In collaboration with Scientific Association of Iranian Medicinal Plants

Document Type : Research Paper

Authors

1 Department of Biotechnology, Jahrom University, Jahrom, Iran

2 Department of Agronomy and Plant Breeding, College of Agriculture and Natural Resources, Karaj, Iran

3 Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran

Abstract

In order to evaluate the effect of titanium dioxide nanoparticles on the production of rosmarinic acid and chlorogenic acid, this nanoparticle with eight different concentrations (0, 5, 10, 20, 30, 50, 100 and 15 ppm) in a completely randomized design with three replications at flowering stage was sprayed on Urmia genotype of Dragonhead (Dracocephalum moldavica L.) planted in the greenhouse, college of agriculture, Tehran university. At full flowering stage, plant shoots were harvested and dried in the shade for a week and then the amount of two major phenolic compounds of extraction were analyzed by high-performance liquid chromatography. Excel and SAS software were used for statistical analysis. Results showed statistically significant differences among different treatments. The production of these two compounds raised in low concentration of nanoparticle treatment up to 30 ppm extract, whereas rosmarinic acid content was decreased in high concentration of nanoparticle treatment. Average content of rosmarinic acid and chlorogenic acid in Urmia genotypes was 123.2 and 54µg/g extract, respectively. Therefore, nanopartcle induced the production rate of phenolic compounds in low concentration.

Keywords

Dastmalchi, K., Dorman, H.G., Kosar, M. and Hiltunen, R., 2007. Chemical composition and in vitro antioxidant evaluation of a water soluble Moldavian balm (Dracocephalum moldavica L.) extract. Food Science and Technology, 40:
239-248.
- Ghanndi, A., Sajjadi, S.E., Abedi, D., Yousefi, J. and Daraei-Ardekami, R., 2004. The invitro activity of seven Iranian plants of the Lamiaceae family against Helicobacter pylori. Nigerian Journal of Natural Products and Medicine, 8: 40-42.
- Karn, B., Masciangioli, T., Zhang, W., Colvin, V. and Alivisatos, P., 2005. Nanotechnology and the Environment; Applications and Implications. American Chemical Society, Washington, 416p.
- Kay, C.D. and Holub, B.J., 2002. The effect of wild blueberry (Vacciniun angustifolium) consumption on postprandial serum antioxidant status in human subjects. British Journal of Nutrition, 88: 389-97.
- Lu, C.M., Zhang, C.Y., Wu, J.Q. and Tao, M.X., 2002. Research of the effect of nanometer on germination and growth enhancement of glycine max and its mechanism. Soybean Science, 21: 168-172.
- Neumann, K.H., Kumar, A. and Imani, J., 2009. Plant Cell and Tissue Culture. A Tool in Biotechnology. Springer-Verlag Berlin Heidelberg, 333p.
- Parejo, I., Viladoma, F., Jaume, B., Rosas-Romero, A., Flerlage, N., Burillo, J.S. and Codina, A., 2002. Comparison between the radical scavenging activity andantioxidant activity of six distilled and nondistilled mediterranean herbs and aromatic plants. Journal of Agricultural and Food Chemistry, 50(23): 6882-6890.
- Reynolds, G.H., 2002. Forward to the Future Nanotechnology And Regulatory Policy. Pacific Research Institute, 20p.
- Salehi, M. and Tamaskani, F., 2008. Pretreatment effect of nanosilver on germination and seedling rowth of wheat under salt stress. Proceeding of 1th Iranian Congress in Seed Sciences and Technology, Gorgan, Iran, 12-13 November: 358.
- Sultan, A., Bahang, H., Aisa, H.A. and Eshbakova, K.A., 2008. Flavonoids from Dracocephalum moldavica. Chemistry of Natural Compounds,
44: 366-367.
- Sungkaworn, T., Triampo, W., Nalakarn, P., Triampo, D., Tang, I.M. and Lenbury, Y., 2007. The effects of TiO2 nanoparticles on tumor cell colonies: fractal dimension and morphological properties. International Journal of Biomedical Science, 2(1): 67-74.
- Triantaphyllou, K., Blekas, G. and Boskou, D., 2001. Antioxidant properties of water extracts obtained from herbs of the spices Lamiaceae. International Journal of Food Sciences and Nutrition, 52(4): 313-317.
- Shan, B., Cai, Y.Z., Sun, M. and Corke, H., 2005. Antioxidant capacity of 26 spice extracts and characterisation of their phenolic constituents. Journal of Agricultural and Food Chemistry, 53(20): 7749-7759.
- Zhaobo, W., Sondi, I. and Salopek-Sondi, B., 2005. Study on novel antibacterial high-impact polystyrene/TiO2 nanocomposites. Journal of Materials Science, 40(2): 6433-6438.