Improvement and breeding
M. Salehi Vozhdehnazari; Z. Shirazi; S. Samavat
Abstract
Due to the similarity in appearance and properties of some medicinal plants, it is necessary to identify them more precisely by various methods. Accordingly, in the present study, Satureja rechingeri Jamzad and S. khuzistanica Jamzad were investigated and compared based on morphological, phytochemical, ...
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Due to the similarity in appearance and properties of some medicinal plants, it is necessary to identify them more precisely by various methods. Accordingly, in the present study, Satureja rechingeri Jamzad and S. khuzistanica Jamzad were investigated and compared based on morphological, phytochemical, and molecular characteristics. S. rechingeri and S. khuzistanica seeds were collected from Ilam and Lorestan provinces, respectively, and after scientific identification, they were planted in the research greenhouse of the Research Institute of Forests and Rangelands. The seedlings were then transferred to the experimental farm of the mentioned institute based on a t-test (n=3). Several important morphological traits including inflorescence length, inflorescence stem internode length, length and calyx diameter, calyx three-large and two-short teeth length, length and corolla diameter, stamen length, stigma length, length and vegetative organ leaf width, length and leaflet width, stem diameter, plant height, number of main and sub-branches, largest and smallest canopy diameter, and length and reproductive organ leaf width were measured at full flowering stage in the third year of planting. The essential oils (EOs) were extracted from the plants floral branches in the third year of planting through water distillation. The EOs yield was calculated and their compounds were identified using ultra-fast gas chromatography (GC-FID). DNA barcoding and ITS marker were used for molecular studies on these two savory species. The results showed that these two species did not differ significantly (P<0.01) for all the morphological traits examined. 13 common compounds were identified in these two species EO. S. rechingeri and S. khuzistanica EOs contained 88.6% and 89.5% carvacrol, respectively. The EO yeild was obtained 3.3% for S. rechingeri and 3.04% for S. khuzistanica. These two species showed 100% nucleotide similarity with each other and were closely related to S. bachtiarica (98%). On this basis, it is probable that these two species are not only independent species, but can also be different accessions of the same species.
Agriculture and horticulture
A.A. Hatamnia
Abstract
Thymbra spicata L. (from Lamiaceae family) is distributed in large areas of Iran. In this study, the amount of total phenols and flavonoids, antioxidant activity, and percentage and essential oil composition in different ecotypes of this species from three different regions in Ilam province was evaluated. ...
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Thymbra spicata L. (from Lamiaceae family) is distributed in large areas of Iran. In this study, the amount of total phenols and flavonoids, antioxidant activity, and percentage and essential oil composition in different ecotypes of this species from three different regions in Ilam province was evaluated. The results showed that total phenols and flavonoids content was correlated with antioxidant activity positively and significantly, so that plant essential oil from Manesht Mountain with high phenols (8.6 mg gallic acid per g dry weight) and flavonoids (0.30 mg quercetin per g dry weight) content had the highest antioxidant activity, too. The main essential oil compound of the plant from regions with different ecological conditions was thymol (22.6-40.7%). Carvacrol, p-cymene, and γ-terpinene were the other main essential oil compounds of T. spicata from different regions. The results showed that the plant sample from Kaveran region and Manesht Mountain had the highest (2.4%) and lowest (1.4%) essential oil content, respectively. Overall, based on the results of the present study, it could be suggested that the essential oil percentage and composition of T. spicata depended not only to the genetic factors, but also to the ecological factors such as altitude, average annual temperature, and annual rainfall.
Agriculture and horticulture
M. Yadegari
Abstract
Due to the valuable role of thyme in the pharmaceutical and food industries of Iran, an experiment was conducted to study the effects of NPK complete fertilizers, butamisol, and humic acid on the morphophysiological characteristics and essential oil of three species of Thymus kotschyanus Boiss, T. vulgaris ...
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Due to the valuable role of thyme in the pharmaceutical and food industries of Iran, an experiment was conducted to study the effects of NPK complete fertilizers, butamisol, and humic acid on the morphophysiological characteristics and essential oil of three species of Thymus kotschyanus Boiss, T. vulgaris L., and T. daenensis Celark under drought stress conditions. This research was performed in three separate factorial experiments as a randomized complete block design with four replications in Islamic Azad University, Branch of Shahrekord in the 2021-2022 crop year. Foliar application was done twice with an interval of three weeks after the 10-leaf stage of the plant. Twenty-four experimental treatments included the non-use (A1) and use (A2) of NPK, non-use (B1) and use (B2) of humic acid, non-use (C1) and use (C2) of botamisol under drought stress conditions at three levels of field capacity (D1), 50% (D2), and 75% (D3) loss of gravitational water. The highest amounts of plant height (44.5±1.1 cm), number of main branches (26.4±1.5), plant dry weight (40.5±0.99 g.m-2), total chlorophyll (1.55±0.1 mg.g-1 fresh weight (FW)), and essential oil content (1.88±0.03%) were obtained in the A2B2C2D1 treatment in T. kotschyanus. The A2B2C2D3 treatment resulted in the highest proline content (13.9±0.2 µg.g-1 FW) and lowest essential oil content (0.79±0.1 %) in T. daenensis. Thymol and carvacrol in T. kotschyanus, thymol, ɣ-terpinene, p-cymene, and linalool in T. vulgaris, and thymol, carvacrol, p-cymene, β-caryophylene, α-pinene, and myrcene in T. daenensis were identified as the major essential oil compounds. More nutrient utilization and stress in the least possible along with the temperate climate of the study area led to the introduction of T. kotschyanus as an indicator species in this field.
Agriculture and horticulture
M. Yadegari
Abstract
Due to the valuable role of savory (Satureja spp.) in pharmaceutical and food industries, three separate factorial experiments were conducted in a completely randomized design with four replications in Islamic Azad University, Branch of Shahrekord in the 2021-2022 crop year to study the effects of NPK, ...
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Due to the valuable role of savory (Satureja spp.) in pharmaceutical and food industries, three separate factorial experiments were conducted in a completely randomized design with four replications in Islamic Azad University, Branch of Shahrekord in the 2021-2022 crop year to study the effects of NPK, butamisol, and humic acid fertilizers on the morphophysiological characteristics and essential oil of three savory species (S. bachtiarica Bunge, S. mutica Fisch. & C. A. Mey., and S. khuzistanica Jamzad) under drought stress conditions. Experimental treatments included non-use (A1) and use (A2) of NPK, non-use (B1) and use (B2) of humic acid, non-use (C1) and use (C2) of botamisol, and drought stress at three levels of field capacity (D1), and 50% (D2) and 75% (D3) moisture loss. The A2B2C2D1 treatment resulted in the highest plant height (57.5±1.1 cm), number of main branches (23.4±1.7), total chlorophyll (1.55±0.02 mg.g-1 FW), and essential oil content (1.94±0.03%) in S. bachtiarica. The highest proline (13.99±1.2 µg.g-1 FW) and lowest essential oil (0.79±0.02%) amounts was obtained in S. mutica in the A1B1C1D3 treatment. From the 11 essential oil compounds, monoterpenoids such as ρ-cymene and carvacrol in S. bachtiarica, carvacrol and ɣ-terpinene in S. khuzistanica, and ρ-cymene and thymol in S. mutica were identified as major essential oil constituents. Better nutrition and minimum stress with temperate climate of the region under study led to the introduction of S. bachtiarica as the best species.
Agriculture and horticulture
Z. Ebrahimi; M.R. Morshedloo; M.B. Hassanpour Aghdam
Abstract
To investigate the effects of different salinity levels on the essential oil content, and some morphological and physiological characteristics of two oregano subspecies (Origanum vulgare subsp. gracile (K.Koch) letsw. and O. vulgare subsp. hirtum (Link) letsw.), a factorial greenhouse experiment was ...
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To investigate the effects of different salinity levels on the essential oil content, and some morphological and physiological characteristics of two oregano subspecies (Origanum vulgare subsp. gracile (K.Koch) letsw. and O. vulgare subsp. hirtum (Link) letsw.), a factorial greenhouse experiment was conducted in a completely randomized design with four treatments and three replications. The salinity stress treatment was applied using sodium chloride in Hoagland nutrient solution at four levels of control (no stress), and mild (salinity 30 mM), moderate (salinity 60 mM), and severe (salinity 90 mM) stresses. According to the results, under salinity stress the growth indices, total carotenoids, and chlorophyll index decreased, but the activity of antioxidant enzymes and concentration of malondialdehyde and hydrogen peroxide increased in both subspecies. The wet and dry weight, carotenoids, hydrogen peroxide, and guaiacol peroxidase activity were obtained higher in subsp. hirtum than subsp. gracile. The percentage and essential oil composition were also affected by salinity. With increasing the salinity levels, the essential oil percentage was significantly increased compared to the control and its highest amount (0.8%) was observed under mild stress. Thirty five compounds were identified in both oregano subspecies essential oil. Carvacrol (30-72%: subsp. hirtum crarvacrol more than the other subsp.), γ-terpinene, carvacrol methyl ether, and p-cymene were the main components of both subspecies. The results of this study demonstrated the superiority of subsp. hirtum to the other oregano subspecies in terms of the tolerance to salinity stress and also quantitative and qualitative yields.
Agriculture and horticulture
H. Shabkhiz; A. Javanmard; A. Ostadi; M.R. Morshedloo
Abstract
To determine the effects of Myco-Root biofertilizer and different irrigation levels on the content and essential oil compounds of thyme (Thymus daenensis Celak.), a factorial experiment was conducted in a completely randomized design with eight treatments and three replications in the greenhouse of Plant ...
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To determine the effects of Myco-Root biofertilizer and different irrigation levels on the content and essential oil compounds of thyme (Thymus daenensis Celak.), a factorial experiment was conducted in a completely randomized design with eight treatments and three replications in the greenhouse of Plant Production and Genetics department, Faculty of Agriculture, University of Maragheh, Iran, in 2020. The factors included drought stress at four levels of without stress (irrigation at field capacity (FC) as a control), mild (irrigation at 75% FC), moderate (irrigation at 55% FC), and severe (irrigation at 35% FC) stresses and biofertilizer at two levels of inoculation and non-inoculation with Myco-Root. The results demonstrated that the fresh and dry weight of leaves, stem dry weight, total dry weight, leaf relative water content, chlorophylls a and b and total chlorophyll content, and root colonization percentage decreased significantly with increasing the water deficit level. However, the carotenoid content and essential oil percentage showed an increasing trend with increasing drought stress. The highest carotenoid content and essential oil percentage was obtained by using Myco-Root under moderate drought stress. Thymol, carvacrol, p < /em>-cymene, 1,8-cineole, (E)-caryophyllene, borneol, γ-terpinene, and α-pinene were identified as the major constituents of essential oil in the all treatments. The highest content of thymol was obtained under mild drought stress with the Myco-Root application. Furthermore, the highest content of carvacrol was recorded under mild, moderate, and severe drought stresses, respectively with the Myco-Root application. On average, the application of Myco-Root biofertilizer increased the content of thymol, carvacrol, 1,8-cineole, E-caryophyllene, borneol, and α-pinene by 4.19, 48.28, 15.93, 16.33, 14.15, and 3.78%, respectively compared to not using this biofertilizer. Overall, the results showed that some morphological and physiological traits of thyme decreased significantly with increasing the water deficit levels, but the application of Myco-Root biofertilizer could amend drought stress and improve the growth, percentage and essential oil quality of the plant. Thus, the use of Myco-Root biofertilizer instead of chemical fertilizers could be recommended to achieve the sustainable agriculture in the thyme cultivation.
Agriculture and horticulture
M. Nosrati Momvandi; E. Zeid Ali; M.J. Zarea; H. Mumivand; M. Kiani
Abstract
To investigate the effects of planting date (March 20 and April 21), full fertilizer (PLANTFEED) (zero and two per thousand), and harvest time (8 am, 12 noon, and 19 pm) on the quantitative and qualitative yield (leaves and stems dry weight, stems to leaves ratio, yield and percentage of essential oil, ...
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To investigate the effects of planting date (March 20 and April 21), full fertilizer (PLANTFEED) (zero and two per thousand), and harvest time (8 am, 12 noon, and 19 pm) on the quantitative and qualitative yield (leaves and stems dry weight, stems to leaves ratio, yield and percentage of essential oil, and carvacrol percentage) of Satureja rechingeri Jamzad, a factorial experiment was conducted in a randomized complete block design with three replications in 2017. Based on the results, all the mentioned traits except the stems to leaves ratio were affected by planting date and full fertilizer treatment. The planting date of April 21 and the application of full fertilizer increased all the studied traits except the carvacrol percentage and the stems to leaves ratio. The morning harvest time treatment increased the percentage and essential oil yield. The highest percentage of carvacrol (93.66%) was obtained in the triple interaction of planting date (March 20) × full fertilizer (two per thousand) × harvest time (evening) and the lowest one (82.77%) was obtained in the triple interaction of planting date (March 20) × no fertilizer application × harvest time (morning). The results showed that in the planting date of April 21 treatment, the application of full fertilizer caused better results in most of the mentioned traits. Also, the morning and evening harvest treatments caused the highest percentage and essential oil yield and carvacrol percentage. To investigate the effects of planting date (March 20 and April 21), full fertilizer (PLANTFEED) (zero and two per thousand), and harvest time (8 am, 12 noon, and 19 pm) on the quantitative and qualitative yield (leaves and stems dry weight, stems to leaves ratio, yield and percentage of essential oil, and carvacrol percentage) of Satureja rechingeri Jamzad, a factorial experiment was conducted in a randomized complete block design with three replications in 2017. Based on the results, all the mentioned traits except the stems to leaves ratio were affected by planting date and full fertilizer treatment. The planting date of April 21 and the application of full fertilizer increased all the studied traits except the carvacrol percentage and the stems to leaves ratio. The morning harvest time treatment increased the percentage and essential oil yield. The highest percentage of carvacrol (93.66%) was obtained in the triple interaction of planting date (March 20) × full fertilizer (two per thousand) × harvest time (evening) and the lowest one (82.77%) was obtained in the triple interaction of planting date (March 20) × no fertilizer application × harvest time (morning). The results showed that in the planting date of April 21 treatment, the application of full fertilizer caused better results in most of the mentioned traits. Also, the morning and evening harvest treatments caused the highest percentage and essential oil yield and carvacrol percentage.
F. Zare Mehrjerdi; S. Niknazar; A. Shahrokhi Racini
Abstract
Lead intoxication impairs oxidant/antioxidant balance that can be partially responsible for the toxic effects of lead in the various organs of body especially cardiovascular system. On the other hand, carvacrol, as major component of thyme plant, has a variety of properties including antioxidant ones. ...
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Lead intoxication impairs oxidant/antioxidant balance that can be partially responsible for the toxic effects of lead in the various organs of body especially cardiovascular system. On the other hand, carvacrol, as major component of thyme plant, has a variety of properties including antioxidant ones. The main objective of this study was to investigate the effects of carvacrol on blood pressure and some blood parameters in lead-poisoned rats. In this study, 40 male Wistar rats were randomly divided into five groups. The first group was control, the second group was poisoned with lead, and three lead poisoned groups receiving carvacrol at doses of 25, 50 and 100 mg/kg/daily. The blood pressure was monitored weekly by tail plethysmography coupled to a computer system. At the end of experiment, blood samples were obtained to assess some hematological parameters (RBC, Hb, Hct, and WBC). The mean blood pressure in lead exposed group was significantly higher than that of control group from the 21st day of the study. Carvacrol caused a decrease in hypertension in the lead poisoned rats. This deceasing was consistent throughout the experiment. Some blood parameters (RBC, Hb, Hct, and WBC) were decreased in lead exposed rats. These changes were prevented in the lead groups receiving carvacrol. According to the result of this study, it may be concluded that carvacrol could improve some lead induced changes in the cardiovascular system.
F. Sefidkon; Z. Jamzad; M.M. Barazandeh
Volume 20, Issue 4 , January 2018, , Pages 425-439
Abstract
The genus of Satureja with the persian name of “Marzeh” consists of 14 species in Iran, 9 of them are endemic. One of these endemic species is Satureja bachtiarica. In this research, the aerial parts of S. bachtiarica were collected from two different localities in Fars and Yazd provinces, ...
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The genus of Satureja with the persian name of “Marzeh” consists of 14 species in Iran, 9 of them are endemic. One of these endemic species is Satureja bachtiarica. In this research, the aerial parts of S. bachtiarica were collected from two different localities in Fars and Yazd provinces, at full flowering stage. The plant materials were hydro-distilled for obtaining their essential oils. The oils were analyzed by capillary GC and GC/MS. The oil yields were calculated equal to 2.15% for Yazd sample and 1.65% for Fars sample. Twenty compounds were identified in the oil of Fars sample with carvacrol (49.3%), p-cymene (12.7%) and trans-a-bergamotene (5.8%) as main components. Twenty compounds were characterized in the oil of Yazd sample with carvacrol (66.5%), p-cymene (15.2%) and linalool (4.6%) as main constituents. The relatively high amount of oil yield and high percentage of carvacrol in the oil of S. bachtiarica, showed that this species could have the medicinal and nutritional uses like S. hortensis and S. montana. These two Satureja species are not native of Iran and S. bachtiarica may be used instead of them.
A. Zarezadeh; F. Sefidkon; S.R. Tabaei Aghdaei; A. Mirhosseini; M.R. Arabzadeh; M.R. Mirjalili
Abstract
Essential oils of Satureja spp. contain valuable components, such as thymol and carvacrol. This research was carried due to evaluate essential oil quality and quantity of different accessions of Satureja species in cultivated condition., Seeds of 35 accessions of 10 Satureja species were collected from ...
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Essential oils of Satureja spp. contain valuable components, such as thymol and carvacrol. This research was carried due to evaluate essential oil quality and quantity of different accessions of Satureja species in cultivated condition., Seeds of 35 accessions of 10 Satureja species were collected from natural habitats and the seedlings were planted, using a randomized complete block design with for three replications at Medicinal Plants Research Station, Yazd, Iran. Aerial parts of plants were collected during four consecutive years, air dried in shadow and essential oils were extracted with hydrodistillation clevenger method. Essential oil compounds were identified by Gas chromatography (GC) and chromatography-mass spectroscopy (GC/MS). Analysis of variance showed significant differences at 1% between accessions for percentage of essential oil and essential oil yield per hectare. According to the results, accessions 15 (Satureja rechingeri) from Ilam province with 5.8% and 113.9 kg/ha essential oils, and 64% carvacrol and 12.2% thymol; accession 107 (S. spicigera) from Gillan province with 2.4% and 75.5 kg/ha essential oils, and 9.2% carvacrol and 43.4% thymol; accession 24 (S. rechingeri) from Ilam province with 4.2% and 63.8 kg/ha essential oils, and 75.4% carvacrol and 6.2% thymol; and SKM (S. bachtiarica ) from Yazd province with 2.6% and 51.5 kg/ha, essential oils, and 66% carvacrol and 0.5% thymol, respectively, showed the highest essential oil production in Yazd, Iran.
A. Zarezadeh; A. Mirhossaini; M. Mirza; Z. Jamzad; M. Arabzadeh
Abstract
Thymuskotschyanus Boiss. & Hohen. is a perennial medicinal shrub growing wild in some regions of Iran including Yazd province. This research was carried out in order to analyze oil quantity and quality of cultivated Thymuskotschyanus in the Medicinal Plants Farm, Yazd, Iran. The seeds of 19 accessions ...
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Thymuskotschyanus Boiss. & Hohen. is a perennial medicinal shrub growing wild in some regions of Iran including Yazd province. This research was carried out in order to analyze oil quantity and quality of cultivated Thymuskotschyanus in the Medicinal Plants Farm, Yazd, Iran. The seeds of 19 accessions collected from natural habitats were sown in a greenhouse and the seedlings were planted in the farm. Shoots were harvested at 50% flowering stage in the second year and dried under shadow. Essential oil was extracted with water distillation method and oil components were identified after determining oil content. The highest amounts of oil content respectively were related to accessions TK7 (3.42%), TK12 (3.22%), TK17 (2.66%) and the lowest amount was observed in accession TK6 (0.79%). The highest amounts of oil production respectively were recorded in accessions TK5 (38.3kg/ha), TK7 (36.4kg/ha) and TK12 (36.1 kg/ha) and the lowest amount was related to TK1 (1.3 kg/ha). Cluster analysis based on eleven oil components classified the accessions into three groups. The first group, (TK2, TK6, TK13, TK18, TK16, TK19), was rich for thymol-p-cymene, the scond group (TK1, TK3, TK4, TK5, TK7, TK11, TK14, TK15, TK17) for carvacrol-thymol and the 3rd group (TK8, TK9, TK10, TK12) was rich for Geraniol-linalool. In general, accessions TK7, with 3.42% oil content, 36.4 kg/ha oil production and 63.8% carvacrol; TK14, with 2.2% oil content 35.5 kg/ha oil production and 47.2% carvacrol and thymol, TK5 with 2.4% oil content, 38.3 kg/ha oil production and 57% thymol and carvacrol are proposed as superior accessions.
abbas zarezadeh; Fatemeh Sefidkon; S.R. Tabaei-Aghdaei; A. Mirhossaini; M.R. Arabzadeh; M.R. Mijalili
Abstract
Savory (Satureja) consists of 17 herbaceous annual and perennial species in Iran and Satureja rechingeri Jamzad is one of the endemic species in this country. This research was carried to study oil quantity and quality in S. rechingeri. Seeds of eight accessions, collected from natural habitats, were ...
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Savory (Satureja) consists of 17 herbaceous annual and perennial species in Iran and Satureja rechingeri Jamzad is one of the endemic species in this country. This research was carried to study oil quantity and quality in S. rechingeri. Seeds of eight accessions, collected from natural habitats, were sown in a greenhouse and the seedlings were planted in the Medicinal Plants Farm, Yazd, Iran. Essential oil was extracted from shadow dried flowering shoots with water distillation method and oil components were identified after determining oil content. Cluster analysis based on oil components classified the accessions into two groups, one of which was rich in carvacrol (SR1, SR2, SR3, SR5, SR7, SR8) and the other was rich in both carvacrol and thymol (SR6 and SR4). Based on the results of this study, SR4 with 5.85% essential oil, 113.9kg/ha oil yield, 64% carvacrol and 12.2% thymol , and SR6 with 4.22% essential oil, 63.8 8kg/ha oil yield, 75% carvacrol and 6.2% thymol are proposed as superior accessions.
A. Sepahvand; K. Khademi; H. Astereki; A. Mohammadian
Abstract
This research was aimed to study the effects of density on yield and yield components of thyme (Thymus lancifolius Celak.) under dry farming conditions of Lorestan province. The experiment was arranged in a randomized complete blocks design with three sowing density treatments (4, 6, and 8 plants per ...
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This research was aimed to study the effects of density on yield and yield components of thyme (Thymus lancifolius Celak.) under dry farming conditions of Lorestan province. The experiment was arranged in a randomized complete blocks design with three sowing density treatments (4, 6, and 8 plants per square meter) and three replications. The plant height, crown diameter, number of shoots, shoot dry yield, root length, and essential oil yield and content were measured. The results of combined analysis showed that the highest number of branches was obtained at a density of 8 plants per square meter as compared with 4 and 6 plants per square meter. According to the results, the dry matter yield and essential oil yield and content were calculated to be 3937.9 kg ha-1, 77.52 kg, and 1.973%, respectively. In addition, in the fourth year of study, the number of flowering branches, crown diameter and height were calculated to be 317.153, 42.87 cm and 15.3cm, respectively. The essential oil T. lancifolius contained more than 44% carvacrol and 31% thymol.
F. Sefidkon; R. Taebnia; M. Mirza
Abstract
Satureja species are aromatic plants, belonging to Lamiaceae family. The green parts and essential oils of Satureja species are used in medicinal and food industries. Satureja rechingeri Jamzad is an endemic species growing wild in Ilam province. In this research, the seeds of six populations were collected ...
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Satureja species are aromatic plants, belonging to Lamiaceae family. The green parts and essential oils of Satureja species are used in medicinal and food industries. Satureja rechingeri Jamzad is an endemic species growing wild in Ilam province. In this research, the seeds of six populations were collected from natural habitats for domestication of this species and study the essential oil content and composition in cultivated plants. The seeds were cultivated in the research farm of Research Institute of Forests and Rangelands in three replications in a complete randomized blocks design. For comparing the yields and constituents of essential oil, the aerial parts of each accession were collected in full flowering stage at three consecutive years. The plant materials were subjected to hydro-distillation after drying in room temperature. The oil yields were calculated and the oil compositions were identified by GC and GC/MS analysis and retention indices. Our results showed an increasing pattern for the oil yields of four populations during three years after cultivation and then it reached to 4.8%-6% in the third year. The major compound in all oils was carvacrol accounted for 80 to 88% in two- and three- year-old plants. According to the obtained results and improved yield of essential oil in cultivated samples compared to the wild ones as well as high levels of carvacrol, the cultivation of S. rechingeri in the same condition could be recommended.
F. Askari; E. Sharifi Ashorabadi; M. Mirza; M. Teimouri; E. Ehsani
Abstract
This research was aimed to investigate the effect of collection locality and essential oil concentration of Thymus pubescens Boiss. & Kotschy ex Celak on antimicrobial activity against some microorganisms. The statistical design used in this study was a factorial experiment in a completely randomized ...
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This research was aimed to investigate the effect of collection locality and essential oil concentration of Thymus pubescens Boiss. & Kotschy ex Celak on antimicrobial activity against some microorganisms. The statistical design used in this study was a factorial experiment in a completely randomized design with four replications. In this experiment, collection locality at six levels including the habitats of West Azarbaijan, Tehran, Zanjan, Qazvin, Kurdistan and Guilan, essential oil dilution at three levels including one fifth (1:5), one twenty-fifth (1:25), one fiftieth (1:50) and comparison with the antibiotics of ciprofloxacin and ceftizoxime, and studied microorganisms at five levels including Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeroginosa and Candida albicans were investigated. The seeds were collected from different habitats and cultivated in the field of Research Institute of Forests and Rangelands. The essential oil was isolated from shoots by hydro-distillation. Chemical compositions of the oils were analyzed by GC and GC/MS. The essential oil yield obtained from different localities varied between 0.39% to 0.83% (w/w). The major constituents of the essential oil obtained from the samples collected from West Azarbaijan, Tehran, Zanjan, Qazvin, Kurdistan and Guilan were as follows: West Azarbaijan: E-caryophyllene (26.0%) and camphor (24.2%); Tehran: geranial (30.9%) and geranyl acetate (23.9%); Zanjan: linalool (23.5%) and 1,8-cineol (22.2%); Qazvin: thymol (30.3%) and carvacrol (30.1%); Kurdistan: linalool (17.2%) and geranyl acetate (12.7%), and Guilan: α–terpineol (31.2%) and geraniol (11.2%). Analysis of variance of inhibition zone diameter of T. pubescence essential oil showed significant difference (p<0.05) among collection locality, microorganism type, essential oil dilution and their interaction. Mean comparisons of the collection locality, microorganism type and essential oil dilution showed that the highest inhibition zone diameter (35.5mm) was recorded for the essential oil obtained from West Azarbaijan, ciprofloxacin against Bacillus subtilis. The lowest inhibition zone diameter (8.0 mm) was observed in the essential oil obtained from West Azerbaijan with essential oil dilution of 1:50 against P. aeruginosa. According to the MIC and MBC results, the most and the least antimicrobial activity was recorded for the essential oils obtained from the seeds collected from Tehran and Kurdistan, respectively.
A. Zarezadeh; A. Mirhossaini; M. Mirza; M.R. Arabzadeh
Abstract
The essential oil of Thymus spp has valuable components such as thymol and carvacrol. The aerial parts of different thymus accessions, cultivated in Shahedied research station of medicinal plants, were collected at flowering stage and then dried in shadow and hydro-distilled for obtaining their essential ...
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The essential oil of Thymus spp has valuable components such as thymol and carvacrol. The aerial parts of different thymus accessions, cultivated in Shahedied research station of medicinal plants, were collected at flowering stage and then dried in shadow and hydro-distilled for obtaining their essential oils. Analysis and identification of chemical composition of the oil were performed by GC and GC/MS. Among 74 cultivated accessions in 2011, the highest amount of oil yield was related to the accessions CFTNJI (4.17%), ZELP (4%), Q (3.72%), NJICFT (3.71%), XDRMKO (3.54%), respectively and the lowest was related to BHUZSE (0.66%). The highest amount of oil production per hectare was related to the accesstions XDRVGY (87.5kg/ha), NJICFT(45.42kg/ha), ZSECFT(40.86kg/ha), BHUMKO (38.31kg/ha), respectively and the lowest was related to CFTMKO (0.9kg/ha). Para-cymene, 1,8-cineol, gamma terpinene, borneol, thymol, carvacrol, geraniol, limonene, acetate geranil, terpineol, linalool, caryophyllene, respectively with values of 28.41%, 31.66%, 20.44%, 29.35%, 71.1%, 81.45%, 76.99%, 43%, 28%, 38.6%, 55.56%, 81%, 20%, were related to the accessions VGYCFT, NJIBHU, MKOMKO, CFTCFT, ZSEQ, NJICFT, CFTQ, MKOQ, CFTZSE, NJIBHU, ZSEQ, NJICFT, CFTQ, MKOQ, CFTZSE, NJIBHU, ZSELP, VGYMKO. In (2012), the highest amount of oil yield was recorded for the accessions Q (4.07%), CFTVGY (3.7%), MKOVGY (3.63%), ZSEQ (3.45%), ZSELP (%3.43), respectively and the lowest was found in VGYMKO (62.0%). The highest amount of oil production per hectare was related to the accessions BHUVGY (74.9kg/ha), XDRVGY (60.9kg/ha), XDRLP (51.78kg/ha), CFT (49.21kg/ha), Q (46.11kg/ha), respectively and the lowest was found in BHUZSE(2.36kg/ha). Generally, in terms of oil yield , oil production and main composition of essential oil, three accessions including NJICFT (Thymus Transcaspicus) with 3.71% essential oil and 45.42kg per hectare oil production and 81.5 % carvacrol, ZSEQ (T. daenensis) with 3.45% essential oil and 37.15 kg/h oil production and 73% thymol, and XDRVGY (T. vulgaris) with 2.75% essential oil and 78.5kg/h oil production and 45.8% thymol were selected as superior accessions.
P. Haiati; V. Rowshan
Abstract
Satureja hortensis L. (Savory), belonging to the family Lamiaceae (formerly Labiatae) is an annual herb. In order to determine the effects of salicylic acid (SA) (Control, 200, 4oo and 600mgL-1) in early flowering stage on growth and components of essential oils of Satureja hortensis, a factorial ...
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Satureja hortensis L. (Savory), belonging to the family Lamiaceae (formerly Labiatae) is an annual herb. In order to determine the effects of salicylic acid (SA) (Control, 200, 4oo and 600mgL-1) in early flowering stage on growth and components of essential oils of Satureja hortensis, a factorial experiment based on randomized complete block design was carried out with three replications. The oil was analyzed by GC and GC/MS. The fresh weight was significantly increased in 200 mgL-1 SA, compared to the control. The yield of essential oil was 3.1% (Control) and 3.7% (200 mgL-1) with a significant difference. The main constituents of the essential oil were carvacrol, γ-terpinene, α-terpinene and p-cymene. SA application increased α-terpinene from 4.35% (Control) to 7.7% (200 mgL-1), β-myrcene from 2.5 (Control) to 4.3 (200 mgL-1), p-cymene from 1.9% (Control) to 2.5% (600 mgL-1) and decreased carvacrol from 44.8% (Control) to 38.5% (200 mgL-1). Results showed that SA application caused changes in the quantity and quality of essential oils.
L. Safaei; E Sharifi Ashoorabadi; H. Zeinali; D. Afiuni; M. Mirza
Abstract
In order to study the effects of harvesting stages on essential oil yield and composition of Thymus caramanicus Jalas, an experiment was conducted during 2007-2009 in Fozveh Research Station of Isfahan. collected seeds from natural habitat were cultivated in pots and then seedlings were transplanted ...
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In order to study the effects of harvesting stages on essential oil yield and composition of Thymus caramanicus Jalas, an experiment was conducted during 2007-2009 in Fozveh Research Station of Isfahan. collected seeds from natural habitat were cultivated in pots and then seedlings were transplanted at the main field in a randomized complete blocks design with 3 replications. Aerial parts of the species were harvested at 4 stages including: beginning of flowering, fifty percent flowering, full flowering and fruit set during two years. The plant materials were dried and their essential oils were obtained by water distillation. Capillary GC and GC/MS analysis were used to determine the volatile profile of essential oil samples. Results showed that harvesting stages had significant effects on thymol and carvacrol content and essential oil percentage. The highest amount of essential oil and thymol percentage were obtained at full flowering stage of the first year (1.56% and 3.59 kg/ha, respectively) and the maximum carvacrol percentage was obtained at fruit set stage of the first year (25.45 kg/ha). Six components consisted the highest amount of essential oil, among them carvacrol was dominant at all 4 harvesting stages. The highest amount of carvacrol was obtained at the beginning of flowering stage (88.45%) in the second year. Thymol was the second dominant component with a maximum percentage of 11.77%, obtained at full flowering stage. The highest content of ρ- cymene, 1,8-cineole and γ-terpinene were obtained at fruit set stage (2.35, 1.21 and 2.15%, respectively) and the highest content of borneol was recorded at full flowering stage (7.52%). Also, a positive correlation was observed between essential oil percentage and amounts of thymol and carvacrol. In conclusion, the best time for harvesting of T. caramanicus to achieve the maximum carvacrol percentage was fruit set stage, while full flowering stage was identified as the best time to achieve the maximum essential oil and thymol percentage.
Sh. Ahmadi; F. Sefidkon; P. Babakhanlo; F. Asgari; K. Khademi; N. Valizadeh; M.A. Karimifar
Abstract
The genus of Satureja consist of 30 species in world. Satureja bachtiarica Bunge is one of the endemic species of this genus in Iran. In order to identify the quality changes of essential oil of Satureja bachtiarica, we planted the specimen in 1382 in Khorram Abad on the 36 plot, using the split factorial ...
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The genus of Satureja consist of 30 species in world. Satureja bachtiarica Bunge is one of the endemic species of this genus in Iran. In order to identify the quality changes of essential oil of Satureja bachtiarica, we planted the specimen in 1382 in Khorram Abad on the 36 plot, using the split factorial design in randomized complete block with 3 replications. Aerial parts of plants were collected in two stages (before and full flowering) from field and provenance in the second year. The plant materials were dried and hydro-distilled in order to obtain their essential oils. The oils were analyzed by capillary GC and GC/MS. The main component of essential oil before flowering stage were carvacrol (20٪), P-cymene (36.5٪) and thymol (19.2.(٪ In the flowering stage 21 compounds were characterized in the oil with carvacrol (25.5٪), p-cymene (23.2٪), thymol (0.5(٪ and mentone (18.5 (٪as the main constituents. Fifteen compounds were characterized in the oil of field sample (Khorram Abad). The main constituents of the oil were carvacrol (48.6٪) and p-cymene (28.6٪) before flowering stage. In the flowering stage 13 compounds were characterized in the oil with carvacrol (62.3٪) and p-cymene (21.2٪) as the main constituents. The oil yields of cultivated and wild S. bachtiarica samples were 1.1, 2.08% and 1.8, 1.1% before and full flowering stages, respectively. The amount of carvacrol in Satureja bachtiarica oils were 48.6%, 62.3% and 20%, 25.8%before and full flowering stages, in field and provenance, respectively. These results showed that variation of ecological characters like altitude, temperature, humidity and climate had effect on percentage of carvacrol.
Kh. Abbasi; F. Sefidkon; Y. Yamini
Abstract
The genus Satureja from lamiaceae family consists of many species all over the world. In Iran, there are 15 species, 9 of them are endemic. The oil of different Satureja species are very important because of having carvacrol. In this study, the effect of two extraction methods (hydro-distillation and ...
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The genus Satureja from lamiaceae family consists of many species all over the world. In Iran, there are 15 species, 9 of them are endemic. The oil of different Satureja species are very important because of having carvacrol. In this study, the effect of two extraction methods (hydro-distillation and supercritical fluid extraction) on yield and composition of the essential oils Satureja hortensis and Satureja rechingeri wasinvestigated. The aerial parts of S. hortensis were collected from Research Station of Alborz (Karaj) at full flowering stage and those of S. rechingeri were collected from their natural habitats on Ilam province. After drying the plant material, the oil of both species were obtained by hydro-distillation and also by SFE (with and without modifier). The oil were analyzed by GC and GC/MS. 17 compounds were identified in the oil of S. hortensis by hydro-distillation, with carvacrol (48.1%) and g-Terpinene (38.4%) as the main components. In the oils of SFE, 9 compounds were characterized with carvacrol (57.1%-63.4%) and g-Terpinene (27.0%-32.8%) as the major components. Therefore, the percentage of carvacrol was increased by SFE and g-Terpinene was decreased. 20 components were identified in the oil of S. rechingeri by hydro-distillation, with carvacrol (86.6%) as main constituent. In the oils of SFE, 6 compounds were identified, that the percentage of carvacrol (95.0%-95.6%) was higher.
Sh. Mehrpur; F. Sefidkon; H. Mirzaie-Nodoushan; A. Majd
Volume 20, Issue 2 , July 2004, , Pages 159-169
Abstract
To investigate quality and quantity of essential oil of Thymus kotschyanus in greenhouse and field cultivation, seeds of four populations of this species from Azarbayejan, Sirachal, Mianeh and Tabriz were collected and cultivated in greenhouse and field condititions. Kelevenger method was ...
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To investigate quality and quantity of essential oil of Thymus kotschyanus in greenhouse and field cultivation, seeds of four populations of this species from Azarbayejan, Sirachal, Mianeh and Tabriz were collected and cultivated in greenhouse and field condititions. Kelevenger method was used to extract the essential oils from the plants foliage. Essential oil components were analysed and recognised by GC and GC/MS. The components included Thymol, Carvacrol and r-cymene.The highest main percentage of Thymol(65.94%), and r-cymene(20.40%), were found in Azarbayejan population and Carvacrol (53.14%), in Sirachal population. The oil yeilds of Thymus kotschyanus in field were higher than greenhouse conditions, but Thymol and Carvacrol were decreased and other monoterpenes were the major components of the essential oils in greenhouse conditions. In field conditions, the percentage of r-cymene was high, comparing to greenhouse condition, but g- terpinene was trace.This problem can be related to temprature and other conditions in field and greenhouse conditions. On the other hand, these differences between the oil composition of the greenhouse, and field grown populations, may reveal a common route of formation the components in the species.
F. Askari; F. Sefidkon; M. Mirza
Volume 19, Issue 2 , August 2003, , Pages 125-136
Abstract
One of the more distributed of The genus of Thymus is T. pubescens that is widespread in Tehran provinces up to 1800 m height and often widespread on flat and rocky surface. The aerial parts of Thymus pubescens were collected at four various localities in the Tehran province: Lar valley and Damavand ...
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One of the more distributed of The genus of Thymus is T. pubescens that is widespread in Tehran provinces up to 1800 m height and often widespread on flat and rocky surface. The aerial parts of Thymus pubescens were collected at four various localities in the Tehran province: Lar valley and Damavand (East of Tehran province), Sirachal and Fasham (Northwest of Tehran province). Essential oils were isolated by steam distillation from the plant material at two stages, before flowering (BF) and at full flowering (FF). The yields arranged between 0.34% and 0.86% at BF stage and between 0.40% and 2.03% at FF stage. In most sites of collection the oil content at BF stage was less than FF stage and the oil percentage of plant material from Lar valley and Damavand was more than of Sirachal and Fasham. At BF stage 25 compounds and at FF stage 26 compounds were characterized by means of GC and GC/MS. Major constituents were: carvacrol, thymol, g-terpinene, r-cymene, borneol, methy carvacrol, b-caryophyllene, 1,8-cineol + limonene and Geraniol. Twenty-three constituents were common at two stages. Geranyl acetate and thymyl acetate were found just at BF stage and geranial, nerol, and thymodihydroquinone were found just at FF stage.
M. Mazandarany; M.B. Rezaee
Volume 18, Issue 1 , February 2003, , Pages 111-122
Abstract
Fresh aerial parts of Thymus carmanicus JALAS scov. Nov are collected from charbagh montaines 2250 m from Gorgan state on mide of July 2001, essential oil extracted by steam distillation method. To produce oil on yields of 0.82% w/w. Essential oil were analyzed by GC and GC/MS. The major constituent ...
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Fresh aerial parts of Thymus carmanicus JALAS scov. Nov are collected from charbagh montaines 2250 m from Gorgan state on mide of July 2001, essential oil extracted by steam distillation method. To produce oil on yields of 0.82% w/w. Essential oil were analyzed by GC and GC/MS. The major constituent of the oil were, pulegone (25.37), 1.8- cineole (9.47), carvacrol (8.94), trans- p- menth- 2- en- l- oL (8.49), verbenone (8.04%), borneole (6.37%), p- cymene (6.13%), menthol (5.76%), piperitone (5.43%) and thymol (3%), being predominant.
F. Sefidkon; A. Rahimi-Bidgoly
Volume 15, Issue 1 , January 2003, , Pages 1-22
Abstract
The aerial parts of Thymus kotschyanus Boiss & Hohen were collected in three stages of plant growth (before, at the beginning and complete flowering) from research station of Sirachal. The essential oils of plant materials were isolated by steam-, hydro- and water-steam distillation from the aerial ...
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The aerial parts of Thymus kotschyanus Boiss & Hohen were collected in three stages of plant growth (before, at the beginning and complete flowering) from research station of Sirachal. The essential oils of plant materials were isolated by steam-, hydro- and water-steam distillation from the aerial parts. The oils were analyzed by capillary GC and GC-MS. So, the effect of distillation methods and stages of plant growth on oil content and composition of Thymus kotschyanus was investigated. The oil yield, related to distillation method and stage of plant growth, was 0.28-1.80% w/w (the highest for complete flowering stage by hydrodistillation method). The main constituents in all of the oils were carvacrol (46.74-61.23%), thymol (7.51- 26.92 %), g-terpinene, p-cymene and borneol.
M. Mirza; M. Najafpour navaei
Volume 15, Issue 1 , January 2003, , Pages 23-31
Abstract
The essential oil isolated by steam distillation from flowers of Oliveria decumbens Vent. and was analyzed By GC and GC/MS. Among the 14 Compounds identified, the major components were carvacrol (29.7%), Thymol (15.11%), γ-terpinene (20.46%) and P- cymene (15.40%).
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The essential oil isolated by steam distillation from flowers of Oliveria decumbens Vent. and was analyzed By GC and GC/MS. Among the 14 Compounds identified, the major components were carvacrol (29.7%), Thymol (15.11%), γ-terpinene (20.46%) and P- cymene (15.40%).
