Volume 32, Issue 6 , February 2017
A. Zarezadeh; A. Mirhossaini; M. Mirza; Z. Jamzad; M. Arabzadeh
Abstract
Thymuskotschyanus Boiss. & Hohen. is a perennial medicinal shrub growing wild in some regions of Iran including Yazd province. This research was carried out in order to analyze oil quantity and quality of cultivated Thymuskotschyanus in the Medicinal Plants Farm, Yazd, Iran. The seeds of 19 accessions ...
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Thymuskotschyanus Boiss. & Hohen. is a perennial medicinal shrub growing wild in some regions of Iran including Yazd province. This research was carried out in order to analyze oil quantity and quality of cultivated Thymuskotschyanus in the Medicinal Plants Farm, Yazd, Iran. The seeds of 19 accessions collected from natural habitats were sown in a greenhouse and the seedlings were planted in the farm. Shoots were harvested at 50% flowering stage in the second year and dried under shadow. Essential oil was extracted with water distillation method and oil components were identified after determining oil content. The highest amounts of oil content respectively were related to accessions TK7 (3.42%), TK12 (3.22%), TK17 (2.66%) and the lowest amount was observed in accession TK6 (0.79%). The highest amounts of oil production respectively were recorded in accessions TK5 (38.3kg/ha), TK7 (36.4kg/ha) and TK12 (36.1 kg/ha) and the lowest amount was related to TK1 (1.3 kg/ha). Cluster analysis based on eleven oil components classified the accessions into three groups. The first group, (TK2, TK6, TK13, TK18, TK16, TK19), was rich for thymol-p-cymene, the scond group (TK1, TK3, TK4, TK5, TK7, TK11, TK14, TK15, TK17) for carvacrol-thymol and the 3rd group (TK8, TK9, TK10, TK12) was rich for Geraniol-linalool. In general, accessions TK7, with 3.42% oil content, 36.4 kg/ha oil production and 63.8% carvacrol; TK14, with 2.2% oil content 35.5 kg/ha oil production and 47.2% carvacrol and thymol, TK5 with 2.4% oil content, 38.3 kg/ha oil production and 57% thymol and carvacrol are proposed as superior accessions.
A Salimi; V. Rowshan; E. Khanpoor
Abstract
This research was aimed to investigate the effect of salinity on quality and quantity of essential oil components and antioxidant activity in Achillea millefolium L. The study was conducted in a completely randomized design in greenhouse, controlled environment, with four treatments including 0 (control), ...
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This research was aimed to investigate the effect of salinity on quality and quantity of essential oil components and antioxidant activity in Achillea millefolium L. The study was conducted in a completely randomized design in greenhouse, controlled environment, with four treatments including 0 (control), 50, 100 and 150 mM NaCl and four replications per treatment. The essential oil was first extracted and the active components of the essential oil were separated and identified by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) methods. Then, the proline content in fresh leaf and antioxidant activity of methanolic extract were determined. According to the results, the essential oil yield showed a significant increase at 150 mM NaCl. Regarding the essential oil composition, the main compounds were delta-cadinol, trans-beta-farnesene, α-Bisabolol, borneol, caryophyllene oxide, and menthone, showing an increase with increasing salinity. Some compounds such as sabinene, cis-β-ocimene, cis-γ-bisabolene were only detected in control treatment. Menthone, beta-bisabolene, epi-beta-santalene, and 1,8-cineole were only detected in the plants treated with salt. Proline content was increased with increased concentration of NaCl. The results showed that radical scavenging activity and reducing power of yarrow extract increased with increasing of salinity concentration. Our findings suggest that salt stress increases the antioxidant compounds in Achillea millefolium.
M. Pirooz; H. Amiri; B. Dostii
Abstract
Thymus daenensis Celak., belonging to the Lamiaceae family, is an endemic species of genus Thymus in Iran. Despite the increasing need for mass propagation of this plant due to the presence of useful secondary metabolites, there is little information about its proliferation. In the present study, the ...
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Thymus daenensis Celak., belonging to the Lamiaceae family, is an endemic species of genus Thymus in Iran. Despite the increasing need for mass propagation of this plant due to the presence of useful secondary metabolites, there is little information about its proliferation. In the present study, the interaction of some hormonal treatments in the MS medium containing indole acetic acid (IAA), benzyl amino purine (BAP) and kinetin (Kin) in presence or absence ascorbic acid (AS) were investigated for callus induction and micropropagation of Thymus daenensis. Results showed that the callus index, fresh weight, and dry weight of callus from internode explants were better as compared with apical meristem explants. Significant differences were found for regeneration from callus cultures derived from apical meristem and internode, so that NAA-Kin (4.5 and 1.5 mg/L) and NAA-BAP (9 and 6 mg/L) were the best hormonal combination. As well, significant differences were found between callus production from internode explants and apical meristem explants, so that Kin-NAA (9 and 3 mg/L), and NAA-BAP (6 and 9 mg/L) in presence of ascorbic acid were the best hormonal combination. Overall, the control treatment, NAA and Kin showed favorable effects on callus induction and better regeneration was obtained from internode explants.
S. Khalesro; H. Malekian
Abstract
Nowadays, using organic inputs instead of chemical fertilizers is the most important point for achieving soil productivity and suitable plant nutrition in organic farming. The application of these inputs not only protects the environmental health, but also improves the qualitative characteristics of ...
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Nowadays, using organic inputs instead of chemical fertilizers is the most important point for achieving soil productivity and suitable plant nutrition in organic farming. The application of these inputs not only protects the environmental health, but also improves the qualitative characteristics of medicinal plants. The purpose of this research was to investigate the effects of vermicompost and humic acidon morphological traits, yield, essential, oil content and component of Ajowan (Trachyspermum ammi L.). Thus, a field experiment was conducted in the Agriculture Research Station of Kurdistan University in 2013. The treatments consisted of three level of vermicompost (0, 5 and 10 t.ha-1) and three levels of humic acid (0, 0.5 and 1 percent). The study was conducted in a randomized complete blocks design with factorial arrangement and three replications. The results showed that the highest plant height and number of umbels/plant were obtained at the third level of vermicompost. The third level of humic acid caused the highest values of plant height and number of umbels/plant, as well. The highest number of umbel /plant, seed and biological yield and essential oil percentage were obtained with applying the third level of vermicompost and humic acid. Hence, it seems that organic fertilizers such as vermicompost and humic acid could be applied as a proper alternative to improve the qualitative and quantitative yield of Ajowan.
abbas zarezadeh; Fatemeh Sefidkon; S.R. Tabaei-Aghdaei; A. Mirhossaini; M.R. Arabzadeh; M.R. Mijalili
Abstract
Savory (Satureja) consists of 17 herbaceous annual and perennial species in Iran and Satureja rechingeri Jamzad is one of the endemic species in this country. This research was carried to study oil quantity and quality in S. rechingeri. Seeds of eight accessions, collected from natural habitats, were ...
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Savory (Satureja) consists of 17 herbaceous annual and perennial species in Iran and Satureja rechingeri Jamzad is one of the endemic species in this country. This research was carried to study oil quantity and quality in S. rechingeri. Seeds of eight accessions, collected from natural habitats, were sown in a greenhouse and the seedlings were planted in the Medicinal Plants Farm, Yazd, Iran. Essential oil was extracted from shadow dried flowering shoots with water distillation method and oil components were identified after determining oil content. Cluster analysis based on oil components classified the accessions into two groups, one of which was rich in carvacrol (SR1, SR2, SR3, SR5, SR7, SR8) and the other was rich in both carvacrol and thymol (SR6 and SR4). Based on the results of this study, SR4 with 5.85% essential oil, 113.9kg/ha oil yield, 64% carvacrol and 12.2% thymol , and SR6 with 4.22% essential oil, 63.8 8kg/ha oil yield, 75% carvacrol and 6.2% thymol are proposed as superior accessions.
A. Sepahvand; K. Khademi; H. Astereki; A. Mohammadian
Abstract
This research was aimed to study the effects of density on yield and yield components of thyme (Thymus lancifolius Celak.) under dry farming conditions of Lorestan province. The experiment was arranged in a randomized complete blocks design with three sowing density treatments (4, 6, and 8 plants per ...
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This research was aimed to study the effects of density on yield and yield components of thyme (Thymus lancifolius Celak.) under dry farming conditions of Lorestan province. The experiment was arranged in a randomized complete blocks design with three sowing density treatments (4, 6, and 8 plants per square meter) and three replications. The plant height, crown diameter, number of shoots, shoot dry yield, root length, and essential oil yield and content were measured. The results of combined analysis showed that the highest number of branches was obtained at a density of 8 plants per square meter as compared with 4 and 6 plants per square meter. According to the results, the dry matter yield and essential oil yield and content were calculated to be 3937.9 kg ha-1, 77.52 kg, and 1.973%, respectively. In addition, in the fourth year of study, the number of flowering branches, crown diameter and height were calculated to be 317.153, 42.87 cm and 15.3cm, respectively. The essential oil T. lancifolius contained more than 44% carvacrol and 31% thymol.
L. Elyasi; A.A. Mehrabi; M. Seyedi; Z. Safari
Abstract
Satureja bachtiarica L., belonging to the Lamiaceae family, is rich in essential oils, used for various purposes including pharmaceutical, food, and health applications. In vitro culture of Satureja, in order to callus induction and optimization of cell suspension, provides rapid production and easy ...
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Satureja bachtiarica L., belonging to the Lamiaceae family, is rich in essential oils, used for various purposes including pharmaceutical, food, and health applications. In vitro culture of Satureja, in order to callus induction and optimization of cell suspension, provides rapid production and easy extraction of secondary metabolites. In the present study, callus induction was conducted by using different explants and concentrations of growth regulators. The most suitable calli were selected to induce suspension cultures and were transferred to liquid media supplemented with different combination of BAP and NAA. The experiment was performed in a factorial completely randomized design by using three replications. The cell biomass (cell number) was examined on different days. The mean comparison results for the explant origin showed that the internode explant produced the highest cell number. In addition, evaluation of interaction of explants with media showed that the maximum cell biomass was obtained by internode explant and the medium containing 2mg.L-1 NAA plus 0.5mg.L-1 BAP in the fifth day. On the other hand, study of growth process and cell proliferation during the days after the establishment of suspension cultures, also revealed that this hormonal composition had the highest utility to generate the maximum number of cells in all explants tested.
H. Yadegari; I. Khammari; M. Salari; B.A. Fakheri; M. Rahimi; F. Bidarnamani
Abstract
Milk thistle (Silybum marianum L.), belonging to the Asteraceae family, is an annual plant and prefers light and fertile soils. Silymarin is the most important compound extracted from the seeds of this plant and it is used in the treatment of liver diseases, hepatitis, cirrhosis and liver cancer prevention. ...
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Milk thistle (Silybum marianum L.), belonging to the Asteraceae family, is an annual plant and prefers light and fertile soils. Silymarin is the most important compound extracted from the seeds of this plant and it is used in the treatment of liver diseases, hepatitis, cirrhosis and liver cancer prevention. In usual agricultural systems, the purpose of using fertilizers and their combination is to obtain the highest seed yield and quality of the active substances. In order to investigation of the effect of different fertilizers and their combination on some quantitative and qualitative characteristics of milk thistle, an experiment was arranged in split plot in a randomized complete block design with three replications in the Research Farm of Agricultural Institute, University of Zabol, during 2013-2014. In this study, the main factors were Hungary genotype (A1) and Mashhadi population (A2) and the sub factors were application of different fertilizers such as control treatment, manure, chemical fertilizer (urea + ammonium phosphate + potassium), mycorrhizal fungi (Glomus mossaea), combination of nitroxin, bio-sulfur and bio-superphosphate, combination of chemical fertilizer and manure, combination of mycorrhizal fungi and manure, and nano-iron chelate. The results showed that there was not any significant difference between Hungary genotype and Mashhadi population. Totally, the mycorrhizal fungi (G. mossaea) was the best treatment than other fertilizer treatments in the milk thistle plant and it caused enhancing the growth and better activity of photosynthetic pigments as well as improved seed yield and increased silymarin substance in the milk thistle plant.
Y. Behzadi; A. Salehi
Abstract
The aim of this study was to determine the effects of biological, organic, and chemical fertilizers on the uptake of nitrogen (N), phosphorus (P), potassium (K), grain yield, and essential oil in anisum (Pimpinella anisum L.). The experiment was carried out in the agricultural research field of Yasouj ...
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The aim of this study was to determine the effects of biological, organic, and chemical fertilizers on the uptake of nitrogen (N), phosphorus (P), potassium (K), grain yield, and essential oil in anisum (Pimpinella anisum L.). The experiment was carried out in the agricultural research field of Yasouj University in 2013, as a randomized complete block design with three replications. The treatments consisted of pure vermicompost (zero, 5 and 10 ton.ha-1), biofertilizer (Azotobacter, Barvar-1), biological phosphate fertilizer (Barvar-2) and the mixed Barvar-1 and Barvar-2, urea fertilizer, nano-biofertilizers and combined treatments of vermicompost (2.5, 5 and 7.5 ton.ha-1) with biological fertilizers Barvar-1,2 and the mixed Barvar-1,2. Results showed that the maximum nitrogen (1.390%) phosphorus (0.290%) potassium (1.866%) and chlorophyll a+b (0.410 ml.g-1) were obtained by the application of vermicompost at 10ton.ha-1. Also, maximum grain yield (595.90 kg.h-1) and essential oil yield (16.60 kg.h-1) were obtained by the application of vermicompost at 10ton.ha-1, showing no significant difference with combined treatment of vermicompost at 7.5 ton.ha-1 and Barvar-1,2. The minimum values for the study traits were obtained from the control treatment. According to the results, it appears that the application of 7.5 ton.ha-1 vermicompost is the best treatment to produce anise grain yield in organic system.
R. Jafari Hajati; V. Payamnoor; K. Ghasemi Bezdi; N. Ahmadian Chashmi
Abstract
This study aimed to enhance the production of betulin and betulinic acid using suspension cultures of birch (Betula pendula Roth) and elicitation of the cell cultures by methyl jasmonate (MeJA) and salicylic acid (SA). To do this, at the first step, the cell growth curve was investigated in a 16-day ...
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This study aimed to enhance the production of betulin and betulinic acid using suspension cultures of birch (Betula pendula Roth) and elicitation of the cell cultures by methyl jasmonate (MeJA) and salicylic acid (SA). To do this, at the first step, the cell growth curve was investigated in a 16-day period. Then, two elicitors, namely, MeJA (at final concentration of 0, 50, 100, 150 and 200 µM) and SA (at final concentration of 0, 100, 200, 300 and 400 µM) were separately supplemented to 8-day-old cell cultures and the cells were harvested 1, 2, 3, 5 and 7 days after elicitations. Fresh weight (FW), dry weight (DW) and cell viability were measured. In addition, betulin and betulinic acid content were analyzed using HPLC. The results showed the significant effects of different concentrations of SA and MeJA on metabolites content and FW and DW. Maximum amount of betulin was observed about 4-fold (2.5 mg g-1 DW) higher than the control treatment by addition of 100 µM SA, two days after elicitation. Moreover, betulinic acid content was enhanced about 5 mg g-1 DW, 4.5-fold compared to control, one day after addition of 200 µM SA. Furthermore, the high accumulation of betulin (2.3 mg g-1 DW) was obtained in the elicited cell by 50 µM MeJA, seven days after elicitation. Also, the maximum amount of betulinic acid, about 3 mg g-1 DW, was observed in the cells elicited by 100 µM MeJA, two days after elicitation. Overall, the effect of SA on the production of betulin and betulinic acid was significantly more than the effect of MeJA.
S.F. Taghizadeh; H. Aroiee; J. Asili
Abstract
This study was conducted to determine the effects of saline stress and coronatine on physiological and biochemical characteristics of two berberis cultivars (Berberis crataegina DC. and Berberis integerrima Bge.) in 2013. The study was arranged in a factorial completely randomized design (RCD) with four ...
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This study was conducted to determine the effects of saline stress and coronatine on physiological and biochemical characteristics of two berberis cultivars (Berberis crataegina DC. and Berberis integerrima Bge.) in 2013. The study was arranged in a factorial completely randomized design (RCD) with four replications. The study species were planted in the pots containing sand. The plants were treated with NaCl at four levels of 0, 25, 50, 75mM and three levels of coronatine (0. 0.75, 1.5mM). The results showed that the content of proline, total phenols, total flavonoids, total proanthocyanidine and anthocyanin increased in plants treated with salinity stress while the chlorophyll content was reduced by increasing of salt concentration. At the same concentration of NaCl, the maximum content of proline was recorded in plants treated with coronatine (1.5mM). The highest increase in the total chlorophyll content was related to coronatine (0.75mM); however, the content of total phenols, total flavonoids, total proanthocyanidine and anthocyanin was decreased at a concentration of 1.5mM coronatine.
M.T. Darzi; M.R. Haj Seyed Hadi
Abstract
In order to study the effects of organic and bio-fertilizers on some quantitative and qualitative characters of dragonhead (Dracocephalum moldavica L.), an experiment was conducted as randomized complete blocks design with eight treatments and three replications. The study was conducted in the Research ...
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In order to study the effects of organic and bio-fertilizers on some quantitative and qualitative characters of dragonhead (Dracocephalum moldavica L.), an experiment was conducted as randomized complete blocks design with eight treatments and three replications. The study was conducted in the Research Field Station of Ran Agricultural Company in Firouzkuh, 2015. The treatments were 20 t/ha manure, 10 t/ha vermicompost, biofertilizer (Nitroxin + Bio Super Phosphat), 10 t/ha manure + 5 t/ha vermicompost, 20 t/ha manure + biofertilizer, 10 t/ha vermicompost + biofertilizer, 10 t/ha manure + 5 t/ha vermicompost + biofertilizer and chemical fertilizer (NPK: 80, 70 and 80 kg/ha). The results showed that treatments had significant effects on the traits studied except plant height, so that the highest flowering shoot no./plant, flower dry weight, plant dry weight and dry flowering yield were obtained with integrated application of 10 t/ha manure and 5 t/ha vermicompost. In addition, the highest essential oil content and geraniol in the essential oil were obtained in the treatment of application of 10 t/ha vermicompost.
A. Ataei Azimi; B. Delnavaz Hashemloian; M. Salimi; A.R. Oman; A. Nazemi; A. Eghdami
Abstract
Artemisia annua L. and Artemisia scoparia Waldst. & Kit. are two important medicinal plants, distributed in many parts of the world. Malaria is an infectious disease in humans and animals caused by various species of the genus Plasmodium, protozoan parasite begins. In this study, artemisinin and ...
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Artemisia annua L. and Artemisia scoparia Waldst. & Kit. are two important medicinal plants, distributed in many parts of the world. Malaria is an infectious disease in humans and animals caused by various species of the genus Plasmodium, protozoan parasite begins. In this study, artemisinin and terpenoids were extracted from Artemisia annua and Artemisia scoparia. The terpenoids and artemisinin content was measured by spectrophotometry methods. The terpenoids were detected by Thin-Layer Chromatography and Mass Gas Chromatography. The antimalarial effects of the study extracts were measured based on the inhibitory effect of the extracts on in vitro formation of β-hematin (hemazoin). According to the results, it seemed that all extracts contained artemisinin. However, the results of Thin-Layer Chromatography (TLC) and Mass Gas Chromatography (GC-Mass) showed that the acetonitrile extract (artemisininic extract) of Artemisia annua contained artemisinin and several kinds of terpenoids. The extract of A. scoparia was lack of artemisinin despite containing lots of terpenoids. The inhibitory effects of the study extracts on the in vitro formation of β-hematin (hemazoin) showed that the acetonitrile and aquatic extracts of the shoots and roots of A. annua and A. scoparia had antimalarial effects. The inhibitory effects of acetonitrile extract of A. annua shoots was double of the artemisininic extract of A. scoparia shoots and the aquatic extract of A. annua shoots and roots. The acetonitrile extract of A. annua contained artemisinin. The inhibitory effect of other extracts on the in vitro formation of β-hematin (hemozoin) showed the antimalarial effects of other terpenes in the extracts. The inhibitory effect of all extracts, except the aqueous extract of shoots and the acetonitrile extract of roots in A. scoparia, was more than that of chloroquine phosphate.
F. Ghasemi; A. Baghizadeh; Gh. Mohammadinejad; H.R. Kavoosi
Abstract
The selection indices is one of the most effective methods for improving complex traits such as yield. In the current study, 50 genotypes of cumin (Cuminum cyminum L.) were evaluated to assess the efficiency of different selection indices. The experiment was conducted in a randomized complete block design ...
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The selection indices is one of the most effective methods for improving complex traits such as yield. In the current study, 50 genotypes of cumin (Cuminum cyminum L.) were evaluated to assess the efficiency of different selection indices. The experiment was conducted in a randomized complete block design whit three replications in the Research Farm of Shahid Bahonar University, Kerman, Iran in 2011-2012. The Smith-Hazel and Pesek-Baker indices were evaluated based on seven traits including plant height, number of branches, number of umbels/plant, number of umbelets/plant, number of seeds/plant, seed yield/plant and shoot weight (with and without seed). The results of response to selection and relative selection efficiency indicated that the genotypes with higher number of seeds and branches/plant and lower plant height had the highest yield potential. In the Smith-Hazel index, number of branches and umbelets/plant had the highest response to selection, while in the Pesek-Baker index, the number of umbels/plant had the highest response to selection. Our results indicated that the Smith-Hazel index had the most selection efficiency and could be used in cumin breeding programs.
F. Yousefi; N. Hasanzadeh
Abstract
This research was aimed to study the effects of essential oil of Lavandula (Lavandula angustifolia Mill.) flowers, fennel (Foeniculum vulgare Miller.) and cumin (Cuminum cyminum L.) seeds, and peppermint (Mentha piperita L.) shoots on the fungus Botrytis cinerea, causing strawberry gray mold. The study ...
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This research was aimed to study the effects of essential oil of Lavandula (Lavandula angustifolia Mill.) flowers, fennel (Foeniculum vulgare Miller.) and cumin (Cuminum cyminum L.) seeds, and peppermint (Mentha piperita L.) shoots on the fungus Botrytis cinerea, causing strawberry gray mold. The study was conducted in PDA medium. The results showed that the essential oils of fennel and cumin had the highest antifungal activity. The essential oil compounds were isolated and identified by GC and GC/MS. The results obtained for the fruits inoculated with a spore suspension (1×105 spores in ml) indicated that the cumin oil was more effective in controlling the fungus B. cinerea on strawberry fruits as compared with fennel oil. The cumin oil had the highest effect in controlling strawberry gray mold, placed in one statistical group with thiabendazole.
