Agriculture and horticulture
leili safaei; Fatemeh Sefidkon; saeed- davazdahemami-; Davood Aminazarm
Abstract
In order to study different methods of soil fertilization in Satureja .khuzistanica a research carried out in Fozveh station of Agricultural and Natural Resource Research Center of Isfahan, during 2016- 2020. The treatments were different levels of chemical fertilizers (N50P25K25), different levels of ...
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In order to study different methods of soil fertilization in Satureja .khuzistanica a research carried out in Fozveh station of Agricultural and Natural Resource Research Center of Isfahan, during 2016- 2020. The treatments were different levels of chemical fertilizers (N50P25K25), different levels of organic fertilizers (Cow Manure30, Cow Manure60 and 5 ton/ha Vermi-compost) and combination of chemical and organic fertilizers (N50P25K25+ Cow Manure30, N50P25K25+ Cow Manure60, N50P25K25+ 5 ton/ha Vermi-compost). These treatments were compared with the control. The project had 3 replications, according to a randomized complete block design. The percentage of essential oil was measured by water distillation (Clevenger) at 50% flowering stage. The amount of essential oil compounds was evaluated by gas chromatography (GC) and the qualification of essential oil was measured by Gas chromatography-mass spectrometry (GC-MS). According to the results, the maximum essential oil percentage and oil yeild in S. khuzistanica was 3.7% (in the third year) and 132.6 kg/ha (in the second year). Based on the results of the experimental years, the highest oil yield was observed in the combined treatment of 50 kg/ha nitrogen, 25 kg/ha of phosphorus and 25 kg/ha of potas plus 5 tons per hectare of vermicompost, in the third year. During the experimental years, 14 compounds were identified in S. khuzistanica essential oil that major compound was carvacrol (86.4-95.3%). According to the results, the qualitative yield of essential oil of this species was less affected by soil fertility treatments, but the quantitative yield of essential oil had a significant increase that indirectly increases the qualitative yield of essential oil. Finally, it was concluded that intermediate method was the superior treatment in order to improving quantitative yield and can lead to sustainable agriculture.
Agriculture and horticulture
A. Rafati; N. Valizadeh; F. Sefidkon; Y. Imani; F. Noormand Moaied
Abstract
Nepeta crassifolia Boiss. & Buhse, from Lamiaceae family, is an aromatic species with medicinal properties which is endemic to Iran. The seeds and aerial parts of this plant were collected from six different regions of East Azerbaijan province. After drying, the plant aerial parts essential oil was ...
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Nepeta crassifolia Boiss. & Buhse, from Lamiaceae family, is an aromatic species with medicinal properties which is endemic to Iran. The seeds and aerial parts of this plant were collected from six different regions of East Azerbaijan province. After drying, the plant aerial parts essential oil was obtained by hydrodistillation and their compounds were identified by GC and GC/MS. The collected seeds, after producing greenhouse seedlings, were planted in the field in a randomized complete block design with three replications. In the second year after planting, the flowering shoots were harvested, dried, and distilled. The results showed that there was significant differences among essential oil percentage of natural habitat samples which the highest amount belonged to "Marand" region, but the farm essential oil samples did not differ with each other significantly. The compounds nepetalactone I (4aα, 7α, 7aα) and II (4aα, 7α, 7aβ), globulol, germacrene B, caryophyllene oxide, and 1,8-cineole were identified as the main essential oil compounds of this species. The highest amount of nepetalactone I or II under the natural habitat (69% and 21.7%, respectively) and field (27.3% and 51.5%, respectively) conditions belonged to "Misho" region in "Marand". "Sarab" region also had the highest amount of nepetalactone II under the natural habitat and farm conditions compared to the other samples. The highest amount of 1,8-cineole (21.8%) in the oil was also obtained from the natural habitat of "Kharil" region.
Agriculture and horticulture
B. Yousefi; M.H. Lebaschy; F. Sefidkon; H. Safari
Abstract
To investigate the effects of planting density and organic fertilizers on the yield traits of Satureja spicigera (K.Koch) Boiss. under the Kermanshah rainfed conditions, an experiment was conducted as a split-plot based on complete randomized block design (CRBD) with three replications in Kermanshah ...
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To investigate the effects of planting density and organic fertilizers on the yield traits of Satureja spicigera (K.Koch) Boiss. under the Kermanshah rainfed conditions, an experiment was conducted as a split-plot based on complete randomized block design (CRBD) with three replications in Kermanshah Agricultural and Natural Resources Research Center in 2016-2020 crop years. The main factor and sub-factor were fertilizer (rotten cow manure, enriched straw, and field soil (control)) and plant density (2.66, 4, and 8 plants.m-2), respectively. To have the desired densities, plants were grown with a distance of 50 cm between the rows and 25, 50, and 75 cm on the rows. The results showed that the traits including diameter, height, canopy area, fresh and dry weight of plant, fresh and dry yield per hectare, and essential oil yield per hectare were significantly different between the years, plant densities, and fertilizer treatments. The highest fresh yield (3639.3 kg.ha-1) was obtained in the rotten cow manure+ high plant density treatment and the highest dry yield (2105 kg.ha-1) and essential oil yield (72.59 kg.ha-1) in the enriched straw+ high plant density one. The results showed that the best fertilizer and plant density treatment for S. spicigera was straw enriched with ammonium sulfate and 8 plants.m-2.
Agriculture and horticulture
B. Yoosefi; F. Sefidkon; M. Mirza; M.H. Lebaschy
Abstract
Satureja mutica Fisch. & C. A. Mey. is one of the native savory species of Iran that has received much attention due to its high essential oil content and phenolic compound of thymol. Determination the most suitable and best planting density as well as soil fertility is very important in increasing ...
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Satureja mutica Fisch. & C. A. Mey. is one of the native savory species of Iran that has received much attention due to its high essential oil content and phenolic compound of thymol. Determination the most suitable and best planting density as well as soil fertility is very important in increasing the quantitative and qualitative yield of medicinal plants. In this study, to investigate the effects of plant density and feeding with organic fertilizers on quantitative and qualitative yield of essential oils in Satureja mutica Fisch. & C. A. Mey. under rainfed conditions, an experiment was conducted as split plots in a randomized complete block design with 3 replications in the Research and Education Center of Agriculture and Natural Resources of Kermanshah province in 3 crop years (2018-2020). The main factor included 3 fertilizer treatments and the sub-factor was 3 densities (2.66, 4, and 8 plants per square meter) in rows with a distance of 50 cm and with 3 planting distances (25, 50, and 75 cm). Plant aerial parts were harvested at full flowering stage and their essential oil (EO) was extracted by hydrodistillation method. EO yield was calculated according to the plant dry weight and its chemical compounds were identified using GC and GC/MS. The results showed that percentage and EO chemical composition did not show any difference between the years. There was a significant difference between the fertilizer treatments for the percentage and EO yield and thymol, carvacrol, and other EO chemical compounds at the level of 1%. There was a significant difference between the planting densities only for EO yield. Thymol had a positive relationship with the treatments of enriched straw and then rotten cow manure and the highest amount (53.2%) was observed in the enriched straw treatment. Carvacrol with the highest amount of 9.2%, showed a positive relationship with the rotten cow manure treatment.
N. Saidi; H. Saderi; E. Taghian; F. Sefidkon; I. Rasooli; R. Mohammad Salehi; P. Owlia
Abstract
Pseudomonas aeruginosa is one of the most common opportunistic bacteria in nosocomial infections, which has a significant resistance to antimicrobials. Due to the restrictions in the use of antibiotics, the tendency to replace them with natural products has increased. In this study, the antimicrobial ...
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Pseudomonas aeruginosa is one of the most common opportunistic bacteria in nosocomial infections, which has a significant resistance to antimicrobials. Due to the restrictions in the use of antibiotics, the tendency to replace them with natural products has increased. In this study, the antimicrobial effect of four species of Satureja essential oils (S. mutica, S. bachtiarica, S. rechingeri and S. khuzestanica) on virulence factors of P. aeruginosa was evaluated. The minimum inhibitory concentration of Satureja essential oils was determined by microdilution broth method against standard strains of P. aeruginosa including PAO1 and 8821M. In the following, the effect of sub-minimum inhibitory concentrations (sub-MIC) of essential oils was investigated on virulence factors of this bacterium including motility, biofilm formation and alginate, elastase, and alkaline protease production of these two strains. All four Satureja essential oils had antimicrobial effects against the standard strains of P. aeruginosa, and also sub-MIC concentrations of the essential oils significantly reduced the virulence factors production of these strains. In this study, the suitable antagonistic effects of Satureja essential oils were observed against P. aeruginosa standard strains. By further study, these essential oils can be used as an antimicrobial compound against this bacterium.
F. Sefidkon; Z. Jamzad; M.M. Barazandeh
Volume 20, Issue 4 , January 2018, , Pages 425-439
Abstract
The genus of Satureja with the persian name of “Marzeh” consists of 14 species in Iran, 9 of them are endemic. One of these endemic species is Satureja bachtiarica. In this research, the aerial parts of S. bachtiarica were collected from two different localities in Fars and Yazd provinces, ...
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The genus of Satureja with the persian name of “Marzeh” consists of 14 species in Iran, 9 of them are endemic. One of these endemic species is Satureja bachtiarica. In this research, the aerial parts of S. bachtiarica were collected from two different localities in Fars and Yazd provinces, at full flowering stage. The plant materials were hydro-distilled for obtaining their essential oils. The oils were analyzed by capillary GC and GC/MS. The oil yields were calculated equal to 2.15% for Yazd sample and 1.65% for Fars sample. Twenty compounds were identified in the oil of Fars sample with carvacrol (49.3%), p-cymene (12.7%) and trans-a-bergamotene (5.8%) as main components. Twenty compounds were characterized in the oil of Yazd sample with carvacrol (66.5%), p-cymene (15.2%) and linalool (4.6%) as main constituents. The relatively high amount of oil yield and high percentage of carvacrol in the oil of S. bachtiarica, showed that this species could have the medicinal and nutritional uses like S. hortensis and S. montana. These two Satureja species are not native of Iran and S. bachtiarica may be used instead of them.
F. Nikkhah; F. Sefidkon; E. Sharifi Ashoorabadi
Abstract
The aerial parts of Thymus vulgaris were collected in three stages of plant growth (before flowering, at the beginning and full flowering stage) from Research Field of Shahid Beheshti University (Tehran) at spring. The plant materials were dried in shade and their essential oils were isolated by hydro-distillation ...
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The aerial parts of Thymus vulgaris were collected in three stages of plant growth (before flowering, at the beginning and full flowering stage) from Research Field of Shahid Beheshti University (Tehran) at spring. The plant materials were dried in shade and their essential oils were isolated by hydro-distillation in three replications. In addition, the essential oils of plant materials at full flowering stage were obtained by water-steam distillation and direct steam distillation. The oil yields were calculated based on dried weigh and compared by statistic analysis. The oils were analyzed by capillary GC and GC/MS. Statistic analysis showed different stages of plant growth and different distillation methods had significant effect on oil yield. The highest oil yield was obtained at the beginning of flowering (1.18% w/w). Among distillation methods, the highest oil yield was obtained by direct steam distillation (1.20%). Analysis and identification of components showed thymol, P-cymene and g-terpinene as main compounds in all samples. The percentage of thymol at the beginning and full flowering stage was the same, but at the beginning of flowering was lower. There was no remarkable difference between different distillation methods for producing thymol, but by hydro-distillation the thymol amount was little higher. Based on the results of this investigation it can be concluded that the beginning of flowering and hydro-distillation are the best for obtaining the higher oil content and thymol percentage.
E. Fathi; F. Sefidkon; Gh. Bakhshi Khaniki; Z. Abravesh; M.H. Assareh
Volume 25, Issue 1 , May 2009, , Pages 64-74
Abstract
In this research, the fresh leaves of Eucalyptus largiflorens were collected in the middle of spring from Kashan in Isfahan province. After drying the plant materials in oven 30°C, 40°C, 50°C, sun and shade, their essential oils were obtained by hydro-distillation. In addition, the essential ...
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In this research, the fresh leaves of Eucalyptus largiflorens were collected in the middle of spring from Kashan in Isfahan province. After drying the plant materials in oven 30°C, 40°C, 50°C, sun and shade, their essential oils were obtained by hydro-distillation. In addition, the essential oil of shade-dried sample was obtained by two other distillation methods (water and steam distillation and direct steam distillation). The oils were analyzed by GC and GC/MS. Oil yields (w/w) of the oven-dried 30°C, 40°C, 50°C, sun-dried and shade-dried sample were 1.37%, 1.58%, 1.59%, 1.31% and 1.32% respectively. 1,8-cineole (25.0%, 26.2%, 40.6%, 29.7% and 24.6%), P-cymene (17.2%, 17.4%, 20.3%, 20.5% and 17.1%) and α-pinene (16.2%, 12.6%, 14.4%, 7.2% and 9.9%) were identified as the main components in oven-dried 30°C, 40°C, 50°C, sun-dried and shade-dried sample, respectively. On the other hand, the oil yields were 0.92% and 0.77% in water and steam distillation and direct steam distillation. In different drying methods the result showed the oil yield and 1,8-cineole percentage were higher in oven-dried 50°C sample and in different distillation the oil yield in hydro-distillation was higher and the highest percentage of 1,8-cineole was obtained by water and steam distillation.
Z. Jalali; F. Sefidkon; M.H. Assareh; F. Attar
Volume 24, Issue 1 , May 2008, , Pages 31-37
Abstract
Chamazolene, a-bisabolene oxide A and a-bisabolol oxide A and B are important and valuable sesquiterpens in the flowers of Anthemis and Matricaria genus that are noticed because of their different usage in cosmetic and hygiene materials. In this research, the flowers of 3 specimens of Matricaria recutita ...
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Chamazolene, a-bisabolene oxide A and a-bisabolol oxide A and B are important and valuable sesquiterpens in the flowers of Anthemis and Matricaria genus that are noticed because of their different usage in cosmetic and hygiene materials. In this research, the flowers of 3 specimens of Matricaria recutita L. (from Khuzestan, Booshehr and Fars provinces), M. aurea (Loefl.) Schultz-Bip.(fromTange eram) and Anthemis hyalina DC.(from Dashtestan) were collected. After drying the flowers in room temperature, the essential oils were obtained by hydro-distillation. Chemical composition of the oils were investigated by analytical GC, GC/MS and computing retention indices. The oil yield of M. recutita (Booshehr) was 2.85%, in specimen of Khuzestan 0.68% and in specimen ofFarswas 2.35%, in M. aurea 0.63% and in A. hyalina was 2.54%. Nine compounds were recognized in different specimens of M. recutita that their main components were a-bisabolene oxide A (63.4%-92.4%), a-bisabolol oxide A (3.3%-15.4%) and chamazolene (2.6%-10.6%). Seven compounds were identified in M. aurea that a-bisabolene oxide A (59.0%), a-bisabolol oxide A (22.3%) and chamazolene (8.8%) were main components. Six compounds were recognized in essential oil of A. hyalina that major compounds were a-bisabolene oxide A (66.1%), a-bisabolol oxide A (15.5%) and chamazolene (6.9%).
F. Sefidkon; L. Sadeghzadeh; M. Teimouri; F. Asgari; Sh. Ahmadi
Volume 23, Issue 2 , August 2007, , Pages 174-182
Abstract
The genus Satureja represents 15 species in Iran, 9 of them are endemic. In this study, the aerial parts of Satureja khuzistanica Jamzad and Satureja bachtiarica Bunge were collected at two stage of plant growth (before flowering and full flowering) from their natural habitats. After drying the plant ...
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The genus Satureja represents 15 species in Iran, 9 of them are endemic. In this study, the aerial parts of Satureja khuzistanica Jamzad and Satureja bachtiarica Bunge were collected at two stage of plant growth (before flowering and full flowering) from their natural habitats. After drying the plant materials in shade, essential oils were obtained by hydro-distillation and analyzed by capillary gas chromatography, using flame ionization and mass spectrometric detection. The results showed the oil of S. bachtiarica contained 20% carvacrol and 19% thymol before flowering and 26% carvacrol and 5% thymol at full flowering stage, as main components. The oil of S. khuzistanica, in both harvesting time contained about 90% carvacrol. Due to the antimicrobial effect of phenolic compounds, thymol and carvacrol, the antimicrobial effects of these oils were determined against five gram positive bacteria (Bacillus subtilis, Bacillus cereus, Micrococcus loteus, Staphylococcussp. and Staphylococcus areous) and three gram negative bacteria (Kellebsiella pneumonia, Kellebsiella oxytoca and Pseudomonas aeroginosa). The result showed the oil of S. khuzistanica had strong anti-bacterial effect in both harvesting stage. The anti-bacterial effect of S. bachtiarica oil was stronger before flowering stage, because of more percentage of phenolic compounds. So these oils can be used instead of synthetic antibiotics that their resistance against bacteria increased daily.
F. Sefidkon; M.H. Assareh; M. Mirza; Z. Abravesh; M.H. Salehe Shushtari
Volume 23, Issue 1 , May 2007, , Pages 39-50
Abstract
In this investigation, the leaves of five Eucalyptus species as Eucalyptus microtheca F. Muell, E. gongylocarpa Blakely, E. gillii Maiden, E. woodwardi Maiden and E. salubris F. Muell were collected from two regions of Khuzistan province (Shushtar and Dezful, South Iran) in spring. After drying the plant ...
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In this investigation, the leaves of five Eucalyptus species as Eucalyptus microtheca F. Muell, E. gongylocarpa Blakely, E. gillii Maiden, E. woodwardi Maiden and E. salubris F. Muell were collected from two regions of Khuzistan province (Shushtar and Dezful, South Iran) in spring. After drying the plant materials in shade, their essential oils were obtained by hydro-distillation. The oils were analyzed by capillary gas chromatography, using flame ionization and mass spectrometric detection. The oils of E. microtheca were obtained at yields of 0.43% and 0.37% (w/w) from Shushtar and dezful, respectively. These yields were 2.37% and 1.78% for E. gongylocarpa, 3.5% and 3.9% for E. gillii, 1.18% and 1.13% for E. woodwardi, 2.05% and 1.02% for E. salubris. The main compounds of E. microtheca oils were 1,8-cineole (26.7% and 16.9%), P-cymene (14.8% and 20.4%) and a-pinene (12.5% and 12.0%) in the Shushtar and Dezful samples, respectively. The major constituents of E. gongylocarpa oils were 1,8-cineole (66.8% and 78.6%) and a-pinene (5.2% and 7.2%). The main components of E. gillii oils were 1,8-cineole (58.5% and 81.3%) and a-pinene (13.9% and 11.0%). The main compounds of E. woodwardi oils were 1,8-cineole (58.7% and 45.1%) and a-pinene (23.1% and 21.4%). The major constituents of E. salubrisoils were 1,8-cineole (62.0% and 71.0%) and a-pinene (8.0% and 10.0%). Differences in the percentage of the main components of these oils in two regions could be due to some differences in habitats like soil structure or humidity.
F. Sefidkon; Z. Akbari; M.H. Assareh; Gh. Bakhshi Khaniki
Volume 22, Issue 4 , February 2007, , Pages 351-365
Abstract
Rosa damascena Mill is one of the important Rose species for production of aromatic compounds. The oil and distilled water of Rose are used vastly in medicinal, hygienic-cosmetic and food industries. In Iran, there are vast gardens of Rosa damascena in Kashan, Kerman, Tabriz, Sahand and Fars province. ...
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Rosa damascena Mill is one of the important Rose species for production of aromatic compounds. The oil and distilled water of Rose are used vastly in medicinal, hygienic-cosmetic and food industries. In Iran, there are vast gardens of Rosa damascena in Kashan, Kerman, Tabriz, Sahand and Fars province. In this research, the effect of different extraction methods on yield and chemical composition of four Rosa damascena samples (two samples from national botanical garden of Iran with source of Kashan and Oskou, one sample from Kashan and one sample from Chaloos road) were examined. The aromatic compounds were obtained by four extraction methods consisted of two distillation methods (hydro-distillation and water & steam distillation), extraction with organic solvents (by use of hexane and petroleum ether, individually). The yields of essential oils (from distillation methods) and concrete and absolute (from solvent extraction) were calculated. The oils and absolutes were analyzed by GC and GC/MS. The result showed (except Oskou sample) there is no significant difference between oil and absolute yield, but for all samples, the yield of concrete was higher than the yield of oil and absolute, significantly. Distillation methods produced higher percentage of citronellol and sometimes geraniol, but the valuable compound, phenyl ethyl alcohol, was not found in the oils or exist in very little amount. In solvent extraction methods, the percentage of phenyl ethyl alcohol was considerably high and citronellol and also geraniol were found in the absolutes, of course in lower amounts. There were also some differences between the minor components.
F. Askari; F. Sefidkon; Sh. Ahmadi
Volume 22, Issue 2 , September 2006, , Pages 98-104
Abstract
trans-a-Bergamotene is an aromatic compound that is used for synthesize of the other aromatic compounds. Pimpinella affinis Ledeb. grows wild in different regions of Iran, north, north-west, center, eastern and north-east. In This research, the aerial parts and seeds of P. affinis werecollected ...
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trans-a-Bergamotene is an aromatic compound that is used for synthesize of the other aromatic compounds. Pimpinella affinis Ledeb. grows wild in different regions of Iran, north, north-west, center, eastern and north-east. In This research, the aerial parts and seeds of P. affinis werecollected from Noshahr in 2003 and 2004. Essential oils were isolated by hydro-distillation. The yields of stem plus the leaf, inflorescence and seed oils for the first year were 0.26%, 1/1% and 4.1% w/w and for the second year were 0.26%, 0.86% and 2.45%, respectively. Four constituents in stem plus the leaf oil, five constituents in inflorescence oil and six constituents in seed oil were identified in samples of 2003. Six constituents in stem plus the leaf oil, fifteen constituents in inflorescence oil and six constituents in seed oil were identified in samples of 2004. Major constituent of the stem plus the leaf, inflorescence, and seed oils was trans-a-Bergamotene (91.1%, 96.2% and 90.2% in 2003 and 94.3% 84.9% and 95.5% in 2004, respectively).
E. Alishahi-Noorani; F. Sefidkon; M. Yoosefzadi; S. Neamati; M. Khajeh-piri
Abstract
The Genus Salvia from Labiatae family consists of 700 species in the world, 58 species in Iran. The plant materials of S. chloroleuca were collected from 18 km on Chalus. The Genus Nepeta from Labiatae family has vast distribution in all over of Iran. The aerial parts of N. fissa were collected from ...
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The Genus Salvia from Labiatae family consists of 700 species in the world, 58 species in Iran. The plant materials of S. chloroleuca were collected from 18 km on Chalus. The Genus Nepeta from Labiatae family has vast distribution in all over of Iran. The aerial parts of N. fissa were collected from 4km on Ab-Ali. In this research, at first the essential oils were extracted by hydro-distillation from the aerial parts of S. chloroleuca and N. fissa. The oils were analyzed by capillary GC and GC/MS. Twelve compounds were identified in the oil of S. chloroleuca with Germacrene D (37.4%) β-cayophyllene (37%) and bicyclogermacrene (7.1%) as Main constituent. Forty-two components were characterized in the oil of N. fissa with Caryophyllene oxide (12.3%) Valencene (6.6%) and β- pinene(6%) as main compound. Then the antimicrobial activities of the essential oils were evaluated by disk diffusion method using: Bacillus subtilis (G+), Staphylococus aureus (G+) ATCC 25923, Pseudomonas aeruginosa (G-) ATCC 85327, Echerchia coli (G-) ATCC 25992. The results indicated that the oil of S. chloroleuca had the greatest antibacterial effect against mentioned bacteria, on E. coli and S. aureus were mid- resistant.
F. Sefidkon
Volume 20, Issue 2 , July 2004, , Pages 149-158
Abstract
The genius of Echinophora presents 4 aromatic species in Iran, two of them are endemic. In this research, the aerial parts of E. sibthorpiana were collected in full flowering stage, from Tehran Provinc (north of Tehran). The plant materials were dried in room temprature and the essential oil isolated ...
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The genius of Echinophora presents 4 aromatic species in Iran, two of them are endemic. In this research, the aerial parts of E. sibthorpiana were collected in full flowering stage, from Tehran Provinc (north of Tehran). The plant materials were dried in room temprature and the essential oil isolated by steam distillation. The oil was analyzed by capillary GC and GC/MS. Among 17 compounds identified, the major components were delta-3-carene (31.8%), alpha-phellandrene (31.0%), methyl eugenol (16.9%), beta-phellandrene (5.3%) and p-cymene (4.7%). This oil could be used in cosmetic-hygenic industries because of the high amounts of delta-3-carene and alpha-phellandrene. These compounds have very good smells and used in perfumes.
Sh. Mehrpur; F. Sefidkon; H. Mirzaie-Nodoushan; A. Majd
Volume 20, Issue 2 , July 2004, , Pages 159-169
Abstract
To investigate quality and quantity of essential oil of Thymus kotschyanus in greenhouse and field cultivation, seeds of four populations of this species from Azarbayejan, Sirachal, Mianeh and Tabriz were collected and cultivated in greenhouse and field condititions. Kelevenger method was ...
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To investigate quality and quantity of essential oil of Thymus kotschyanus in greenhouse and field cultivation, seeds of four populations of this species from Azarbayejan, Sirachal, Mianeh and Tabriz were collected and cultivated in greenhouse and field condititions. Kelevenger method was used to extract the essential oils from the plants foliage. Essential oil components were analysed and recognised by GC and GC/MS. The components included Thymol, Carvacrol and r-cymene.The highest main percentage of Thymol(65.94%), and r-cymene(20.40%), were found in Azarbayejan population and Carvacrol (53.14%), in Sirachal population. The oil yeilds of Thymus kotschyanus in field were higher than greenhouse conditions, but Thymol and Carvacrol were decreased and other monoterpenes were the major components of the essential oils in greenhouse conditions. In field conditions, the percentage of r-cymene was high, comparing to greenhouse condition, but g- terpinene was trace.This problem can be related to temprature and other conditions in field and greenhouse conditions. On the other hand, these differences between the oil composition of the greenhouse, and field grown populations, may reveal a common route of formation the components in the species.
F. Askari; F. Sefidkon
Volume 20, Issue 2 , July 2004, , Pages 229-237
Abstract
Melissa officinalis L. is a very easily grown plant that habitats in south and Eastern of Europe, Spain and Asia. The plant of Melissa collected from three fields in Fars, Tehran and Semnan provinces. Essential oils were isolated by hydro-distillation. The yields of Essential oils of Fars sample (Shiraz), ...
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Melissa officinalis L. is a very easily grown plant that habitats in south and Eastern of Europe, Spain and Asia. The plant of Melissa collected from three fields in Fars, Tehran and Semnan provinces. Essential oils were isolated by hydro-distillation. The yields of Essential oils of Fars sample (Shiraz), Tehran sample (Karaj) and Semnan sample were 0.14%, 0.25% and 0.26% (w/w) respectively. The oils were analyzed by GC (9A-Shimadzu) and GC/MS (Varian 3400). Seventeen constituents at Fars sample oil, seventeen constituents at Tehran sample oil and fourteen constituents at Semnan sample oil were identified respectively. Major constituents of Fars sample were citronellal (25.4%), b-caryophyllene (11.3%), thymol (10.5%), globolol (6.1%), caryophyllene oxide (6.5%) and geraniol (5.7%). Major constituents of Karaj sample oil were citronellal (9.3%), carvacrol (31.8 %), Humulene epoxide (25.4 %) and a-humulene (10.2%). Major constituents of Semnan samples oil were citronellal (43.8%), b-caryophyllene (13.5%), thymol (7.9%), globolol (6.8%), neral (5.3%) and geranial (5.0%). Fourteen constituents were common at all sample oil. Major constituent at all samples oil was citronellal. Four major constituents were common at Fars and Semnan samples oil.
F. Sefidkon; A. Agha-Vali Jamaat; M. Alinia Rodsari; K. Jimand
Volume 20, Issue 1 , April 2004, , Pages 19-38
Abstract
Robinia pseudoacacia (Black locust ) is an ornamental tree with beautiful flowers. It,s main distribution is North America which spreads to Europe , Asia and Iran. Today, it is naturalized in Iran and grows in every where. Previously, we reported ...
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Robinia pseudoacacia (Black locust ) is an ornamental tree with beautiful flowers. It,s main distribution is North America which spreads to Europe , Asia and Iran. Today, it is naturalized in Iran and grows in every where. Previously, we reported chemical composition of the essential oil and absolute of hexan extract of R. pseudoacacia. In this research, the flavonoids from flowers and leaves of Robinia pseudoacacia were investigated. The extracts of flowers and leaves of R. pseudoacacia were obtained by Sochselet apparatus (by petroleum ether, benzen, chlorform, acetone and methanol) and also by macceration in methanol-water (9:1) and then methanol-water (1:1) and extraction with chlorform and hexan. After purification and separation of the extracts by P.C, HPLC, column and flash chromatography, two flavonoids, Quercetine and Robinine were obtained as pure compounds. Structure of the compounds were charachterized by HPLC (using standards), U.V. and 1HNMR .
F. Askari; F. Sefidkon; M. Mirza; S. Meshkizadeh
Volume 19, Issue 3 , October 2003, , Pages 239-254
Abstract
The genus of Pimpinella presents about 20 species, which are found wild in different regions of Iran. Most of them are annual. Two of the more distributed and prenial of these species is P. eurea and P. tragium. Essential oils were isolated by hydro-distillation from the seeds, flowers, and stems of ...
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The genus of Pimpinella presents about 20 species, which are found wild in different regions of Iran. Most of them are annual. Two of the more distributed and prenial of these species is P. eurea and P. tragium. Essential oils were isolated by hydro-distillation from the seeds, flowers, and stems of Pimpinella aurea DC.Individually, that were collected from Fasham (Northwest of Tehran province) and Tochal (North of Tehran province). The yields of seeds, flowers, and stems oils of Fasham sample were 1.97%, 1.54% and 0.44% w/w, and the yields of seeds, flowers, and stems oils of Tochal samples were 1.2%, 0.47% and 0.48% w/w, respectively. The oils were analyzed by GC (9A-Shimadzu) and GC/MS (Varian 3400). Eight and four constituents at seed oil, thirty-two and thirty-four constituents at flower oil and eighteen and twenty constituents at stem oil of Fasham and Tochal samples were identified respectively. In the Fasham samples: Major constituents of the stem plus the leaf oil were: 1,8-Cineol and Limonene (21.4%), Viridiflorol (12.8%), a-Pinene (11.5%) and Kessane (10.5%). Major constituents of the flower oil were: Viridiflorol (32.5%) and b-Bisabolene (29.5%). Major constituents of the seed oil were: b-Bisabolene (50.8%) and Viridiflorol (37.0%). In the Tochal samples: Major constituents of the stem plus the leaf oil were: b-Bisabolene (18.3%), Geranyl acetate (14.7%), Geranyl2-methyl butyrate (9.0%) and Limonene (7.3%). Major constituents of the flower oil were: b-Bisabolene (55.2%) and a-zingiberene (8.7%). Major constituents of the seed oil were: b-Bisabolene (76.5%) and Caryophyllene oxide (21.4%).
F. Sefidkon; R. Kalvandi; M. Mirza
Volume 19, Issue 3 , October 2003, , Pages 255-267
Abstract
The genus of Nepeta presents 67 species in Iran, most of them are endemic. One of theses endemic species is N. heliotropifolia. In this research the essential oil of N. heliotropifolia was investigated in three different stage of plant growth. The aerial parts of N. heliotropifolia at before flowering, ...
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The genus of Nepeta presents 67 species in Iran, most of them are endemic. One of theses endemic species is N. heliotropifolia. In this research the essential oil of N. heliotropifolia was investigated in three different stage of plant growth. The aerial parts of N. heliotropifolia at before flowering, beginning of flowering and complete flowering were collected from Hamedan province. Essential oils isolated by hydro-distillation from the aerial parts of three samples, were analyzed by a combination of capillary GC and GC/MS. The yields of essential oils were obtained 0.75%, 0.63% and 0.4% (w/w) respectively. So the oil yield of N. heliotropifolia was decreased from before flowering stage to complete flowering stage. Thirty-six components were identified in the oils. The main constituent of the essential oil at before flowering stage was limonene (40.1%), that decreased by plant growth. The second main component of the oil in this stage was b-pinene (11.1%), that increased to 23.3% in the beginning of flowering, then decreased in fool flowering. The other main compounds of the oils were 1,8-cineole, b-caryophyllene, (Z)- b-farnesene, g-mmurolene abd bicyclogermacrene.
F. Askari; F. Sefidkon; M. Mirza
Volume 19, Issue 2 , August 2003, , Pages 125-136
Abstract
One of the more distributed of The genus of Thymus is T. pubescens that is widespread in Tehran provinces up to 1800 m height and often widespread on flat and rocky surface. The aerial parts of Thymus pubescens were collected at four various localities in the Tehran province: Lar valley and Damavand ...
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One of the more distributed of The genus of Thymus is T. pubescens that is widespread in Tehran provinces up to 1800 m height and often widespread on flat and rocky surface. The aerial parts of Thymus pubescens were collected at four various localities in the Tehran province: Lar valley and Damavand (East of Tehran province), Sirachal and Fasham (Northwest of Tehran province). Essential oils were isolated by steam distillation from the plant material at two stages, before flowering (BF) and at full flowering (FF). The yields arranged between 0.34% and 0.86% at BF stage and between 0.40% and 2.03% at FF stage. In most sites of collection the oil content at BF stage was less than FF stage and the oil percentage of plant material from Lar valley and Damavand was more than of Sirachal and Fasham. At BF stage 25 compounds and at FF stage 26 compounds were characterized by means of GC and GC/MS. Major constituents were: carvacrol, thymol, g-terpinene, r-cymene, borneol, methy carvacrol, b-caryophyllene, 1,8-cineol + limonene and Geraniol. Twenty-three constituents were common at two stages. Geranyl acetate and thymyl acetate were found just at BF stage and geranial, nerol, and thymodihydroquinone were found just at FF stage.
F. Sefidkon; S. Meshkizadeh; S. Shahrzad
Volume 18, Issue 1 , February 2003, , Pages 23-42
Abstract
Sequoia is a very big and ever green tree with 45-90 meter height. It is native to the side of Atlantic Ocean, Which is also cultivated in Iran. The plant materials were collected from one of the Sequoia trees from Rezvanshahr Garden in August. The height to this tree was 40 meter and it was 30 years ...
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Sequoia is a very big and ever green tree with 45-90 meter height. It is native to the side of Atlantic Ocean, Which is also cultivated in Iran. The plant materials were collected from one of the Sequoia trees from Rezvanshahr Garden in August. The height to this tree was 40 meter and it was 30 years old. For preparation of tissue culture samples, the end part of shoots in 1-2 centimeter length have been cultivated. The leaves from main tree and also tissue culture sample were hydro- distilled for their essential oils. The oils were analyzed by combination of capillary GC and GC/MS. 72 Compounds were identified in the leaf oil of main tree and 37 compounds were characterized in the leaf oil of tissue culture sample. The main components of the oil from main tree were β- phellandrene and limonene (13.30%), α- pinene (6.83%), terpinene –4-ol (6.47%), g-teroubebe *5.44%) and germacrene B (4.17%), while the major components of the oil from tissue culture sample were α- pinene (26.30%), α- terpinenyl acetate (14.40%), sabinene (13.60%), g- terpinene (7.10%), germacrene D (6.90%), b-phellandrene and limonene (6.60 %) and p-cymene (5.00%). Althoug these components were found in both oils, but the higher percentage of some useful compounds in the oil of tissue culture sample, gave it more effective character.
A. Akbarinia; A. Ghalavand; Z. Tahmasebnbi; F. Sefidcon; E. Sharifi; M.B. Rezaii
Volume 18, Issue 1 , February 2003, , Pages 89-109
Abstract
This experiment was conducted on 2001 and 2002 in reseach farm of Qazvin Agriculture Research Station, in order to study the effect of different rates of nitrogen, Phosphorus (Conventional system), manure (Organic system) and mixture of them (Integrated system) on seed yield and essential oil content ...
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This experiment was conducted on 2001 and 2002 in reseach farm of Qazvin Agriculture Research Station, in order to study the effect of different rates of nitrogen, Phosphorus (Conventional system), manure (Organic system) and mixture of them (Integrated system) on seed yield and essential oil content of Ajowan (Carum copticum). The experimental design was used randomized complete blocks with three replication. Treatments included: different rates of N and P (as mixture) with 4 levels, different rates of manure with 3 lebvels, mixture of N, P and distillation method. The results showed that increase in N and P fertilizer up to 90 and 60 kg/ha respectively, was followed by seed yield increase but fertilizers had no effect on the essential oil content. Also, seed yield essential oil content increased as a result of increase in manure application. In general integrated treatments in respect of seed yeild and essential oil yield were higher than other treatments.
F. Sefidkon
Volume 11, Issue 1 , February 2002, , Pages 73-85
Abstract
The essential oil isolateed by water and steam distillation from aerial parts of Lomatopodium staurophyllum (Rech.f.) Rech.f. was analyzed by capillary GC annd GC/MS. Among the 15 compounds identified, the major componeents were (E)-β-ocimene (26.8%%), myrcene (26.3%), (z)-β-ocimene (17.7%), ...
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The essential oil isolateed by water and steam distillation from aerial parts of Lomatopodium staurophyllum (Rech.f.) Rech.f. was analyzed by capillary GC annd GC/MS. Among the 15 compounds identified, the major componeents were (E)-β-ocimene (26.8%%), myrcene (26.3%), (z)-β-ocimene (17.7%), β-caaryophyllene (4.6%) and limonene (4.6%).
F. Sefidkon
Volume 10, Issue 1 , November 2001, , Pages 85-104
Abstract
The essential oils, water-steam distilled, from Foeniculum vulgare Mill., harvested at 3 ontogenic stages (inflorescence at flowering stage, unripe seed and ripe seed), were evaluated for their chemical composition by GC and GC-MS. Twenty-two compounds were identified and the main constituents were (E)- ...
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The essential oils, water-steam distilled, from Foeniculum vulgare Mill., harvested at 3 ontogenic stages (inflorescence at flowering stage, unripe seed and ripe seed), were evaluated for their chemical composition by GC and GC-MS. Twenty-two compounds were identified and the main constituents were (E)- anethole, limonene, fenchone and methyl chavicol. Higher oil yields were found at the unripe seed stage (5% w/w), but higher (E)- anethole percentage was found in the oil of ripe seed and chemical composition was influenced by the harvesting time.
