In collaboration with Scientific Association of Iranian Medicinal Plants

Document Type : Research Paper

Authors

1 M.Sc. of Phytochemistry, Department of Chemistry, Faculty of Science, University of Birjand, Birjand, Iran

2 Department of Chemistry, Faculty of Science, University of Birjand, Birjand, Iran

Abstract

Chavil (Ferulago angulata (Schlecht.) Boiss.), belonging to the apiaceae family, consists of 35 species in the world. While seven species exist in Iran, three species including Ferulago phialocarpa, Ferulago contracta Boiss., and Ferulago angulata are native to Khuzestan province. The present research was conducted to determine the composition and content of fatty acids, phenolic compounds, flavonoid, tannin and antioxidant in Ferulago angulata native to Izeh region, Khuzestan province. In this research, different organs of the plant were collected in may 2016 and dried under certain conditions. The measurement of compounds in the seeds was performed. The content of phenol and total flavonoid in the methanol, ethanol, and water extracts was measured with spectrophotometric method and the results were expressed as mg of gallic acid per mg of dry matter. The total tannin content of samples was determined using tannic acid as standard spectrophotometrically. Furthermore, the antioxidant activity of plant extracts was evaluated by inhibiting free radical 2, 2'-diphenyl-1-picrylhydrazyl (DPPH). Oil  extraction  from  seeds was  carried  out  by Soxhlet apparatus  using  n-hexane  as  solvent, and the GC-Mass method was used to identify the compounds in the plant oil. According to the results of this research, carried out on the seeds of this plant for the first time, it was determined that oleic acid (63.6%) and linoleic acid (19.7%) had the highest content of fatty acid and the lowest content of fatty acid content was related to myristic acid and heptadecanoic acid (0.1%).

Keywords

- Abdelaal Selim, Y. and Hassan Ouf, N., 2012. Anti-inflammatory new coumarin from the Ammi majus L. Organicand Medicinal Chemistry Letters, 2: 1-4.
- Amirghofran, Z., Bahmani, M., Azadmehr, A. and Javidnia, K., 2006. Anticancer effects of various Iranian native medicinal plants on human tumor cell lines. Neoplasma, 53: 428-433.
- Baser, H.C., Demirci, B., Demirci, F., Hashimoto, T., Yoshinori, A. and Yoshiaki, N.A., 2002. Ferulagone: A new monoterpene ester from Ferulago thirkeana essential oil. Planta Medica, 68(6): 564-567.
- Bousselsela, H., Benhouda, A., Yahia, M., Benbia, S., Ghecham, A. and Zidani, A., 2012. In vitro evaluation of antioxidant and antibacterial activites of extracts of Hertia cheirifolias leaves. Nutural Science, 4(11): 825-831.
- Demetzos, C., Perdetzoglou, D., Gazouli, M., Tan, K. and Economakis, C., 2000. Chemical analysis and antimicrobial studies on three species of Ferulago from Greece. Planta Medica, 66: 560-563.
- Elmasta, M., Drirtas, I., Isildak, O. and Aboul-Enein, H.Y., 2006. Antioxidant activity of S-Carone isolated from Spearmint (Mentha Spicata L.). Journal of Liquid Chromatography & Related Technologies, 29(10): 1465-1475.
- Fernandez-Agullo, A., Pereira, E., Freire, M.S., Valentao, P., Andrade, P.B., Gonzalez Alvarez, J. and Pereira, J.A., 2013. Influence of solvent on the antioxidant and antimicrobial properties of walnut (Juglans regia L.) green husk extracts. Industrial Crops and Products, 42: 126-132.
- Fukumoto, L.R. and Mazza, G., 2000. Assessing antioxidant and prooxidant activities of phenolic compounds. Journal of Agricultural and Food Chemistry, 48(8): 3597-3604.
- Guerrero, J.L.G., Guirado, C.M., Fuentes, M.M.R. and Pérez, A.C., 2006. Nutrient composition and antioxidant activity of 10 pepper (Capsicum annuun) varieties. European Food Research and Technology, 224: 1-9.
- Jimenez, B., Grande, M.C., Anaya, J., Torres, P. and Grande, M., 2000. Coumarins from Ferulago capillaris and F. brachyloba. Phytochemistry, 53: 1025-1031.
- Kaur, R., Kapoor, K. and Kaur, H., 2011. Plants as a source of anticancer agents. Journal of Natural Product and Plant Resources, 1: 119-124.
- Khalighi-Sigaroodi, F., Hadjiakhoondi, A., Shafiee, A., Mozaffarian, V.A., Shahverdi, A.R. and Alavi, S.H.R., 2006. Phytochemical analysis of Ferulago bernardii Tomk & M.Pimen. DARU Journal of Pharmaceutical Sciences, 14(4): 214-221.
- Khanahmadi, M., Rezazade, Sh., Shahrezaei, F. and Taran, M., 2009. Study on chemical composition of essential oil and anti-oxidant and anti-microbial properties of Artemisia haussknechtii. Journal of Medicinal Plants, 3(31): 132-141.
- Makkar, H.P.S., Bluemmel, M., Borowy, N.K. and Becker, K., 1993. Total phenolic, flavonoids and tannin contents in different extracts of Artemisia absinthium. Journal of the Science of Food and Agriculture, 61: 161-165.
- Mohammadi, A., Amiri, H., Khodayari, H., Zarei, A., Eghbali, D. and Azarbani, F., 2015. Phytochemical and anti-Candida evaluation of essential oil of Thymus eriocalyx (Ronniger) Jalas. In different stages of plant growth in Lorestan province. Eco-phytochemistry of medicinal plants, 3(2): 8-17.
- Naseri, M., Monsef-Esfahani, H.R., Saeidnia, S., Dastan, D. and Gohari, A.R., 2013. Antioxidant coumarins from rotts of Ferulago subvelutina. Asian Journal of chemistry, 25: 1875-1878.
- Omidbaigi, R., 2009. Production and Processing Of Medicinal Plants (Vol. 1). Astan Quds Razavi, 438p.
- Ozturk, B., Gur, S., Coskun, M., Kosan, M., Erdurak, C., Hafez, G., Ozgunes, O. and Cetinkaya, M., 2004. Relaxant effect of Ferulago syriaca root extract on human corpus cavernosum. European Urology Supplements, 3: 62.
- Rhazi, N., Hannache, H., Oumam, M., Sesbou, A., Charrier, B., Pizzi, A. and Bouhtoury, F.Ch., 2015. Green extraction process of tannins obtained from Moroccan Acacia mollissima barks by microwave: Modeling and optimization of the process using the response surface methodology RSM. Arabian Journal of Chemistry, In press. https://www.sciencedirect.com/science/article/pii/S1878535215001343.
- Rustaiyan, A. and Sedagat, S., 2002. Ferulago angulata (Schlecht.) Boiss. From Iran. Journal ofEssential Oil Research, 14(6): 447-448.
- Rustaiyan, A., Sedaghat, S., Larijani, K., Khossravi, M. and Masoudi, S., 2002. Composition of the essential oil of Ferulago angilata (Schlecht.) Boiss. Essential Oil Research, 14: 447-448.
- Taran, M., Ghasempour, H.R. and Shirinpour, E., 2010. Antimicrobial activity of essential oils of Ferulago angulata subsp. carduchorum. Jundishapur Journal of Microbiology, 3(1): 10-14.
- Verma, L., Singour, P.K., Chaurasiya, P.K., Rajak, H., Pawar, R.S. and Patil, U.K., 2010. Effect of ethanolic extract of Cassi occidentalis Linn. for the management of alloxan-ind uced diabetic rats. Pharmacognosy Research, 2: 132-137.