Iranian Journal of Medicinal and Aromatic Plants Research

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Investigation of genetic and phytochemical diversity of some catnip (Nepeta cataria L.) populations by RAPD molecular marker and GC/MS method

Document Type : Research Paper

Authors

1 Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran

2 M.Sc. student of Agriculture Biotechnology, Faculty of Agriculture, Payam Noor University, Tehran, Iran

3 Department of Agriculture Biotechnology, Faculty of Agriculture, Payam Noor University, Tehran, Iran

Abstract

Catnip (Nepeta cataria L.), also called catmint, is a medicinal plant containing aromatic compounds and belongs to the Lamiaceae family. In this study, phytochemical and genetic variations of some Catnip germplasm were analyzed using RAPD molecular marker as well as GC/MS. The shoots of plants were collected from various habitats and dried away from direct sunlight. DNA was extracted using (CTAB) method. Eleven RAPD primers were used in PCR reaction. Based on the results of cluster analysis, 15 populations were classified into four groups. Based on the results of principal component analysis, the plant populations were clustered into five groups. Eight of 15 collected populations were analyzed phytochemically. Essential oils were extracted via water steam distillation using Clevenger apparatus. Twenty-seven components were identified from all essential oil samples. The results of GC/MS method showed that among the samples, 100% of essential oil compounds were identified in populations of Sirch, Mohammad Abad-e-Maskoon, Saqder 2, Dalfard 2, and Mijan 2. The results also showed that in samples of Dehbakri, Dalfard 1, and Saqder 1, 94.84%, 99.8% and 96.6% of essential oil compounds were identified, respectively. Three isomers of Nepetalactone constituted the major essential oils compounds. B-caryophyllene, Caryophyllene oxide, β-pinene, and α-pinene were also of main essential oil compounds. Based on the cluster analysis of GC/MS data, the populations were classified into three groups. Comparison of consisting compounds of essential oils in the eight studied populations indicated that the essential oils extracted were different quantitatively and qualitatively. This could be probably due to the different ecological conditions of habitats such as humidity, temperature, altitude above sea level, or other terrestrial, geographical and genetic factors.

Keywords

References
- Adams, R.P., 2004. Identification of essential oil components by gas chromatography /quadrupole mass spectroscopy. Journal of the American Society for Mass Spectrometry, 16(11): 1902-1903.
- Akhgar, M.R. and Moradalizadeh, M., 2012. Chemical composition of the essential oils from stems, flowers and leaves of Nepeta schiraziana Boiss. Iranian Journal of Medicinal and Aromatic Plants, 28(1): 28-34.
- Ali, T., Javan, M., Sonboli, A. and Semnanian, S., 2012. Evaluation of the antinociceptive and antiinflammatory effects of the essential oil of Nepeta pogonosperma Jamzad et Assadi in rats. DARU Journal of Pharmaceutical Sciences, 20(1): 48.
- Batooli, H. and Safaei-Ghomi, J., 2012. Comparison of essential oil composition of three Nepeta L. species from Kashan. Iranian Journal of Medicinal and Aromatic Plants, 28(1):161-175.
- Dalir, M., 2011. Evaluation of genetic, morphology and chemical diversity Thymus spp. Thesis for M.Sc., Payame Noor University of Tehran.
- Davies, N.W., 1990. Gas chromatographic retention indices of monoterpenes and sesquiterpenes on methyl silicone and Carbowax 20M phases. Journal of Chromatography, 503: 1-24.
- Fakhr Ranjbar, H., 2010. Viability study of section cataria of genus Nepeta in Iran, Thesis for M.Sc., Tarbiyat Moalem University of Tehran.
- Farsi, M. and Zol`ali, J., 2003. Principles of Plant Biotechnology. Publication of Ferdosi University, 495p.
- Ghannadi, A., Aghazari, F., Mehrabani, M., Mohagheghzadeh, A. and Mehregan, I., 2003. Quantity and composition of the SDE prepared essential oil of Nepeta macrosiphon Boiss. Iranian Journal of Pharmaceutical Research, 2(2): 103-105.
- Hadian, J., Nejad Ebrahimi, S., Mirjalili, M., Azizi, A., Ranjbar, H. and Friedt, W., 2011. Chemical and genetic diversity of Zataria multiflora Boiss. accessions growing wild in Iran. Chemistry & Biodiversity, 8(1): 176-188.
- Haji Rezai, M., Baghizadeh, A., Javadi, Gh. and Sadeghizadeh, M., 2008. Evaluation of the genetic diversity of Kerman pistachio based on RAPD molecular markers. Iranian Journal of Biology, 22(3): 462-469.
- Hatari, Z. and Zamani, Z., 2012. Evaluation genetic biodiversity Ziziphora teneuior L. of Iran by RAPD markers. Iranian Jornal of Horticultural Science, 43(3): 80-84.
- Heuskin, S., Godin, B., Leroy, P., Capella, Q., Wathelet, J. and Verheggen, F., 2009. Fast gas chromatography characterization of purified semiochemicals from essential oils of Matricaria chamomilla L. (Asteraceae) and Nepeta cataria L. (Lamiaceae). Journal of Chromatography, 1216(14): 2768-2775.
- Jamzad, Z., 2012. Colorful Flora of Iran (Labiateae Family). Publication of Research Institute of Forests and Rangelands and University of Tehran.
- Kumar, L.S., 1999. DNA markers in plant improvement: An overview. Biotechnology Advanced, 17: 143-182.
- Liu, J., Wang, L., Geng, Y., Wang, Q., Luo, L. and Zhong, Y., 2006. Genetic diversity and population structure of Lamiophlomis rotata (Lamiaceae), an endemic species of Qinghai-Tibet Plateau. Genetica, 128: 385-394.
- Mojab, F., Nickavar, B. and Hooshdar Tehrani, H., 2009. Essential oil analysis of Nepeta crispa and N. menthoides from Iran. Iranian Journal of Pharmaceutical Sciences, 5(1): 43-46.
- Nedjalka, V., 1996. Constituents of essential oils from Nepeta cataria L., N. grandihora. Journal of Essential Oil Research, 8: 639-643.
- Paterson, A.A., Londer, E. and Hewitt, I.D., 1988. Resolution of quantitative traits into Mendelian factors by using a complete linkage map of restriction fragment length polymorphisms. Nature, 335: 721-726.
- Pezhmanmehr, M., Hassani, M.S., Fakhre Tabatabaie, M. and Hadian, J., 2010. Evaluation of genetic diversity and differentiation of some Bunium persicum (Boiss.) populations using RAPD markers. Environmental Sciences, 7(2): 63-76.
- Rechinger, K.H., 1982. Labiatae. In Flora Iranica. No. 150, Akademische Druck-u. Verlagsanstalt, Graz, Austria, 590p.
- Sajjadi, S.E. and Mehregan, I., 2005. Chemical constituents of essential oil of Nepeta daenensis Boiss. Journal of Essential Oil Research, 17(5): 563-564.
- Zargari, A., 1990. Medicinal Plants (Vol. 4). Tehran University Publications, Tehran, 923.
- Zhu, J.J., Berkebile, Dennis R., Dunlap, C.E., Zhang, A., Boxler, D., Tangtrakulwanich, K., Behle, R., Baxendale, Frederick P. and BrewerGary, J., 2012. Nepetalactones from essential oil of Nepeta cataria represent a stable fly feeding and oviposition repellent. Faculty Publications: Department of Entomology, Paper 340.
Iranian Journal of Medicinal and Aromatic Plants Research
Volume 34, Issue 5 - Serial Number 91
November and December 2018
Pages 836-848
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