In collaboration with Scientific Association of Iranian Medicinal Plants

Document Type : Research Paper

Authors

1 M.Sc., Department of Biotechnology, Faculty of Agriculture, Bu Ali Sina University, Hamadan, Iran

2 Department of Biotechnology, Faculty of Agriculture, Bu Ali Sina University, Hamadan, Iran

3 Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

Abstract

Previous studies have shown an anti-tumoral effect for different species of plant having similar component with Elaeagnus angustifolia L.and Nepeta crispa L. on cancer cells. This study was performed to evaluate the anti-tumural effects of Elagnus angustifolia and Nepeta crispa extracts on leukemia cancer cells. Cancer cells, grown in DMEM/F12 culture medium, were supplemented with fetal calf serum, 100U/ml penicillin and 100μg/ml streptomycint, then incubation at 37°C in a 5% CO2. The fractions of Hexane, ethyl acetate, chloroform and aquesse at 10-160 μg/ml concentration were applied on K562 leukemic cells, and their effects were evaluated on proliferation and viability of cells by cell counter and LDH kits, respectively. Result showed that chloroform (chf), ethylacetate (EA) fractions of Elaeagnus angustifolia and Nepeta crispa showed an important cytotoxic effect on K562 cells. Isolated extracts from E. angustifolia flower and N. crispa caused a significant decrease in lukemia cancer cell growth. Therefore, this plant could be candidate for therapeutic or preventive activity against cancer-related disorders. Isolation and purification of effective compound/s from this extract and determination of their mechanisms of action are suggested.

Keywords

- Abu-Dahab, R. and Afifi, F., 2007. Antiproliferative activity of selected medicinal plants of Jordan against a breast adenocarcinoma cell line (MCF7). Sciential Pharmaceutical, 75: 121-136.
- Calixto, J.B., 2000. Efficacy, safety, quality control, marketing and regulatory guidelines for herbal medicines phytotherapeutic agents. Brazilian Journal and Biological Research, 33: 179-189.
- Eikesdal, H.P., Bjerkvig, R., Mella, O. and Dahl, O., 2001. Combretastatin A-4 and hyperthermia; a potent combination for the treatment of solid tumors. Radiotherapy and Oncology, 60(2): 147-154.
- Emami, A., Zamanitaghizadeh Arba, Sh., Ahi, A. and Mahmoudi, M., 2010. The inhibitory effect of Artemisia annua extracts on gastric cancer cells via apoptosis induction. Journal of Shahrekord University of Medical Sciences, 11(4): 1-11.
- Falson, G., Glea, A.E. and Noack, E.A., 1982. Constituents of Euphorbiaceae, 7.20-deoxyingenol monoesters and ingenol diesters from biglandulosa. Archive of Pharmacology Journal, 315(12): 1026-1032.
- Hosseinzadeh, H., Ramezani, M. and Namjo, N., 2003. Muscle relaxant activity of Elaeagnus angustifolia L. fruit seeds in mice. Journal of Ethnopharmacology, 84(2-3): 275-278.
- Hoyert, D.L., Kung, H.C. and Smith, B.L., 2003. Deaths: Preliminary Data for 2003. National Vital Statistics Reports, 53(15): 1-4.
- Li, L.H., Baek, I.K., Kim, J.H., Kang, K.H., Koh, Y.S., Jung, Y.D., Cho, C.K., Choi, S.Y. and Shin, B.A., 2009. Methanol extract of Elaeagnus glabra, a Korean medicinal plant, inhibits HT1080 tumor cell invasion. Oncology Reports, 21(2): 559-563.
- Li, Y., Fang, H. and Xu, W., 2007. Recent advance in the research of flavonoids as anticancer agents. Mini Reviews of Medicinal Chemistry, 7(7): 663-678.
- Mattos, D., Gomes, M.L., Freitas, R.S. and Bernardo-Filho, M., 2001. Model to evaluate the toxic effect of drugs: vincristine effect in the mass of organs and in the distribution of radiopharmaceuticals in mice. Mutation Research Journal, 496: 137-143.
- Mimica-Dukic, N., Bozin, B., Sokovic, M. and Simin, N., 2004. Antimicrobial and antioxidant activities of Melissa officinalis L. (Lamiaceae) essential oil. Journal of Agricultural and Food Chemistry, 52(9): 2485-2489.
- Mojab, F., Nickavar, B. and Hooshdar Tehrani, H., 2009. Essential oil analysis of Nepeta crispa and N. menthoides from Iran. Indian Journal of Pharmaceutical Sciences, 5(1): 43-46.
- Neuzil, J., Zhao, M., Ostermann, G., Sticha, M., Gellert, N., Weber, C.H., Eaton, J. and Brunk, U., 2002. Alpha-tocopheryl succinate, an agent with in vivo anti-tumour activity, induces apoptosis by causing lysosomal instability. Biochemical Journal, 362(3): 709-715.
- Philipson, J.D., 2001. Phytochemistry and medicinal plants. Phytochemistry journal, 56(3): 237-243.
- Ren, W., Qiao, Z., Wang, H., Zhu, L. and Zhang, L., 2003. Flavonoids: promising anticancer agents. Medicinal Research Reviews, 23(4): 519-534.
- Sefidkon, F., Jamzad, Z. and Mirza, M., 2006. Chemical composition of the essential oil of five Iranian Nepeta species (N. crispa, N. mahanensis, N. ispahanica, N. eremophila and N. rivularis). Flavour
and Fragrance Journal, 21(5): 764-767.
- Sonboli, A., Salehi, P. and Yousefzadi, M., 2004. Antimicrobial activity and chemical composition of the essential oil of Nepeta crispa Willd from Iran. Zeitschrift für Naturforschung C, 59:653-656.
- Verweil, J. and de Jonge, M.J.A., 2001. Achievements and future of chemotherapy. European Journal of Centre, 36(12): 1479-1487.
- Walker, K. and Croteau, R., 2001. Taxol biosynthesis: molecular cloning of a benzoyl-CoA: taxane 2alpha-O-benzoyltransferase cDNA from taxus and functional expression in Escherichia coli. Proceedings of the National Academy of Sciences, 97(25): 13591-13596.
- Wang, Q., Ruan, X., Huang, J.H., Xu, N.Y. and YAN, Q.C., 2006. Intra-specific genetic relationship analyses of Elaeagnus angustifolia based on RP-HPLC biochemical markers. Journal of Zhejiang University Science, 7(4):272-278.
- Wang, W., Li, N., Luo, M., Zu, Y. and Efferth, T.H., 2012. Antibacterial activity and anticancer activity of Rosmarinus officinalis L. essential oil compared to that of its main components. Molecules Journal, 17(3): 2704-2713.