Biotechnology
S.M. Mostafavi; M.R. Abdollahi; D. Dastan; H. Sarikhani
Abstract
Caper (Capparis spinosa L.) is a rich source of rutin, plays an essential role in human health. In the present study, the effects of cold (25°C as control, 4°C, and 7°C for 2, 4, and 7 days), heat (25°C as control, 30°C for 14 days, 32°C for 2 and 4 days, and 35°C for 8 hours), ...
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Caper (Capparis spinosa L.) is a rich source of rutin, plays an essential role in human health. In the present study, the effects of cold (25°C as control, 4°C, and 7°C for 2, 4, and 7 days), heat (25°C as control, 30°C for 14 days, 32°C for 2 and 4 days, and 35°C for 8 hours), and carbohydrate treatments on the androgenesis efficiency were studied in the anther culture of caper. Also, the effects of maltose and sucrose at the concentrations of 30 and 60 g L-1 in combination with two temperature treatments (1- 30°C for 14 days and 2- 7°C for 7 days + azacytidine and 2,4-D pretreatments) on the androgenesis induction was evaluated. The temperature and carbohydrate treatments showed statistically significant differences (p < /em>≤0.01) in terms of callus and embryo formation. The 7°C for 2, 4, and 7 days produced the highest percentage (at the third week: 80, 78.34, and 76.67%, respectively) and callogenesis speed (7.85, 7.75, and 7.60 calli week-1, respectively) and the 7°C for 7 days produced the highest embryo production (0.57 embryo anther-1). The 30°C for 14 days treatment showed the highest percentage (at the third week: 100%) and callogenesis speed (9.44 calli week-1). While the 32°C for 2 and 3 days and also 30°C for 14 days produced the highest number of embryos per anther (0.22, 0.20, and 0.18 embryo, respectively). The use of 30 g L-1 maltose in combination with the 30°C for 14 days produced the highest percentage (at the third week: 91.66%) and callogenesis speed (8.94 calli week-1), while the 30 g L-1 maltose in combination with the 7°C for 7 days + azacytidine and 2,4-D pretreatments produced the highest mean embryo number per anther (0.55 embryo). The results of this research are of great importance for the use in the caper breeding programs.
M. Dahmardeh; R. Taji; I. Khammari; T. Hadadi
Abstract
In order to investigate the effects of iron and silver nanoparticles on morphological and physiological traits of borage (Borago officinalis L.), an experiment was conducted as factorial based on randomized complete block design with three replications in 2016 at the University of Zabol. The ...
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In order to investigate the effects of iron and silver nanoparticles on morphological and physiological traits of borage (Borago officinalis L.), an experiment was conducted as factorial based on randomized complete block design with three replications in 2016 at the University of Zabol. The identification of synthesized nanoparticles was performed using FT-IR infrared spectroscopy and scanning electron microscopy. The treatments included iron and silver nanoparticles with four levels (control, 40, 60, 80 ppm), sprayed together and separately in two stages. The results indicated that spraying iron and silver nanoparticles had significantly different effects on soluble carbohydrates, mucilage, chlorophyll a, carotenoids, leaf and branch number, inflorescence height, flower number, and dry flower weight. Iron and silver nanoparticles (80ppm) had the highest effect on the traits measured. In all traits measured, iron nanoparticles were more effective than silver nanoparticles except chlorophyll b. Simultaneous use of iron and silver nanoparticles showed different effects on all traits, so that the highest significant difference was recorded for iron and silver nanoparticles at 80ppm.
A. Afkari
Abstract
In order to investigate the effects of drought stress and nitrogen fertilizer on some physiological and biochemical characteristics of basil (Ocimum basilicum L.), a split-plot experiment was conducted based on randomized complete block design in three replications in the research farm of Islamic Azad ...
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In order to investigate the effects of drought stress and nitrogen fertilizer on some physiological and biochemical characteristics of basil (Ocimum basilicum L.), a split-plot experiment was conducted based on randomized complete block design in three replications in the research farm of Islamic Azad University, ArdabilBranch, 2015. The treatments included drought stress (70, 140 and 210 mm of class A evaporation pan) and nitrogen fertilizer at three levels (0, 50 and 100 kg/hectare) respectively. The results showed that the effect of drought stress treatment was significant on the traits investigated. The interaction of drought stress × nitrogen fertilizer had a significant effect on the traits measured, such as chlorophyll a, total chlorophyll, relative water content, dry matter yield, and essential oil yield. In addition, fertilizer treatment caused a significant increase in the physiological characteristics, dry matter yield, and essential oil yield. According to the results, the maximum content of chlorophyll a (2.65 mg/g fresh weight), total chlorophyll (3.42 mg/g fresh weight), relative water content (72.02 percent), dry matter yield (2261.25 kg/hectare), and essential oil yield (7.13 kg/hectare) were obtained for the control treatment and 100 kg of nitrogen per hectare. The maximum content of carbohydrates (3.40 μg/g glucose per fresh weight) and proline (0.681 µmol/g fresh weight) was related to the severe stress treatment. The content of chlorophyll a, b, total, relative water content, carotenoid, and essential oil percent and yield decreased by increasing the drought stress, while the content of carbohydrate and proline increased. Therefore, irrigation at 70 mm evaporation and 100 kg nitrogen fertilizer per hectare could be recommended as the best treatment to achieve the highest levels of chlorophyll a, chlorophyll total, relative water content, dry matter yield, and essential oil yield of basil.
K. Saeedi; R. Omidbaigi
Abstract
Dog rose (Rosa canina L.) is considered as one of the most important medicinal plants, which contain valuable medicinal and nutritional compounds in its fruits. In order to determine total phenolic, soluble carbohydrates, carotenoid contents and minerals in dog rose fruits collected from five different ...
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Dog rose (Rosa canina L.) is considered as one of the most important medicinal plants, which contain valuable medicinal and nutritional compounds in its fruits. In order to determine total phenolic, soluble carbohydrates, carotenoid contents and minerals in dog rose fruits collected from five different ecological regions in south-west of Iran (Kiar and Gerd Bishe in Charmahal & Bakhtiari, Yasuj and Meymand in Kohgiluye & Boyer Ahmad and Semirom in Isfahan), an evaluation was carried out during 2006-2007. Total phenolic contents, total soluble carbohydrates and total carotenoids varied from 83.13-94.14 mg GAE/g DW, 13.34–17.14% and 408.36–495 µg/g FW, respectively. The N, P, K, Mg, Ca, Fe, Zn and Mn values of fruit samples differed from 0.73-1.15%, 2816–4278 ppm, 2036–3325 ppm, 689–1092 ppm, 821–1243 ppm, 34–52 ppm, 15–29 ppm and 23–43 ppm, respectively. This study showed that fruits of dog rose are a rich source of phenolic contents, soluble carbohydrates, carotenoids and minerals. In this study, content of active substances affected by climatic factors of case studies was investigated.
M. Naderi Hagybaghercandy; M.B. Rezaee; K. Jaimand
Volume 19, Issue 2 , August 2003, , Pages 149-156
Abstract
Gum arabic or gum acacia is oldest and best known of all the natural gums. Gum arabic is one of carbohydrat`s gum group that is nutritive and demulcent, and exerts a soothing influence upon irritated or inflamed mucous tissues, by shielding them from the influence of deleterrious agents, atmospheric ...
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Gum arabic or gum acacia is oldest and best known of all the natural gums. Gum arabic is one of carbohydrat`s gum group that is nutritive and demulcent, and exerts a soothing influence upon irritated or inflamed mucous tissues, by shielding them from the influence of deleterrious agents, atmospheric air, etc In this investigation gum of acacia nilotica plant collected from farm of medicinal plant Research in Booshehr province for study quantitative and qualitative. In this study quantitative, determination of carbohydrate has done by Antron method by spectrophotometry (HITACHI 340 ) in 625 nm. The percent of total carbohydrates in gum arabic is 6.51 %. Also in this study qualitative specified that there are pentoses and hexoses and uronic acid in gum arabic by TLC method. In this method used– methylethylketone several reagentes and solventes that best is formic acid – tertiary butanol – water and anisidine – phthalic acid – ethanol.
