Iranian Journal of Medicinal and Aromatic Plants Research

In collaboration with Scientific Association of Iranian Medicinal Plants

Current Issue

By Issue

By Author

By Subject

Author Index

Keyword Index

About Journal

Aims and Scope

Publication Ethics

Indexing and Abstracting

Related Links

FAQ

Peer Review Process

Journal Metrics

News

Synergistic antibacterial activity of the essential oils from three medicinal plants against some important food-borne pathogens by microdilution method

Document Type : Research Paper

Authors

1 MSc Student, Microbiology Division, Biology Department, Faculty of Science, Isfahan University, Isfahan, Iran

2 Microbiology Division, Biology Department, Faculty of Science, Alzahra University, Tehran, Iran

3 Department of Horticulture, College of Agriculture, Ferdowsi University of Mashhad, Iran

4 Biotechnology Division, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Iran

Abstract

Essential oils of three medicinal plants species, including Carum copticum (L.) C. B. Clarke, Bunium persicum (Boiss.) B. Fedtsch. and Cuminum cyminum L., were obtained by hydrodistillation and their constituents were analyzed by GC and GC/MS using retention indices and fragmentation patterns. The antibacterial effects (MIC and MBC) of the essential oils were assessed on several food-borne pathogens, namely Staphylococcus aureus, Bacillus cereus, Escherichia coli O157: H7, Salmonella enteritidis and Listeria monocytogenes by microdilution technique using ELISA reader. Because of the combinatory usage of Bunium persicum and Cuminum cyminum in folk medicine, the affect of essential oil on food organoleptic properties and MIC values of the plants, interaction of Bunium persicum and Cuminum cyminum essential oils were also studied by FICindex determination using modified dilution checkboard method. The results showed that there are noticeable differences between the essential oils as their constituents as concerned, while ρ-cymen and γ-terpinene detected in all essential oils in different percentage. The main components of essential oils of C. copticum were thymol (48.4%), ρ-cymene(21.8%) and γ-terpinene (21.3%). The major constituents of B. persicum were γ-terpinene (44.2%), cuminaldehyde (16.9%), γ-terpinen-7-al (10.5%), and ρ-cymen (8%) while those of C. cyminum were cuminaldehyde (30.2%), ρ-cymene(14.1%), γ-terpinene (12.8%), and safranal (9.4%). The ranges of minimum inhibitory concentrationof the oils were 0.03-0.5,0.18-3.0, and 0.37-3.0 mg/ml, respectively, for C. copticum, B. persicum and C. cyminum. Moreover, the combination of B. persicum and C. cyminum essential oils confirmed synergistic and additive activities against the pathogens. In conclusion, although the MIC of Bunium persicum and Cuminum cyminum essential oils were lower than C. copticum, but combinatory usage of these essential oils especially against gram-positive bacteria produced promissing results. So application of  these essential oils is recommended in combination as an efficients and complementary method for control of food borne pathogens with lower side effects on organolpetic properties of food.

Keywords

References
- زرگری، ع.، 1368. گیاهان دارویی. جلد چهارم، انتشارات دانشگاه تهران، 130 صفحه.
- کرمانشاهی، ر.، معطر، ف. و سلیمانی منش، ع.، 1385. ارزیابی اثرهای ضد باکتریایی عصاره‌های آبی و الکلی گیاه گلرنگ بر روی تعدادی از باکتریها. مجله علوم دانشگاه شهید چمران، 15: 25-18.
- مقتدر، م.، ایرج منصوری، ع.، سالاری، ح. و فرهمند، آ.، 1388. شناسایی ترکیبات شیمیایی و بررسی اثرهای ضد میکروبی اسانس بذر زیره سیاه. تحقیقات گیاهان دارویی و معطر، 25(1): 28-20.
- Adams, R.P., 2001. Identification of essential oil components by Gas Chromatography and Mass Spectrometry. Allured, USA, 750p.
- Atal, C.K. and Kapur, B.M., 1982. Cultivation and Utilization of Aromatic Plants.Regional Research Laboratory, Jammu-Tawi, 815p.
- Atta-ur-Rahman, M.I., Choudhary, I., Farooq, A., Ahmed, A., Iqbal, M.Z., Demirci, B. Demirci, F. and Baser, K.H.C., 1999. Antifungal activity and essential oils constituents of some spices from Pakistan. Third International Electronic Conference on Synthetic Organic Chemistry, www/reprint.net/ecsco-3.htm.
- Azizi, M., 2005. Bunium persicum essential oils suppressed potato sprouting in storage. Proceedings of the 36th International Symposium on Essential oils, Budapest, Hungary, 4-7 September: 142.
- Azizi, M., Davarenejad, Gh., Bos, R., Woerdenbag, H.J. and Kayser, O., 2009.Essential oil content and constituents of Black Zira (Bunium persicum [Boiss.] B. Fedtsch.) from Iran during field cultivation (domestication). Journal of Essential Oil Research, 21: 78-82.
- Bandoni, A., Juarez, M.A. and Mizrahi, I., 1991. Studies on the essential oil of cumin (Cuminum cyminum L.). Essenze e Derivati Agrumari, 61: 32-49.
- Baser, K.H.C., Özek, T., Abduganiev, B.E., Abdullaev, U.A. and Aripov, K.N., 1997. Composition of the essential oil of Bunium persicum (Boiss.) B. Fedtsch. from Tajikistan. Journal of Essential Oil Research, 9: 597-598.
- Beis, SH., Azcan, N., Ozek, T., Kara, M. and Baser, K.H.C., 2000. Production of essential oil from cumin seeds. Chemistry of Natural Compounds, 36: 265-268.
- Boskabady, M.H., Ramazani, M. and Tabei, T., 2003. Relaxant effects of different fractions of essential oil from Carum copticum on guinea pig tracheal chains. Phytotherapy Research, 17: 1145-1149.
- Boskabady, M.H. and Shaikhi, J., 2000. Inhibitory effect of Carum copticum on histamine (H1) receptors of isolated guinea-pig tracheal chains. Journal of Ethnopharmacology, 69: 217-227.
- British pharmacopoeia., 1988. Vol. 2, London: HMSO, pp. 137-138.
- Burt, S., 2004. Essential oils: Their antibacterial properties and potential applications in foods- a review. International Journal of Food Microbiology, 94: 223-253.
- Celiktas, O.Y., Hames Kocabas, E.E., Bedir, E., Vardar Sukan, F., Ozek, T. and Baser, K.H.C., 2007. Antimicrobial activities of methanol extracts and essential oils of Rosmarinus officinalis, depending on location and seasonal variations. Food Chemistry, 100: 553-559.
- Daferera, D.J., Ziogas, B.N. and Polissiou, M.G., 2000. GC/MS analysis of essential oils from some Greek aromatic plants and their fungitoxicity on Penicillium digitatum. Journal of Agricultural Food Chemistry, 48: 2576-2581.
- Demirci, F., Guven, K., Demirci, B., Dadandi, M.Y. and Baser, K.H.C., 2008. Antibacterial activity of two Phlomis essential oils against food pathogens. Food Control, 19: 1159-1164.
- D’Mello, J.P.F., 2003. Food Safety: Contaminants and Toxins. Oxford, Cabi publisher, 476p.
- Eikani, M.H., Goodarznia, I. and Mirza, M., 1999. Supercritical carbon dioxide extraction of cumin seeds (Cuminum cyminum L.). Flavour and Fragrance Journal, 14: 29-31.
- El-Wakeil, F., Morsi, M. and Khairy, S., 1986. Effects of various storage conditions on the quality of some spice essential oils. Seifen Oele Fette Waches; 112: 350-353.
- Friedman, M., Henika, P.R. and Mandrell, R.E., 2002. Bactericidal activities of plant essential oils and some of their isolated constituents against Campylobacter jejuni, Escherichia coli, Listeria monocytogenese and Salmonella enterica. Journal of Food Protection, 65(10): 1545-1560.
- Gachkar, L., Yadegari, D., Rezaei, M.B., Taghizadeh, M., Alipoor Astaneh, Sh. and Rasooli, I., 2007. Chemical and biological characteristics of Cuminum cyminum and Rosmarinus officinalis essential oils. Food Chemistry, 102: 898-904.
- Gonzalez, J.A., Estevez-Braun, A., Estevez-Reyes, R., Bazzocchi, I.L., Moujir, L., Jimenez, I. A., Ravelo, A.G. and Gonzalez, A.G., 1995. Biological activity of secondary metabolites from Bupleurum salicifolium (Umbelliferae). Experientia, 51: 35-39.
- Holly, R.A. and Patal, D., 2005. Improvement in shelf life and safetey of perishable foods by plant essential oils and smoke antimicrobials, Food Chemistry, 22: 273-292.
- Iacobellis, N.S., Cantore, P.L. Capasso, F. and Senatore, F., 2005. Antibacterial activity of Cuminum cyminum and Carun carvi essential oils. Journal of Agricultural and Food Chemistry, 53: 57-61.
- Kalemba, D. and Kunicka, A., 2003. Antibacterial and antifungal properties of essential oils. Current Medicinal Chemistry, 10: 813-829.
- Karim, A., Pervez, M. and Bhatty, M.K., 1997. Studies on the essential oils of the Pakistani species of the familie Umbelliferae, part 10. Bunium persicum Boiss. (Sah Zira) seed oil. Pakistan Journal of Scientific and Industrial Research, 20(2): 106-108.
- Khajeh, M., Yamini, Y., Sefidkon, F. and Bahramifar, N., 2004. Comparison of essential oil composition of Carum copticum obtained by supercritical carbon dioxide extraction and hydrodistillation methods. Food Chemistry, 86: 587-591.
- Kordali, S., Kotan, R., Mavi, A., Cakir, A., Ala, A. and Yildirim, A., 2005. Determination of the chemical composition and antioxidant activity of the essential oil of Artemisia dracunculus and of the antifungal and antibacterial activities of Turkish Artemisia absinthium, A. dracunculus, Artemisia santonicum and Artemisia spicigera essential oils. Journal of Agricultural and Food Chemistry, 53: 9452-9458.
- Kotzé, M. and Eloff, J.N., 2002. Extraction of antibacterial compound from Combretum microphyllum (Combretaceae). South African Journal of Botany, 68: 62-67.
- Kumar, P. and Baslas, R.K., 1978. Chemical examination of essential oil of the seeds of Cuminum cyminum. Indian Perfumer, 22: 164-165.
- Moghadamzadeh, A., Faridi, P. and Ghasemi, Y., 2007. Carum copticum Benth & Hook. essential oil chemotypes. Food Chemistry, 100: 1217-1219.
- Pae, H.O., Oh, H., Yun, Y.G., Oh, G.S., Jang, S.I., Hwang, K.M., Kwon, T.O., Lee, H.S. and Chung, H.T., 2002. Imperatorin, a furanocoumarin from Angelica dahurica (Umbelliferae), induces cytochrome c-dependent apoptosis in human promyelocytic leukaemia, HL-60 cells. Pharmacololgy Toxicology. 91: 40-48.
- Pillai, S.K., Moellering, C. and Elipoulos, G.M., 2005. Antimicrobial combinations. 365-373, In: V. Lorian, (ed.), Antibiotic in laboratory medicine, Lippincott Williams & Wilkins, Philadelphia, 922p.
- Pourmortazavi, S.M., Ghandiri, M. and Hajimirsadeghi, S.S., 2005. Supercritical fluid extraction of volatile components from Bunium persicum Boiss. (Black cumin) and Mespilus germanica L. (medlar) seeds. Journal of Food Componds Analytica, 18: 439-446
- Qadry, S.M.J.S. and Atal, C.K., 1976. Pharamacognosy of the fruits of Trachyspermum ammi. Planta Medica, 15: 53-57.
- Rani, P. and Khullar, N., 2004. Antimicrobial evaluation of some medicinal plants for their anti-enteric potential against Multi-drug resistant Salmonella typhi. Phytotheraphy Research, 18: 670-673.
- Rechinger, K.H., 1965-2001. Flora Iranica, Akademische Druch-u, Verlagsanstat, Graz-Austria.
- Sadykov, Y.D., Kurbanov, M., Khavizov, K. and Regovatov, Y.M., 1978. Composition of the essential oil from fruits of Bunium persicum (Boiss.) B. Fedtsh. Doklady Akademii Nauk Tadzhikskoj SSR., 21(5): 33-36.
- Sandri, I.G., Zacaria, J., Fracaro, F., Delamare, A.P.L. and Echeverrigaray, S., 2007. Antimicrobial activity of the essential oils of Brazilian species of the genus Culina against foodborne pathogens and spoiling bacteria. Food Chemistry, 103: 823-828.
- Santiesteban-López, A., Palou, E. and López-Malo, A., 2007. Susceptibility of food-borne bacteria to binary combinations of antimicrobials at selected aw and pH. Journal of Applied Microbiology,102: 486-497.
- Singh, G., Kapoor, I.P.S., Pandey, S.K., Singh, U.K. and Singh, R.K., 2002. Studies on essential oils: Part 10; Antibacterial activity of volatile oils from some species. Phytotherapy Research, 16: 680-682.
- Srivastava, M., Saxena, A. and Baby, P., 1999. GC/MS investigation and antimicrobial activity of the essential oil of Carum copticum Benth & Hook. Acta Alimentaria, 28: 291-295.
- Tepe, B., Donmez, E., Unlu, M., Candan, F., Daferera, D. and Vardar-Unlu, G., 2004. Antimicrobial and antioxidative activities of the essential oils and methanol extracts of Salvia cryptantha (montbret et aucher ex benth.) and Salvia multicaulis (vahl). Food Chemistry, 84: 519-525.
- Ulte, A., Bennik, M.H.J. and Moezelaar, R., 2002. The phenolic hydroxyl group of carvacrol is essential for action against the food pathogen Bacillus cereus. Applied and Environmental Microbiology, 68: 1561-1568.
- Ye, Y.N., Liu, E.S.L., Li, Y., So, H.L., Cho, C.C.M., Sheng, H.P., Lee, S.S. and Cho, C.H., 2001. Protective effect of polysaccharides-enriched fraction from Angelica sinensis on hepatic injury. Life Science, 69: 637-646.
Iranian Journal of Medicinal and Aromatic Plants Research
Volume 26, Issue 2 - Serial Number 48
August 2010
Pages 133-146
Files
Share
How to cite
Statistics