The effect of vitamin B1 and IBA on rooting and cortex cells growth of adventitious root in Rosmarinus officinalis L. cuttings under hydroponic media

Alavi Naeini, F.; Asrar, Z.; Mozafari, H.

doi:10.22092/ijmapr.2015.12607

Document Type : Research Paper

Authors

¹ Islamic Azad University, Jiroft Branch, Jiroft, Iran

² Department of Biology, Shahid Bahonar University of Kerman and member of HTMRC, Kerman University of medical sciences, Kerman, Iran

³ Ph.D. Student, Department of Biology, Shahid Bahonar University of Kerman, Kerman, Iran

https://doi.org/10.22092/ijmapr.2015.12607

Abstract

Rosemary (Rosmarinus officinalis L.) is of great value as an ornamental and medicinal plant. Therefore, studying the growth, morphological and anatomical parameters of rosemary cuttings in rooting stage is of utmost importance. In recent research, the separate and combined effects of Indole 3- Butyric acid (IBA) and vitamin- B₁ (thiamine) on growth, rooting and anatomical structure of adventitious roots in Rosmarinus officinalis cuttings were investigated under hydroponic conditions. A factorial experiment was implemented using nine combined treatments including IBA and vitamin B₁ at three levels. The treatments were applied on cuttings for two weeks under standard condition of light, temperature and humidity in hydroponic medium. Then, using cross-section method and double staining, the anatomy of root, in terms of cross-section, cortex cells growth and the density of secondary roots, were studied by a light microscope. Results showed that IBA application with or without B₁ significantly affected the growth, anatomy and morphology of rosemary cuttings in rooting stage. However, our results clearly showed that vitamin B₁ had an essential role on the growth of roots and IBA stimulated cell division in pericycle, causing the stimulation of rooting response of cuttings. According to he obtained results, the combined use of IBA and vitamin B₁ showed significant effect on the propagation of rosemary as an ornamental and medicinal plant; therefore, their application could be recommended in commercial and farm conditions, and even in the case of other ornamental plants having difficulty propagating.

Keywords

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Iranian Journal of Medicinal and Aromatic Plants Research

The effect of vitamin B1 and IBA on rooting and cortex cells growth of adventitious root in Rosmarinus officinalis L. cuttings under hydroponic media

References

References

Volume 31, Issue 1 - Serial Number 69
April 2015
Pages 16-30

The effect of vitamin B1 and IBA on rooting and cortex cells growth of adventitious root in Rosmarinus officinalis L. cuttings under hydroponic media

References

References

Volume 31, Issue 1 - Serial Number 69April 2015Pages 16-30

Volume 31, Issue 1 - Serial Number 69
April 2015
Pages 16-30