F. Najafi; Z. Tavakkoli
Abstract
In the present study, essential oil composition and antibacterial effects of Ziziphora tenuior in Hesarak (Karaj) and Venarch (Qom) were studied and compared. The aerial parts of plants were collected at full flowering stage and essential oil was isolated by hydrodistillation and analyzed by capillary ...
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In the present study, essential oil composition and antibacterial effects of Ziziphora tenuior in Hesarak (Karaj) and Venarch (Qom) were studied and compared. The aerial parts of plants were collected at full flowering stage and essential oil was isolated by hydrodistillation and analyzed by capillary GC and GC-MS. Thirteen and sixteen compounds were identified in essential oils of plants in Hesarak and Venarch respectively. In both regions, the highest content was recorded for pulegone (85 to 87 %) and no significant differences were observed. Limonene in plants of Hesarak (5.1%) were more than that of Venarch(3.64 %). Ethanol extracts of the plants in Hesarak significantly showed an effective controlling and antimicrobial effect against Escherichia coli, Bacillus subtilis, and Staphylococcus aureus compared with plants of Venarch. Concentrations of Mn, Fe and Ni in soil of Venarch (2766.67, 40333, 0.384 ppm respectively) were higher than that of Hesarak (558.33, 28600, 12.25 ppm respectively), however, soluble manganese and iron contents in soil of Hesarak (6.53 and 4 ppm respectively) were higher than that of soil of Venarch (4.6 and 1.9 ppm respectively). Since concentration of Mn, Fe and Ni in aerial parts and roots of the studied plant in Hesarak and Venarch showed significant difference, antibacterial activity of the plants in both regions is also different. According to the results and with regard to the changes in elements concentration of the soil, essential oil composition and antibacterial effects of Ziziphora tenuior are affected by elements content in soil.
M.M. Attarpour Yazdi; M. Kamalinejad; N.S. Falvaei Koochak; S. Mansouri
Abstract
Burn wound is suitable site for incidence of resistant infections. Thus, the research for finding effective drugs against this problem is necessary. Medicinal herbs with antimicrobial activity have important role in traditional medicine. The purpose of this study was to determine antibacterial activity ...
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Burn wound is suitable site for incidence of resistant infections. Thus, the research for finding effective drugs against this problem is necessary. Medicinal herbs with antimicrobial activity have important role in traditional medicine. The purpose of this study was to determine antibacterial activity of methanolic extract of Terminalia catappa L. fruitagainst bacteria isolated from burn wound infections and to compare with effects of some selected antibiotics. First, a sample of methanolic extract of the Terminalia catappa fruit was prepared and then its antibacterial activity against 8 bacteria from 100 samples of burn wound infection were evaluated by well diffusion method at concentration of 40 mg/ml and then Agar Serial Dilution method in the range of 0.039-20mg/ml. Also, the MIC (Minimal Inhibitory Concentration) of extract was determined. The antibacterial activity of selective antibiotics was tested by disk diffusion method. The onava test was used to compare the results. The results from the antibacterial tests demonstrated that the Terminalia catappa methanolic extract had been effected against all of Pseudomonas aeruginosa, Acinetobacter spp. and more than 80% of Staphylococcus aureus/epidermidis/saprophyticus, and against 50% of Escherichia coli. The MIC of the extract against all the sensitive cases was 20 mg/ml. The bacteria were often resistant to selective drugs .There was significant difference between the effects of plant and antibiotics on Pseudomonas aeruginosa (P<0/001), Acinetobacter spp. (P<0/05) and Staphylococcus cases (P<0/05). This study demonstrated that methanolic extract of Terminalia catappa have excellent antibacterial activity against most of bacteria isolated from burn wound infections and its effect is better than selective antibiotics. However, we need more in vitro and in vivo investigation.
M.R. Jalali; H. Jafari; P. Owlia; N. Fallah; A. Davati
Abstract
Salmonellosis is one of the most common infections in developing countries. Antibiotics used for management, may led not only to drug-resistance, but also to some serious complications. Since antiquity medicinal plants have been used for treatment of diseases, Garlic is one of these agents with multiple ...
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Salmonellosis is one of the most common infections in developing countries. Antibiotics used for management, may led not only to drug-resistance, but also to some serious complications. Since antiquity medicinal plants have been used for treatment of diseases, Garlic is one of these agents with multiple effects i.e. antibacterial effects. Sixty rabbits of Dutch-polish race were randomly divided in three equal groups; contaminated with no treatment as the control group, contaminated with treatment by low and high-doses of garlic aqueous extract in the other groups. All rabbits were infected with 1011 CFU of S. typhimurium fourty-eight hours after contamination, garlic aqueous extract was used in treated-groups at 20mg/kg and 40mg/kg three times a day. Stool culture is performed at first before contamination and after 24, 48, 72, 96 and 168 hours of treatment. Bacterial colony count stool showed significant statistics in different days. A significant difference was also seen in colony count of treated groups with non-treated group (p<0.001). But there was no definite difference in stool colony count of treated groups (low dose & high dose). So garlic aqueous extract has antibacterial effects on S.typhimurium. Further studies are recommended for clinical usage of this agent.
