiyan Jelodar
Volume 24, Issue 4 , February 2009, , Pages 414-427
Abstract
Persian Cumin, a medicinal and aromatic plant, belongs to Apiaceae family. The objective of this study was to assess the genetic variation in germplasm of Persian Cumin collected from Kerman province using RAPD molecular markers. The Persian Cumin fruits were collected from 43 various regions in Kerman ...
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Persian Cumin, a medicinal and aromatic plant, belongs to Apiaceae family. The objective of this study was to assess the genetic variation in germplasm of Persian Cumin collected from Kerman province using RAPD molecular markers. The Persian Cumin fruits were collected from 43 various regions in Kerman province. DNA was extracted from fruits using modified CTAB method. From the 27 used primers in PCR, 19 primers with better bands were selected for analysis. The 446 polymorphic bands obtained by these primers were scored. The binary matrix was converted to distance matrix by applying Dice similarity coefficient in NTSYS-pc (Ver 2.02) software. Then, the distance matrix was analyzed using UPGMA and the phyllogenic dendrogram was plotted. Based on the results, investigated populations were clustered in 7 groups. The obtained clusters based on RAPD markers to some extent matched with the geographical origin of the studied populations of Persian Cumin. Furthermore, the obtained results of principal component analysis method were similar to the results of cluster analysis. The results indicated that RAPD technique is an efficient tool for assessing genetic diversity in these populations.
