Biotechnology
F. Noormand Moaied; N. Valizadeh; T. Samandari Giklo
Abstract
Silybum marianum L., from fam. asteraceae, is a very important medicinal plant in the pharmaceutical industry due to its active ingredient silymarin. Silymarin is very effective in treating the liver diseases, hepatitis, and cardiovascular disorders. Since the potential and production speed of this medicinal ...
Read More
Silybum marianum L., from fam. asteraceae, is a very important medicinal plant in the pharmaceutical industry due to its active ingredient silymarin. Silymarin is very effective in treating the liver diseases, hepatitis, and cardiovascular disorders. Since the potential and production speed of this medicinal compound is low in habitats under natural conditions, the use of genetic engineering and production of transgenic plants using the various techniques such as tissue culture has been considered in this plant. The first step in this direction is to obtain an in vitro regeneration protocol. In this study, the leaf explants obtained from the Hungarian cultivar of S. marianum were evaluated for the direct regeneration affected by different combinations of hormones. Six weeks after the culture, the highest shooting (57%) was observed in the ½ MS medium containing 0.4 mg l-1 zeatin, 0.2 mg l-1 BAP, and 0.2 mg l-1 NAA. The shoots were then transferred to the root culture medium with the different hormonal treatments. The results showed that the best treatment to obtain the long and high roots was the ½ MS medium containing 0.25 mg l-1 NAA and 2 g l-1 activated charcoal. The seedlings obtained from the direct regeneration grew well after transplanting into the soil.
M. Borzuee; M. Dejam; M.B. Zahedi
Abstract
Lemonverbena(Lippia citriodora (Palau) Kunth) is a medicinal plant belonging to the Verbenaceae family. Propagation of lemon verbena is commonly performed using stem cutting. In order to study the effects of cutting time (autumn, winter and spring), cutting position (apical, medial and basal) and different ...
Read More
Lemonverbena(Lippia citriodora (Palau) Kunth) is a medicinal plant belonging to the Verbenaceae family. Propagation of lemon verbena is commonly performed using stem cutting. In order to study the effects of cutting time (autumn, winter and spring), cutting position (apical, medial and basal) and different concentrations of indole butyric acid (ABA) (0, 2000 and 4000 ppm) on rooting of stem cuttings of lemon verbena, a factorial experiment based on a three-replicated completely randomized design was performed in a greenhouse in Kavar (Fars province) in 2016-2017. According to the results, the autumn taken cuttings showed better rooting compared with both winter and spring taken cuttings. Regardless of auxin application and cutting time, the highest rooting percentage, root number, root dry weight and shoot length were observed in the basal cuttings of larger diameters. The medial cuttings showed higher rooting percentage than apical ones. The rooting-related characteristics including rooting percentage, root number, and dry weight improved as the concentration of IBA increased up to 2000 ppm. While at the highest concentration of IBA (4000 ppm), most rooting-related characteristics showed no significant increase in comparison to 2000 ppm, the length of the newly emerged shoots on cuttings decreased.
A. Hajiheidar; M. Tohidfar; S.M. Miri; A.R. Zarekarizi; S. Ghadermazi; Kh. Samiee
Abstract
This research was aimed to achieve the optimal protocol for micropropagation of shallot (Allium stipitatum) via basal disc. For regeneration, the basal disc explants were cultured on Murashig & Skoog (MS) medium containing different concentrations of Benzyladenine (BA) (0, 1 & 2 mgl-1) and Naphthalene ...
Read More
This research was aimed to achieve the optimal protocol for micropropagation of shallot (Allium stipitatum) via basal disc. For regeneration, the basal disc explants were cultured on Murashig & Skoog (MS) medium containing different concentrations of Benzyladenine (BA) (0, 1 & 2 mgl-1) and Naphthalene acetic acid (NAA) (0, 0.5 & 1 mgl-1). The regenerated bulblets were rooted on MS and ½MS medium supplemented with different concentrations of BA, NAA, Indole-3-acetic acid (IAA) and activated charcoal (AC). The rooted plantlets were successfully acclimatized under ex vitro conditions. The results indicated that the maximum number of bulblet (12.66) was observed on medium containing 2 mg.l-1 BA and 1 mg.l-1 NAA. In addition, the highest root number (1.77) and length (2.55 cm) were observed on 1 mg.l-1 IAA and 1 g.l-1 activated charcoal and the survival rate was 100% in pots filled with a coco peat: perlite: peat moss (1:2:1 v/v) mixture. This research could be a suitable method for micropropagation of this endangered plant.Hence, the rapid micropropagation of shallot is compulsory because of its various applications and extinction.
H. Sanginabadi; S. Khorasaninejad; Kh. Hemmati; A. Ghasemnejad
Abstract
Lavandula stricta Del. is a native aromatic plant in Iran from Lamiaceae family, traditionally used in the treatment of rheumatic pain, nausea, and flu. In order to investigate the common propagation methods in this plant, two experiments were designed to evaluate the effect of different treatments on ...
Read More
Lavandula stricta Del. is a native aromatic plant in Iran from Lamiaceae family, traditionally used in the treatment of rheumatic pain, nausea, and flu. In order to investigate the common propagation methods in this plant, two experiments were designed to evaluate the effect of different treatments on seed germination and rooting of cuttings at the Gorgan University of Agricultural Sciences and Natural Resources. The first experiment was carried out in a factorial experiment based on a completely randomized design (CRD) with three replications, and two factors were included as follows: Gibberlic acid (0, 250, 500 and 750 ppm) and chilling (chilling at 4 °C for 24 and 48 hours and chilling at -18 °C for 24 and 48 hours). The second experiment was carried out in a factorial experiment based on a complete randomized blocks design with three replications and three factors including indole butyric acid (0 and 250ppm), two types of cutting and three types of medium (cocopeat + perlite, perlite+sand and cocopeat+sand). Then, rooting percentage, root length and root number were measured. According to the results of the first experiment, chilling treatments caused to the reduced germination percentage, seed vigor, plumule and radicle length. In addition, gibberellic acid reduced all seed growth properties but increased the root length. In the second experiment, results showed that the highest rooting (60%) was obtained in the medium containing perlite+sand and 250 ppm ABA.
R. Shahhoseini; M. Moghaddam; D. Kiani; R. Mansori
Abstract
Rosemary (Rosmarinus officinalis L.), belonging to Lamiaceae family, is a perennial, medicinal and aromatic plant. The plant and its secondary metabolites are widely used in landscape designing and pharmaceutical, cosmetic and food industries. This research was conducted as two experiments in a RCBD ...
Read More
Rosemary (Rosmarinus officinalis L.), belonging to Lamiaceae family, is a perennial, medicinal and aromatic plant. The plant and its secondary metabolites are widely used in landscape designing and pharmaceutical, cosmetic and food industries. This research was conducted as two experiments in a RCBD (Randomized Complete Block Design) with six treatments (0, 1000, 2000, 3000, 4000 and 5000 mg l-1) and four replications in order to evaluate the effects of different concentrations of IBA and NAA on rooting of semi-hardwood cuttings of rosemary. Cuttings were treated in solutions of IBA and NAA for 1 min and held in plastic tunnel under mist system. After 70 days, the percentage of rotting, number of roots, average root length, the root fresh and dry weight, length of cutting, leaves and stem fresh and dry weight were measured. The analysis of variance showed that application of IBA and NAA had a significant effect on all the parameters studied. The highest rooting percentage was obtained at concentration of 1000 mg l-1 NAA (84%) and 5000 mg l-1 IBA (66%). The concentration of 4000 mgl-1 IBA was the best treatment on quality and rooting of the semi-hardwood cuttings of rosemary.
F. Alavi Naeini; Z. Asrar; H. Mozafari
Abstract
Rosemary (Rosmarinus officinalis L.) is of great value as an ornamental and medicinal plant. Therefore, studying the growth, morphological and anatomical parameters of rosemary cuttings in rooting stage is of utmost importance. In recent research, the separate and combined effects ...
Read More
Rosemary (Rosmarinus officinalis L.) is of great value as an ornamental and medicinal plant. Therefore, studying the growth, morphological and anatomical parameters of rosemary cuttings in rooting stage is of utmost importance. In recent research, the separate and combined effects of Indole 3- Butyric acid (IBA) and vitamin- B1 (thiamine) on growth, rooting and anatomical structure of adventitious roots in Rosmarinus officinalis cuttings were investigated under hydroponic conditions. A factorial experiment was implemented using nine combined treatments including IBA and vitamin B1 at three levels. The treatments were applied on cuttings for two weeks under standard condition of light, temperature and humidity in hydroponic medium. Then, using cross-section method and double staining, the anatomy of root, in terms of cross-section, cortex cells growth and the density of secondary roots, were studied by a light microscope. Results showed that IBA application with or without B1 significantly affected the growth, anatomy and morphology of rosemary cuttings in rooting stage. However, our results clearly showed that vitamin B1 had an essential role on the growth of roots and IBA stimulated cell division in pericycle, causing the stimulation of rooting response of cuttings. According to he obtained results, the combined use of IBA and vitamin B1 showed significant effect on the propagation of rosemary as an ornamental and medicinal plant; therefore, their application could be recommended in commercial and farm conditions, and even in the case of other ornamental plants having difficulty propagating.
M.H. Assareh; Z. Abravesh; S.R. Tabaei Aghdaei
Abstract
Because of the economic and medicinal importance of Rosa damascena Mill. the propagation of this plant was considered. In order to protect the selected genotypes, this experiment was performed by simple layering method during autumn and winter seasons 2007 on new growth twigsof Rosa damascena mature ...
Read More
Because of the economic and medicinal importance of Rosa damascena Mill. the propagation of this plant was considered. In order to protect the selected genotypes, this experiment was performed by simple layering method during autumn and winter seasons 2007 on new growth twigsof Rosa damascena mature shrubs. The branches were laid in sand bed, soaked for 24 h by different concentrations of IBA and NAA hormones. The results showed that the rate of rooting was high in sand bedding material in autumn and winter. The highest rooting percentage was %100 with 50 mgl-1 concentration of IBA in autumn and winter seasons and the lowest of rooting percentage was %53.33 with 50 mgl-1 concentration of NAA in autumn season. The rooting branches were cut from the parent plants after 3 months and planted in pots containing soil: sand: peatmoss complex (1:1:1) in greenhouse conditions, survival percentage was 90%.
G. Ghazian Tafrishi; M. Azizi; M. Farsi
Volume 22, Issue 3 , November 2006, , Pages 172-179
Abstract
St Johns Wort (Hypericum perforatum L.) from Hypericaceae is an important medicinal plant, which its secondary metabolites, hypericin and hyperforine, have several medicinal effects such as antidepressant, antiviral, antibacterial and etc. The importance of studying in vitro culture of medicinal plants ...
Read More
St Johns Wort (Hypericum perforatum L.) from Hypericaceae is an important medicinal plant, which its secondary metabolites, hypericin and hyperforine, have several medicinal effects such as antidepressant, antiviral, antibacterial and etc. The importance of studying in vitro culture of medicinal plants is optimizing these protocols for subsequent studies about effective factors on biosynthesis of secondary metabolites and applying these methods in improving medicinal plants. Since now there are no report on in vitro culture of Iranian St Johns Wort and for the first time we studied the callogenesis, shoot regeneration and rooting process of this plant. The seeds of Iranian St Johns Wort were collected from Ardebil province and the base growth media was MS and for callogenesis we studied the effect of several levels of 2,4-D (0.25, 0.5, 1, 2 and 5 mg/l) and BA (0.25, 0.5, 1 mg/l) or KIN (0.25, 0.5, 1, 2 and 5 mg/l) and NAA (0.5, 1, 2 and 5 mg/l). Several levels of BA (0.25, 0.5, 1and 5 mg/l) and several levels of KIN (1, 2 and 5 mg/l) accompanied by 1 mg/l NAA were used for shoot regeneration in callus. Several levels of NAA (0, 0.5, 1, 2 and 5 mg/l) were used for rooting of the shoots. The growth condition was 25°c and 16/8 hours period for rooting and shoot regeneration, darkness for callogenesis. The results of callogenesis with Duncans Multiple Range Test at 5% showed that highest callus fresh weight (2.1937 gr) was obtained in 0.25 mg/l 2,4-D with 1 mg/l KIN. Results of shoot regeneration in level 5% showed that maximum number of shoots (95 shoots/call and 4 Cm length) obtained in treatment contain 1 mg/l NAA with 1 mg/l KIN. Results also showed that maximum root number (4.7 roots per shoot and 2.2 Cm length) was in hormone free media.
