Agriculture and horticulture
Farhad Bagheri fard sharabiani; Esmaeil Chamani; Mousa Torabi Giglou; Alireza Ghanbari; Younes Pourbeyrami hir
Abstract
Background and objectives: The beneficial effects of the chamomile plant have been attributed to its essential oil, so any factor that affects the quantity of its essential oil will be of interest to researchers. To investigate the effect of plant growth regulators salicylic ...
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Background and objectives: The beneficial effects of the chamomile plant have been attributed to its essential oil, so any factor that affects the quantity of its essential oil will be of interest to researchers. To investigate the effect of plant growth regulators salicylic acid and methyl jasmonate on the production of secondary metabolites in chamomile plants, this experiment was conducted under the native in vitro conditions of Sharbian City.Methodology: To conduct this research, chamomile seeds were collected from Sharbian (N ʺ52 58 37 and E ʺ06 ʹ11 ˚), East Azerbaijan province. After transfer, the seeds were sterilized for 15 minutes in a detergent. Their surfaces were disinfected with 70% alcohol for 45 seconds and then with sodium hypochlorite for 30 minutes. After surface disinfection, they were washed twice with distilled water. The basic culture medium in this study was the MS culture medium. All cultures were placed in the growth chamber at a temperature of 24±2°C and 16 hours of light and 8 hours of darkness. A factorial experiment was performed in a complete random design with three replications. Test treatments included salicylic acid (SA) and methyl jasmonate (MeJA) at five levels (0, 50, 100, 200, 400 μM). Minguez-Mosquera and Perez-Galvez methods were applied to determine photosynthetic pigment amounts. The amount of phenolic compounds in the plant extract was measured with a slight modification based on the Slinkard and Singleton method with the Folin-Ciocalteau reagent. The data related to this research were analyzed using SAS V.9.g.1 statistical software. The comparison of treatment averages was done with Duncan's test at 5% and 1%. Graphs were drawn using Microsoft Excel software.Results: The analysis of variance showed that the interaction effect of the applied treatments on the traits of stem weight, root weight, stem length, and root length is significant at the 1% probability level. In addition, it is significant on the number of stems at the 5% probability level. The comparison of the averages showed that the highest amount of stem weight was obtained in the interaction effect of (50 µM) SA and (200 µM) MeJA, and the lowest amount of stem weight was obtained in the interaction effect of (400 µM) SA and (400 µM) MeJA treatment. The comparison of the means shows a significant difference in this trait. The highest amount of root weight was obtained in the interaction effect of (100 µM) SA and (100 µM) MeJA, and the lowest amount of root weight was obtained in the interaction of (400 µM) SA and (400 µM) MeJA treatment. Came. A comparison of the averages showed that the highest number of stems was obtained in the interaction of (100 µM) SA and (50 µM) MeJA, and the lowest number of stems was obtained in the interaction of (400 µM) SA and (200 µM) MeJA. The comparison of the means showed the significance of the stem and root length traits. The highest value of these two traits was due to the interaction of (50 µM) SA and (100 µM) MeJA treatments, and the lowest value of stem length was in the combination of (400 µM) SA and (µM) treatments. 400) MeJA and root length were measured from the combination of SA (400 µM) and MeJA (200 µM) treatments. In the biochemical traits, variance analysis of the data showed that the effect of SA levels on the amount of chlorophyll a, b, carotenoid, total chlorophyll, phenol, and flavonoid in all three wavelengths was significant at the 1% probability level.Conclusion: In this experiment, it was shown that metabolite production depends not only on enzyme activity but also on stimulant concentration. Increasing the stimulus more than usual not only increases metabolism but also reduces or stops the production of metabolites through the reduction of enzyme activity (probably through the reduction of the corresponding gene expression).
Improvement and breeding
B. Asghari; S. Mafakheri; M. M. Zarrabi
Abstract
Background and Objectives: Hibiscus sabdariffa L., commonly known as Roselle, is a plant cultivated in many regions of the world regions. Roselle has been used as a medicinal herb in traditional medicine for controlling and treating different diseases and physical ailments. These therapeutic effects ...
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Background and Objectives: Hibiscus sabdariffa L., commonly known as Roselle, is a plant cultivated in many regions of the world regions. Roselle has been used as a medicinal herb in traditional medicine for controlling and treating different diseases and physical ailments. These therapeutic effects are attributed to active compounds such as carotenoids, fatty acids, and phenolic compounds, including phenolic acids and flavonoids. One common method to stimulate plants for increased production of active compounds is subjecting them to various types of stresses, such as drought or salinity, along with plant growth regulators. This study investigated salicylic acid's effect on the phytochemical content and various biological properties of Roselle plants under water scarcity conditions.Methodology: The experiment was conducted as a factorial experiment in a completely randomized design, including irrigation intervals at three levels: three days (D1), five days (D2), and seven days (D3), and foliar application of salicylic acid at three levels: 0 (SA0), 0.75 mM (SA1), and 1.25 mM (SA2), with three replicates in a research greenhouse at the International Imam Khomeini University. The required seeds for cultivation were obtained from Pakanbazr Company in Isfahan. The imposition of drought stress through irrigation intervals of 3, 5, and 7 days started at the 4-leaf stage of the plants, and the plant spraying with salicylic acid was performed at the 6-leaf stage, three days before the start of irrigation treatments. The measurement of electrolyte leakage and malondialdehyde content was carried out to determine membrane stability and lipid peroxidation level. Additionally, the measurement of proline content and the activity of three antioxidant enzymes, including peroxidase, catalase, and ascorbate peroxidase, was performed in different samples. Furthermore, the total phenolic, flavonoid, and anthocyanin contents, as the main secondary metabolites of Roselle plants, were measured. The biological and medicinal properties, including the free radical scavenging activity (DPPH) and α-glucosidase inhibitory activity, were also evaluated. The obtained data were analyzed using SAS statistical software version 9.4. Mean comparisons were conducted using Duncan's multiple range test at a significance level of 5%.Results: The results showed both electrolyte leakage and malondialdehyde content increased significantly with increasing levels of applied drought stress on Roselle plants. However, overall, applying salicylic acid treatments led to a decrease in both indicators. The measurement of proline content in Roselle samples showed that the highest amount of proline was observed in plants subjected to the highest drought stress and treated with the 1.5 mM salicylic acid solution. The activity of all three antioxidant enzymes increased with the intensity of the applied drought stress and the concentration of salicylic acid solutions. Roselle's highest levels of phenolic and anthocyanin content were observed in samples sprayed with 1.5 mM salicylic acid solution. The change in anthocyanin content of Roselle plants also demonstrated that increasing the severity of water scarcity resulted in higher levels of this compound. The highest DPPH free radical scavenging activity was observed in Roselle samples grown under the highest drought severity and sprayed with the highest concentration of salicylic acid (61.93%). The highest antioxidant activity was also observed in samples treated with the highest concentration of salicylic acid solution in all irrigation regimes. The highest α-glucosidase inhibitory activity (69.2% and 67.7%) was associated with Roselle samples grown under the highest applied drought severity, sprayed with 1.5 mM and 0.75 mM salicylic acid solutions, respectively.Conclusion: It can be concluded that the use of salicylic acid as a growth regulator can contribute to the enhancement of phytochemical content, especially secondary metabolites such as phenolic and flavonoid compounds, anthocyanins, and the elevation of defensive parameters such as the activation of antioxidant enzymes, as well as an increase in osmolyte content like proline in Roselle plants. These effects assist the plants in exhibiting greater resistance to drought stress and mitigating the negative impacts of this type of stress on the plant.
Improvement and breeding
M. Momeni; A. Ghasemi Pirbalouti; A. Mousavi; H.A. Naghdibadi
Abstract
Background and objectives: Thymbra spicata L. belongs to the Lamiaceae family and is a rich source of aromatic compounds and essential oils. This research was conducted to study the effect of salicylic acid and chitosan elicitors on the growth indicators and DXR gene expression in T. spicata L. under ...
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Background and objectives: Thymbra spicata L. belongs to the Lamiaceae family and is a rich source of aromatic compounds and essential oils. This research was conducted to study the effect of salicylic acid and chitosan elicitors on the growth indicators and DXR gene expression in T. spicata L. under different levels of irrigation towards enhancing the performance of this medicinal plant in experimental pots in a greenhouse in Ilam province during 2017-18.Methodology: A factorial design was used with 15 treatments and three replications in a completely randomized study. Irrigation regimes at three levels of normal (A₁), 70% of field capacity (A₂), 40% of field capacity (A₃), and foliar spraying at five levels, including control (B₁), chitosan at concentrations of 0.5 g.l-1 (B₂) and 1 g.l-1 (B₃), salicylic acid foliar spraying at concentrations of 2.5 mM (B₄) and 5.0 mM (B₅) were used at the 10-12 leaf stage. To prepare chitosan and salicylic acid elicitors, the powders were dissolved in 5% acetic acid and then adjusted to desired concentrations with ddH2O. Irrigation regimes were applied three months after cultivation (15-20 cm seedlings). To apply the elicitors simultaneously with the water stress treatment, foliar spraying was done three times at ten-day intervals. Quantitative RT-PCR was used to investigate the expression changes of the DXR gene, which is one of the key genes in the carvacrol biosynthesis pathway. In this regard, total RNA was extracted, and cDNA was synthesized after assessment of its quantity and quality. With the aid of specific primers, the target sequence was amplified. Furthermore, using the GAPDH reference gene reported in Hyssop thyme, the gene expression level was investigated through the relative quantification method. The method of difference in cycle threshold (2-∆∆CT) and Relative Expression Software Tool (REST) were used to analyze the data.Results: The analysis of the variance table showed the effect of different levels of irrigation on all quantitative growth indices, including plant height, root length, number of main branches, root fresh and dry weight, leaf fresh weight, stem fresh and dry weight, except leaf dry weight, were significant (p<0.01). The interactive effect of low irrigation and foliar spray of salicylic acid and chitosan on root fresh weight, leaf fresh weight, and stem fresh weight was significant at 1%. Accordingly, the interaction effect of irrigation and foliar spraying on the dry weight of the stem was significant at the 5% probability level. The results showed that the interactive effect of irrigation and foliar spray on leaf dry weight, number of main branches, and root length was insignificant. In this study, DXR gene expression did not show many changes after applying two different levels of chitosan (0.5 and 1.0 g.l-1). In contrast, salicylic acid treatment with 2.5 mM was significant at 1% probability and caused an increase in gene expression. The transcript level of this gene increased 29.72 times after applying 5.0 mM of this solution, which was significant at the 1% level. The amount of low irrigation decreased this gene's expression.Conclusion: Based on the obtained data, the normal irrigation method is the most suitable for hyssop thyme plants regarding their growth indicators. Foliar spraying of chitosan at different levels did not significantly affect growth indices and DXR gene expression. At the same time, salicylic acid increased them in this species. As a result, salicylic acid elicitor can increase carvacrol yield and biosynthesis.
Improvement and breeding
M. Edalateyan Kharazi; A. Asgharzadeh
Abstract
To investigate the effects of drought stress, salicylic acid, and polyamines on plant growth yield and oil and seeds essential oil content in Nigella sativa L., a factorial experiment was conducted in a completely randomized design with three replications in the research greenhouse of Shirvan Azad University ...
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To investigate the effects of drought stress, salicylic acid, and polyamines on plant growth yield and oil and seeds essential oil content in Nigella sativa L., a factorial experiment was conducted in a completely randomized design with three replications in the research greenhouse of Shirvan Azad University in 2020-21 cropping year. The experimental treatments included drought stress at four levels (25, 50, 75, and 100% of field capacity) as the first factor and foliar application of salicylic acid, putrescine, and spermidine each at two levels (0.5 and 1 mM) as the second factor. The results showed that the highest number of fertile capsules per plant (12.66), number of seeds per capsule (96.24), and harvest index (58.69%) were obtained in the 100% of field capacity treatment. Also, the highest seed yield per pot was obtained in the 100% of field capacity treatment (16.05 g) and 1 mM salicylic acid (11.46 g). On the other hand, the highest plant height (62.5 cm), dry weight of single plant (6.44 g), biomass in pot (32.2 g), oil content (31.21%), and oil yield (5.71 g.pot-1) were obtained in the treatment of 100% of field capacity + 1 mM salicylic acid and also, the highest essential oil content (0.09%) was obtained in the treatment of 25% of field capacity + 1 mM salicylic acid. Overall, the results showed that full irrigation (100% of field capacity) + 1 mM salicylic acid foliar application was the best treatment for N. sativa under greenhouse conditions. In general, the plant growth characteristics decreased with increasing stress severity, but the negative effects of drought stress were moderated with increasing the salicylic acid foliar application.
Agriculture and horticulture
B. Andalibi; M. Mohammadi Azar; B. Esmailpour; F. Shekari
Abstract
This study was conducted to investigate the effects of salicylic acid (SA) and nanosilicon (n-Si) on some morphophysiological characteristics and essential oil of Lallemantia iberica under salinity stress and uncontrolled greenhouse conditions as a factorial experiment based on the randomized complete ...
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This study was conducted to investigate the effects of salicylic acid (SA) and nanosilicon (n-Si) on some morphophysiological characteristics and essential oil of Lallemantia iberica under salinity stress and uncontrolled greenhouse conditions as a factorial experiment based on the randomized complete block design in 2018. The experimental factors included sodium chloride (0, 50, and 100 mM), SA (0, 1, and 2 mM), and n-Si (0, 0.5, and 5 mM). The SA and n-Si treatments were applied at the four-leaf stage (once every seven days) and the salinity treatment was applied at the six-leaf stage of the plant (once every four days) until fully ripening seeds of the plant (yellowing 90% of the leaves and capsules). The results showed that under salinity stress, the plant traits including height, aerial parts dry weight, chlorophyll content, and seed yield decreased and proline and essential oil increased. The application of SA and n-Si improved these traits under salinity stress. The main constituents of L. iberica essential oil included linalool (25%), geraniol (16%), β-cubebene (10.6%), menthyl acetate (9.8%), valencene (3.5%), germacrene-D (2.4%), β-caryophyllene (2.3%), and limonene (1.3%), all of which increased in the salinity treatments compared to the control. The application of SA and n-Si under salinity stress conditions increased limonene, linalool, geraniol, β-cubebene, β-caryophyllene, and valencene and decreased menthyl acetate and germacrene D. In general, the results of this study showed that the application of SA and n-Si could increase the tolerance of L. iberica to salinity stress by creating the osmotic regulation, protecting the content of photosynthetic pigments, and reducing oxidant damage.
M. Ababaf; H. Omidi; A.M. Bakhshandeh
Abstract
This research was aimed to investigate the foliar application of Plant Growth Regulators (PGRs) on growth parameters, yield, some morphophysiological traits, and alkaloids of rosea periwinkle (Catharanthus roseus L. (G. Don) under drought stress conditions. The field study was conducted based on split-plot ...
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This research was aimed to investigate the foliar application of Plant Growth Regulators (PGRs) on growth parameters, yield, some morphophysiological traits, and alkaloids of rosea periwinkle (Catharanthus roseus L. (G. Don) under drought stress conditions. The field study was conducted based on split-plot factorial in the randomized complete block design (RCBD) with three replications at the research farms of Shahid Chamran University in Ahwaz city in 2016-17. The experimental treatments included drought stress as the main factor at three levels of 40% (control), 60% and 80% of maximum allowable depletion (MAD) and foliar application of jasmonic acid (JA) at two levels (0 and 10 µM) and salicylic acid (SA) at three levels (0, 0.1, 1, and 10 mM) as subplots. Based on the results of the mean comparison of main effects, drought stress (80% MAD) reduced 36.6% of fresh weight and 25.4% of the dry weight of the plant compared to the control. The treatment 10 mM SA resulted in 13.4, 125.5, and 59.6% increases in plant height, leaf area and flower dry weight, respectively, compared to the control. The foliar application of JA increased flower dry weight and plant fresh weight by 16.3 and 8.6%, respectively. The results showed that the foliar application of SA in concentrations of 1 and 10 mM caused the highest content of vincristine and vinblastine, respectively. The interaction of both PGRs and drought stress showed the highest alkaloids content. The highest vinblastine and vincristine under severe drought stress were obtained in foliar application of 1 mM SA+10µM JA and 10 mM SA+10µM JA, respectively.
Z. Taghizadeh Tabari; H.R. Asghri; H. Abbasdokht; E. Babakhanzadeh sajirani
Abstract
Water deficit has been the major contributor to the decline in plant yield. Soil amendment and certain hormonal agents are some strategies applied to mitigate the adverse effects of drought stress. The purpose of this study was to investigate the effects of one soil modifier and one growth regulator ...
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Water deficit has been the major contributor to the decline in plant yield. Soil amendment and certain hormonal agents are some strategies applied to mitigate the adverse effects of drought stress. The purpose of this study was to investigate the effects of one soil modifier and one growth regulator on some physiological and morphological characteristics of Borago officinalis L. under water deficit conditions. The experiment was implemented as a split-factorial in a randomized complete block design with four replications at the research farm of Agriculture Faculty of Shahroud University of Technology, Iran during 2017- 2018. The main plots consisted of three levels of irrigation (usual irrigation every five days, irrigation every 10 and 15 days) and sub-plots consisted of biochar at three levels (0, 5 and 10 t ha-1) and salicylic acid at two levels (0 and 0.5 mM). Based on the results, biochar, salicylic acid, and water deficit factors affected the number of flowering stems, number of stems, stem height, the amount of chlorophylls a and b, total chlorophyll, and carotenoids significantly. Interactions between salicylic acid and water deficit levels had the most effects on carotenoids and chlorophyll b. Triple interaction of factors also affected the total dry weight, number of flowering stems, the number of stems, stem height, and chlorophyll b. Generally, water deficit stress decreased the amount of photosynthetic pigments and leaf growth indices of European borage compared to control, and salicylic acid and biochar reduced the negative effects on the amount of photosynthetic pigments. Therefore, the use of biochar as a soil modifier and salicylic acid as a growth regulator seems to be useful in ameliorating some of the negative effects of water stress on Borago officinalis.
H. Darvizheh; M. Zahedi; B. Abaszadeh; J. Razmjoo
Abstract
In order to study the effect of water deficit and foliar application of salicylic acid and spermine on the phenological stages and caffeic acid derivatives yield of Echinacea purpurea L., a three replicated split- plot experiment was conducted based on a randomized complete block design during 2016 and ...
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In order to study the effect of water deficit and foliar application of salicylic acid and spermine on the phenological stages and caffeic acid derivatives yield of Echinacea purpurea L., a three replicated split- plot experiment was conducted based on a randomized complete block design during 2016 and 2017 growing seasons at the research field of Research Institute of Forests and Rangelands, Iran. In this experiment, three irrigation regimes (irrigation after 20, 40 and 60% depletion of soil available water) were accommodated in main plots and salicylic acid (SA) and spermine (SPM) spray treatments (Control (no spray), spray at five levels of 75 mg/l SA, 150 mg/l SA, 75 mg/l SPM, 75 mg/l SA+75 mg/l SPM and 150 mg/l SA+75 mg/l SPM) were considered in subplots. The exogenous SA and SPM were applied on the foliage at two steps, 10-days apart and at the beginning of stem elongation stage. The effects of irrigation regime and spray treatments were significant on the traits studied. Drought stress decreased the number of days to phenological stages, the yield of echinacoside and cynarin in flower and cichoric acid, and caftaric acid and cynarin in leaves, but increased the yield of chlorogenic acid in flowers and caffeic acid derivatives (cichoric acid, echinacoside, chlorogenic acid, caftaric acid and cynarin) in roots. The foliar application of salicylic acid and spermine increased all studied traits. The interaction between water deficit and foliar spray was significant on the yield of caffeic acid derivatives in roots. The highest yield of cichoric acid (9.49 kg/ha), echinacoside (0.67 kg/ha), chlorogenic acid (0.26 kg/ha), caftaric acid (7.31 kg/ha) and cynarin (0.27 kg/ha) in roots was obtained at irrigation after 60% depletion of soil available water and simultaneous application of high concentrations of SA and SPM. Based on the results of the present study, the foliar application of SA and SPM improved the yield of caffeic acid derivatives in coneflower under both normal and water stress conditions.
F. Heidari; F. Shekari; B. Andalibi; J. Saba
Abstract
In order to investigate the effects of growth regulators on yield and agronomical characteristics of saffron (Crocus sativus L.), an experiment was conducted in a randomized complete block design in the research greenhouse of Faculty of Agriculture, University of Zanjan. Experimental treatments included ...
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In order to investigate the effects of growth regulators on yield and agronomical characteristics of saffron (Crocus sativus L.), an experiment was conducted in a randomized complete block design in the research greenhouse of Faculty of Agriculture, University of Zanjan. Experimental treatments included corms priming with gibberellic acid (GA) (250, 500 and 750μM), salicylic acid (SA) (700, 1400 and 2100μM), paclobutrazol (PBZ) (50, 100 and 150μM), chlormequat chloride (CCC) (500, 1000 and 1500 μM), hydropriming (HP) (distilled water), and control (treatment without any growth regulator). Among the treatments, the effect of GA was more pronounced than other growth regulators, so that the highest dry yield of flower and stigma was observed in GA treatment, especially at 500-μM level. CCC and PBZ caused the lowest flower and stigma yield and the highest amount of chlorophyll pigments compared to other treatments. In contrast, the use of GA and SA reduced the chlorophyll content. HP treatment did not show any significant difference with control. SA treatment increased safranal (perfume) and crocin (color) of the stigmas. The highest effect on picrocrocin (flavor) was obtained in CCC treatment.
H. Molahoseini; M. Feizian; S. Davazdaemami; E. Mehdi Pour
Abstract
In order to evaluate the effects of silicone nano oxide coated with humic acid and salicylic acid on some morphological parameters and ionic composition of black cumin (Nigella sativa L.) under salinity stress, two set of experiments were conducted (in lab. and greenhouse) during 2016-2017. First, silicone ...
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In order to evaluate the effects of silicone nano oxide coated with humic acid and salicylic acid on some morphological parameters and ionic composition of black cumin (Nigella sativa L.) under salinity stress, two set of experiments were conducted (in lab. and greenhouse) during 2016-2017. First, silicone nano oxide was coated by different concentrations of humic acid (40-1600 mg l-1) in the presence of salicylic acid (pH=4) and at different times (0.5 to 120 hours) to maximize the coverage and energy absorption. Then, the effects of coated silicone nano oxide were evaluated in salinity stress in a split plot experiment based on randomized complete blocks with four replications. The salinity treatment was applied in three levels including 2 ds m-1 as control, 3.5 and 5 ds m-1 with irrigation. The coated silicone nano oxide treatments included 0, 0.25, 0.5 and 0.75 g l-1, applied via irrigation at two growth stages of black cumin (vegetative and reproductive). The results of laboratory experiment showed that by increasing humic acid concentration and exposure time (in presence of salicylic acid), the carbon adsorption rate increased from 6.6 to 11.9 mg g-1. However, the size of silicone nano oxide was not significantly different (20 to 30 nanometers). Results of analysis of variance showed that changes in dry weight of shoot, root, plant height, shoot/root ratio, number of branches, relative water content, and potassium in the salinity treatments and coated nanoparticles were independently significant. It is concluded that the silicone nano oxide coated with humic acid and salicylic acid can result in increased salinity tolerance of the black cumin through reducing the amount and frequency of these materials.
M. Parsa; A. Zeinali
Abstract
Tropane alkaloids such as atropine and scopolamine have wide application in the treatment of diseases such as asthma and antispasmodic due to anticholinergic agents. In the present study, the effects of salicylic acid (SA) on the production of two alkaloids, atropine and scopolamine ,were studied ...
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Tropane alkaloids such as atropine and scopolamine have wide application in the treatment of diseases such as asthma and antispasmodic due to anticholinergic agents. In the present study, the effects of salicylic acid (SA) on the production of two alkaloids, atropine and scopolamine ,were studied in hairy root and in vitro grown root cultures of Hyoscyamus niger L. The roots were cultured in liquid B5 medium containing different concentrations of SA (0, 0. 1, 1, 2 and 4 mM) in various exposure times (24, 96 and 168 hours). Eventually, root growth index, and atropine and scopolamine content were assayed after 30 days. In in vitro grown roots, treatment with 1mM SA resulted in the highest production of atropine after 168 hours, while the highest amount of scopolamine (649.53 µg/g D. W) was obtained in 2mM SA (after 96 hours), showing more than 13-fold increase compared to the control. In hairy root cultures, the most significant contents of atropine were observed in the medium containing 2mM SA after 96 h. Moreover, the highest content of scopolamine was achieved in medium treated with 0.1 mM SA after 96 hours. In general, atropine content in hairy roots was considerably higher than that of in vitro grown roots. In contrast, scopolamine content in in vitro grown roots was significantly more than that of hairy roots. Moreover, the rate of root growth declined as a result of increasing of elicitor concentration in the medium.
T. Izan; F. Shekari; Y. Nasiri
Abstract
Moldavian balm (Dracocephalum moldavica L.), belonging to the Lamiaceae family, is an annual and aromatic plant, which is used in traditional and modern medicine in the treatment of gastrointestinal and cardiovascular disorders. In order to evaluate the effect of salicylic acid on yield and essential ...
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Moldavian balm (Dracocephalum moldavica L.), belonging to the Lamiaceae family, is an annual and aromatic plant, which is used in traditional and modern medicine in the treatment of gastrointestinal and cardiovascular disorders. In order to evaluate the effect of salicylic acid on yield and essential oil of Moldavian balm under drought stress conditions, a field experiment was conducted as a split plot based on randomized complete blocks design with three replications at the Department of Natural Resources and Watershed of Piranshahr City in 2012. The main factor was irrigation at three levels (40, 80 and 120 mm evaporation from pan), and the sub factor was plant spraying with salicylic acid at three levels (0, 0.5 and 1 mM). Results showed that the effect of drought stress on dry yield, thousand seed weight, harvest index of flowering stem, and essential oil yield was significant. Similar results were obtained for the effect of plant spraying with salicylic acid on dry weight and essential oil yield. It should be noted that dry yield and essential oils yield were most affected by drought stress. In addition, the highest values of thousand seed weight and harvest index of flowering stem was related to the irrigation treatment of 120 mm evaporation. The present study results also showed that the interaction effect of two factors studied on essential oil percentage and essential oil harvest index was significant. In this regard, the maximum amount of essential oil and essential oil harvest index belonged to application of 1 mM salicylic acid in irrigation treatment of 120 mm evaporation from pan.
F. Mohtashami; M. Pouryousef; B. Andalibi; F. Shekari
Abstract
To study the effects of seed priming and foliar application of salicylic acid (SA) on yield and essence of fennel (Foeniculum vulgar Mill.) under drought stress condition, a field experiment was conducted at College of Agriculture, University of Zanjan in the spring of 2012. The experiment was conducted ...
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To study the effects of seed priming and foliar application of salicylic acid (SA) on yield and essence of fennel (Foeniculum vulgar Mill.) under drought stress condition, a field experiment was conducted at College of Agriculture, University of Zanjan in the spring of 2012. The experiment was conducted as a split plot based on complete randomized block design with three replications. In this study, drought stress at reproductive stage with control (full irrigation) was assigned to the main plots and different treatments of seed priming and foliar application with SA including dry seeds (without priming), seeds priming with SA at different concentrations (0, 800 and 1600μmol) and foliar application with SA at different concentrations (800 and 1600μmol) were assigned to the subplots. Results showed that the majority of measured traits including number of umbrella in plant, number of seeds in umbrella, seed thousand weight, harvest index, biomass, grain yield, and essential oil yield significantly decreased under drought stress condition and the essential oil content increased vice versa. Therefore, the highest seed (2925.6kg/ha) and essential oil yield (52.88kg/ha) were obtained in control, and the lowest amount of mentioned traits was obtained in drought stress condition. The results also showed that the effect of seed priming and foliar application of SA on the majority of measured traits was significant. There were no differences about seed yield and essential oil yield between foliar application and priming with SA at a concentration of 1600 μmol; however, the highest seed yield (2579.60kg/ha) and essential oil yield (63.15kg/ha) were obtained in foliar application with SA at a concentration of 1600μmol, and the lowest amounts of mentioned traits were obtained in dry seeds (without priming).
A. Faraji; B. Esmailpoor; F. Sefidkon; B. Abaszadeh; K. Khavazy
Abstract
In order to investigate the effect of salicylic acid and putrescine as foliar spray on plant yield and growth, essential oil yield and composition of summer Savory (Satureja hortensis L.), a field experiment was carried out based on randomized complete blocks design (RCBD), with three replication. ...
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In order to investigate the effect of salicylic acid and putrescine as foliar spray on plant yield and growth, essential oil yield and composition of summer Savory (Satureja hortensis L.), a field experiment was carried out based on randomized complete blocks design (RCBD), with three replication. Different concentrations of salicylic acid (0, 1, 2 and 3mM) and putrescine (0, 0.5, 1, 1.5 and 2mM) were applied as foliar spray at early stages of shooting, blooming and floweing. Results showed that foliar application of salicylic acid and putrescine had significant effect (p<0.01) on some vegetative parameters such as dry weight and yield of total aerial shoots, leaves and inflorescences as well as total chlorophyll concentration. All treatments had a significant effect (p<0.01) onessential oil efficiency, percentage, yield and composition of Satureja hortensis. The highest value of total aerial shoots dry weight (45.63g/p), total aerial shoots yield (5070kg/ha), leaf dry weight (6.39g/p), leaf yield (710kg/ha), inflorescence dry weight (15/65g/p), inflorescence yield (1739kg/ha), and total chlorophyll concentration (1.46mg/l) were observed in 3mM salicylic acid. The essential oil efficiency (2.35kg/ha) and essential oil yield (76.76%) reached a maximum range in 1.5mm putrescine. The concentration of thymol (47.76%) as the main volatile component of summer savory, reached the highest value in 2mM salicylic acid while other components, such as α-pinene, limonene and E-B-ocimene, reached the highest value in 50mg/L putrescine.
P. Haiati; V. Rowshan
Abstract
Satureja hortensis L. (Savory), belonging to the family Lamiaceae (formerly Labiatae) is an annual herb. In order to determine the effects of salicylic acid (SA) (Control, 200, 4oo and 600mgL-1) in early flowering stage on growth and components of essential oils of Satureja hortensis, a factorial ...
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Satureja hortensis L. (Savory), belonging to the family Lamiaceae (formerly Labiatae) is an annual herb. In order to determine the effects of salicylic acid (SA) (Control, 200, 4oo and 600mgL-1) in early flowering stage on growth and components of essential oils of Satureja hortensis, a factorial experiment based on randomized complete block design was carried out with three replications. The oil was analyzed by GC and GC/MS. The fresh weight was significantly increased in 200 mgL-1 SA, compared to the control. The yield of essential oil was 3.1% (Control) and 3.7% (200 mgL-1) with a significant difference. The main constituents of the essential oil were carvacrol, γ-terpinene, α-terpinene and p-cymene. SA application increased α-terpinene from 4.35% (Control) to 7.7% (200 mgL-1), β-myrcene from 2.5 (Control) to 4.3 (200 mgL-1), p-cymene from 1.9% (Control) to 2.5% (600 mgL-1) and decreased carvacrol from 44.8% (Control) to 38.5% (200 mgL-1). Results showed that SA application caused changes in the quantity and quality of essential oils.
M. Zarooshan; F. Bernard; Z. Heydarian
Abstract
Thymus daenensis Celak. is an endemic plant species in Iran used for medicinal purposes. It seems that In vitro culture is a useful technique for its propagation. In vitro culture conditions cause water stress or hyperhydricity in tissues due to the cut in the plant tissue and changes in levels of plant ...
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Thymus daenensis Celak. is an endemic plant species in Iran used for medicinal purposes. It seems that In vitro culture is a useful technique for its propagation. In vitro culture conditions cause water stress or hyperhydricity in tissues due to the cut in the plant tissue and changes in levels of plant hormones compared to natural conditions and may vary depending on the type of medium, type of agar and available water. Hyperhydricity syndrome is one of the major problems that occur in in vitro growth and through a deformity prevents the propagation of plants. In shoot culture of Thymus daenensis Celak., hyperhydricity happens commonly. In this investigation, the effect of benzyladenine and salicylic acid were studied on shoot hyperhydricity syndrome. For this purpose, disinfected seeds were cultured into glass jars containing Murashige and Skoog (MS) medium with benzyladenine (0.1mgl-1). Non benzyladenine-treated plantlets were transferred to the same medium and benzyladenine-treated plantlets were cultured in four experimental media including without benzyladenine, with 1mgl-1 benzyladenine in presence or without 5µM salicylic acid. Afterward, effects of the mentioned treatments on hyperhydricity, morphological and physiological characteristics of the plant were measured. Results showed that benzyladenine singly caused hyperhydricity in shoot explants of Thymus daenensis. After transferring the explants to the medium free of benzyladenine, hyperhydricity reversed toward normal state and the presence of salicylic acid improved this reversion. Salicylic acid reduced water content in shoot explants and enhanced the amount of photosynthetic pigments. In benzyladenine treated sample, the hyperhydricity was not reversed by salicylic acid.
Sh. Hashemi; Z. Asrar; Sh. Pourseyedi
Abstract
Lepidium sativum L. is known as a medicinal plant. This plant is regarded as a cure for breast cancer due to antioxidant properties. The availability of manganese (Mn) in plants is governed by redox processes which depend upon pH and the presence of electrons. Excess Mn disturbs the ...
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Lepidium sativum L. is known as a medicinal plant. This plant is regarded as a cure for breast cancer due to antioxidant properties. The availability of manganese (Mn) in plants is governed by redox processes which depend upon pH and the presence of electrons. Excess Mn disturbs the metabolism of plants and inhibits the plant growth and oxidative stress is also induced which leads to the formation of reactive oxygen species (ROS) including superoxide, hydroxyl and hydrogen peroxide that could damage plant cells. Salicylic acid (SA) is a natural signal molecule which plays an important role in regulating a number of physiological processes and plant resistance to biotic and abiotic stresses. The protective function of SA includes the regulation of ROS, antioxidants, induction of gene expression, absorption and distribution of elements. In this investigation, the effects of different levels of salicylic acid (0, 0.01, 0.05, 0.1mM) on different concentrations of Mn (0, 250, 500, 800µM) were studied on Lepidium sativum. Results showed that addition of SA (0.01, 0.05, 0.1mM) improved the inhibition of induced calcium and magnesium uptake. Ascorbare content in leaf was increased by SA (0.01, 0.05, 0.1mM) but decreased by concentrations of 500 and 800 µM Mn. The proline content significantly increased in treatments of Mn and SA. The protein content decreased by 500 and 800 µM but the treatment of SA (0.01, 0.05, 0.1mM) increased the protein content under conditions of manganese toxicity.
R. Khavari-nejad; A. Asadi
Abstract
Plants produce a diverse array of secondary metabolites. These compounds may be synthesized during normal growth and development and accumulating only in response to pathogen attack or stress. Interest in these molecules stems from their medicinal properties, antimicrobial activity and their likely role ...
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Plants produce a diverse array of secondary metabolites. These compounds may be synthesized during normal growth and development and accumulating only in response to pathogen attack or stress. Interest in these molecules stems from their medicinal properties, antimicrobial activity and their likely role as determinats of plant disease resistance. Daisy (Bellis perennis L.) accumulates secondary compounds (triterpenoid saponins and anthocyanins) in response to SA and pathogens. The results of the research indicates that the amount of secondary compounds (saponins and anthocyanins) in treated plants with SA were more than in the control plants. The amount of compounds in infected plants and without SA was more than in the control plants. When infected plants were treated with SA, an increase of compounds was shown in plants. The peaks observed in HPLC and their comparision with compounds standard confirms the results mentioned above. It is concluded that SA have significantly reduced disease severity and increase the amount of secondary compounds in infected and non-infected Daisy plants.
