F. Oroojalian; R. Kasra-Kermanshahi; M. Azizi; M.R. Bassami
Abstract
Essential oils of three medicinal plants species, including Carum copticum (L.) C. B. Clarke, Bunium persicum (Boiss.) B. Fedtsch. and Cuminum cyminum L., were obtained by hydrodistillation and their constituents were analyzed by GC and GC/MS using retention indices and fragmentation patterns. The antibacterial ...
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Essential oils of three medicinal plants species, including Carum copticum (L.) C. B. Clarke, Bunium persicum (Boiss.) B. Fedtsch. and Cuminum cyminum L., were obtained by hydrodistillation and their constituents were analyzed by GC and GC/MS using retention indices and fragmentation patterns. The antibacterial effects (MIC and MBC) of the essential oils were assessed on several food-borne pathogens, namely Staphylococcus aureus, Bacillus cereus, Escherichia coli O157: H7, Salmonella enteritidis and Listeria monocytogenes by microdilution technique using ELISA reader. Because of the combinatory usage of Bunium persicum and Cuminum cyminum in folk medicine, the affect of essential oil on food organoleptic properties and MIC values of the plants, interaction of Bunium persicum and Cuminum cyminum essential oils were also studied by FICindex determination using modified dilution checkboard method. The results showed that there are noticeable differences between the essential oils as their constituents as concerned, while ρ-cymen and γ-terpinene detected in all essential oils in different percentage. The main components of essential oils of C. copticum were thymol (48.4%), ρ-cymene(21.8%) and γ-terpinene (21.3%). The major constituents of B. persicum were γ-terpinene (44.2%), cuminaldehyde (16.9%), γ-terpinen-7-al (10.5%), and ρ-cymen (8%) while those of C. cyminum were cuminaldehyde (30.2%), ρ-cymene(14.1%), γ-terpinene (12.8%), and safranal (9.4%). The ranges of minimum inhibitory concentrationof the oils were 0.03-0.5,0.18-3.0, and 0.37-3.0 mg/ml, respectively, for C. copticum, B. persicum and C. cyminum. Moreover, the combination of B. persicum and C. cyminum essential oils confirmed synergistic and additive activities against the pathogens. In conclusion, although the MIC of Bunium persicum and Cuminum cyminum essential oils were lower than C. copticum, but combinatory usage of these essential oils especially against gram-positive bacteria produced promissing results. So application of these essential oils is recommended in combination as an efficients and complementary method for control of food borne pathogens with lower side effects on organolpetic properties of food.
S.H. Hejazian; A. Fattahi Bafghi; S.M. Mahdavi
Volume 25, Issue 1 , May 2009, , Pages 104-112
Abstract
One of the important aims of biological investigations is finding substances which are involved in relieving pain. Application of medicinal herbs for relieving pain is very useful. Carum copticum (L.) C. B. Clarke from Umbelliferae family has been introduced as an analgesic agent in Iranian traditional ...
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One of the important aims of biological investigations is finding substances which are involved in relieving pain. Application of medicinal herbs for relieving pain is very useful. Carum copticum (L.) C. B. Clarke from Umbelliferae family has been introduced as an analgesic agent in Iranian traditional literature which is widely used for relieving neuropathy pain and headache. This study was conducted to investigate the analgesic effect of Carum copticum fruit on formalin induced pain as compared with oil part of Carum copticum. Formalin test was used as a standard pain inducing test and the analgesic effect of intra peritoneal injection of 20ml/kg of Carum copticum extract were evaluated. Our findings showed that aqueous part of Carum copticum extract had no effect during one hour formalin test as compared with control group (P>0.05). Therefore, due to the analgesic effect of essential fatty acids and its crude extract of Carum copticum, the non analgesia property might be related to absent of oil part.
S.H. Hejazian; M.H. Dashti; A. Salami
Abstract
One of the important objectives of biological investigations is to find substances which are involved in relieving pain. In this regard, application of medicinal herb are very useful. Carum copticum (L.) C. B. Clarke from Umbelliferae family has been introduced as an analgesic agent in Iranian traditional ...
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One of the important objectives of biological investigations is to find substances which are involved in relieving pain. In this regard, application of medicinal herb are very useful. Carum copticum (L.) C. B. Clarke from Umbelliferae family has been introduced as an analgesic agent in Iranian traditional literature and it is widely used to relieve neuropathic pain and headache. In the present study we conducted to investigate the analgesic effect of Carum copticum fruit on formalin induced pain as compared with morphine. In this study formalin test was used as a standard pain inducing test and the analgesic effect of intra peritoneal injection of 400mg/kg of Carum copticum extract were compared with different concentrations of morphine (1&2mg/kg). The findings show that Carum copticum has no effect on early response of pain but its effect on late phase of formalin test in chronic response of pain is significant (P=0.01). This effect was the same as 1mg/kg of morphine sulfate. According to our finding Carum copticum extract significantly reduced pain sensation in inflammatory phase of formalin test. This may be due to its essential oil which has been reported to have an analgesic and inflammatory effect.
