J. Hasani; M. Mirza
Abstract
This study was conducted to evaluate the essential oil (EO) quality and quantity of six thyme species (Thymus spp.), growing in the natural habitats of Kurdistan province, in 2010. To conduct this research, six species of thyme were identified in different habitats and used for essential oil extraction. ...
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This study was conducted to evaluate the essential oil (EO) quality and quantity of six thyme species (Thymus spp.), growing in the natural habitats of Kurdistan province, in 2010. To conduct this research, six species of thyme were identified in different habitats and used for essential oil extraction. Water distillation (Clevenger-type apparatus) was used to obtain the EOs and the components were identified using the gas chromatography (GC) system and a gas chromatograph connected to mass spectrometer (GC/MS). The results showed that more than 30 components were identified in the EOs, among which thymol, carvacrol, geraniol, alpha terpinole and linalool were the most important components. The EO yields were 3.05%, 2.53%, 2.83%, 1.51%, 1.31% and 1.05% for T. fallax, T. daenensis, T. pubescens, T. transcaucasicus, T. fedtschenkoi and T. kotschyanus, respectively. The content of thymol was calculated to be 70.6%, 62.2%, 27.8% and 1.5 in the EOs of T. daenensis, T. fedtschenkoi, T. pubescens and T. kotschyanus, respectivelyand the content of carvacrol was recorded to be 3.43%, 4.80% and 0.74% in the EOs of T. daenensis, T. fedtschenkoi and T. pubescens, respectively.
Sh. Abbasi; S. Houshmand; N. Mirakhorli; R. Ravash
Abstract
The leaves of thyme are the most important organs producing essential oils, and the glandular trichomes provide a unique capacity for the synthesis and storage of active ingredients. In this study, the morphological characteristics and types of trichomes' structure were measured in four thyme species ...
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The leaves of thyme are the most important organs producing essential oils, and the glandular trichomes provide a unique capacity for the synthesis and storage of active ingredients. In this study, the morphological characteristics and types of trichomes' structure were measured in four thyme species including: T. transcaucasicus, T. carmanicus, T. daenensis and T. armeniacus. The results of variance analysis indicated a significant difference between species in terms of stem length, leaf length and number, plant fresh and dry weight, leaf and stem dry weight, number of glandular trichomes on the adaxial and abaxial side of the leaf, the trichome length, and essential oil yield and percentage. T. armeniacus showed the highest trichome number; while, the highest trichome length was related to T. transcaucasicus. The highest essential oil percentage and yield were observed in T. carmanicus and T. daenensis respectively, which could be due to the greater number of glandular trichomes and leaves in these species. The results of scanning electron microscope (SEM) revealed that among various types of glandular trichomes, peltate glandular trichome existed in the leaf of four species examined. Positive and significant correlation coefficients between the number of glandular trichomes on the adaxial and abaxial side of the leaves with the essential oil percentage and yield indicate the role of this feature in the production of essential oil. The stepwise regression analysis, implemented for dry weight, indicated that the highest regression positive coefficient was observed for the leaf dry weight, explaining 97% of the trait variation. When the essential oil yield was assumed as a dependent variable, only the trichome length entered the model and explained 49% of the trait variation.
M.H. Lebaschi; E. Sharifi Ashoorabadi; M. Makizadeh Tafti; S. Asadi-Sanam; Kh. Karimzadeh Asl
Abstract
In order to evaluate the effect of plant density on quantitative and qualitative yieldof three species of Thymus under dry farming conditions in Tehran (Damavand), Kurdistan and Kermanshah provinces, an experiment was conducted in a randomized block design based on split plot with three replications ...
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In order to evaluate the effect of plant density on quantitative and qualitative yieldof three species of Thymus under dry farming conditions in Tehran (Damavand), Kurdistan and Kermanshah provinces, an experiment was conducted in a randomized block design based on split plot with three replications during three years (2009-2011). The treatments were included three species (T. vulgaris L., T. daenensis Celak.and T. kotschyanus Boiss. & Hohen.) and three planting densities (4, 6 and 8 plant/m2), which were considered as main and sup-plots, respectively. Shoot dry matter yield, essential oil content, yield and essential oil components were determined. Results showed that the four-interaction effect (density, species, year and province) was significant on dry matter content, calculated to be from 75 to 5172 kg/h in different treatments. The maximum dry matter yield was observed at a density of 6 plant/m2 for T. daenensis in Kermanshah in the third year of experiment. The highest essential oil yield was obtained at a density of 8 plant/m2 for T. daenensis in Kermanshah in the second year of experiment. The maximum thymol content (80.33%) was related to T. daenensis inDamavand at a density of 6 plant/m2 in the third year of experiment. Overall, high production capacity of T. daenensis in the study provinces indicates the adaptation and economic yield of this native and valuable species.
M. Askary; M.A. Behdani; S. Parsa; M. Jamialahmadi; S. Mahmoodi
Abstract
This research was aimed to evaluate the effects of drought and manure on some physiological characteristics and yield of Thymus vulgaris L. and Thymus daenensis Celak.The study was conducted as factorial based on a randomized complete block design (RCBD) with three replications during crop years of 2015 ...
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This research was aimed to evaluate the effects of drought and manure on some physiological characteristics and yield of Thymus vulgaris L. and Thymus daenensis Celak.The study was conducted as factorial based on a randomized complete block design (RCBD) with three replications during crop years of 2015 and 2016 at the agriculture research farm of University of Birjand, Iran. Experimental factors were including: water stress at three levels (100%, 67% and 33% of field capacity), manure at two levels (30 tons.ha-1 and no manure), and two thyme species (Thymus vulgaris L. and Thymus daenensis Celak). Water stress reduced stomatal conductance, relative water content (RWC), Chl a, b and quantitative and qualitative yield; however, carotenoids content was increased under water stress. Although the quantity and quality yield of the study species decreased under water stress condition, manure application at 30 tons per ha led to the increased quantity and quality yield of both species. Thymus daenensis had more relative water content and quantity and quality yield (oil) in both crop years as compared with T. vulgaris. According to the results, T. daenensis can grow successfully in arid and semi-arid regions and could be recommended. There was a significant difference between two crop years; in the 2nd year, the quantity and quality yield (essential oil yield) of both study species increased.
M. Nasiri; S.E. Seedian; E. Sharifi Ashorabadi
Abstract
Thymus, belonging to Lamiaceae family, is one of the most important medicinal genera in Iran. It has many uses in food, health care, cosmetics and especially in pharmaceutical industries. This research was aimed to investigate the germination parameters and establishment of seedlings from different accessions ...
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Thymus, belonging to Lamiaceae family, is one of the most important medicinal genera in Iran. It has many uses in food, health care, cosmetics and especially in pharmaceutical industries. This research was aimed to investigate the germination parameters and establishment of seedlings from different accessions of Thymus species(T. daenensis, T. fedchenkoi, T. kotschyanus, T. lancifolius, T. migricus, T. pubescens, T. transcaspicus and T. vulgaris). All the accessions are stored in Natural Resources Gene Bank of Iran. The seeds of accession were son in pots using a completely randomized design with three replications. Data were collected for thousand seed weight, seed germination percentage, seed storage time, germination rate, vegetative growth rate (2, 4, 6 and 8-leaf stage and the emergence of lateral branches), and the establishment percentage of each accession was measured in greenhouse. Unknown accessions were identified after preparing the herbarium specimens. Results indicated positive and significant correlations between 1000-seed weight and germination percentage as well as between seedling establishment and germination percentage (p<0.05); however, no significant correlation was found with emergence of leaves. The highest 1000-seed weight was observed in T. kotschyanus and T. lancifolius, respectively and the highest percentage of germination in T. pubescens. Positive and significant correlations were observed between primary and secondary seed germination percentage and germination rate; however, leaf emergence and lateral branch emergence were negatively correlated. Secondary seed germination showed significant positive correlation with germination rate (p<1% and 5%, respectively). Germination rate and leaf emergence showed positive significant correlation. There were no significant correlations between leaf emergence and lateral branch emergence of different species.
M. Mirza; E. Sharifi Ashoorabadi; B. Allahverdi Mamaghani
Abstract
In order to investigate the quality and quantity of essential oil of Thymus species, an experiment was conducted under field condition at Research Institute of Forests and Rangelands. This project was related to national project, entitled extraction, qualitative and quantitative analyses of the essential ...
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In order to investigate the quality and quantity of essential oil of Thymus species, an experiment was conducted under field condition at Research Institute of Forests and Rangelands. This project was related to national project, entitled extraction, qualitative and quantitative analyses of the essential oil of different Thymus species in some provinces of Iran. For This purpose, seeds of 75 accessions, collected from various provinces, were cultivated under greenhouse condition. Then, seedlings were transferred to the experimental field and cultivated under field condition. Flowering shoots were collected at 50% of flowering stage, dried in shadow and grinded. Essential oil extraction was carried out by hydro distillation method (Clevenger apparatus-Britain pharmacopeia) for three hours. Chemical compounds were identified with GC and GC-MS. According to the results, essential oil yield was between 0.04%-2%. The highest essential oil yield in relation to dry weight belongs to T. kotschyanus (2%), T. daenesis (1.92%) and T. vulgaris (1.69%) from west Azerbaijan province, Lorestan province and Markazi province, respectively. Linalool, geraniol, α-terpineol, Thymol, carvacrol, α-terpinyl acetate and geranyl acetate chemotypes were recognized among accessions. During two years, the highest Thymol (76.6%), carvacrol (82.9%) and geraniol (62.7%) were observed in T. daenesis from Markazi province, T. daenesis from Semnan province and T. lancifolius from Fars province.
A. Zarezadeh; H. Madah Arefi; E. Sharifi Ashoorabadi; A. Mirhosseini; M.R. Arabzadeh
Abstract
The Thymus genus, belonging to Lamiaceae family, consists of over 215 different species in the world. Eighteen species are growing naturally in Iran, among which four species are endemic. Different species of thyme are used in the food, pharmaceutical and cosmetics industries. This research, conducted ...
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The Thymus genus, belonging to Lamiaceae family, consists of over 215 different species in the world. Eighteen species are growing naturally in Iran, among which four species are endemic. Different species of thyme are used in the food, pharmaceutical and cosmetics industries. This research, conducted in Yazd province during 2008-2011, was aimed to investigate the phenology and compatibility of different thyme species. The seeds of 69 accessions of unknown thyme species, provided by the natural resources gene bank of Iran, were planted to study the phenology and establishment rate. According to the obtained results, among 69 accessions studied, eight accessions were compatible with the climate conditions of the study station, 28 accessions were fairly compatible, and 33 accessions were less compatible. The average of establishment rate was calculated to be 75%, 68% and 58% in 2009, 2010 and 2011, respectively. The highest establishment rate (100%) was related to accession BHUXD )T. fedchenkoi×T. pubescens(, selected as superior accession, and the lowest establishment rate (7%) was related to accession XDRNJI (T. lancifolius×T. pubescens).
A. Mohammadian; R. Karamian; M. Mirza; A. Sepahvand
Abstract
Thymus is one of the most important genera of the Lamiaceae family, belonging to the Nepetoideae sub family. In this study, the relationship between altitude from sea level and soil physical and chemical characteristics as important factors affecting the quantitative and qualitative essential oils of ...
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Thymus is one of the most important genera of the Lamiaceae family, belonging to the Nepetoideae sub family. In this study, the relationship between altitude from sea level and soil physical and chemical characteristics as important factors affecting the quantitative and qualitative essential oils of Thymus fallax Fisch.et C.A. Mey.were examined. Four natural habitats of this species (Zagheh, Alashtar, Razan and Rhimeleh) were selected. Aboveground samples were collected in flowering stage and the essential oils were extracted with hydrodistilation method. Essential oils composition was determined using GC & GC/MS. Data analysis was performed by SPSS software, and Duncan's Multiple Range Test was used for mean comparison. According to the obtained results, the highest essential oil was recorded for Rhimeleh (2.8 %w/w) as compared to other habitats. The main components in the essential oils were carvacrol, thymol, gamma- terpinen, p-cymen, champhen and geranial. With increasing altitude, the percentage of carvacrol and thymol increased significantly. Some soil characteristics were correlated with the essential oils, presented in a linear regression model.
A. Zarezadeh; A. Mirhossaini; M. Mirza; M.R. Arabzadeh
Abstract
The essential oil of Thymus spp has valuable components such as thymol and carvacrol. The aerial parts of different thymus accessions, cultivated in Shahedied research station of medicinal plants, were collected at flowering stage and then dried in shadow and hydro-distilled for obtaining their essential ...
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The essential oil of Thymus spp has valuable components such as thymol and carvacrol. The aerial parts of different thymus accessions, cultivated in Shahedied research station of medicinal plants, were collected at flowering stage and then dried in shadow and hydro-distilled for obtaining their essential oils. Analysis and identification of chemical composition of the oil were performed by GC and GC/MS. Among 74 cultivated accessions in 2011, the highest amount of oil yield was related to the accessions CFTNJI (4.17%), ZELP (4%), Q (3.72%), NJICFT (3.71%), XDRMKO (3.54%), respectively and the lowest was related to BHUZSE (0.66%). The highest amount of oil production per hectare was related to the accesstions XDRVGY (87.5kg/ha), NJICFT(45.42kg/ha), ZSECFT(40.86kg/ha), BHUMKO (38.31kg/ha), respectively and the lowest was related to CFTMKO (0.9kg/ha). Para-cymene, 1,8-cineol, gamma terpinene, borneol, thymol, carvacrol, geraniol, limonene, acetate geranil, terpineol, linalool, caryophyllene, respectively with values of 28.41%, 31.66%, 20.44%, 29.35%, 71.1%, 81.45%, 76.99%, 43%, 28%, 38.6%, 55.56%, 81%, 20%, were related to the accessions VGYCFT, NJIBHU, MKOMKO, CFTCFT, ZSEQ, NJICFT, CFTQ, MKOQ, CFTZSE, NJIBHU, ZSEQ, NJICFT, CFTQ, MKOQ, CFTZSE, NJIBHU, ZSELP, VGYMKO. In (2012), the highest amount of oil yield was recorded for the accessions Q (4.07%), CFTVGY (3.7%), MKOVGY (3.63%), ZSEQ (3.45%), ZSELP (%3.43), respectively and the lowest was found in VGYMKO (62.0%). The highest amount of oil production per hectare was related to the accessions BHUVGY (74.9kg/ha), XDRVGY (60.9kg/ha), XDRLP (51.78kg/ha), CFT (49.21kg/ha), Q (46.11kg/ha), respectively and the lowest was found in BHUZSE(2.36kg/ha). Generally, in terms of oil yield , oil production and main composition of essential oil, three accessions including NJICFT (Thymus Transcaspicus) with 3.71% essential oil and 45.42kg per hectare oil production and 81.5 % carvacrol, ZSEQ (T. daenensis) with 3.45% essential oil and 37.15 kg/h oil production and 73% thymol, and XDRVGY (T. vulgaris) with 2.75% essential oil and 78.5kg/h oil production and 45.8% thymol were selected as superior accessions.
R. Taheri; M.H. Lebaschy; A. Zakerin; M. Bakhtiari Ramezani; A. Borjian; M. Makkizadeh Tafti
Abstract
In order to study the effects of plant density on yield and essential oil yield of four species of thymus, an experiment was conducted at Homand Research Station as a split plot in a randomized complete block design with three replications. Main plots were four species including Thymus daenensis, T. ...
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In order to study the effects of plant density on yield and essential oil yield of four species of thymus, an experiment was conducted at Homand Research Station as a split plot in a randomized complete block design with three replications. Main plots were four species including Thymus daenensis, T. pubescens, T. kotschyanus and T.vulgaris and sub plots were three levels of plant density including 25, 35 and 50 cm row spacing. Measured characteristics included height, number of lateral branches, root length, canopy diameter, root dry weight, fresh and dry yield, essential oil percentage and yeild. Results indicated significant differences in terms of measured traits. Among the measured traits, the effect of density was significant on number of lateral branch, canopy dimeter, fresh yield, and root dry weight. According to the results of interaction effect between species and density, the highest dry yield and essential oil content (%) was obtained at 25 cm row spacing in T.kotschyanus and the lowest was recorded at 50 cm row spacing in T. daenensis. Results showed that the highest essential oil yield was obtained at 25 cm row spacing for T. kotschyanus and T. daenensis.
M. larti; S. Ghasempour; E. Sharifi Ashorabadi; B. Alizadeh
Abstract
Thyme is one of the most important species in pharmaceutical, food, health and cosmetic industries. In order to study natural habitats of different species from Thymus genus in west Azerbaijan province, several studies were conducted in habitat conditions during 2001-2003. First, habitat surveys were ...
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Thyme is one of the most important species in pharmaceutical, food, health and cosmetic industries. In order to study natural habitats of different species from Thymus genus in west Azerbaijan province, several studies were conducted in habitat conditions during 2001-2003. First, habitat surveys were conducted in different parts of the province and then plot establishment and data collection were performed in the habitats of T. pubescens and T. kotschyanus, in Hajibeyk and Ghoshchi regions, respectively. In this study, in addition to climate and soil data, vegetation indices such as plant height, canopy cover, number of branches and root length were measured. The results of the study showed that T. kotschyanus is distributed at an altitude of 1450-2500 m and slopes of 20-60% in northern slope and with an average rainfall of 400-600 mm while, T. pubescens is distributed at an altitude of 1900-2600 m, and slopes of 10-45 % (east and north), with an average rainfall of 250-400 mm. A soil texture of silty-clay-loam and silty-loam are needed for the growth of T. kotschyanus and T. pubesen, respectively. Essential oil percentage in 50% flowering stage was recorded to be 1/12% and 1/78% in T. pubescens and T. kotschyanus, respectively.
J. Hasany
Volume 20, Issue 1 , April 2004, , Pages 1-18
Abstract
In this survey two genera of aromatic plants (Thymus&ziziphora) from viewpoint of characterization and ecological demands in kurdistan province. Results showed that there were 6 different species of genus of thymus in Kurdistan. Comprising to kotschyanus, fallax, eriocalyx, pubescens , transcaucasicus ...
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In this survey two genera of aromatic plants (Thymus&ziziphora) from viewpoint of characterization and ecological demands in kurdistan province. Results showed that there were 6 different species of genus of thymus in Kurdistan. Comprising to kotschyanus, fallax, eriocalyx, pubescens , transcaucasicus and daenensis with 2 sub species comprising to daenensis and lancifolia in areas with 1560-2200m altitude and mostly in north and west north directions. Prenial species of ziziphora clinopodioides distributed between 1450-2000m altitude and mostly companion with species of Thymus in different areas of Kurdistan. Mentioned aromatic plants in addition to aromatic and medicin value, cause their extensive canopy inflounce on soil conservation.
F. Sefidkon; F. Askari
Volume 12, Issue 1 , May 2002, , Pages 29-51
Abstract
The genus of Thymus presents 14 species in Iran, some of them are endemic (1). In this project some of the endemic and non-endemic Thymus species, named: T. pubescens Boiss. et Kotschy ex Celak, T. carnosus Boiss, T. kotschyanus Boiss and Hohen, T. persicus (Ronniger ex Rech. F.) Jalas and T. serpyllum ...
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The genus of Thymus presents 14 species in Iran, some of them are endemic (1). In this project some of the endemic and non-endemic Thymus species, named: T. pubescens Boiss. et Kotschy ex Celak, T. carnosus Boiss, T. kotschyanus Boiss and Hohen, T. persicus (Ronniger ex Rech. F.) Jalas and T. serpyllum L. were collected from different regions at before flowering and full flowering stage. The air-dried aerial parts of these species were steam distilled for obtaning their essential oils. The oil yields are as follow respectively: at before flowering (0.66%, 0.28%, 0.26%, 0.55% and 0.57%) and at full flowering stage (0.86%, 2.1%, 0.43%, 1.45% and 0.90%).
Totally the oil yields were lower for theses Thymus species before flowering. The highest oil yields were obtained from T. kotschyanus and T. pubescens.
Analysis and identification of chemical composition of the oils were performed by GC and GC/MS.
Thirty-seven components (representing 93.1%-98.3% of the oils) at before flowering stage and thirty-nine components (representing 88.2%-99.3% of the oils) at full flowering stage were identified.
The main components of the oils were as follow, before and full flowering stage, respectively:
T. carnosus, thymol (27.2% and 36.1%), g-terpinene (19.6% and 19.1%), p-cymene (26.2% and 21.3%), b-caryophyllene (2.5% and 2.8%), carvacrol (2.2% and 2.5%) and borneol (1.6% and 1.6%).
T. kotschyanus carvacrol (40.7% and 41.4%), thymol (26.9% and 19.5%), g-terpinene (7.3% and 10.3%), p-cymene (3.9% and 5.3%), b-caryophyllene (1.8% and 2.5%) and borneol (1.3% and 2.4%)
T. persicus (39.0% and 27.1%), thymol (6.5% and 11.9%), g-terpinene (6.1% and 6.5%), p-cymene (7.5% and 10.2%), b-caryophyllene (2.0% and 3.0%) and borneol (1.6% and 2.9%).
T. pubescens carvacrol (64.8% and 48.8%), thymol (11.9% and 13.9%), g-terpinene (6.1% and trace), p-cymene (2.9% and 12.7%), b-caryophyllene (1.5% and 1.3%) and borneol (0.7% and 3.8%).
T. serpyllum, thymol (18.7% and 18.7%), g-terpinene (21.9% and 22.7%), p-cymene (21.1% and 20.7%), b-caryophyllene (7.1% and 0.1%) and borneol (3.9% and 3.1%)
Geraniol (15.7% and 9.4%), Geranyl acetate (5.3% and 5.3%) and a-terpineol (0 and 9.5%) were found just in T. persicus oil before and full flowering stage, respectively. Germacrene D was also found in T. serpyllum oil (6.0% and 5.1%).
