Document Type : Research Paper
Authors
Research Institute of Forests and Rangelands, P.O. Box 13185 - 116, Tehran, Iran
Abstract
Melissa officinalis L. is a very easily grown plant that habitats in south and Eastern of Europe, Spain and Asia. The plant of Melissa collected from three fields in Fars, Tehran and Semnan provinces. Essential oils were isolated by hydro-distillation.
The yields of Essential oils of Fars sample (Shiraz), Tehran sample (Karaj) and Semnan sample were 0.14%, 0.25% and 0.26% (w/w) respectively. The oils were analyzed by GC (9A-Shimadzu) and GC/MS (Varian 3400). Seventeen constituents at Fars sample oil, seventeen constituents at Tehran sample oil and fourteen constituents at Semnan sample oil were identified respectively.
Major constituents of Fars sample were citronellal (25.4%), b-caryophyllene (11.3%), thymol (10.5%), globolol (6.1%), caryophyllene oxide (6.5%) and geraniol (5.7%). Major constituents of Karaj sample oil were citronellal (9.3%), carvacrol (31.8 %), Humulene epoxide (25.4 %) and a-humulene (10.2%). Major constituents of Semnan samples oil were citronellal (43.8%), b-caryophyllene (13.5%), thymol (7.9%), globolol (6.8%), neral (5.3%) and geranial (5.0%).
Fourteen constituents were common at all sample oil. Major constituent at all samples oil was citronellal. Four major constituents were common at Fars and Semnan samples oil.
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