H. Mirzaian; F. Ghaffarifar; J. Sadraei; A. Abdoli
Abstract
Toxoplasmosis is one of the most common zoonotic infections worldwide. The aim of this study was to evaluate anti-Toxoplasma gondii activities of aqueous and alcoholic extracts of Myrtus communis L. in vitro. In this study, effects of aqueous and alcoholic extracts of M. communis on T. gondii-infected ...
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Toxoplasmosis is one of the most common zoonotic infections worldwide. The aim of this study was to evaluate anti-Toxoplasma gondii activities of aqueous and alcoholic extracts of Myrtus communis L. in vitro. In this study, effects of aqueous and alcoholic extracts of M. communis on T. gondii-infected mouse macrophages were evaluated by MTT and direct method. The results showed that the aqueous extract of M. communis with concentrations of 12.5 and 25 µg/ml had the highest anti-T. gondii activity (87 and 81%), while their lethal effect on non-infected macrophage was less than other concentrations. The present study showed that the aqueous and alcoholic extracts of M. communis have a considerable inhibitory effect on Toxoplasma gondii tachyzoites and parasite intracellular proliferation in vitro.
F. Senfi; M.H. Safaralizadeh; S.A. Safavi; Sh. Aramideh
Abstract
Nowadays, the application of plant essential oils has been highly considered as an alternative for synthetic insecticides especially in stored-product pests. One of the potential resources for development of new pesticides is plant materials, acting as repellents, antifeedants, oviposition deterrents ...
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Nowadays, the application of plant essential oils has been highly considered as an alternative for synthetic insecticides especially in stored-product pests. One of the potential resources for development of new pesticides is plant materials, acting as repellents, antifeedants, oviposition deterrents and toxic compounds. In this study, fumigant toxicity of essential oils extracted from Laurus nobilis L. and Myrtus communis L. was assessed on adults and eggs stage of Callosobruchus maculatus. This research was conducted in a completely randomized design at 30 ± 2°C and relative humidity of 60 ± 5 % in darkness. Each essential oil was tested in 3 replications and 5 concentrations. The LC50 values for L. nobilis and M. communis for adults of the beetle were calculated to be 71.782 and 44.138 micro liter per liter of air and the LC95 values were calculated to be 216.278 and 141.716 micro liter per liter of air, respectively. The LC50 values for L. nobilis and M. communis for eggs of the beetle were calculated to be 63.994 and 38.371 microliter per liter of air, respectively. It seems that essential oils can be used as a low-risk insecticide.
M.H. Yazdi; M.R. Pourmand; M. Bayat; A. Shahinjafari
Abstract
In order to investigate the antimicrobial effects of Iranian herbal essences of Zataria multiflora Boiss., Myrtus communis L. and Eucalyptus officinalis, this research was conducted under CLSI guidelines in vitro situation. Since Streptococcus pneumoniae, Moraxella catarrhalis and Haemophilus influenzae ...
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In order to investigate the antimicrobial effects of Iranian herbal essences of Zataria multiflora Boiss., Myrtus communis L. and Eucalyptus officinalis, this research was conducted under CLSI guidelines in vitro situation. Since Streptococcus pneumoniae, Moraxella catarrhalis and Haemophilus influenzae are highly involved in Sinusitis and Bronchitis, the antibacterial effects of the essences were investigated on these species. Antibiotic susceptibility testing was performed by disk diffusion method for the essential oils and of determination Minimum Inhibitory Concentration (MIC) was carried out by serial dilutions. Zataria multiflora was effective against all the tested bacterias e.g. Streptococcus pneumoniae (52mm), Moraxella catarrhalis (55mm) and Haemophilus influenzae (40mm) based on disk diffusion method. Furthermore, the highly effective inhibition of Myrtus communis was on Moraxella catarrhalis (50 mm) (MIC≥78.66 µg/ml). This study showed that the herbal essences are very active against Streptococcus pneumoniae, Moraxella catarrhalis and Haemophilus influenzae in vitro situation. This confirms the application of herbal medicines for treating a range of infectious diseases in ancient times.
M. Makkizadeh; R. Farhoudi; H. Naghdi badi; A. Mehdizadeh
Abstract
This study has been conducted to overcoming seed dormancy of Rubia tinctourum L., Echinacea angustifolia D.C. and Myrtus communis L.. Treatments to break seed dormancy in Rubia tinctourum included: untreated seed (control), mechanical scarification with sandpaper, imbibitions in hot water at 70°C ...
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This study has been conducted to overcoming seed dormancy of Rubia tinctourum L., Echinacea angustifolia D.C. and Myrtus communis L.. Treatments to break seed dormancy in Rubia tinctourum included: untreated seed (control), mechanical scarification with sandpaper, imbibitions in hot water at 70°C and 90°C for 5 and 10 minutes, chemical scarification with concentrated sulfuric acid for 10, 15 or 20 minutes, pre-chilling (4°C) for 2, 4 or 6 weeks, soaking in gibberellic acid (500ppm) and continuous light. Treatments to break seed dormancy in Echinacea angustifolia included: untreated seed (control), soaking in gibberellic acid (250 and 500 ppm), continuous light, Potassium Nitrate (0/3%), pre-chilling (4°C) for 4, 7 or 10 weeks, continuous light and combined treatment (soaking in gibberellic acid (250ppm) and pre-chilling for 4 week). Treatments to break seed dormancy in Myrtus communis included: untreated seed (control), mechanical scarification with blade and sandpaper, chemical scarification with concentrated sulfuric acid for 3 minutes, pre-chilling (4°C) for 7 or 10 weeks and soaking in gibberellic acid (250 and 500ppm). According to results in R. tinctourum, sulfuric acid for 15 minutes was significantly efficient in promoting germination. From the above one can infer that dormancy of the seeds of R. tinctourum was probably associated with the seeds coat, since the treatment that induce germination were those that can effect disruption of the seed coat. This experiment also showed that combined treatment significantly increased E. angustifolia seed germination. As stratification and GA3 had main role on breaking of seed dormancy so it is known that dormancy is physiological and it is related to embryo and inhibitor factor or both of them. Results indicated that germination of M. communis seeds mechanically scratched with blade significantly increased. So, reason of seed dormancy is hard coated seeds. The seed coat is as one physical barrier against growth of embryo or radicle that inhibits in absorption of water and gas-exchanges. Therefore, type of dormancy is initial dormancy (induced dormancy).
