K. Jaimand; M.B. Rezaee; Z. Behrad; M. Mirza; V. Mozaffarian; R. Azady; M. Naderi; M. Golipur; A. Bahmanzadegan; S. Meshkizadeh; Sh. Karimi
Abstract
Hypericum genus is one of the most important medicinal plants in Iran. Currently in Iran, there are 17 herbaceous, perennial and shrub species of Hypericum of which three species are endemic to Iran. This research was aimed to investigate Hypericine content in nine species of Hypericum. For Hypericine ...
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Hypericum genus is one of the most important medicinal plants in Iran. Currently in Iran, there are 17 herbaceous, perennial and shrub species of Hypericum of which three species are endemic to Iran. This research was aimed to investigate Hypericine content in nine species of Hypericum. For Hypericine content 1 gram of plant was extracted in two steps, chloroform extraction then methanol extraction using a Soxhlet device. Hypericin content was measured by HPLC, using the following condition, mobile phase: (methanol 68%, ethyl acetate 20% and sodium hydrosulphate (0.1 M) 12%) and stationary phase C18, and UV detector: set on 590 nm. Generally, no hypericin was detected in different organs of H. androsaemum L. Hypericine content detected in flowers, leaves and stems were: H. apricum Kar. & Kir. (in flowers 0.061% and leaves 0.005%), H.armenum Jaub. & Spach (flower 0.003%), H.asperulum Jaub. & Spach (in flower 0.025%, leaves 0.004% and stems 0.003%), in H.hirsutum L. (flower 0.007%), in H.linarioides Boss. (flower 0.007%), in H.tetrapterum Fries (flowers 0.008%, leaves 0.014%, and stem 0.001%), and H.vermiculare Boiss. & Hausskn. (flowers 0.005%), in H.perforatum L. (flowers 0.124%, leaf 0.028% , stem 0.003).
K. Jaimand; H. Ahrabi Asli; A. Monfared
Abstract
Flavonoids, are a large class of polyphenols, with more than 4000 combinations. They have a antioxidant role in plant photosynthesis and in human body have such as antioxidant, anti-inflammatory, anticancer and profection of the heart. Quercetin is in flavonol group, is used to fight with viruses and ...
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Flavonoids, are a large class of polyphenols, with more than 4000 combinations. They have a antioxidant role in plant photosynthesis and in human body have such as antioxidant, anti-inflammatory, anticancer and profection of the heart. Quercetin is in flavonol group, is used to fight with viruses and cancer cells. In this study, extraction and measurement of quercetin were carried out in A. millefolium L., A. biebersteinii Afan. and A. Tenuifolia Lam. species. Samples were collected in early June 2010 from experiment farms, in Research Institute of Forests and Rangelands, Tehran, Iran. Various extraction methods were tested using different organs (flowers, leaves and stems) performed. The extraction from stem is reported for the first time. In the first extraction was performed soxhlet apparatus using with chloroform solvent for 72 hours. In the second method the previous sample with chloroform solvent extraction had been performed after separating the solvent, adding methanol extraction surgery was performed again. The third method, depending on the amount of plant matter with solvents methanol and acetic acid (ratio 9:1) by small electric mill and was filtered, in the end a new method with solvents, methanol and acetic acid (ratio 9:1) by electric mill crushed and then soaked for a week and was filtered. Then all samples were concentrated into 30 ml. Totally 36 samples were obtained by the amount quercetin contents in 36 obtained samples were measured combined with High-Performance Liquid Chromatography (HPLC). The highest amount of quercetin (2164 ppm) obtained in flower of A. millefolium. In second method with methanol by soxhlet apparatus were 2064 ppm in leaves, and was 2034 ppm in stem, in fourth method Macerated with methanol related to first method in A. millefolium was lowest 127 ppm amount, in stem of A. biebersteinii was 110 ppm and 23 ppm was btained from A. tenuifolia stem.