Biological effects of essential oils and extracts
Hanieh Mohseni fazel; Karim Nozad namini; Hossein Hoseini; H. Zeinali
Abstract
Background and Objectives: Pigweed (Amaranthus retroflexus L.) is one of the most widespread weeds in the world, and regarding the amount of damage caused to crops, it is the third dominant dicotyledonous weed in the world. It is recommended to reduce the use of chemical herbicides ...
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Background and Objectives: Pigweed (Amaranthus retroflexus L.) is one of the most widespread weeds in the world, and regarding the amount of damage caused to crops, it is the third dominant dicotyledonous weed in the world. It is recommended to reduce the use of chemical herbicides through the replacement of non-chemical methods in sustainable agricultural management, and the use of plants' allelopathic effects is one of these methods. On the other hand, plants in different growth stages have different allelopathy sensitivity.Methodology: To evaluate the allelopathic effect of aqueous and alcoholic extracts of eucalyptus (Eucalyptus camaldulensis Dehnh) on Pigweed (Amaranthus retroflexus) growth stages, an experiment was carried out as a factorial based on a randomized complete block design with three replications in the greenhouse of Kashan Barij Essential Company, Iran. Treatments included aqueous and alcoholic extracts of eucalyptus (50, 75, and 100 %), controlled (0.002 concentration of 2,4-Dichlorophenoxyacetic acid, Ethanol 70%, distilled water), and three growth stages (five-leaf stage, seven-leaf stage, and stem elongation). The studied traits were measured in all three growth stages one week after treatments. To measure traits, ten plants were randomly selected from each pot. The studied traits included leaf length and width, total leaf wet weight, total leaf dry weight, stem wet weight, stem dry weight, stem length, root length, root wet weight, root dry weight, total plant weight, and shoot/root ratio. A ruler was used to determine the length and width of the leaf, the length of the root, and the length of the stem. Analysis of the variance of the data was done by MSTAC software. If the experimental factor was significant, the LSD test was used to compare the means.Results: Leaf width, total leaf fresh weight, total leaf dry weight, stem fresh and dry weight, stem length, root length, root fresh and dry weight, total plant weight, and shoot/root ratio were affected by the growth stage where the extracts were used. The effect of the concentration of aqueous and ethanol extracts of eucalyptus on leaf length and width, total leaf fresh weight, total leaf dry weight, stem fresh weight, stem dry weight, stem length, root length, root fresh weight, root dry weight, total dry plant weight, and shoot/root ratio was significant. Spraying at the stem and 5-leaf stages had the highest and lowest leaf width, stem length, and root dry weight, respectively. The extract application at the stem elongation and five-leaf stage had the highest and lowest leaf width, stem length, and root dry weight, respectively. The application of distilled water in the stem elongation phase produced the maximum leaf length (3.06 cm), total fresh and dry weight of plant leaf (1.00 and 0.2764 gr), stem fresh and dry weight (1.35 and 0.393 gr), and total fresh and dry plant weight (2.80 and 0.801 gr), respectively. The application of 75% concentration of eucalyptus ethanol extract at the 5-leaf stage produced the lowest total fresh and dry weight of plant leaf (0.188 and 0.0521 gr), stem fresh and dry weight (0.303 and 0.046 gr) and total fresh and dry plant weight (0.633 and 0.1157 gr), respectively.Conclusion: The aqueous and alcoholic extracts significantly decreased Pigweed growth indices, and its inhibitory effect was not significantly different in most traits with 2-4-D herbicide. The inhibitory effect of alcoholic extract on pigweed growth was greater than aqueous extract. Given that almost all eucalyptus extracts, either alcoholic or aqueous, significantly reduced Pigweed growth, it may be possible to reduce the pressure of this weed on crops by combining and using them as a bio-herbicide.
M. Tohidi; A. Vaziri; A.A. Hatamnia
Abstract
Some plants have negative effects on growth of the adjacent plants through excretion chemical Some plants have negative effects on the growth of adjacent plants through excretion chemical compounds known as allelopathy. In this study, the allelopathy effects of different parts of Heliotropium europaeum ...
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Some plants have negative effects on growth of the adjacent plants through excretion chemical Some plants have negative effects on the growth of adjacent plants through excretion chemical compounds known as allelopathy. In this study, the allelopathy effects of different parts of Heliotropium europaeum L. on seed germination and seedling growth of Raphanus sativus L. were investigated. The treatments included the extracts of root, stem, leaf, flower, fruit and distilled water (control) on seed germination of R. sativus. Also, 1% of stem powder, the whole plant as mulch and the whole plant mixed with soil were studied. To assess the allelopathic effects of different parts of H. europaeum, a number of growth parameters such as seed germination, stem length as well as dry and fresh weight were evaluated. The results indicated that all parts of the plant had a significant effect on germination of radish seeds, but their effects were different, so that the flower and stem extracts had the maximum inhibitory effect. The comparison of stem length, fresh and dry weight of R. sativus seedling in control and different treatments of H. europaeum showed that there was a significant difference between different treatments and control samples. Among different treatments, the inhibitor effects of stem extract was higher than other extracts. It was concluded that H. europaeum extract especially flower and stem extracts had a strong allelopathic effect and inhibited seed germination of R. sativus and could berecommended in production of natural herbicides.
M. Alirezaie Noghondar; M. Azizi; P. Taheri; M.S. Sadeghi
Abstract
To investigate the allelopathic potential and its relation with phenolic changesin different concentrations of methanolic and ethanolic extracts from root and leaf of Rumex turcomanicus Czerep.on seed germination and seedling growth of lettuce (Lactuca sativa L.), a factorial experiment based on completely ...
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To investigate the allelopathic potential and its relation with phenolic changesin different concentrations of methanolic and ethanolic extracts from root and leaf of Rumex turcomanicus Czerep.on seed germination and seedling growth of lettuce (Lactuca sativa L.), a factorial experiment based on completely randomized design was conducted at the Faculty of Agriculture, Ferdowsi University of Mashhad, Iran, in 2013. Treatments included different organs of Rumex turcomanicus Czerep (root and leaf), two extraction solvents (methanol and ethanol), and six concentrations of extract (0, 31.25, 62.5, 125, 250 and 500 ppm), whose effects were studied on seed germination and seedling growth of lettuce. The extracts were placed in petri dishes in three replicates and 25 seeds (lettuce) per replicate. Phenolic content was measured in each extract. The results showed higher inhibition in methanoilc extracts as compared with ethanolic extracts and higher inhibition in root extract as compared with leaf extracts on seed germination and most of seedling the growth traits. Among different concentrations, maximum and minimum inhibition were observed in 500 and 0 ppm of extracts, respectively. Phenolic content was greater in methanolic as compared with ethanoilc extracts and was higher in root extracts as compared with leaf extracts. In addition, phenolic content was increased with increasing of extract concentration. A positive and significant correlation was observed between phenolic content and allelopathic potential.
H. Delavar; M.J. Saharkhiz; N. Kazerani
Abstract
This investigation was conducted to determine the essential oil composition and allopathic properties of anghuzeh (Ferula assa-foetida L.) medicinal plant. The experiment was completely randomized design in a factorial arrangement with two factors and three replications. The factors were six concentrations ...
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This investigation was conducted to determine the essential oil composition and allopathic properties of anghuzeh (Ferula assa-foetida L.) medicinal plant. The experiment was completely randomized design in a factorial arrangement with two factors and three replications. The factors were six concentrations of 0, 200, 400, 600, 800 and 1000 ppm of the essential oil and five plant species including three weed species, Sinapis arvensis, Acroptilo repens, and Cardaria draba as well as two crops i.e. Triticum aestivum and Hordeum vulgare. The essential oil used in this study was extracted by hydro-distillation from the latex of the plant. The obtained oil was analyzed by Gas chromatography (GC) and Gas chromatography–mass spectrometry (GC-MS).To assess the allopathic effects of the oil on the studied species, a number of growth parameters such as seed germination, radical and plumule length as well as their dry and fresh weight, and abnormality percent of the seedlings were evaluated. The results of this investigation showed that the Anghuzeh essential oil had significant (p ≤ 0.05) allelopathic effect on the studied weeds and crops. The comparisons among the means indicated that the response of the species to the oil were quite different. For example, Triticum aestivum showed maximum germination percent in the presence of different oil concentrations. While, the germination of Hordeum vulgare, Acroptilon repens, and Cardaria draba showed more susceptibility to the oil. Moreover, the results of the essential oil analysis indicated that a total of 13 components were identified in the oil of which α-Pinene (21.3%), β- Pinene, (47.1%) and 1,2-Dithiolane (18.6%) were the major compounds. The observed allelopathic effects of the essential oil are regarded to oil components and their synergistic effects. The findings of this study are the first step to use of Anghuzeh essential oil as a natural herbicide. Further investigations are still required for practical and commercial application of this method.
M. Sakhayi; M.H. Assareh; A. Shariat; Gh. Bakhsi Khaniki; M. Matinizadeh
Abstract
In this study, allelophatic effects of Eucalyptus camaldulonsis Dehnh. were investigated on some growth parameters and quantitative and qualitative changes of peroxidase in wheat at greenhouse of research institute of forests and rangelands of Iran. Treatments included: control treatment, etanol extract ...
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In this study, allelophatic effects of Eucalyptus camaldulonsis Dehnh. were investigated on some growth parameters and quantitative and qualitative changes of peroxidase in wheat at greenhouse of research institute of forests and rangelands of Iran. Treatments included: control treatment, etanol extract of E. camaldulonsis leaf in three levels (3, 6, 12 g/lit), aqueous extract of E. camaldulonsis leaf in three levels (5%, 10%, 20%), fresh leaf of E. camaldulonsis in three levels (5, 10, 15 g), powderd dried leaves of E. camaldulonsis in three levels (5, 10, 15 g) and one-year seedlings of E. camaldulonsis. Wet and dry weight of the seedlings was measured as growth parameters. Comparison of different treatments showed that one-year seedlings of E. camaldulonsis had maximum significant effect in reducing wheat growth parameters due to root exudates. With increasing concentrations of ethanol and aqueous extracts, wet and dry weight and leaf surface area of wheat decreased while in treatments of fresh and powdered dried leaves, an increment was observed in dry weight and leaf area. Activity of peroxidase enzyme strongly increased in root treatment of E.camaldulonsis and showed new isoenzyme band compared to control treatment. Ethanol and aqueous extracts of Eucalyptus leaves increased peroxidase activity in wheat but no new isoenzyme band was observed and less affected in fresh and dry leaves of Eucalyptus.
M. Makkizadeh Tafti; R. Farhoudi; M. Rabiee; M. Rasifar
Abstract
Nowadays, in response to the consequences of herbicide application and gradual reduction of farm crops, suitable biological methods have been identified to control the weeds. This research was conducted to evaluate the allelopathic effect of harmel (Peganum Harmala L.) on seed germination of oat (Avena ...
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Nowadays, in response to the consequences of herbicide application and gradual reduction of farm crops, suitable biological methods have been identified to control the weeds. This research was conducted to evaluate the allelopathic effect of harmel (Peganum Harmala L.) on seed germination of oat (Avena fatua L.), fat hen (Chenopodium album L.) and pig weed (Amaranthus retroflexus L.) in laboratory and greenhouse conditions using hydroalcoholic extracts of harmel dried fruit powder. The treatments were 0.1, 0.25, 0.5, 1, 2.5 and 5% extract of harmel and distilled water (control). Laboratory results indicated that germination percentage and radicle and plumule lengths of weed species were significantly reduced by different harmel extracts in comparison with control. According to the results of greenhouse experiments, germination percentage, fresh and dry weight and height of weed species were significantly reduced by using hydroalcoholic extracts. Greenhouse results also showed that seed germination percentage of Chenopodium album, Amaranthus retroflexus and Avena fatua at concentration of 1% was reduced 60%, 50% and 40% respectively in comparison with control. Our results showed that harmel extract had strong allelopathic effects and inhibited seed germination of weeds recommended in production of natural herbicides.
M. Makkizadeh Tafti; M. Salimi; R. Farhoudi
Volume 24, Issue 4 , February 2009, , Pages 463-471
Abstract
The allelopathic effects of rue (Ruta graveolens L.) were evaluated on seed germination of velvet flower (Amaranthus retroflexus), flixweed (Descurainia Sophia) and purslane (Portulaca oleracea) in laboratory using the aqueous extracts of dried powdered of rue leaves. The treatments were 0.5, 1, 2.5, ...
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The allelopathic effects of rue (Ruta graveolens L.) were evaluated on seed germination of velvet flower (Amaranthus retroflexus), flixweed (Descurainia Sophia) and purslane (Portulaca oleracea) in laboratory using the aqueous extracts of dried powdered of rue leaves. The treatments were 0.5, 1, 2.5, 5, 10 and 15% extract of Ruta graveolens and distilled water (control). According to the results, extracts significantly inhibited seed germination of weed species and the degree of inhibition increased with increasing concentration of extracts. Germination of Amaranthus retroflexus seeds was inhibited at concentrations greater than 5%. In addition, radicle and plumule lengths of Amaranthus retroflexus were significantly reduced at 1% compared to the distilled water. Results indicated germination percentage, germination rate and radicle and plumule lengths of Portulaca oleracea were significantly reduced by the extracts compared to the distilled water. Results confirmgermination of Descurainia sophia seeds was inhibited at concentrations greater than 2.5%. Accordingly germination rate and radicle lengths of Descurainia sophia were significantly reduced by the extracts compared to the distilled water. Therefore, extract of rue might be useful as natural herbicides and might also contain numerous growth inhibitors that could be used for the development of biological herbicides.
M.A. Soltanipor; A. Hajebi; A. Dastjerdi; S. Ebrahimi
Volume 23, Issue 1 , May 2007, , Pages 51-58
Abstract
In this investigation, effects of aqueous extractfrom leaves of Zhumeria majdae on seed germination of Lycopersicon esculentum, Lactuca sativa, Brassica oleracea, Allium porrum, Raphanus sativus, Allium cepa, Lipidium sativum were studied. Aqueous extract from leaves of Zhumeria majdae inhibited seed ...
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In this investigation, effects of aqueous extractfrom leaves of Zhumeria majdae on seed germination of Lycopersicon esculentum, Lactuca sativa, Brassica oleracea, Allium porrum, Raphanus sativus, Allium cepa, Lipidium sativum were studied. Aqueous extract from leaves of Zhumeria majdae inhibited seed germination. In the Lipidium sativum and Brassica oleracea, seed germination rate was reduced to zero. Statistically, In percentage of seed germination, there were more significant differences between aqueous extract and control in Allium cepa, Lipidium sativum and Brassica oleracea. There were significant differences in Lactuca sativa and Allium porrum and no significant differences were found in Lycopersicon esculentum and Raphanus sativus. In speed of seed germination, there were more significant differences between aqueous extract and control in Lycopersicon esculentum, Lactuca sativa, Brassica oleracea, Allium porrum, Allium cepa and Lipidium sativum and significant differences in Raphanus sativus.
M. Azizi; L. Alimoradee; M.H. Rashedmohassel
Volume 22, Issue 3 , November 2006, , Pages 198-208
Abstract
In order to investigate the allelopathic effects of the essential oils of Bunium persicum and Cuminum cyminum, this research conducted in two separate experiments to find out the effects of different essential oils concentration of Bunium persicum (200, 500, 700, 1000 and 2000 ppm) and Cuminum cyminum ...
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In order to investigate the allelopathic effects of the essential oils of Bunium persicum and Cuminum cyminum, this research conducted in two separate experiments to find out the effects of different essential oils concentration of Bunium persicum (200, 500, 700, 1000 and 2000 ppm) and Cuminum cyminum (100, 300, 500, 700 and 1000 ppm) on germination activity of Bromus tectorum, Centura ovina and Descurainia sophia. Each experiment was conducted in RCBD with 4 replications. Results showed that germination percentage of all the weeds were high in control plots( 96.67, 73 and 96.67 respectively). Germination of Centura ovina and Bromus tectorum were completely inhibited by Bunium persicum essential oils at 700 and 1000 ppm respectively. Descurainiaseeds were more sensitive to Bunium persicum essential oils and its germination completely inhibited at 500 ppm . There was no significant difference between 200 ppm and 500ppm of Bunium persicum essential oils as germination percentage of Bromus tectorum as concerned (54% and 44% respectively). Bunium persicum essential oils at 500ppm decreased germination percent of Centura ovina by 25% ( from 73% to 48.67% ). But 200ppm Bunium persicum essential oils decreased germination percent of Descurainia sophia by 69% (from 96.67 to 27.67). Cuminum cyminum essential oils also affected germination process of all the weeds that were tested in the experiments. Germination of Bromus tectorum, Centura ovina and Descurainia sophia completely inhibited by 2000, 1000 ppm and 500 ppm, respectively. Concentration increasing of Cuminum cyminum essential oils from 100ppm to 1000ppm decreased germination percent of Bromus tectorum, Centura ovina and Descurainia sophia by 46.67%, 51% and 53 % respectively. In respect to germination rate data showed that all the treatments decreased germination rate and it decreased from 11.25 , 4.14 ,17.5 seed/day in control plot to zero in treated seeds with Bunium persicum essential oils. Germination rate of Bromus,Centura and Descurainia were also affected by Cuminum cyminum treatments and it decreased from 18.77, 5.82 and 13.6 in control plot to zero respectively. It was concluded that Bunium persicum and Cuminum cyminum essential oils as an allelopathic agents for the weeds control should be a good agent for organic culture.
