Document Type : Research Paper
Authors
Abstract
In this study, allelophatic effects of Eucalyptus camaldulonsis Dehnh. were investigated on some growth parameters and quantitative and qualitative changes of peroxidase in wheat at greenhouse of research institute of forests and rangelands of Iran. Treatments included: control treatment, etanol extract of E. camaldulonsis leaf in three levels (3, 6, 12 g/lit), aqueous extract of E. camaldulonsis leaf in three levels (5%, 10%, 20%), fresh leaf of E. camaldulonsis in three levels (5, 10, 15 g), powderd dried leaves of E. camaldulonsis in three levels (5, 10, 15 g) and one-year seedlings of E. camaldulonsis. Wet and dry weight of the seedlings was measured as growth parameters. Comparison of different treatments showed that one-year seedlings of E. camaldulonsis had maximum significant effect in reducing wheat growth parameters due to root exudates. With increasing concentrations of ethanol and aqueous extracts, wet and dry weight and leaf surface area of wheat decreased while in treatments of fresh and powdered dried leaves, an increment was observed in dry weight and leaf area. Activity of peroxidase enzyme strongly increased in root treatment of E.camaldulonsis and showed new isoenzyme band compared to control treatment. Ethanol and aqueous extracts of Eucalyptus leaves increased peroxidase activity in wheat but no new isoenzyme band was observed and less affected in fresh and dry leaves of Eucalyptus.
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