Volume 30, Issue 5 , December 2014
Z. Ranjbar; F. Shariatmadari; M.A. Karimi Torshizi
Abstract
This experiment was designed to study the effects of feeding diets containing different levels of yucca extract and antibiotic on growth performance, blood parameters, immune system and ascites index. Three hundred and twelve, one-day old broiler chicks (Arian) were used in a completely randomized design ...
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This experiment was designed to study the effects of feeding diets containing different levels of yucca extract and antibiotic on growth performance, blood parameters, immune system and ascites index. Three hundred and twelve, one-day old broiler chicks (Arian) were used in a completely randomized design with four treatments, consisting of three replications and 26 (male & female) chicks per pen. The treatments consisted of control (without supplementation), Yucca schidigera extract at 150 and 225 mg/kg, and virginiamycin at 15 mg/kg up to six weeks of age. Significant differences were found for feed intake, feed efficiency, and body weight gain in the studied treatments (p < 0.01). Immune responses, expressed as a primary and secondary antibody response to SRBC, were affected by treatments (p < 0.01). Treatment groups did not affect the weights of organs (p < 0.05). Effects of treatments on cholesterol, triglycerides, HDL and LDL levels, at 35 days of age, were significant on the parameters evaluated in different experimental groups (p < 0.01). The trends observed indicate that Yucca schidigera extract could have beneficial effects on broiler chicks performance.
M. Khosravi; S.Gh. Moosavi; M.J. Seghatoleslami
Abstract
In order to study the effect of irrigation interval, Nitrogen (N) fertilization and plant density on yield and yield components of fenugreek (Trigonella foenum-graecum L.), a split-split-plot experiment was conducted in Darmian, Iran in Spring 2010 based on a randomized complete block design with three ...
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In order to study the effect of irrigation interval, Nitrogen (N) fertilization and plant density on yield and yield components of fenugreek (Trigonella foenum-graecum L.), a split-split-plot experiment was conducted in Darmian, Iran in Spring 2010 based on a randomized complete block design with three replications. The main plots were irrigation interval at two levels of 5 and 10 days. The sub-plots were N fertilization at three rates of 0, 75 and 150 kg N.ha-1 from urea source and the sub-sub-plots were plant density at three levels of 22, 33 and 66 plants.m-2. According to the results of analysis of variance, irrigation interval significantly affected traits of pod length and water use efficiency (WUE) for seed and biomass production. In addition, main branch number per plant, pod length, seed yield, chlorophyll index and WUE for seed and biomass production, were significantly affected by nitrogen rate and plant density had significant effect on main branch number per plant and ring diameter. Means comparison showed that as irrigation interval was increased from 5 to 10 days, pod length decreased 8.7%, but WUE for seed and biomass production increased 40.5 and 47.5%, respectively. Also, as N rate was increased from 0 to 150 kg N.ha-1, main branch number per plant, pod length, seed yield, chlorophyll index and WUE for seed and biomass production increased by 23.5, 26.4, 23.2, 67.1, 72.3 and 71.5%, respectively. Moreover, with increasing of density from 22 to 66 plant. m-2, branch number per plant and ring diameter decrased 7.9 and 11.2%, respectively. In total, given the results of the study and the importance of water saving, it is recommended to use irrigation interval of 10 days with the application of 150 kg N.ha-1 and density of 66 plants.m-2 for the cultivation of fenugreek in Darmian, Iran.
M. Ghanbari; M.K. Souri; R. Omidbaigi; H. Hadavand Mirzaei
Abstract
Achillea millefolium L. is a well-known species amongst the members of Achillea (Asteraceae) which grows naturally in Iran. In this study, the natural habitats of Achillea millefolium L.were identified in different regions of East Azerbaijan province during the growth season in 2011. The evaluation of ...
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Achillea millefolium L. is a well-known species amongst the members of Achillea (Asteraceae) which grows naturally in Iran. In this study, the natural habitats of Achillea millefolium L.were identified in different regions of East Azerbaijan province during the growth season in 2011. The evaluation of morphological variation in 35 genotypes of seven populations of Achillea millefolium L. was carried out to determine superior characters for future breeding programs and medicinal purposes. In this study, 14 quantitative and qualitative characters were evaluated. Flowering stems were used to investigate the quantity of essential oil. Data of locations, vegetative and reproductive characters of each population and the amount of essential oil obtained from each location were recorded. Results were analyzed using cluster analysis with SPSS software. Populations were clustered based on vegetative and reproductive characters and the amount of essential oil. The results of simple correlation analysis showed the existence of significant, positive and negative correlations among some important characters. Factor analysis was also used for defining the determinant factors. According to the factor analysis, flowering stem length, internode length and leaf width constituted the main factors. The populations of Achillea millefolium L. were separated into four groups by cluster analysis using Ward method. The most similar populations were Pirbala, Kondlaj and Jolfa, while Shabestar and Zonoz were separated from other populations. Also populations of Bonab and Basmenj were clustered as a separate group. In conclusion, in this study from all populations, Basmanj and Jolfa populations due to higher yield of essential oil as well as higher adaptability to region climatic conditions are recommended for further physiological and breeding studies.
L. Safaei; E. Sharifi Ashoorabadi; D. Afiuni; S. Davazdah Emami; A.A. Shoaii
Abstract
In order to study the effects of different methods of soil fertilization on thyme (Thymus daenensis Celak), a research was carried out during 2007- 2010 in Research Center for Agriculture and Natural Resources, Esfahan. The experiment was laid out as a split plot in a randomized complete block design ...
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In order to study the effects of different methods of soil fertilization on thyme (Thymus daenensis Celak), a research was carried out during 2007- 2010 in Research Center for Agriculture and Natural Resources, Esfahan. The experiment was laid out as a split plot in a randomized complete block design with three replications. The treatments were four fertilizing systems including NPK in three levels of N=100, P=50 and k=50, N=150, P=100 and k=100, N=200, P=150 and k=150 in conventional cropping systems, four levels of manure fertilizer (5, 15, 25 and 35 ton/ha) in organic method, and combination of chemical and manure fertilizers in four levels (N=80, P=64 and k=64 Kg/ha with 5 ton/ha manure fertilizer, N=60, P=48 and k=48 Kg/ha with 15 ton/ha manure fertilizer, N=40, P=32 and k=32 Kg/ha with 25 ton/ha manure fertilizer, N=20, P=16 and k=16 Kg/ha with 35 ton/ha manure fertilizer in intermediate nutrition management and control. Based on the results, shoot dry yield and oil yield in intermediate nutrition treatment were higher as compared to other treatments.Also the highest shoot dry yield and oil yield was obtained from the first harvesting stage. The first harvesting stage of intermediate nutrition treatment (combination of 35 ton/ha manure and NPK= 40- 32- 32 Kg/ha) in the second year, had the highest amount of shoot dry yield and oil yield (2597.78 and 81.63 kg/ha, respectively). Therefore, in comparison with control, shoot dry yield and oil yield increased by 112.73% and 163.32%, respectively. A positive and significant correlation was observed between shoot dry yield and oil yield, essential oil percentage and dry weight to wet weight ratio. Consequently, the combination of NPK and manure fertilizers could be recommended as a suitable nutrition source for plant as well as the improvement of soil structure.
A. Riahi-Madvar; K. Yousefi; M. Nasiri-Bezenjani
Abstract
Lemon balm(Mellissa offisinalis L.) belongs to the Lamiaceae family with medicinal properties such as anti-bacterial, anti-depressants, anti-inflammatory, anti-cancer and anti-viral effects. The most pharmacological effects of this plant are related to its active ingredient, rosmarinic acid. In this ...
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Lemon balm(Mellissa offisinalis L.) belongs to the Lamiaceae family with medicinal properties such as anti-bacterial, anti-depressants, anti-inflammatory, anti-cancer and anti-viral effects. The most pharmacological effects of this plant are related to its active ingredient, rosmarinic acid. In this research, the effects of different concentration of elicitors, yeast extract (0, 0.5, 1 and 2 mg/mL) and copper (0, 4, 8 and 16 μM) at different time intervals (4, 8 and 16 h) were investigated on rosmarinic acid and total flavonoid contents in 30-day-old seedlings of lemon balm greenhouse conditions. Results showed a significant effect of yeast extract on rosmarinic acid production, especially after 4 and 8 hours of treatment with this elicitor. The highest amount of this compound was observed in seedlings treated with 1 mg/mL of this elicitor. On the other hand, copper elicitor at all used concentrations and the duration time of treatment (except for 4 μM for 16 hours) significantly increased rosmarinic acid production, so that this elicitor at concentration of 8 µM for 8 hours treatment could induce rosmarinic acid level about 7-fold. It seems that the elevated level of this active ingredient under treatment with these elicitors is due to the induction of reactive oxygen radicals and jasmonic acid biosynthesis and subsequently expression of some genes involving in rosmaric acid biosynthesis.
Z. Habibi; R. Ghahremanzadeh; M. Yousefi
Abstract
The aerial parts of Salvia persepolitana Boiss. were collected during the flowering stage from IlamProvince. Purification of chloroform extract of S. persepolitana was carried out by column chromatography on silica gel with a gradient of n-hexan/ethyl acetate. At the end of chromatography, the column ...
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The aerial parts of Salvia persepolitana Boiss. were collected during the flowering stage from IlamProvince. Purification of chloroform extract of S. persepolitana was carried out by column chromatography on silica gel with a gradient of n-hexan/ethyl acetate. At the end of chromatography, the column was eluted by methanol. Further purification was carried out by column chromatography (smaller columns) and thin layer chromatography (glass plates) in n-hexane–ethyl acetate as solvent for several times and yielded pure compounds. The structure of purified natural products was elucidated by using Nuclear Magnetic Resonance (NMR) spectroscopy and Mass spectrometry. The chloroform extract of S. persepolitana yielded two known diterpenoids as manool and sclareol and one known flavonoid namely ladanein. The amount of purified sclareol was 3% (18g) of the total plant weight, so S. persepolitana can be a good sourse for sclareol.
F. Najafi; S. Mehrabian; R. Khavari-Nejad; Y. Ghorbani
Abstract
In the present study, the effects of different concentrations of NaCl (0, 15, 30, 45, 60, 75 and 100mM) were investigated on some physiological parameters and antibacterial activities of dill (Anethum graveolens L.). Ten-days-old seedlings, prepared in sterilized petri dishes, were transferred into pots ...
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In the present study, the effects of different concentrations of NaCl (0, 15, 30, 45, 60, 75 and 100mM) were investigated on some physiological parameters and antibacterial activities of dill (Anethum graveolens L.). Ten-days-old seedlings, prepared in sterilized petri dishes, were transferred into pots containing washed sand and irrigated with Hoagland nutrient solution. Pots were located in a growth chamber (at 25°C for 16h in light and 18°C for 8h in darkness) and after certain growth period, plants were harvested for analyses. For antibacterial analyses, the plants were treated throughout flowering and fruit production stages. Results showed that in all treated plants, proline content was increased and photosynthetic pigments were decreased in high concentrations of NaCl. Also, the studies on growth factors showed that in dill, these parameters were affected by NaCl. Studies on antibacterial activities showed that in most of the cases, the zone of inhibition was increased by increasing of salt concentration and the antibacterial effect of extract was significant in high concentrations. Of course, choosing the best way and solute for preparing the extract is important for reaching a noticeable antibacterial effect.
H. Batooli; A. Haghir Ebrahimabadi; B. Mahmodi; A. Mazochi
Abstract
Dorema D. Don. genus belongs to the Umbelliferae family with important medicinal and aromatic species, containing more than 16 species worldwide, of which 6 species have been reported in Iran. In this research, the essential oil composition of vegetative and reproductive organs of Dorema ammoniacum D. ...
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Dorema D. Don. genus belongs to the Umbelliferae family with important medicinal and aromatic species, containing more than 16 species worldwide, of which 6 species have been reported in Iran. In this research, the essential oil composition of vegetative and reproductive organs of Dorema ammoniacum D. Don. obtained from Shahsavaran, Kashan was investigated. This species is endemic to semi-arid regions of Iran and Afghanistan, growing naturally on rocky and alluvial lands. The stem, leaf, fruit and root of this species were collected in spring 2011 and dried in shade (at room temperature). The essential oil was extracted by simultaneous steam distillation and solvent extraction (SDE). The chemical composition of the essential oil was analyzed by using GC and GC-MS. According to the obtained results, 18, 13, 12 and 12 components were identified in the essential oil of dry stem and leaf; fresh stem and leaf; fruits, and roots respectively of which the major components were as follows: (E)-nerolidol (11.7%), E, E-farnezyl acetone (8.9%), phytol (8.9%), di-n-butyl phthalate (6.9%), 2(E)-tridecen-1-al (6.1%) and α-farnesol( 5.5%) in the essential oil of dry stem and leaf; phytol (15.2%), (E)-nerolidol (14.6%), α-farnesol (14%), 2-(E)-tridecenol (12.8%) and tetradecanal (5.6%) in the essential oil of fresh stem and leaf; α-farnesol (41.2%), β-bisabolene (13%), α-bisabolol (6.4%), and (Z)-farnesal (5.5%) in the essential oil of fruits, and β-bisabolene (56.1%), elemicin (12.2%) and tridecanol (6.1%), in the essential oil of roots. Sesquiterpenes constituted the main chemical group in the essential oil obtained from the roots and fruts of Dorema ammoniacum in Shahsavaran, Kashan.
F. Askari; E. Sharifi Ashorabadi; M. Mirza; M. Teimouri; E. Ehsani
Abstract
This research was aimed to investigate the effect of collection locality and essential oil concentration of Thymus pubescens Boiss. & Kotschy ex Celak on antimicrobial activity against some microorganisms. The statistical design used in this study was a factorial experiment in a completely randomized ...
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This research was aimed to investigate the effect of collection locality and essential oil concentration of Thymus pubescens Boiss. & Kotschy ex Celak on antimicrobial activity against some microorganisms. The statistical design used in this study was a factorial experiment in a completely randomized design with four replications. In this experiment, collection locality at six levels including the habitats of West Azarbaijan, Tehran, Zanjan, Qazvin, Kurdistan and Guilan, essential oil dilution at three levels including one fifth (1:5), one twenty-fifth (1:25), one fiftieth (1:50) and comparison with the antibiotics of ciprofloxacin and ceftizoxime, and studied microorganisms at five levels including Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeroginosa and Candida albicans were investigated. The seeds were collected from different habitats and cultivated in the field of Research Institute of Forests and Rangelands. The essential oil was isolated from shoots by hydro-distillation. Chemical compositions of the oils were analyzed by GC and GC/MS. The essential oil yield obtained from different localities varied between 0.39% to 0.83% (w/w). The major constituents of the essential oil obtained from the samples collected from West Azarbaijan, Tehran, Zanjan, Qazvin, Kurdistan and Guilan were as follows: West Azarbaijan: E-caryophyllene (26.0%) and camphor (24.2%); Tehran: geranial (30.9%) and geranyl acetate (23.9%); Zanjan: linalool (23.5%) and 1,8-cineol (22.2%); Qazvin: thymol (30.3%) and carvacrol (30.1%); Kurdistan: linalool (17.2%) and geranyl acetate (12.7%), and Guilan: α–terpineol (31.2%) and geraniol (11.2%). Analysis of variance of inhibition zone diameter of T. pubescence essential oil showed significant difference (p<0.05) among collection locality, microorganism type, essential oil dilution and their interaction. Mean comparisons of the collection locality, microorganism type and essential oil dilution showed that the highest inhibition zone diameter (35.5mm) was recorded for the essential oil obtained from West Azarbaijan, ciprofloxacin against Bacillus subtilis. The lowest inhibition zone diameter (8.0 mm) was observed in the essential oil obtained from West Azerbaijan with essential oil dilution of 1:50 against P. aeruginosa. According to the MIC and MBC results, the most and the least antimicrobial activity was recorded for the essential oils obtained from the seeds collected from Tehran and Kurdistan, respectively.
H.R. Sarraf Moayeri; F. Pirayeshfar
Abstract
The two-spotted spider mite, Tetranychus urticaeKoch, is one of the most important and polyphagous pests which damages a wide range of agricultural crops throughout the world. In recent decades, excessive conventional acaricide application leads to the development of pest resistance and environmental ...
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The two-spotted spider mite, Tetranychus urticaeKoch, is one of the most important and polyphagous pests which damages a wide range of agricultural crops throughout the world. In recent decades, excessive conventional acaricide application leads to the development of pest resistance and environmental pollutions. In recent years, herbal essential oils have received attention as natural plant protection compounds. In this study, the fumigant toxicity of three medicinal plant essential oils including shirazi thyme (Zataria multiflora Boiss.), galbanum (Ferula gummosa L.) and savory (Satureja hortensis L.) were tested against the eggs and female adultsof T. urticae. The essential oils were obtained by Clevenger-type water distillation. Experiments were carried out at 25±1°C, 50±5% RH and under a photoperiod of 16L: 8D. The median lethal concentration (LC50) for shirazi thyme, savory and galbanum essential oils were recorded to be 21.962, 30.894 and 56.783 µl/L air, 24h after treatment, respectively. In ovicidal bioassays, the LC50 values for oils were 17.493, 43.07 and 101.61 µl/L air, respectively. According to the obtained results, the highest lethal effects were obtained for shirazi thyme oil against the eggs and adults of T. urticae as compared to the other essential oils.
S. Kamali; M. Elahi; M. Hosseini Nejadand; M. Yavarmanesh
Abstract
Because of various technological properties, beneficial prebiotic and health effects, inulin is extensively used in different products and symbiotic combinations. The survival of probiotic strains during gastric stress is influenced by the physicochemical properties of food carriers used for delivery. ...
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Because of various technological properties, beneficial prebiotic and health effects, inulin is extensively used in different products and symbiotic combinations. The survival of probiotic strains during gastric stress is influenced by the physicochemical properties of food carriers used for delivery. In this study, the possibility of increasing the growth and survival potential of two Lactobacillus strains (Lactobacillus casei PTCC 1608 and Lactobacillus rhamnosus PTCC 1637) was investigated in the presence of inulin, extracted from different plant sources (Cichorium intybus & Heliantus tuberosus), and standard inulin under acidic conditions (pH= 4, 2.5) and pH=6.2 as control and compared to glucose. Our results clealy showed that the addition of carbohydrate to lactobacillus cultures significantly increased the growth and resistance of bacteria under acidic conditions. The inulin extracted from Cichorium intybus and standard inulin were more effective in increasing the resistance of bacteria as compared to the inulin extracted from Heliantus tuberosus.
F. Bagheri; M. Mohammadi Sharif; A.R. Hadizadeh
Abstract
In the current study, the influence of time factors was investigated on application of Eucalyptus globulus Labill., essential oil against the adults of confused flour beetle Tribolium confusum Jacquelin du Val. The containers were treated with 205, 328, 460, 615 and 902 µl/L air of the essential ...
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In the current study, the influence of time factors was investigated on application of Eucalyptus globulus Labill., essential oil against the adults of confused flour beetle Tribolium confusum Jacquelin du Val. The containers were treated with 205, 328, 460, 615 and 902 µl/L air of the essential oil and the adults were exposed 1, 2, 4, 8, 16 and 24 hours after treatment, for evaluating the effectiveness period of the essential oil. LC50 values were estimated in other experiments based on 1, 2, 4, 8 and 16 hours exposure times. Finally, the cumulative mortalities of the essential oil were recorded at 3, 6, 9, 12 and 24 hours after treatment. According to the obtained results, a delay more than 16 hours reduced the efficacy of the essential oil to the lowest level (0% mortality) in first assays. LC50 values of the second bioassays were 1384, 201.2, 131, 130.2 and 109 µl/L air, respectively. These values suggested that keeping closed the treating area as long as four hours was sufficient to access effective results. Gradual increasing of mortality was observed in cumulative assays and mortalities at concentrations of 90, 170, 340, 510, 680 and 850 µl/L air after 24 hours were calculated to be 41.6, 91.6, 100, 100, 100 and 100%, respectively. The last results demonstrated that the most proportion of mortality occured within the first three hours after treatment. Therefore, by considering the quick mortalities caused by the essential oil and rapid decreasing of its efficacy, the essential oil application time must be accurately selected to acquire its maximum efficiency.
M. Moosavy; N. Shavisi
Abstract
Listeria monocytogenes is an important food-borne pathogen and its control in foods is a significant challenge. This study evaluated the effectiveness of Mentha spicata essential oil on limitingL. monocytogenes growth at different temperatures (4, 9 and 14°C), pH (5, 6 and 7) and NaCl concentrations ...
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Listeria monocytogenes is an important food-borne pathogen and its control in foods is a significant challenge. This study evaluated the effectiveness of Mentha spicata essential oil on limitingL. monocytogenes growth at different temperatures (4, 9 and 14°C), pH (5, 6 and 7) and NaCl concentrations (0, 1, 2 and 4 g/100mL). Chemical constituents of the M. spicata essential oil were identified by gas chromatography/mass spectrometry (GC/MS). The susceptibility of bacterium was performed by the minimal inhibitory concentration (MIC) using the micro-broth dilution technique. The major detected components were carvone (78.76%), limonene (11.50%) and menthol (1%). The MIC value of essential oil was 160µl/mL. The results obtained showed that the effectiveness of M. spicata was pronounced by increasing of salt and temperature and decreasing pH. In conclusion, the encouraging results indicated that the essential oil of M. spicata might be exploited as natural preservative for the control of L. monocytogenes.
F. Shekari; A. Mehrafarin; H.A. Naghdi Badi; R. Hajiaghaei
Abstract
The use of bio-stimulators is very important in getting better quality and plant performance sustainability. Therefore, a study was conducted on Plantago psyllium L. at research field of the Institute of Medicinal Plants, ACECR in 2011 on the basis of Complete Randomized Blocks Design with 10 treatments ...
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The use of bio-stimulators is very important in getting better quality and plant performance sustainability. Therefore, a study was conducted on Plantago psyllium L. at research field of the Institute of Medicinal Plants, ACECR in 2011 on the basis of Complete Randomized Blocks Design with 10 treatments and three replications. The treatments were included bio-stimulators including Kadostim, Fosnutren, Humiforte and Aminolforte in two levels of 0.75 and 1.5 L.ha-1, chemical fertilizer (70 kg.ha-1) and control (without bio-stimulators and fertilizer). Results showed that the treatments had significant effect on number of axillary shoots, number of spikes per plant, number of seeds per plant, 1000- grain weight and seed yield (p<0.01), plant height, spike width, number of seeds per spike and shoot dry yield (p<0.05). However, the study treatments had no significant effect on spike length. The maximum plant height, number of axilliary shoots and shoot dry yield were obtained in Kadostim 1.5 L.ha-1. The maximum spike width and number of seeds per spike were achieved by Fosnutren 1.5 L.ha-1. The highest 1000-grain weight was recorded in treatments of Kadostim and Fosnutren 1.5 L.ha-1 and the maximum number of spikes per plant and seeds per spike were observed in treatment of chemical fertilizer. The maximum and minimum seed yield (445 and 149 kg.ha-1, respectively) was achieved by treatment of Fosnutren 0.75 L.ha-1 and Humiforte 0.75 L.ha-1, respectively. Our results clearly showed that the consumption of chemical fertilizers could be reduced by using bio-stimulators as an attempt towards sustainable agriculture and reducing environmental pollution.
S.M. Mehdizadeh; A. Zarei; H. Lotfollahin; F. Mirzaei; M.H. Lebaschi; M. Haji Mohammadi
Abstract
This study was carried out using a completely randomized design to evaluate the effects of different levels of dietary inclusions of Aleo vera gel, probiotics and antibiotic on performance, immune response and egg qualitative traits in 288 commercial laying hens (Hy – line) at the age of 29 weeks ...
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This study was carried out using a completely randomized design to evaluate the effects of different levels of dietary inclusions of Aleo vera gel, probiotics and antibiotic on performance, immune response and egg qualitative traits in 288 commercial laying hens (Hy – line) at the age of 29 weeks for 84 days. Hens were divided into six treatments and 4 replicates with 12 birds each and were offered standard feed ration with free water. The treatments were as follows: T1= Control groups (without feed additive), T2= T1+ 80mg antibiotics/kg feed, T3= T1+ Aleovera gel 1.5%/kg feed, T4= T1+ Aleovera gel 3%/kg feed, T5=T1+ probiotics 0.05%/kg feed and T6= T1+ probiotics 0.1%/kg feed. Results showed that, Aleovera gel had significant effects on egg production, feed efficiency and egg mass as compared to control groups (p<0.05), but the highest feed efficiency was belonged to probiotics treatments compared to the rest (p<0.05). No significant difference was observed in egg qualitative traits like hugh unit, yolk coloration, egg specific gravity, and egg shell strength between experimental diets and control group (p<0.05). Experimental diet containing 1% probiotic caused a reduction in blood cholesterol as compared to other experimental groups (p<0.05). Diet supplemented by inclusions of probiotic at 0.1%/kg feed had significant effect on IgM and IgG level, antibody titre against Newcastle disease virus and antibody titre against sheep red blood cell (p<0.05). Finally, probiotic treatments performed well with excellent results on antibody titre against sheep red blood cell as compared to control and other related treatments throughout the experimental period (p<0.05). Aleo vera treatments performed well with excellent results on antibody titre against Newcastle disease virus as compared to control and other related treatments throughout the experimental period (p<0.05). Therefore, it is concluded that the application of Aleo Vera product could improve the performance of the laying hens and consequently increases the economic profit.
K. Saeidi; F. Sefidkon; A. Babaei
Abstract
Dog-rose fruit has valuable secondary metabolites such as carotenoids. This research was aimed to determine the carotenoids and lycopene content of dog-rose (Rosa canina L.) fruit in different habitats and awareness of the diversity of these compounds. For this purpose, the fruits were collected from ...
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Dog-rose fruit has valuable secondary metabolites such as carotenoids. This research was aimed to determine the carotenoids and lycopene content of dog-rose (Rosa canina L.) fruit in different habitats and awareness of the diversity of these compounds. For this purpose, the fruits were collected from 60 regions of the country during 2010-2012. Carotenoids and lycopene content were measured by spectrophotometer at wavelengths of 450 and 503 nm. Total carotenoids varied from 0.195 to 1.097 ma/g Fw. The highest and lowest total carotenoids were obtained from IR34 and IR9 accessions, respectively. Lycopene ranged between 0.029-0.32 mg/g FW and the highest and lowest lycopene was obtained from IR45 and IR9 accessions, respectively. Carotenoids and lycopene content showed significant differences in the studied regions (p < 0.05). According to the results of correlation, a significant positive correlation was found between total carotenoids and lycopene (r=0.726). In our study, the studied accessions were classified into three different clusters (C1-C3). Results from this study could be used in the future breeding program of dog-rose.
