Sh. Ahmadi; R,. Dehghani Bidgoli; R. Heydari Soreshjani
Abstract
Medicinal plants, in addition to their role in improving health and human quality of life, can have a positive impact on tourism capacity and tourist attraction. In this study, the economic potential of medicinal plants and its impact on tourism development in Abadanan city of Ilam province was investigated. ...
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Medicinal plants, in addition to their role in improving health and human quality of life, can have a positive impact on tourism capacity and tourist attraction. In this study, the economic potential of medicinal plants and its impact on tourism development in Abadanan city of Ilam province was investigated. The study method was descriptive-analytical with data gathering in the library and field through the distribution of a researcher-made questionnaire. The social classes studied in this study included businessmen, ordinary people, students and teachers. The collected data were analyzed using statistical tests and for a better comparison, the results were presented using structural modeling method by AMOS software. The results showed that the medicinal plants tourism affects the economic indicators item with a weighted regression of 0.52. Also, the economic potential of medicinal plants with the factor loading of 0.494 and family income through the sale of medicinal plants with the factor loading of 0.130 had the highest and lowest indirect impact on tourism of medicinal plants, respectively.
M. Nasseri; Sh. Golmohammadzadeh; H. Aroiee; M.R. Jaafari; H. Neamati
Abstract
In the present study, solid lipid nanoparticles (SLNs) were used as carriers of essential oil to overcome the problem of essential oil use (evaporation and degradation of some active components in the presence of air, light, moisture, and high temperatures) and increase the essential oil efficiency for ...
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In the present study, solid lipid nanoparticles (SLNs) were used as carriers of essential oil to overcome the problem of essential oil use (evaporation and degradation of some active components in the presence of air, light, moisture, and high temperatures) and increase the essential oil efficiency for controlling Alternaria solani, Rhizoctonia solani and Rhizopus stolonifer. This experiment was tested in vitro on PDA in Nanotechnology Research Center, School of Pharmacy, University of Medical Sciences of Mashhad and Faculty of Agriculture, Ferdowsi University of Mashhad. The study was conducted in a completely randomized design (CRD) whit three replications. Solid lipid nanoparticles containing essential oil at four concentrations of 20, 50, 100, and 200 ml per liter were applied on the potato dextrose agar medium. SLN containing Zataria multiflora Boiss. essential oil (ZM-SLN) were prepared by high shear homogenization and ultra sound method. The size of SLNs containing essential oil was less than 200 nm, and PdI and ZP were calculated to be 0.483 and -42.6 mv, respectively. The SLNs were spherical in shape. According to the obtained results, the minimum inhibitory concentration of the essential oil for all three fungi was 200 μlL-1. However, the minimum inhibitory concentration of SLN-ZM for Rh. stolonifer and R. solani was 50μlL-1, and for A. solani, it was calculated to be 100μlL-1. Our results clearly showed that SLNs could be suitable carriers for the Zataria multiflora essential oil.
Z. Movahedi; A. Moieni
Abstract
In Chicory (Cichorium intybus L.), leaf and root are the most important medicinal organs containing valuable secondary metabolites. In this research, the effect of foliar application of iron chelate and nano chelate fertilizers on morpho-physiological characteristics of chicory was studied in the aeroponic ...
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In Chicory (Cichorium intybus L.), leaf and root are the most important medicinal organs containing valuable secondary metabolites. In this research, the effect of foliar application of iron chelate and nano chelate fertilizers on morpho-physiological characteristics of chicory was studied in the aeroponic system.This system is a very suitable method for investigating the effect of nutrients and improving growth and development in different plants under controlled conditions. Experimental treatments included water (control), iron chelate (0.5, 1 and 1.5 g l-1) and iron nano chelate (0.5, 1 and 1.5 g l-1) fertilizers foliar application. Foliar spray was carried out at three stages including 20, 40 and 60 days after seed culture in the aeroponic system on leaf and root ina completely randomized design with five replications. After six months, some morphological and physiological traits were evaluated. The results of ANOVA indicated that the effect of different treatments was significant on plant height, root length, number and area of leaves, fresh and dry weight of root and shoot, photosynthetic pigments and iron content of root and shoot. The results of mean comparison showed that using 1.5 g l-1 of iron nano chelate resulted in the highest plant height (173.5 cm), root length (139.7 cm), root (65.2 g) and shoot (86.1 g) dry weight, leaf area (3448.5 mm2), chlorophylls a (2.43 mg g-1) and b (1.27 mg g-1) and carotenoid (1.25 mg g-1). The control treatment produced the lowest plant height (121.5 cm), root length (92.3 cm), root (15.1 g) and shoot (50.1 g) dry weight, leaf area (2259.6 mm2), chlorophylls a (1.46 mg g-1) and b (0.85 mg g-1) and carotenoid (0.76 mg g-1). In general, the results of this experiment showed a positive effect of iron nano chelate (with a concentration of 1.5 g l-1) foliar spray on the traits studied.
H. Khatami; M. Daneshyar; P. Farhoomand
Abstract
This experiment was conducted on breeder native hens (West Azerbaijan). For this purpose, two hundred and forty native hens (23 weeks old) were used in four experimental groups. The birds of each group were put in six replicate pens. Furthermore, a rooster was put in each cage for investigate the fertility ...
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This experiment was conducted on breeder native hens (West Azerbaijan). For this purpose, two hundred and forty native hens (23 weeks old) were used in four experimental groups. The birds of each group were put in six replicate pens. Furthermore, a rooster was put in each cage for investigate the fertility and hatchability of the hens. The birds of different groups were received the different levels of 0, 0.25, 0.50 and 1 percent turmeric rhizome powder. After the feeding the experimental diets for five weeks, 20 eggs were collected from each cages and used for incubation. There were no significant differences between the treatments for hatchability, fertility, embryo mortality and chick quality between treatments (p>0.05). Turmeric had no effect on blood total protein (p>0.05) but the highest turmeric consumption caused the increased blood cholesterol (p<0.05). Furthermore, blood lactate dehydrogenase and glutamate oxaloacetate transaminase activities were not affected by turmeric (p>0.05). However, all the turmeric levels decreased blood glutamate pyruvate transaminase activity as compared to control (p<0.05).
Improvement and breeding
M. Moghadami Rad; R.A. Khavari-Nejad; S. Saadatmand; F. Najafi
Abstract
Madder (Rubia tinctorum L.) is a herbaceous and perennial plant, which has bioactive compounds with high medicinal and pigment potency. Local farmers believe that increasing soil lime increases the color composition of this plant. To investigate the effects of calcium carbonate and phosphorus fertilizer ...
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Madder (Rubia tinctorum L.) is a herbaceous and perennial plant, which has bioactive compounds with high medicinal and pigment potency. Local farmers believe that increasing soil lime increases the color composition of this plant. To investigate the effects of calcium carbonate and phosphorus fertilizer on some physiological parameters and root alizarin content in madder, a field factorial experiment was conducted in a randomized complete block design with four replications. Experimental treatments included calcium carbonate at five levels (0, 20, 50, 100, and 150 kg.ha-1) and triple superphosphate at four levels (0, 50, 100, and 150 kg.ha-1). Means comparison showed that the highest root dry weight (an increase of 75% compared to control), total plant dry weight (an increase of 58% compared to control), root alizarin content (an increase of 78% compared to control), and root catalase activity were obtained with calcium carbonate increase up to 20 kg.ha-1 and triple superphosphate increase up to 100 kg.ha-1. The highest root length (an increase of 54% compared to control) and leaf catalase activity were observed in the 50 kg.ha-1 calcium carbonate and 100 kg.ha-1 triple superphosphate treatment. The highest leaf and root peroxidase activity was obtained in the treatment of 150 kg.ha-1 calcium carbonate and 150 kg.ha-1 triple superphosphate. Simultaneous application of these two fertilizers had a complementary effect on each other, so that the 20 and 50 kg.ha-1 calcium carbonate application along with the 100 kg.ha-1 triple superphosphate had an increasing effect on the alizarin content of madder root. This method can be used for the future development of a non-destructive tolerance test that shows madder increased response to calcium carbonate and phosphorus fertilizer, to further produce madder root medicinal compounds as a targeted drug for cancer treatment.
A. Lotfi; A.A. Vahabi Sedehi; A. Ganbari; M. Heydari
Volume 24, Issue 4 , February 2009, , Pages 506-518
Abstract
In order to investigate the effects of the two factors (deficit irrigation and manure) on yield and quality characteristic of the Plantago ovata Forssk. and experiment was conducted at the Research Farm, Zabol University. For this purpose split plot design based on completely randomized block with 4 ...
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In order to investigate the effects of the two factors (deficit irrigation and manure) on yield and quality characteristic of the Plantago ovata Forssk. and experiment was conducted at the Research Farm, Zabol University. For this purpose split plot design based on completely randomized block with 4 replications was used. Results of the variance analysis indicated that there were significant difference between irrigation levels on the yield and it’s five dependent traits except 1000 seeds weight and Biomass. The application of manure showed significant effect for all traits except 1000 seeds weight and Plant height. Levels of irrigation and manure had no significant effects on the quality characters such as amount of mucilage and swelling factor.
Agriculture and horticulture
F. Salarpour Qhoraba; H. Farahbakhsh
Abstract
To investigate the effects of chitosan foliar application on the various physiological and biochemical (proline, protein, antioxidant enzymes, and percentage and essential oil yield) characteristics and dry matter yield of thyme (Thymus vulgaris L.) under drought stress conditions, a split-plot experiment ...
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To investigate the effects of chitosan foliar application on the various physiological and biochemical (proline, protein, antioxidant enzymes, and percentage and essential oil yield) characteristics and dry matter yield of thyme (Thymus vulgaris L.) under drought stress conditions, a split-plot experiment was conducted in a randomized complete block design with three replications in Lalehzar city, Bardsir county, Kerman province in 2019. The drought stress at four levels (35, 50, 65, and 80% of available moisture discharge) and the chitosan concentration at five levels (0, 0.2, 0.5, 1.0, and 1.5 gram chitosan per liter of acetic acid) were considered as the main and sub plots, respectively. The results showed that the drought stress increased proline, antioxidant enzymes, and percentage and essential oil yield and decreased the dry matter yield significantly. The proline, antioxidant enzymes, dry matter yield, and essential oil percentage increased significantly with increasing the chitosan concentration, which resulted in a significant increase in the essential oil yield as the most important quality index in thyme. The protein and gayacol peroxidase enzyme were not affected by chitosan foliar application. In general, the results of this research showed that the higher chitosan concentrations (1.5 gram chitosan per liter of acetic acid) were more effective in ameliorating the damages induced by the drought stress and achieving the higher yield.
S. Saber Amoli; Sh. Noroozi; A. Shekarchian; M. Akbarzadeh; M. Kodoori
Volume 23, Issue 4 , February 2008, , Pages 532-543
Abstract
This research was conducted for identification of essential oil of Labiatae spesies and investigation of ecological characteristics of their habitates in Kerman province.The Labiatae is very important in production of essential oil. Ecological characteristics are required for cultivation of natural and ...
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This research was conducted for identification of essential oil of Labiatae spesies and investigation of ecological characteristics of their habitates in Kerman province.The Labiatae is very important in production of essential oil. Ecological characteristics are required for cultivation of natural and self growing plants of this family. Kerman province is about 186423 km3 in area and placed in southeast of Iran. Lowest and highest altitude in this province is 300m to 4500m a.s.l. and more than 10 kinds of climates are found in this area. 51 essential oil species belong to 17 genera. They are collected and identified by Flora. Many ecological characteristics of habitat are recorded as soil texture, rainfall, temperature, altitude, life form. More species belong to Nepeta and Salvia genera; chamaephyte and terophyte life formes; semiarid-cold, semiarid-warm and arid desert-cold; 2000-3000 altitude. Ziziphora tenuir L., Teucrium polium L., Nepeta glomerulosa Boiss. and Mentha longifolia (L.) Hudson are the most dispersed species in variety of climates, that indicates more ecological compitability of these species in different habitats.
M. Avijgan; M. Hafizi; M. Saadat
Abstract
The endemic Plants have different applications among people. Echinophora Platyloba is used in folk medicine, as a food seasoning.This study conducted to evaluate the effect of hydroalcoholic extract of Echinophora platyloba on Candida albicans.
Materials and Methods: By perculation method, hydroalcoholic ...
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The endemic Plants have different applications among people. Echinophora Platyloba is used in folk medicine, as a food seasoning.This study conducted to evaluate the effect of hydroalcoholic extract of Echinophora platyloba on Candida albicans.
Materials and Methods: By perculation method, hydroalcoholic extract was obtained. There 5 tubes of different diluted solution of extract were made as: 0, 35, 50, 150, 250 mg/ml for test. The yeast was purchased from faculty of medicine of Isfahan Unuiversity of Medical Sciences. By using agar dilution method, the yeast inoculated into all media for after 21 days of incubation period, when, the results were recorded.
The yeast was grown in control tube but no growth was observed in media containing difference diluitions of extract.
The results showed that 35, 50 and 150 mg/ml may have clinical application. This preliminary study showed that ethanolic extract of Echinophora platyloba has anti Candida albicans effect and may be in used in production of drug and clinical assay.
M.A. Soltanipoor
Volume 20, Issue 4 , January 2018, , Pages 547-560
Abstract
In this investigation, 10 important essential species of Hormozgan province from Labiatae family was studied.These species were Lavandula stricta,Mentha mozaffariani, Salvia aegyptiaca, S. mirzayanii, S. santolinifolia,S. sharifii,Teucrium pollium, T. stocksianum, Zataria ...
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In this investigation, 10 important essential species of Hormozgan province from Labiatae family was studied.These species were Lavandula stricta,Mentha mozaffariani, Salvia aegyptiaca, S. mirzayanii, S. santolinifolia,S. sharifii,Teucrium pollium, T. stocksianum, Zataria multiflora and Zhumeria majdae. With regard to source, search, inform persons and travelling to the various parts were determined ecological factors as: Habitate characteristics,local name, persian name, altitude, climate, rainfall, temperature, distributed regions, form, slope, campanion plants, soil texture, pH, EC, major compounds and traditional cure uses. Most of species were bushes. Used organ of them was leaf that used for digestive cures, romatism, fever,headache, cold, pain and wound. The major compounds of these plants were limonene, linalool and their derivatives that caused nice odour and tranquillizing properties. These plants located in mountainous regions, but some of them located in plains and hills. Altitude domain of habitates was varied between 10 to 2100 m. Soil texture of habitates was sandy-loam and loam with pH= 7.32 - 8.5 and EC= 0.130-1.357 mm/cm. Climates of speciesdistribution regions were hot and extradry, hot and semidry, hot and semidry deserty and hot and dry deserty. Average temperature was 17.5-27.5 average rainfall was 150-350 mm, although for more than species was 150-200mm. The habitate of species was sloppy rock with very low soil andtracks between rocks. Distribution of these species was very limited and thus formed.
H. Azarnivand; M. Ghavam Arabani; F. Sefidkon; A. Tavili
Abstract
Achillea millefolium L. subsp. millefolium is one of the valuable medicinal plants, belongs to Asteraceae family. This plant mainly grows in plaines, roadsides and mountainous regions. It is wound healer and because of having tannins, bitter and aromatic materials affects on the nervous system and heart. ...
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Achillea millefolium L. subsp. millefolium is one of the valuable medicinal plants, belongs to Asteraceae family. This plant mainly grows in plaines, roadsides and mountainous regions. It is wound healer and because of having tannins, bitter and aromatic materials affects on the nervous system and heart. Recent researches show that it has anti-cancer property. For sampling, this plant was selected from 2 height location with definite distance (100 meter) in Siahbisheh at full flowering stage in July 2007. The samples of inflorescences and leaves of plants and sample of soil of the root place were collected from 3 random points of each hieght at lenght of one transect (100 meter). Then air dried parts of the plants (80 gr) subjected to hydrodistillation for 2h using a Clevenger-type apparatus and essential oils analyzed by GC and GC/MC. There is no significant difference between the flower oil yields of Achillea millefolium subsp. millefolium in 2 height, while there is significant difference between the oil yields of leaves (p<0.01) and the highest percentage of leaf oil (0.1%) belong to 2100m. Study of relation among soil characters and height to these quantity show that there is relationship between nitrogen of the soil and this quantity that has negative correlation. Major components of flower oils were 1,8-cineole (14.4% and 8.2%), cis-cadin-4-en-7-ol (4.6% and 15%), E-caryophyllene (5.5% and 4.8%), -γmuurolene (4/4% and 5.2%), camphor (2.6% and 4.1%), eudesma-4(15), 7-dien-1-β-ol (3.7% and 6.4%) in 2100 m and 2200 m, respectively. The main components of leaf oils were 1,8-cineole (5.6% and 4.7%), globulol (7.6% and 8.7%), E-caryophyllene (5% and 3.2%), cis-cadin-4-en-7-ol (25.5% and 0.4%) E-sesqui lavandulol (2.3% and 12.8%) and E-sesquilavandulyl acetate (2.4% and 8.1%) at 2100 m and 2200 m, respectively.
S. Yousefzadeh; S.A.M. Modarres-Sanavy; F. Sefidkon; A. Asgarzadeh; A. ghalavand
Abstract
Dragonhead (Dracocephalum moldavica L.) is an annual plant that belongs to the (Labiate or Lamiaceae) family. The essential oil of dragonhead has natural antibacterial and antimicrobial substances and having wide usage in industries like pharmaceutical, dietary and many others. In this research, effects ...
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Dragonhead (Dracocephalum moldavica L.) is an annual plant that belongs to the (Labiate or Lamiaceae) family. The essential oil of dragonhead has natural antibacterial and antimicrobial substances and having wide usage in industries like pharmaceutical, dietary and many others. In this research, effects of different harvest times on content and composition of essential oil were studied in Research field at Khoy Agricultural Research Center in West Azarbaijan province. Dragonhead seeds from both landrace and modern cultivars were planted simultaneously. The aerial parts of plant were harvested in six stages. After drying the plant materials in shade, their essential oils were obtained by hydro-distillation. The oils were analyzed by GC and GC-MS. According to the results, maximum and minimum essential oils percentage for both landrace and modern cultivars were respectively observed in full flowering (0.53) and yellow-maturity stage (0.07). In addition, there were some differences between oil compositions of these dragonheadaccessions at different harvest times. The major oil components of dragonhead were the geranial, geraniol and geranyl acetate. The highest and the lowest sum total amount of geranial and geraniol were obtained at the early flowering (65.1%) and yellow-maturity (44.9%) for the landrace cultivar. The largest amount of geranyl acetate (50.7%) was gained for the modern cultivar at the early flowering stage while the least one (28.1%) was recorded for landrace cultivar plants 26 days after flowering when 10 to 30 percent of the flowers remained. The results showed that different harvest times had significant effects on essential oil content and composition.
A. Tadayyon; S. Torabian; M.R. Tadayon
Abstract
In order to evaluate the morphological and agronomical traits of linseed (Linum usitatissimum L.) genotypes in different plant densities, a field experiment was conducted as a factorial experiment in RCBD design with three replications in the field. Plant densities of 300, 500, and 1000 plant/m2 as ...
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In order to evaluate the morphological and agronomical traits of linseed (Linum usitatissimum L.) genotypes in different plant densities, a field experiment was conducted as a factorial experiment in RCBD design with three replications in the field. Plant densities of 300, 500, and 1000 plant/m2 as first factor and four linseed genotypes (Iran, Australia, Canada, and France) as second factor were used. Plants were harvested at three stages of vegetative growth, 50% flowering, and maturity stage. Results showed that plant densities had significant effect on plant height and above-ground dry weight in vegetative stage; plant height, stem diameter and dry weight in 50% flowering; and plant height, stem diameter, stem branch number, and dry weight in maturity stage. In addition, the number of capsules per plant, 1000 seeds weight, grain yield and harvest index were significantly affected by plant densities. Different linseeds genotypes showed significant effect on the characteristics of plant height, stem diameter, and stem branch number, in all three harvest stages. Traits as number of capsules per plant, number of seeds per capsule, 1000 seeds weight, grain yield, harvest index, and 50% of flowering and maturity also were affected significantly by linseeds genotypes. The maximum grain yield was obtained in the density of 1000 plants/m2. Maximum values were recorded for Iranian linseed genotype with an average production of 6.40 seeds per capsule, an average production of 1375 kg of seeds per hectare, and a mean harvest index of 21.23. It was also identified as the latest genotype for both days to 50% flowering (65 days) and days to maturity (123 days).
M. Rezae; R. Baradaran
Abstract
In order to improve seed yield and enhance the quality of medicinal plants, the establishment of sustainable agricultural aystems and utilization bio-fertilizers is considered of great importance. In order to study the effect of bio-fertilizers on yield and yield components of pot marigold (Calendula ...
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In order to improve seed yield and enhance the quality of medicinal plants, the establishment of sustainable agricultural aystems and utilization bio-fertilizers is considered of great importance. In order to study the effect of bio-fertilizers on yield and yield components of pot marigold (Calendula officinalis L.), an experiment was carried out as factorial in randomized complete blocks with 8 treatments and 3 replications in 2010. Treatments included compost (vermi-compost, city refuse compost, no organic fertilizer, granular organic fertilizer) and phosphate bio fertilizer (inoculated, not inoculated). Mean comparison was carried out using Duncan’s Multiple Range Test at 5% level). Results showed that phosphate bio fertilizer had not significant effects on plant height, flower yield, number of flower and grain harvest index but flower diameter, number of lateral stems, biomass yield, seed yield and flower harvest index wrer significantly affected. Also, significant differences were observed in most of the traits between treatments and control (no fertilizer, no inoculation). Biological fertilizers significantly increased plant height, total biomass yield, seed weight and harvest index however, flower diameter, number of secondary stems, dried flower yield, flower number per square meter and seed yield were not affected by treatments. The highest values of most of the traits were obtained at vermi-compost and phosphate bio fertilizer, affecting the yield components and seed yield of pot marigold positively.
M. Ghorbanli; Gh. Bakhshi Khaniki; A. Zakeri
Abstract
Water deficit is one of the most important factors affecting the growth of plants. In this research, Linum usitatissimum L. seeds were sown in plastic pots containing sand, clay and peat (2: 1: 1). When the third leave was appeared, water stress was done at three levels of control, 1/3 field capacity ...
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Water deficit is one of the most important factors affecting the growth of plants. In this research, Linum usitatissimum L. seeds were sown in plastic pots containing sand, clay and peat (2: 1: 1). When the third leave was appeared, water stress was done at three levels of control, 1/3 field capacity and 2/3 field capacity for 10 days in three duplication on the basis completely randomized design. The amount of carotenoid, anthocyanin, flavonoid, phenolic compound and proline was measured to study the effect of drought stress on non-enzymatic defense mechanisms. Data were subjected to analysis of variance by SPSS statistical software and means were compared by Duncan's test at p ≤ 0.05 significance level. It is noteworthy to state that proline accumulation in aerial parts of the Linum was more than that of the root. The results showed that the amount of praline, phenolic compound and anthocyanin significantly increased but the amount of flavonoid in 2/3 FC increased and then slightly decreased however it increased as compared with the control. In the current study, the amount of carotenoid in the leaves of Linum significantly decreased.
A. Khameseifi; F. Sefidkon
Abstract
Tanacetum polycephalum, belonging to the Asteraceae family, is a perennial and aromatic species with numerous medicinal properties. In this study, in order to evaluate and compare the quality and quantity of essential oil of flowering shoots and each of its parts (flowers, leaves and stems), the seeds ...
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Tanacetum polycephalum, belonging to the Asteraceae family, is a perennial and aromatic species with numerous medicinal properties. In this study, in order to evaluate and compare the quality and quantity of essential oil of flowering shoots and each of its parts (flowers, leaves and stems), the seeds of T. polycephalum Schultz. Bip. Subsp. polycephalum was collected from Kurdistan province (around the Qorveh city) and cultivated in the Alborz Agriculture Research Station, associated to the Research Institute of Forests and Rangelands. After collecting the aerial parts in full flowering stage, organs were separated and dried in the shade and essential oil extraction was carried out by hydro-distillation. The essential oils were analyzed by using a gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). The highest yield of essential oil (W/W relative to dry weight) was obtained from flowering shoots (0.87%) and the lowest yield was obtained from stems (0.12%). The oil yield of leaves and flowers was calculated to be 0.61% and 0.46%, respectively. The main compounds in the oil of flowering shoots were camphor (35.5%), cis- chrysanthenol (10.1%) and α-terpinene (9.5%). Camphor (25.4%), α-terpinene (13.2%), and cis-chrysathenol (11.2%) were found in the essential oil of leaves. Trans-β-farnesene (39.7%) and camphor (8.5%) were the main components in the essential oil of stems. According to the results, if camphor and cis-chrysanthenol is the target of essential oil extraction, distillation from flowering shoots and leaves is recommended; however, to achieve the most amount of trans-chrysanthenyl acetate, distillation from flowers is recommended.
Agriculture and horticulture
S. Azizi Balabiglou; A. Rahimi; S. Heydarzadeh; R. Holgoumi; I. Ataei
Abstract
To investigate the response of some quantitative and qualitative characteristics of peppermint (Mentha piperita L.) to organic fertilizers under low water stress, a factorial experiment was conducted in a randomized complete block design with three replications in the research farm of the Faculty of ...
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To investigate the response of some quantitative and qualitative characteristics of peppermint (Mentha piperita L.) to organic fertilizers under low water stress, a factorial experiment was conducted in a randomized complete block design with three replications in the research farm of the Faculty of Agriculture of Urmia University in 2017-2018 crop year. Experimental treatments included organic fertilizer at five levels (control, cattle manure (20 ton ha-1), poultry manure (7 ton ha-1), vermicompost (10 ton ha-1), and compost (15 ton ha-1)) and soil moisture at three levels (50 (severe stress), 70 (moderate stress), and 90% (control: without stress) of field capacity). Low water stress decreased the content of leaf phosphorus or potassium, leaf phenols or flavonoids, and leaf relative water content and increased the leaf essential oil yield at full flowering stage. The highest amount of essential oil (1.58%) was observed under moderate stress conditions. Poultry manure and vermicompost significantly increased the amount of nitrogen, protein, and percentage of DPPH radical scavenging of leaves under low water stress compared to without stress conditions. The highest yield of essential oil (32.34 kg ha-1), stem dry weight (852.53 kg ha-1), leaf dry weight (1010.40 kg ha-1), and aerial parts dry weight (1863.93 kg ha-1) was obtained in the poultry manure treatment under optimal irrigation. Overall, organic fertilizers application under low water stress could be recommended to increase the plant yield and achieve sustainable agriculture.
M. Ababaf; H. Omidi; A.M. Bakhshandeh
Abstract
This research was aimed to investigate the foliar application of Plant Growth Regulators (PGRs) on growth parameters, yield, some morphophysiological traits, and alkaloids of rosea periwinkle (Catharanthus roseus L. (G. Don) under drought stress conditions. The field study was conducted based on split-plot ...
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This research was aimed to investigate the foliar application of Plant Growth Regulators (PGRs) on growth parameters, yield, some morphophysiological traits, and alkaloids of rosea periwinkle (Catharanthus roseus L. (G. Don) under drought stress conditions. The field study was conducted based on split-plot factorial in the randomized complete block design (RCBD) with three replications at the research farms of Shahid Chamran University in Ahwaz city in 2016-17. The experimental treatments included drought stress as the main factor at three levels of 40% (control), 60% and 80% of maximum allowable depletion (MAD) and foliar application of jasmonic acid (JA) at two levels (0 and 10 µM) and salicylic acid (SA) at three levels (0, 0.1, 1, and 10 mM) as subplots. Based on the results of the mean comparison of main effects, drought stress (80% MAD) reduced 36.6% of fresh weight and 25.4% of the dry weight of the plant compared to the control. The treatment 10 mM SA resulted in 13.4, 125.5, and 59.6% increases in plant height, leaf area and flower dry weight, respectively, compared to the control. The foliar application of JA increased flower dry weight and plant fresh weight by 16.3 and 8.6%, respectively. The results showed that the foliar application of SA in concentrations of 1 and 10 mM caused the highest content of vincristine and vinblastine, respectively. The interaction of both PGRs and drought stress showed the highest alkaloids content. The highest vinblastine and vincristine under severe drought stress were obtained in foliar application of 1 mM SA+10µM JA and 10 mM SA+10µM JA, respectively.
A.R. Ghanbari; R. Moayed Amini; A. Estaji; S. Fahim; H. Ghorbani Ghozhdi
Abstract
Squirting cucumber (Ecballium elaterium (L.) Rich.) is a wild species that is native to temperate regions of Asia, North Africa, and Europe. This plant is one of the most important medicinal plants in the world. In the present study, morphological and biochemical traits of squirting cucumber ecotypes ...
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Squirting cucumber (Ecballium elaterium (L.) Rich.) is a wild species that is native to temperate regions of Asia, North Africa, and Europe. This plant is one of the most important medicinal plants in the world. In the present study, morphological and biochemical traits of squirting cucumber ecotypes were investigated from three natural habitats in Ardabil province including Germi, Bilesuar-Anjirlooin and Parsabad regions (10 genotypes per region). The results showed that Parsabad ecotypes had large fruits and higher chlorophyll and carotenoids content. Since the genetic diversity of this plant is not been thoroughly investigated, the genetic diversity of three squirting cucumber ecotypes were examined by using ISSR markers. To assess the genetic similarity between samples a cluster analysis was performed using Jacquard. Among primers, the primer ISSR-13 showed the most polymorphism (46%). The lowest genetic similarity was found between the two genotypes of Bilesuar and Germi population. Cluster analysis of molecular data was categorized into five groups. This preliminary study demonstrated that ISSR markers were an effective method to evaluate genetic variability among squirting cucumber plant genotypes.
Agriculture and horticulture
Afshin Tavakoli; Samaneh Asadi-Sanam; Arash Roozbahani
Abstract
Background and objectives: In producing medicinal plants, it is critical to use plants with a high percentage of active ingredients and consume low amounts of water. Thymus daenensis Celak is a tolerant Thymus species for rainfed cultivation. In addition, using complementary irrigation regimes and applying ...
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Background and objectives: In producing medicinal plants, it is critical to use plants with a high percentage of active ingredients and consume low amounts of water. Thymus daenensis Celak is a tolerant Thymus species for rainfed cultivation. In addition, using complementary irrigation regimes and applying anti-stress compounds at critical stages of plant growth are suitable programs for sustainable production and optimal water consumption under dry farming conditions. For this purpose, this experiment was to evaluate the effect of different complementary irrigation regimes along with sodium nitroprusside (SNP) as a nitric oxide donor, kaolin, and potassium spraying on yield and some biochemical characteristics of T. daenensis under rainfed conditions.Methodology: This experiment was designed and implemented as a split plot based on a randomized complete blocks design with three replications in the research field of the Research Institute of Forests and Rangelands-Homand Rangelands Research Station (Damavand) for three years (2017-2019 crop years). The experimental treatments included complementary irrigation as the main factor at three levels: control (no irrigation), one and two complementary irrigation, and spraying of stress-modulating substances as a secondary factor with three compounds of SNP (200 µM), kaolin (5%) and potassium (75 kg/ha from a potassium sulfate source). Cultivation was indirect and through seedlings. To prepare seedlings, the seeds were planted in the cultivation trays in the greenhouse at the end of September. After growing in the greenhouse, the seedlings (8-10 leaves) were transferred to the field in November with rain monitoring. Complementary irrigation and different foliar spraying treatments were done twice in the pre-flowering and 10 % flowering stages. Treatments were applied in the third year of plant growth to increase economic yield. After applying the treatments, the plant's flowering branch was sampled to measure malondialdehyde (MDA). After measuring the morphological characteristics (plant height and canopy diameter) in the full flowering stage, plant shoots were harvested to determine the dry matter yield per square meter and hectare. Percentage and yield of essential oil, total phenol, total flavonoids, and antioxidant capacity were also determined.Results: The results showed that increasing the number of complementary irrigations increased the crown diameter, plant height, and dry matter yield per plant and hectare. Kaolin treatment under two complementary irrigation regimes had the highest height (31.6 cm), and the control treatment under no irrigation had the lowest height (19.3 cm). Also, using kaolin and two complementary irrigations showed the highest dry matter yield per m2 (77.2 grams) and per hectare (771.4 kg). In addition, MDA content was lower in the double complementary irrigation treatment with kaolin foliar spraying (1.22 μmol/g of fresh weight). Foliar spraying of kaolin, potassium, and SNP under rainfed conditions (without irrigation) increased the amount of essential oil (by 1.83, 1.67, and 1.6 percent, respectively) compared to the control treatment (1.4 percent). In contrast, the maximum essential oil yield per hectare was related to the double complementary irrigation regime with potassium foliar spraying (10.6 kg/ha). Foliar spraying with potassium in the regimes of without irrigation (rainfed) and one-time complementary irrigation had the highest content of total phenols and flavonoids, respectively. The highest antioxidant capacity was obtained without irrigation and foliar spraying (control).Conclusion: This experiment showed that complementary irrigation increased dry matter yield per plant and hectare. On the other hand, foliar spraying with anti-stress compounds, especially kaolin, and potassium, along with complementary irrigation, increased essential oil yield per hectare. In general, based on the results of this experiment, it is suggested to use complementary irrigation regimes according to the available water sources. In addition, it is suggested to use kaolin or potassium foliar application under rainfed conditions for T. daenensis.
Gh. Eghlima; A. Kheiry; M. Sanikhani; Javad Hadian; M. Aelaei
Abstract
Licorice (Glycyrrhiza glabra L.) is one of the oldest and most important medicinal plants in Fabaceae, used for curing many diseases since 4000 years ago. This study was conducted to evaluate the genetic diversity of 22 different licorice populations based on morphological and yield traits at the research ...
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Licorice (Glycyrrhiza glabra L.) is one of the oldest and most important medicinal plants in Fabaceae, used for curing many diseases since 4000 years ago. This study was conducted to evaluate the genetic diversity of 22 different licorice populations based on morphological and yield traits at the research field of the Faculty of Agriculture, Zanjan University, during 2016 to 2018. Morphological and yield traits including plant height and width, leaf length and width, number, length and width of leaflets, number of lateral branches, main stem diameter, aerial parts fresh and dry weight, root fresh and dry weight, root to aerial parts ratio and aerial parts and root yields (per m2) were measured. Canonical discriminant (CDA) and cluster (CA) analyses were used to group the populations. In CDA, the first two canonical variables were significant. The first canonical variable included plant height and width, main stem diameter, leaf length and the number of leaflets, and the second one included aerial parts fresh and dry weight, root fresh and dry weight, root and aerial parts yields. The second canonical variable had the greatest role in population separation and grouping. Canonical variables divided populations into four main groups and confirmed CA clustering results. In general, the results indicated the good potential of canonical discriminant analysis in evaluating the genetic diversity and identifying the index traits in licorice.
A. Kazeminasab; M. Yarnia; M.H. Lebaschi; B. Mirshekar; F. Rajali
Abstract
Effects of vermicompost and plant growth promoting biofertilizers on the essential oil composition of lemon balm (Melissa officinalis L.) were studied under different irrigation conditions. The study was conducted in a split-split plot experiment based on a randomized complete block design with ...
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Effects of vermicompost and plant growth promoting biofertilizers on the essential oil composition of lemon balm (Melissa officinalis L.) were studied under different irrigation conditions. The study was conducted in a split-split plot experiment based on a randomized complete block design with three replications, at Research Institute of Forests and Rangelands.Treatments included irrigation in two levels (100 and 60% FC drought stress), vermicompost in three levels (0, 5 and 10t/ha) and plant growth promoting biofertilizers in four levels (Pseudomonas fluorescence, Azotobacter + Azospirillum, Azotobacter + Azospirillum + P. fluorescence, and the control). The essential oil components including citronellal, geraniol, neral, β-caryophyllene and methyl citronellat were studied. The interaction of irrigation × vermicompost showed significant effect on citronellal at P≤0.01 and on geraniol and methyl citronellat at P≤0.05. Mean comparison of irrigation indicated that the highest and lowest citronellal, geraniol, neral and β-caryophyllene, were obtained for drought stress and normal irrigation (100% FC), respectively. The highest and lowest methyl citronellat were recorded for the normal irrigation and drought stress (60% FC), respectively. Mean comparison of the effect of vermicompost showed that the highest (47.7%) and lowest (45%) citronellal were obtained for 10 t/ha vermicompost and control treatments, respectively. Application of 5 t/ha vermicompost resulted in the highest β-caryophyllene (6%). Mean comparison of the biofertilizers indicated that Azotobacter + Azospirillum + P. fluorescence had the highest citronellal (47.2%) and β-caryophyllene (5.9%); the lowest citronellal (45.6%) and β-caryophyllene (5.1%) were achieved in the control. Drought stress increased the essential oil compounds. Vermicompost just affected the citronellal. In addition, PGPR had a positive effect only on citronellal and β-caryophyllene.
J. Asghari; H.R. Sadeghipour; S.Kh. Hashemi Dost; M. Mazaheri Tehrani
Abstract
Oak acorn is an edible nut with a long history in human nutrition. In this study, the kernel fatty acid composition and tannin contents of chestnut leaved oak (Quercus castaneifolia C.A. Mey.) was investigated in Alangdarreh forest in Golestan province. Oil extraction from kernel was carried out by Soxhelt ...
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Oak acorn is an edible nut with a long history in human nutrition. In this study, the kernel fatty acid composition and tannin contents of chestnut leaved oak (Quercus castaneifolia C.A. Mey.) was investigated in Alangdarreh forest in Golestan province. Oil extraction from kernel was carried out by Soxhelt apparatus using n-hexane as solvent. Fatty acids were methyl esterified before quantitative and qualitative gas chromatography analysis. The results revealed the presence of saturated fatty acids including palmitic acid (C16:0), margaric acid (C17:0), stearic acid (C18:0) and unsaturated fatty acids such as oleic acid (C18:1), linoleic acid (C18:2), linolenic acid (C18:3) and gadoleic acid (C20:1). The net yield of extraction for oil was 7.49%, constituting 81.13% unsaturated and 18.87% saturated fatty acids. The three major identified fatty acids and their respective relative amounts were linoleic acid (49.57 mg.g-1 oil), oleic acid (34.74 mg.g-1 oil) and palmitic acid (20.58 mg.g-1 oil). Tannin extraction was carried out by water as solvent. The total tannin content of acorn was determined using gallic acid as standard spectrophotometrically. Results showed that the total tannin content of acorn was equivalent to about 178.13 mg galic acid.g-1 dry weight and the total oil content of acorn was 1.49 mg. 20 g-1 dry weights.
Biotechnology
Z. Abravesh; H. Zare; M. Khoshnevis
Abstract
Sorbus persica Hedl. is an Iranian endemic slow-growing tree (fam. rosaceae) and is endangered. It is important in terms of the gene storage, environmental protection, and medicinal uses. To in vitro propagate this species by the lateral bud culture, a factorial experiment was conducted in a completely ...
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Sorbus persica Hedl. is an Iranian endemic slow-growing tree (fam. rosaceae) and is endangered. It is important in terms of the gene storage, environmental protection, and medicinal uses. To in vitro propagate this species by the lateral bud culture, a factorial experiment was conducted in a completely randomized design in three levels of culture medium and three levels of cytokinin with three replications. For shooting, the DKW, WPM, and MS culture media containing the cytokinins BAP, Kin, and 2ip, and for rooting, the complete and modified DKW, WPM, and MS culture media containing the auxins NAA and IBA separately and in a consecutive application were investigated. The results showed that the best sterilization treatment was a consecutive application of sodium hypochlorite 20% (v/v, 15 min) and mercuric chloride 0.1% (3 min) in summer. The best proliferation and longitudinal growth of shoots was observed in the MS medium containing IBA (0.01 mg l-1), Kin (0.25 mg l-1), and BAP (3 mg l-1) growth regulators. Also, the best rooting was obtained in the DKW medium with a quarter of the concentration of macroelements containing the NAA+IBA hormones (0.3+0.3 mg l-1).
N. Ansari; N. Hasanzadeh; M.B. Rezaee
Abstract
One of the modern methods for biological control of button mushroom (Agaricus bisporus) brown blotch disease is using plant's essential oil. Therefore, antimicrobial properties of essential oil and extracts obtained from Eucalyptus camaldulensis Dehnh. leaves were evaluated against the plant pathogenic ...
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One of the modern methods for biological control of button mushroom (Agaricus bisporus) brown blotch disease is using plant's essential oil. Therefore, antimicrobial properties of essential oil and extracts obtained from Eucalyptus camaldulensis Dehnh. leaves were evaluated against the plant pathogenic bacterium Pseudomonas tolaasii both In vitro and In vivo conditions. 0.3 g/ml of each leaf samples was macerated in water and organic solvents (acetone, methanol and ethanol) to obtain the relevant extracts. The extraction of essential oil from leaves was performed Hydro-distillation method using Clevenger apparatus. Bioassays for inhibition activities of EO were carried out in five concentrations (0/1, 0/01, 0/001, 0/0001 and pure mg/ml) on two agar media of NA and KB. According to the isolation and identification of the main components in essential oils by gas chromatography (GC-MS), Cineol (58.1%) and α-phellandrene (6%) were identified as the main components. The most efficient In vitro results obtained by pure essential oil of Eucalyptus with 17 mm inhibition zone on KB and methanol extract with 8mm on NA. These were more pronounced when compared to inhibition effects of antibiotics erythromycin, penicillin and gentamycin and not with tetracycline in both concentrations of 0.1 and 0.01 mg/ml. This was reversed by subsequent increase of the antibiotics tetracycline and gentamicin to the level of 1, 5 and 10 mg/ml. In vivo assays were conducted in order to evaluate the efficacy of essential oil and methanol extract in two dilutions of 0/1 and 0/01 mg/ml. 20µl of each plant extracts was pre-treated on mushroom caps and after 24 h, the bacterial suspension at ca 105 cfu/ml was inoculated the same pre-treated sites. After a two day incubation period at 25°C, the 0/01 concentration of both extracts showed a satisfactory result.
