Optimizing the regeneration and rooting of Iranian native shallot (Allium stipitatum) via in vitro

Hajiheidar, A.; Tohidfar, M.; Miri, S.M.; Zarekarizi, A.R.; Ghadermazi, S.; Samiee, Kh.

doi:10.22092/ijmapr.2017.106967.1814

Document Type : Research Paper

Authors

¹ M.Sc. Student, Department of Horticulture, Karaj Branch, Islamic Azad University, Karaj, Iran

² Department of Biotechnology, Faculty of Engineering, Energy and New Technology, Shahid Beheshti University, Tehran, Iran

³ Department of Horticulture, Karaj Branch, Islamic Azad University, Karaj, Iran

⁴ Medicinal Plant Research Center, Institute of Medicinal Plants, Academic Center for Education, Culture and Research, Karaj, Iran

https://doi.org/10.22092/ijmapr.2017.106967.1814

Abstract

This research was aimed to achieve the optimal protocol for micropropagation of shallot (Allium stipitatum) via basal disc. For regeneration, the basal disc explants were cultured on Murashig & Skoog (MS) medium containing different concentrations of Benzyladenine (BA) (0, 1 & 2 mgl^-1) and Naphthalene acetic acid (NAA) (0, 0.5 & 1 mgl^-1). The regenerated bulblets were rooted on MS and ½MS medium supplemented with different concentrations of BA, NAA, Indole-3-acetic acid (IAA) and activated charcoal (AC). The rooted plantlets were successfully acclimatized under ex vitro conditions. The results indicated that the maximum number of bulblet (12.66) was observed on medium containing 2 mg.l^-1 BA and 1 mg.l^-1 NAA. In addition, the highest root number (1.77) and length (2.55 cm) were observed on 1 mg.l^-1 IAA and 1 g.l^-1 activated charcoal and the survival rate was 100% in pots filled with a coco peat: perlite: peat moss (1:2:1 v/v) mixture. This research could be a suitable method for micropropagation of this endangered plant.Hence, the rapid micropropagation of shallot is compulsory because of its various applications and extinction.

Keywords

References

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Iranian Journal of Medicinal and Aromatic Plants Research

Optimizing the regeneration and rooting of Iranian native shallot (Allium stipitatum) via in vitro

References

References

Volume 33, Issue 2 - Serial Number 82
June 2017
Pages 244-255

Optimizing the regeneration and rooting of Iranian native shallot (Allium stipitatum) via in vitro

References

References

Volume 33, Issue 2 - Serial Number 82June 2017Pages 244-255

Volume 33, Issue 2 - Serial Number 82
June 2017
Pages 244-255