In collaboration with Scientific Association of Iranian Medicinal Plants

Document Type : Research Paper

Authors

1 M.Sc. Student, Department of Horticulture, University of Tehran, Karaj, Iran

2 Department of Horticulture, University of Tehran, Karaj, Iran

3 Facultat de Farmacia, Universitat de Barcelona, Barcelona, Spain

Abstract

GenusSalvia, belonging to the Labiatae family, has valuable active ingredients including essential oils, flavonoids and rosmarinic acid. Pharmacological researches on Salvia genus have confirmed medicinal properties of the plant including antibacterial and antioxidant effects. Total flavonoids, rosmarinic acid and the content and chemical compounds of essential oil of three populations of Salvia reuterana Boiss., collected from three regions of Iran (Deli chai, Kaswa and Shahmirzad), as well as Salvia palaestina Benth., collected from Rodbarak, were evaluated in present study. Fifty-six compounds of S. reuterana and S. palaestina were identified by GC-MS. The most important chemical compositions for S. reuterana were α-gurjunen, β-elemene, germacern-D, N-hexyl acetate and spatholenol and for S. palaestina were caryophyllen, dihydro carveol, germacern-D, linalool and spatholenol. Diethyl ether extract of leaves was used for quantification and identification of flavonoids and rosmarinic acid. The crushed leaves of the plant were dissolved in diethyl ether solvent and then were injected into the LC-DAD-ESI-MS system. Total flavonoids amount of S. reuterana, collected from Kaswa, Deli chai and Shahmirzad were calculated to be 3252.76, 3697.19 and 5132.92 µg/ g, respectively. Also the amount of rosmarinic acid in plants, collected from Kaswa, Deli chai and Shahmirzad was 122, 177.11 and 98.46 µg/ g, respectively. S. palaestina Rodbarak showed 3808 and 19.72 µg/g of total flavonoids and rosmarinic acid, respectively.

Keywords

- مظفریان، و.ا.، 1375. فرهنگ نامهای گیاهان ایران. انتشارات فرهنگ معاصر، تهران، 477 صفحه.
- AI-Howiriny, T.A., 2007. Composition and anti-microbial activity of the essential oil of Salvia palaestina growing Saudi Arabia. Saudi pharmaceutical Journal, 15: 218-223.
- Amiri, H., Meshkat Al Sadat, M.H., Lari Yazdi, H. and Goodarzi, A., 2006. Essential oil composition of Salvia reuterana Boiss. Iranian Journal of Medicinal and Aromatic Plants, 22(3): 270-275.
- Bisht, A., 2011. Chemical composition and antimicrobial activity of essential oil of tubers of Cyperus rotundus Linn. collected from Dehradun (Uttarakhand). International Journal of Research in Pharmaceutical and Biomedical Sciences, 2(2): 661-665.
- Edris, A.E., 2009. Anti-cancer properties of Nigella spp. essential oils and their major constituents, thymoquinone and beta-elemene. Current Clinical Pharmacology, 4: 43-46.
- Fattahi, M., Nazeri, V., Sefidkon, F., Zamani, Z., Palazon, J., Mercedes, B. and Cusido, R., 2011. Screening of Dracocephalum kotschyi accession for surface flavonoids and rosmarinic acid. Planta Medica, 77(12): 1385-1385.
- Gohari, A.R., Saeidnia, S., Malmir, M., Hadjiakhoondi, A. and Ajani, Y., 2010. Flavones and rosmarinic acid from Salvia limbata. Natural Product Research, 24(20): 1902-1906.
- Greenham, J., Harborne, J.B. and Williams, C.A., 2003. Identification of lipophilic flavones and flavonols by comparative HPLC, TLC and UV spectral analysis. Phytochemical Analysis, 14(2): 100-118.
- Miguel, G., Cruz, C., Faleiro, M.L., Simoes, M.T.F., Figueiredo, A.C., Barroso, J.G. and Pedro, L.G., 2011. Salvia officinalis L. essential oils: effect of hydrodistillation time on the chemical composition, antioxidant and antimicrobial activities. Natural Product Research: Formerly Natural Product Letters, 25(5): 526-541.
- Mirza, M. and Sefidkon, F., 1999. Essential oil composition of two Salvia species from Iran, Salvia nemorosa L. and Salvia reuterana Boiss. Flavour and Fragrance Journal, 14(4): 230-232.
- Nickavar, B., Abolhasani, L. and Izadpanah, H., 2008. α-Amylase inhibitory activities of six Salvia species. Iranian Journal of Pharmaceutical Research, 7(4), 297-303.
- Parnham,M.J. and Kesselring, K., 1985. Rosmarinic acid. Drugs Future,10: 756-757.
- Rechinger, K.H., 1982. Flora Iranica. No.150, Graz: Akademisch Druck-u.Verlagsanstal, 462p.
- Salehi, P., Sefidkon, F., Bazzaz tolami, L. and Sonboli, A., 2005. Essential oil composition of Salvia palaestina Benth. from Iran. Flavour and Fragrance Journal, 20(5): 525-527.
- Tomás-Barberán, F.A. and Wollenweber, E., 1990. Flavonoid aglycones from the leaf surfaces of some Labiatae species. Plant Systematics and Evolution, 173(3): 109-118.