E. Taghian; N. Saidi; F. Sefidkon; H. Saderi; I. Rasooli; R. Mohammad Salehi; P. Owlia
Abstract
Treatment of Staphylococcus aureus (S. aureus) infection is a problematic subject in the world. The aim of this study was an evaluation of the antimicrobial effect of four species of Satureja essential oils on biofilm formation and hemolysin production in S. aureus. MIC of Satureja essential oils (Satureja ...
Read More
Treatment of Staphylococcus aureus (S. aureus) infection is a problematic subject in the world. The aim of this study was an evaluation of the antimicrobial effect of four species of Satureja essential oils on biofilm formation and hemolysin production in S. aureus. MIC of Satureja essential oils (Satureja khuzistanica Jamzad, Satureja bachtiarica Bunge, Satureja rechingeri Jamzad, Satureja mutica Fisch. & C. A. Mey.) were determined by micro dilution broth method against standard strains of S. aureus ATCC 29213(MSSA) & ATCC 33591(MRSA). Sub- MICs of Satureja essential oils were used to assay biofilm formation and hemolysin production. The results showed that all essential oils had antimicrobial effect against standard strains of S. aureus. In the presence of sub- MICs of essential oils, biofilm formation and hemolysin production were significantly reduced. The results show the potent antimicrobial effects of Saturejaessential oils against S. aureus and more study is recommended to use it in controlling S. aureus infections.
M. Azizkhani; R. Aznar; P. Elizaquivel
Abstract
Staphylococcal food poisoning is resulted from the consumption of a food in which enterotoxigenic staphylococci have grown and produced toxins. Targeting bacterial virulence factors is now gaining interest as an alternative strategy to develop new types of antimicrobial preservatives. The purpose of ...
Read More
Staphylococcal food poisoning is resulted from the consumption of a food in which enterotoxigenic staphylococci have grown and produced toxins. Targeting bacterial virulence factors is now gaining interest as an alternative strategy to develop new types of antimicrobial preservatives. The purpose of this study was to determine the effect of R. officianalis L. (Ros) essential oil (EO) on growth and gene expression of enterotoxins A, C, and E in Staphylococcus aureus ATCC 29213. In this study, after determining the minimum inhibitory concentration, the growth and secretion of SEA, SEC, and SEE (detected by ELISA method) by S. aureus treated with graded subinhibitory concentrations of EO was evaluated. In addition, the influence of the EO on the transcription of sea, sec and see (the genes encoding SEA, SEC and SEE, respectively) was analyzed by quantitative RT-PCR. Ros EO at a concentration of 75% MIC, significantly, reduced the growth of S. aureus. EO inhibited the transcription of sea, sec and see in S. aureus, in a dose-dependent manner, resulting in a reduction of SEA, SEC and SEE secretion. These data suggest that the Ros EO may be useful as a natural preservative against the growth and enterotoxin production of S.aureus in food industry.
T. Dadgar; E. Ghaemi; M. Asmar; M. Mazandarani; M. Bazueri
Volume 23, Issue 1 , May 2007, , Pages 73-85
Abstract
Staphylococcu aureus is an important cause of community and hospital acquired infections. Infections caused by methicillin resistant S. aureus (MRSA) are mainly nosocomial and are increasing in all around the world. Many attempts have been made by the researchers to find new compounds as ...
Read More
Staphylococcu aureus is an important cause of community and hospital acquired infections. Infections caused by methicillin resistant S. aureus (MRSA) are mainly nosocomial and are increasing in all around the world. Many attempts have been made by the researchers to find new compounds as an appropriate substitute for these antibiotics. In this study antimicrobial activities of aqueous and boilling extracts of 6 medicinal plants species collected from Golestan province on clinical and standard strains of MRSA and MSSA were assessed by disc diffusion method. Each test was repeated 3 times and mean inhibition zone was recorded. The boilling and aqueous extracts of 5 and 3 plants showed the best anti staphylococcal effect, respectively. The boilling extract of Artemisia sieberi, Punica granatum, which the maximum inhibition zone was 16.6 mm. raised the most outstanding in vitro antibacterial activities. As a result boilling extracts had better antibacterial effects than aqueous extract.
H. Saderi; P. Owlia; M. Radmanesh
Volume 22, Issue 4 , February 2007, , Pages 366-372
Abstract
Staphylococcus aureus is one of the common bacteria in human infection. Increasing antibiotic resistance in Staphylococcus aureus lead to vast usage of antibiotics and releasing new drugs to market. In this respect, medical herbs with antimicrobial effect have had important role in traditional medicine. ...
Read More
Staphylococcus aureus is one of the common bacteria in human infection. Increasing antibiotic resistance in Staphylococcus aureus lead to vast usage of antibiotics and releasing new drugs to market. In this respect, medical herbs with antimicrobial effect have had important role in traditional medicine. The purpose of this study was evaluating antimicrobial effect of hydroalcoholic and aqueous extracts of seed and stem of Ruta graveolens plant on Staphylococcus aureus (ATCC 25923). Agar diffusion method was used and relative MIC and MBC of effective extracts were determined with macro dilution method. The change of number of bacteria in the presence of extract with concentration equal to MBC was also determined for different period of times. The results showed that unlike aqueous extract, the hydroalcoholic extracts of Ruta graveolens have no effect on Staphylococcus aureus. MIC and MBC of aqueous extract of stem were 10% and 20%, whereas, aqueous extract of seed were measured 10% and >30%, respectively. In the presence of aqueous extract of stem of Ruta graveolens, bacterial population was reduced gradually. Since Ruta graveolens is a native and abundant plant species in Iran; it is likely to produce drugs against Staphylococcus aureus infection which require further research.
P. Owlia; H. Saderi; F. Matloob; M.B. Rezaee
Abstract
Staphylococcus aureus is an important pathogen that produces widespread infections. Increasing antibiotic usage for S. aureus infections, created antibiotic resistance and hence need for production of new antibiotics. Medicinal herb with antimicrobial effect have important role in traditional medicine. ...
Read More
Staphylococcus aureus is an important pathogen that produces widespread infections. Increasing antibiotic usage for S. aureus infections, created antibiotic resistance and hence need for production of new antibiotics. Medicinal herb with antimicrobial effect have important role in traditional medicine. The aim of this study was to survey antimicrobial effect of hydroalchoholic extract of Zataria multiflora on S. aureus. The minimal inhibitory concentration (MIC) and minimal bactericidal concentratioin (MBC) of extract of dried leaf of Z. multiflora were determined by macro-dilution method. The changes of the number of bacteria in the presence of different concentrations (25% and 30%) of extract and time were assessed. The results were compared with changes of the number of bacteria in presence of 4 µg/mloxacillin. The results showed that MIC and MBC was equal 25% for extract of Z. multiflora and reduction of the number of bacteria on the present of 30% of extract after 8h is equal with reduction of the number of bacteria exposed to 4 µg/ml oxacillin after 4h. Z .multiflora is native for Iran. Further studies and research on the antimicrobial effects of this medicinal herb, probably, may lead to production of herbal drugs.
Gh. Goudarzi; M. Sattari; M. Goudarzi; M. Bigdeli
Abstract
With a view to occurrence of drug resistance, attempts have been made to arrive at new compounds of plant origin as substitute antibiotics. Dried chamomile flowers were added to 85% ethanol and distillated water separately and were then distilled. Dried weights of the extracts were determined per ml. ...
Read More
With a view to occurrence of drug resistance, attempts have been made to arrive at new compounds of plant origin as substitute antibiotics. Dried chamomile flowers were added to 85% ethanol and distillated water separately and were then distilled. Dried weights of the extracts were determined per ml. The anti bacterial properties of the extracts were investigated by tube dilution method in broth media. Different concentrations of the extracts were used to determine minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). Equal amounts of these concentrations were added to wells in Muller – Hinton agar. Mean diameter of growth inhibition zones (mm) were noted. Some concentrations of the alcoholic extract showed significant antibacterial effects. Alcoholic extract at 2.617 mg/ml concentration was growth inhibitory and at 5.237 mg/ml concentration was bactericidal on both strains whereas; the aquatic extract did not show any antimicrobial effect. So the alcoholic extract of German chamomile showed antibacterial activity on the Staphylococcus aureus strains; however it’s introduction as an antibacterial compound require further investigations.
