In collaboration with Scientific Association of Iranian Medicinal Plants

Document Type : Research Paper

Authors

1 Department of Chemistry, Science Faculty, Golestan University, Gorgan, Iran

2 Department of Plant Physiology, Science Faculty, Golestan University, Gorgan, Iran

Abstract

Oak acorn is an edible nut with a long history in human nutrition. In this study, the kernel fatty acid composition and tannin contents of chestnut leaved oak (Quercus castaneifolia C.A. Mey.) was investigated in Alangdarreh forest in Golestan province. Oil extraction from kernel was carried out by Soxhelt apparatus using n-hexane as solvent. Fatty acids were methyl esterified before quantitative and qualitative gas chromatography analysis. The results revealed the presence of saturated fatty acids including palmitic acid (C16:0), margaric acid (C17:0), stearic acid (C18:0) and unsaturated fatty acids such as oleic acid (C18:1), linoleic acid (C18:2), linolenic acid (C18:3) and gadoleic acid (C20:1). The net yield of extraction for oil was 7.49%, constituting 81.13% unsaturated and 18.87% saturated fatty acids. The three major identified fatty acids and their respective relative amounts were linoleic acid (49.57 mg.g-1 oil), oleic acid (34.74 mg.g-1 oil) and palmitic acid (20.58 mg.g-1 oil). Tannin extraction was carried out by water as solvent. The total tannin content of acorn was determined using gallic acid as standard spectrophotometrically. Results showed that the total tannin content of acorn was equivalent to about 178.13 mg galic acid.g-1 dry weight and the total oil content of acorn was 1.49 mg. 20 g-1 dry weights.

Keywords

- جهانشاهی، ش.، طبرسا، ت.، اصغری، ژ. و رسالتی، ح.، 1389. بررسی میزان اسید تانیک موجود در پوست بلوط بلندمازو (Quercus castanifolia). صنایع چوب و کاغذ ایران، 1(1): 35-27.
- صیامی، ع.، حیدری، ر.، پاکباز، ر. و آقازاده، م.، 1384. اندازه‌گیری تانن در چهار ژنوتیپ بلوط (Quercus infectoria Oliv.) و مصرف پودر گال آن در درمان زخم تجربی. تحقیقات گیاهان دارویی و معطر ایران، 21(4): 441-433.
- قادری قهفرخی، م.، صادقی ماهونک، ع.، اعلمی، م.، عزیزی، م. و قربانی، م.، 1391. تعیین فعالیت آنتی اکسیدانی عصاره‌های فنولی میوه یک واریته بلوط ایرانی (Q. castaneifolia var. castaneifolia). علوم و صنایع غذایی، 9(35): 56-45.
- Alemzadeh, I., Vossoughi, M. and Maghsoodi, V., 2000. An investigation of chemical and physical properties of Kordestan (Iran) acorn. Journal of Agricultural Science and Technology, 2(3): 225-228.
- Charef, M., Yousfi, M., Saidi, M. and Stocker, P., 2008. Determination of the fatty acid composition of acorn (Quercus), Pistacia lentiscus seeds growing in Algeria. Journal of Oil and Fat Industries, 85(10): 921-924.
- Hageraman, A.E. and Butler, L.G., 1991. Tannins and lignins: 355-388. In: Rosenthal, G.A. and Berenbaum, M.R., (Eds.). Herbivores: Their Interactions with Secondary Plant Metabolites (Vol. 1: The chemical participants). Academic Press, 468p.
- Haslam, E., 1989. Plant Polyphenols. Cambridg University Press, 230p.
- Johnson, P.S., Shiflev, S.R. and Rogers, R., 2002. The Ecology and Silviculture of Oaks. CABI Publishing, Wallingford, Oxon, UK, 544p.
- Khanbabaee, K. and van Ree, T., 2001. Tannins: classification and definition. Natural Product Reports, 18(6): 641-649.
- Krishnaiah, D., Devi, T., Bono, A. and Sarbatly, R., 2009. Studies on Phytochemical constituents of six Malaysian medicinal plants. Journal of Medicinal Plants Research, 3(2): 67-72.
- Metcalf, L.D. and Shmitz, A.A., 1966. Rapid preparation of methyl esters from lipid for gas chromatography analysis. Analytical Chemistry, 38(3): 514-515.
- Okuda, T., 2005. Systematics and health effects of chemically distinct in medicinal plants. Phytochemistry, 66(17): 2012-2031.
- Panahi, P., Jamzad, Z., Pourmajidian, M.R., Fallah, A. and Pourhashemi, M., 2011a. A revision of chestnut-leaved oak (Quercus castaneifolia C. A. Mev. Fagaceae) in Hyrcanian forests of Iran. Caspian Journal of Environmental Sciences, 9(2): 145-158.
- Panahi, P., Jamzad, Z., Pourmajidian, M. R., Fallah, A. and Pourhashemi, M., 2011b. Importance of micromorphological characteristics of foliar and pollen grains for delimitation of oak species in Iran. Iranian Journal of Forest and Poplar research. 19(1): 163-179.
- Rakic, S., Petrovic, S., Kukic, J., Jadranin, M., Tesevic, V., Povrenovic, D. and Siler-Marinkovic, S., 2007. Influence of thermal treatment on phenolic compounds and antioxidant properties of oak acorns from Serbia. Food Chemistry, 104(2): 830-834.
- Saffarzadeh, A., Vincze, L. and Csap, J., 1999. Determination of the chemical composition of acorn (Quercus branti), Pistacia atlantica and Pistacia Khinjuk seed as non-conventional feedstuff. Acta Agraria Kaposvariensis, 3(3): 59-69.
- Sagheb Talebi, Kh., Saiedi, T. and Pourhashemi, M., 2014. Forests of Iran: a treasure from the past a hope for future. Springer Publication, 152p.
- Shimada, T., 2001. Nutrient compositions of acorns and horse chestnuts in relation to seed-hoarding. Ecological Research, 16(4): 803-808.
- Tejerina, D., Garcia-torres, S., Cabeza de Vaca, M., Vazquez, F.M. and Cava, R., 2011. Acorns (Quercus rotundifolia Lam.) and grass as natural sources of antioxidants and Fatty acids in the "montanera" feeding of Iberian pig: Intra-and inter-annual variations. Food Chemistry, 124(3): 997-1004.