Document Type : Research Paper
Authors
Bacteriology Department, Medicine Faculty, Tarbiat Modarres University, Tehran, Iran
Abstract
With a view to occurrence of drug resistance, attempts have been made to arrive at new compounds of plant origin as substitute antibiotics.
Dried chamomile flowers were added to 85% ethanol and distillated water separately and were then distilled. Dried weights of the extracts were determined per ml. The anti bacterial properties of the extracts were investigated by tube dilution method in broth media. Different concentrations of the extracts were used to determine minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). Equal amounts of these concentrations were added to wells in Muller – Hinton agar. Mean diameter of growth inhibition zones (mm) were noted.
Some concentrations of the alcoholic extract showed significant antibacterial effects. Alcoholic extract at 2.617 mg/ml concentration was growth inhibitory and at 5.237 mg/ml concentration was bactericidal on both strains whereas; the aquatic extract did not show any antimicrobial effect.
So the alcoholic extract of German chamomile showed antibacterial activity on the Staphylococcus aureus strains; however it’s introduction as an antibacterial compound require further investigations.
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