Extraction, Separation and Identification of flavonoides (Quercetine and Robinine) from Robinia pseudoacacia L.

Sefidkon, F.; Agha-Vali Jamaat, A.; Alinia Rodsari, M.; Jimand, K.

Document Type : Research Paper

Authors

¹ Research Institute of Forests and rangelands, P. O. Box: 13185-116, Tehran

² Zanjan University, Faculty of Science, Chemistry Department, Zanjan

Abstract

Robinia pseudoacacia (Black locust ) is an ornamental tree with beautiful flowers. It,s main distribution is North America which spreads to Europe , Asia and Iran. Today, it is naturalized in Iran and grows in every where.
Previously, we reported chemical composition of the essential oil and absolute of hexan extract of R. pseudoacacia.
In this research, the flavonoids from flowers and leaves of Robinia pseudoacacia were investigated. The extracts of flowers and leaves of R. pseudoacacia were obtained by Sochselet apparatus (by petroleum ether, benzen, chlorform, acetone and methanol) and also by macceration in methanol-water (9:1) and then methanol-water (1:1) and extraction with chlorform and hexan. After purification and separation of the extracts by P.C, HPLC, column and flash chromatography, two flavonoids, Quercetine and Robinine were obtained as pure compounds. Structure of the compounds were charachterized by HPLC (using standards), U.V. and ¹HNMR .

Keywords

References

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Iranian Journal of Medicinal and Aromatic Plants Research

Extraction, Separation and Identification of flavonoides (Quercetine and Robinine) from Robinia pseudoacacia L.

References

References

Volume 20, Issue 1 - Serial Number 23
April 2004
Pages 19-38

Extraction, Separation and Identification of flavonoides (Quercetine and Robinine) from Robinia pseudoacacia L.

References

References

Volume 20, Issue 1 - Serial Number 23April 2004Pages 19-38

Volume 20, Issue 1 - Serial Number 23
April 2004
Pages 19-38